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The map label for this gene is mtnE [H]

Identifier: 226222922

GI number: 226222922

Start: 317288

End: 318469

Strand: Direct

Name: mtnE [H]

Synonym: Lm4b_00314

Alternate gene names: 226222922

Gene position: 317288-318469 (Clockwise)

Preceding gene: 226222916

Following gene: 226222923

Centisome position: 10.89

GC content: 40.27

Gene sequence:

>1182_bases
ATGAAAATTTCCAAACGCTTGCAAAATTTACCAGATCAGTTTTTCTCTAGCCTTGTTGAAAAAGTTGGAAAAAAGGTGGC
AGAAGGGCATGATGTCATTAATTTAGGACAAGGAAACCCAGACCAGCCAACACCCAAACATATTGTGGAAGCAATGAAAA
CAGCTTCAGAAAAACCACTTAACCACAAATATTCTTTATTTCGAGGAAAACACGAACTAAAACAAGCCGCTGCGGATTTT
TACGCGCGTGAATATAATGTAACAATTGATCCAAACACGGAAGTAGCTATTTTGTTTGGGACAAAAACAGGTTTAGTGGA
GCTGCCGATGTGTTTGATGGATCCAGGTGATACGATGCTTTTACCTGATCCGGGCTATCCTGATTATTTGTCAGGAGTAG
TGTTAGGTGAAGTTCAATTTGAAAAAATGCCGCTTATTGCCGCAAATAATTTTTTACCAGATTTCACTAAAATTCCAGAA
GACATAGCTGAAAAAGCTGAATTAATGTATTTAAATTATCCTAATAATCCAACTGGCGCTGTGGCTACGGCAGATTTCTT
TGAAGAAACAGTCGCTTTTGCCAAGAATCATAATATCGTTGTTGCGCACGATTTCGCATATGGAGGTATTGGCTTTGACG
GTAAAAAACCAATTAGTTTTTTAGAAACAAACGGTGCAAAAGAAGTTGGAATTGAACTTTATACACTTTCGAAAACATAT
AATATGGCTGGCTGGCGCGTTGGTTTTGCAGTTGGGAACAGCGAAGTTATTGAAGCTATTAATCTTATTCAAGATCATAT
GTATGTAAGTCTTTTTCCAGGGATTCAAGATGCTGCAATCGAAGCTTTAACAGGAGATCAGACTTGTGTGGGTGAATTAA
CCGCTCGTTATGAAAGTCGCAGGGATGCCTTTATTTCTGCTTGTAAGAAAATTGGTTGGGAGGCAGTTGCGCCGGCGGGT
TCGTTCTTTGCTTGGATGCCAGTCCCGGAAAATTTTACAAGTAGCGAATTTGCTGATTATCTGCTAGAAGAGGTGAGTGT
TGCCGTTGCGGATGGTAGTGGCTTTGGGGAGTTTGGTGAAGGTTACGTCCGCGTAGGTCTTTTAATGGATGAAGAGCGCT
TGGAAGAAGCAGTAACGCGAGTTTCCAAATTGCATTTGTTCGATAAAGTTACACAGCAGTAG

Upstream 100 bases:

>100_bases
TTGTCAATGCTTTTTTAAGTTTTAAAAAAATCATACTGGACAAATACTTCAGAAAACTTTACTATTTTAATCAATGAAAC
TTGAAAAGGACGGGTGTAAA

Downstream 100 bases:

>100_bases
CCAATTTATCCAATTTTAAGAGAAAATCGCTTGTAATAATTCAGATAATTAATGGGATGTGTTATAATTGGTTAGTAAGT
CTATTCTTAGCATGTTGTTT

Product: transaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVEAMKTASEKPLNHKYSLFRGKHELKQAAADF
YAREYNVTIDPNTEVAILFGTKTGLVELPMCLMDPGDTMLLPDPGYPDYLSGVVLGEVQFEKMPLIAANNFLPDFTKIPE
DIAEKAELMYLNYPNNPTGAVATADFFEETVAFAKNHNIVVAHDFAYGGIGFDGKKPISFLETNGAKEVGIELYTLSKTY
NMAGWRVGFAVGNSEVIEAINLIQDHMYVSLFPGIQDAAIEALTGDQTCVGELTARYESRRDAFISACKKIGWEAVAPAG
SFFAWMPVPENFTSSEFADYLLEEVSVAVADGSGFGEFGEGYVRVGLLMDEERLEEAVTRVSKLHLFDKVTQQ

Sequences:

