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The map label for this gene is purA

Identifier: 226222700

GI number: 226222700

Start: 65075

End: 66367

Strand: Direct

Name: purA

Synonym: Lm4b_00064

Alternate gene names: 226222700

Gene position: 65075-66367 (Clockwise)

Preceding gene: 226222699

Following gene: 226222701

Centisome position: 2.23

GC content: 39.91

Gene sequence:

>1293_bases
ATGTCTTCAGTTGTTGTAGTAGGAACACAGTGGGGCGATGAAGGAAAAGGGAAGATTACAGATTTTCTTTCCGAAAATGC
AGAAGCGATTGCTAGATATCAAGGTGGGAACAATGCAGGCCATACAATTAAGTTTGATGGCGTAACGTACAAATTACACT
TAATTCCATCAGGTATCTTTTACAAAGAAAAAATTAGTGTTATTGGTAACGGTATGGTTGTGGATCCAAAAGCTTTAGTG
GAGGAATTAAAATATCTTCATGACAAAGGCGTGGATACTTCTAATTTGCGTATTTCTAACCGTGCGCACATTATTTTACC
ATATCACATTCGTATTGATGAAGCTGACGAAGAACGTAAGGGCGCAAACAAAATCGGTACAACGAAAAAAGGTATTGGCC
CAGCTTACATGGATAAAGCAGCGCGTGTAGGTATTCGTATTATTGATTTATTAGATAAAGAAACATTTAAAGAGAAATTA
GAGCATAATCTTGGCGAGAAAAACCGTTTATTAGAACGTTTTTACGAACTAGAAGGCTTTAAACTAGAAGATATTTTAGA
AGAATATTATGACTATGGTCAACAATTTAAAGAATATGTTTGCGATACATCTGTTGTTTTAAATGACGCATTAGATGATG
GTAAACGTGTATTATTTGAAGGTGCGCAAGGGGTTATGCTTGATATTGATCAAGGAACATATCCATTTGTAACTTCAAGT
AACCCGATTGCTGGTGGCGTAACTATCGGTAGTGGCGTAGGTCCTTCAAAAATCAATCATGTTGTTGGTGTGGCGAAAGC
TTATACAACACGTGTTGGTGATGGTCCTTTCCCAACAGAATTATTCGATACTATTGGGGACACTATCCGTGAGGTTGGTC
ATGAGTATGGTACAACGACTGGTCGTCCGCGCCGTGTAGGTTGGTTTGACAGTGTAGTGGTTCGCCATGCGCGTCGTGTT
AGTGGCTTAACAGATTTATCGTTAACACTACTGGATGTTTTGACAGGAATTGAAACACTTAAAATCTGCGTAGCTTACAA
ATTAGACGGAAAAACAATTACAGAGTTCCCGGCAAGTCTGAAAGATCTAGCTCGTTGCGAACCTGTATATGAAGAACTTC
CAGGCTGGACGGAAGATATTACTGGAGTTACATCACTCGATGATCTTCCGGTGAACTGCCGCCATTACATGGAGCGTATT
GCACAGCTAACTGGTGTGCAGGTTTCTATGTTCTCTGTAGGCCCTGATCGCGCTCAAACACATGTCATTAAAAGCGTTTG
GCGTTTAGCTTAA

Upstream 100 bases:

>100_bases
CATTGACTATTTTGGTGGCAAACGGTACAATGTATAGTGGAAAATGAAGAGGCTATGAAAAACGCATAGCTATCAAAGAC
CAATTAAATGAGGTGTTTAA

Downstream 100 bases:

>100_bases
TAAAATAAATATCAAGCCCATCTAAGGTGTATTCTTGGATGGGCTTTTTTGCTGTATAAAAATAATAGTGATTCTGGAAA
AGTATACAATTTTTTATCCG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGVTYKLHLIPSGIFYKEKISVIGNGMVVDPKALV
EELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERKGANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKL
EHNLGEKNRLLERFYELEGFKLEDILEEYYDYGQQFKEYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDTIGDTIREVGHEYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASLKDLARCEPVYEELPGWTEDITGVTSLDDLPVNCRHYMERI
AQLTGVQVSMFSVGPDRAQTHVIKSVWRLA

Sequences:

>Translated_430_residues
MSSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGVTYKLHLIPSGIFYKEKISVIGNGMVVDPKALV
EELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERKGANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKL
EHNLGEKNRLLERFYELEGFKLEDILEEYYDYGQQFKEYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDTIGDTIREVGHEYGTTTGRPRRVGWFDSVVVRHARRV
SGLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASLKDLARCEPVYEELPGWTEDITGVTSLDDLPVNCRHYMERI
AQLTGVQVSMFSVGPDRAQTHVIKSVWRLA
>Mature_429_residues
SSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGVTYKLHLIPSGIFYKEKISVIGNGMVVDPKALVE
ELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERKGANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKLE
HNLGEKNRLLERFYELEGFKLEDILEEYYDYGQQFKEYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSSN
PIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDTIGDTIREVGHEYGTTTGRPRRVGWFDSVVVRHARRVS
GLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASLKDLARCEPVYEELPGWTEDITGVTSLDDLPVNCRHYMERIA
QLTGVQVSMFSVGPDRAQTHVIKSVWRLA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=420, Percent_Identity=45.2380952380952, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI34577063, Length=422, Percent_Identity=42.8909952606635, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI40316944, Length=391, Percent_Identity=43.9897698209719, Blast_Score=300, Evalue=1e-81,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=48.7058823529412, Blast_Score=418, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=42.7230046948357, Blast_Score=325, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=42.7230046948357, Blast_Score=324, Evalue=5e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=424, Percent_Identity=42.2169811320755, Blast_Score=336, Evalue=3e-93,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=43.4052757793765, Blast_Score=332, Evalue=3e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47620; Mature: 47489

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGVTYKLHLIPSGIF
CCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCC
YKEKISVIGNGMVVDPKALVEELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERK
HHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEECCCCHHHC
GANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKLEHNLGEKNRLLERFYELEGF
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
KLEDILEEYYDYGQQFKEYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCEEECC
NPIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDTIGDTIREVGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASL
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHCCHHH
KDLARCEPVYEELPGWTEDITGVTSLDDLPVNCRHYMERIAQLTGVQVSMFSVGPDRAQT
HHHHHCCHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHH
HVIKSVWRLA
HHHHHHHHCC
>Mature Secondary Structure 
SSVVVVGTQWGDEGKGKITDFLSENAEAIARYQGGNNAGHTIKFDGVTYKLHLIPSGIF
CCEEEEEECCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCC
YKEKISVIGNGMVVDPKALVEELKYLHDKGVDTSNLRISNRAHIILPYHIRIDEADEERK
HHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEECCCCHHHC
GANKIGTTKKGIGPAYMDKAARVGIRIIDLLDKETFKEKLEHNLGEKNRLLERFYELEGF
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
KLEDILEEYYDYGQQFKEYVCDTSVVLNDALDDGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCEEECC
NPIAGGVTIGSGVGPSKINHVVGVAKAYTTRVGDGPFPTELFDTIGDTIREVGHEYGTTT
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRPRRVGWFDSVVVRHARRVSGLTDLSLTLLDVLTGIETLKICVAYKLDGKTITEFPASL
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHCCHHH
KDLARCEPVYEELPGWTEDITGVTSLDDLPVNCRHYMERIAQLTGVQVSMFSVGPDRAQT
HHHHHCCHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHH
HVIKSVWRLA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]