>Translated_393_residues
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVEAMKTASEKPLNHKYSLFRGKHELKQAAADF
YAREYNVTIDPNTEVAILFGTKTGLVELPMCLMDPGDTMLLPDPGYPDYLSGVVLGEVQFEKMPLIAANNFLPDFTKIPE
DIAEKAELMYLNYPNNPTGAVATADFFEETVAFAKNHNIVVAHDFAYGGIGFDGKKPISFLETNGAKEVGIELYTLSKTY
NMAGWRVGFAVGNSEVIEAINLIQDHMYVSLFPGIQDAAIEALTGDQTCVGELTARYESRRDAFISACKKIGWEAVAPAG
SFFAWMPVPENFTSSEFADYLLEEVSVAVADGSGFGEFGEGYVRVGLLMDEERLEEAVTRVSKLHLFDKVTQQ
>Mature_393_residues
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVEAMKTASEKPLNHKYSLFRGKHELKQAAADF
YAREYNVTIDPNTEVAILFGTKTGLVELPMCLMDPGDTMLLPDPGYPDYLSGVVLGEVQFEKMPLIAANNFLPDFTKIPE
DIAEKAELMYLNYPNNPTGAVATADFFEETVAFAKNHNIVVAHDFAYGGIGFDGKKPISFLETNGAKEVGIELYTLSKTY
NMAGWRVGFAVGNSEVIEAINLIQDHMYVSLFPGIQDAAIEALTGDQTCVGELTARYESRRDAFISACKKIGWEAVAPAG
SFFAWMPVPENFTSSEFADYLLEEVSVAVADGSGFGEFGEGYVRVGLLMDEERLEEAVTRVSKLHLFDKVTQQ

Specific function: Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) (Probable) [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily [H]

Homologues:

Organism=Homo sapiens, GI95147551, Length=317, Percent_Identity=25.2365930599369, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI169881279, Length=317, Percent_Identity=25.2365930599369, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI56713254, Length=316, Percent_Identity=24.3670886075949, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56713256, Length=316, Percent_Identity=24.0506329113924, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI169881281, Length=306, Percent_Identity=23.202614379085, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1788722, Length=386, Percent_Identity=37.0466321243523, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI1786816, Length=364, Percent_Identity=25.8241758241758, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788627, Length=402, Percent_Identity=24.1293532338308, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994472, Length=388, Percent_Identity=25, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71994476, Length=388, Percent_Identity=25, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17567369, Length=318, Percent_Identity=22.9559748427673, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322401, Length=395, Percent_Identity=22.0253164556962, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28573069, Length=416, Percent_Identity=22.1153846153846, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24646114, Length=416, Percent_Identity=22.1153846153846, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28573067, Length=416, Percent_Identity=22.1153846153846, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28573065, Length=416, Percent_Identity=22.1153846153846, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.- [C]

Molecular weight: Translated: 43309; Mature: 43309

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVEAMKTASEKPL
CCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCC
NHKYSLFRGKHELKQAAADFYAREYNVTIDPNTEVAILFGTKTGLVELPMCLMDPGDTML
CCHHHHHHCHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCHHHCCHHHCCCCCEEE
LPDPGYPDYLSGVVLGEVQFEKMPLIAANNFLPDFTKIPEDIAEKAELMYLNYPNNPTGA
ECCCCCCHHHCCEEEEEEEHHHCCEEEECCCCCCHHHCCHHHHCCEEEEEEECCCCCCCC
VATADFFEETVAFAKNHNIVVAHDFAYGGIGFDGKKPISFLETNGAKEVGIELYTLSKTY
EEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHEECCCCHHHCEEEEEEECCC
NMAGWRVGFAVGNSEVIEAINLIQDHMYVSLFPGIQDAAIEALTGDQTCVGELTARYESR
CCCCEEEEEEECCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHH
RDAFISACKKIGWEAVAPAGSFFAWMPVPENFTSSEFADYLLEEVSVAVADGSGFGEFGE
HHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCC
GYVRVGLLMDEERLEEAVTRVSKLHLFDKVTQQ
CEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVEAMKTASEKPL
CCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCC
NHKYSLFRGKHELKQAAADFYAREYNVTIDPNTEVAILFGTKTGLVELPMCLMDPGDTML
CCHHHHHHCHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCHHHCCHHHCCCCCEEE
LPDPGYPDYLSGVVLGEVQFEKMPLIAANNFLPDFTKIPEDIAEKAELMYLNYPNNPTGA
ECCCCCCHHHCCEEEEEEEHHHCCEEEECCCCCCHHHCCHHHHCCEEEEEEECCCCCCCC
VATADFFEETVAFAKNHNIVVAHDFAYGGIGFDGKKPISFLETNGAKEVGIELYTLSKTY
EEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHEECCCCHHHCEEEEEEECCC
NMAGWRVGFAVGNSEVIEAINLIQDHMYVSLFPGIQDAAIEALTGDQTCVGELTARYESR
CCCCEEEEEEECCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHH
RDAFISACKKIGWEAVAPAGSFFAWMPVPENFTSSEFADYLLEEVSVAVADGSGFGEFGE
HHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCC
GYVRVGLLMDEERLEEAVTRVSKLHLFDKVTQQ
CEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]