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The map label for this gene is ascB [H]

Identifier: 226222658

GI number: 226222658

Start: 23732

End: 25165

Strand: Direct

Name: ascB [H]

Synonym: Lm4b_00021

Alternate gene names: 226222658

Gene position: 23732-25165 (Clockwise)

Preceding gene: 226222656

Following gene: 226222660

Centisome position: 0.81

GC content: 39.68

Gene sequence:

>1434_bases
ATGACAGAATCAAAATTTCCTAAAGACTTTTTATGGGGCGGAGCAGTTGCTGCCAACCAATGTGAAGGAGCTTATCTTGA
AGACGGTAAAGGACTTTCACTTGTTGATATCCTTCCAACAGTAGAGGACGGACGTTGGGAAGCTTTATTCAATCCAGCGA
AAGCGCTTGCTACAGATTATGGTTTTTACCCAAGTCACGAATCAATTGATTTTTATCATCGTTATAAAGAAGACATTAAA
TTAATGGCAGAAATGGGATTCAAATGTTTCCGTATGTCCATCAGTTGGCCACGTATTTTCCCGAATGGTGATGAAACGAC
ACCAAATGAAAAAGGTTTAGCATTTTATGATGCAGTTTTTGATGAATGTCACAAATATGGTATTGAACCAGTTGTTACAA
TCAACCATTTTGATACTCCGCTAGAAGTTTTCAAAAAATATGGTGGTTGGAAAAATCGTAAATGCATCGACTTTTACTTG
AATTTCTGTGAAGCAATTTTCACGCGTTATAAAGATAAAGTAAAATATTGGATGACATTTAATGAAATTAACATGATTCT
TCATCTTCCATACATTGGTGGAGGTTTAGACGTTACTAAAGAGGACAATCCAGAGGAAGTTAAATATCAAGCTGCTCATC
ATCAATTAGTTGCATCAGCACTTGCAACTAAACTTGGTCATGAAATCAATCCTGAAAATCAAATCGGTTGTATGCTTGCA
GCGGGTAACACATATCCAATGACTTGTAATCCAAAAGATGTCTGGAAGTCTATCGAGGCGGACCGTGAAGGCTACTTCTT
CATTGATGTTCAAGCACGCGGATACTACCCAAGCTACACTAAGCGTTTCTTCAAAGAACACAACATCAACATCAAAATGG
AAGATGGCGACTTAGACGCATTACGCGATCACACAGTTGATTACGTGGCATTCAGCTACTATTCTTCTCGTCTAACAAGC
GCAGATCCAGAGAAAAACAAAGAAACAGAAGGCAACGTTTTCGCAACACTAAAAAACCCATACCTAAAAGCAAGCGAATG
GGGTTGGCAAATTGATCCACTAGGTCTACGTATTACTATGAATACAATTTACGACCGTTATCAAAAACCTCTTTTCATCG
TTGAAAATGGCTTAGGTGCAGTGGATACTGTGGCAGAAGACGGCTCAATCACTGATGATTACAGAATCGACTACATGCGT
GAACACGTTCGCGAAATGGGCGAAGCAATTGAGGATGGCGTGGAACTTCTTGGTTATACACCATGGGGCTGCATCGACCT
TGTCAGCGCTGGCTCTGGCGAAATGAAAAAACGCTACGGCTTCATCTACGTTGACCGCGATAATAAAGGTAACGGAACCC
TAAACCGCTCGAAGAAAAAATCATTCGACTGGTACAAAAAAGTAATTGAAACAAATGGTAAAGATATCGACTAA

Upstream 100 bases:

>100_bases
AAACGAATTTAAAAAATTTGAAATTATTGCTTGCTATGTAATGCATTACATATTCTATAGTGGAAAAGTAAATTATATTC
GTGAGAGGAGATATACAAAA

Downstream 100 bases:

>100_bases
TATATCAGAAATCCCCACTTTCATCATCGAAAGTGGGGATTTTATTTTAATATGAAATCGAGCGTTTTGCTTGTTTGCTC
CAACAAGTATTAAAATAACT

Product: beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTESKFPKDFLWGGAVAANQCEGAYLEDGKGLSLVDILPTVEDGRWEALFNPAKALATDYGFYPSHESIDFYHRYKEDIK
LMAEMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVFDECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYL
NFCEAIFTRYKDKVKYWMTFNEINMILHLPYIGGGLDVTKEDNPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLA
AGNTYPMTCNPKDVWKSIEADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDALRDHTVDYVAFSYYSSRLTS
ADPEKNKETEGNVFATLKNPYLKASEWGWQIDPLGLRITMNTIYDRYQKPLFIVENGLGAVDTVAEDGSITDDYRIDYMR
EHVREMGEAIEDGVELLGYTPWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID

Sequences:

>Translated_477_residues
MTESKFPKDFLWGGAVAANQCEGAYLEDGKGLSLVDILPTVEDGRWEALFNPAKALATDYGFYPSHESIDFYHRYKEDIK
LMAEMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVFDECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYL
NFCEAIFTRYKDKVKYWMTFNEINMILHLPYIGGGLDVTKEDNPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLA
AGNTYPMTCNPKDVWKSIEADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDALRDHTVDYVAFSYYSSRLTS
ADPEKNKETEGNVFATLKNPYLKASEWGWQIDPLGLRITMNTIYDRYQKPLFIVENGLGAVDTVAEDGSITDDYRIDYMR
EHVREMGEAIEDGVELLGYTPWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID
>Mature_476_residues
TESKFPKDFLWGGAVAANQCEGAYLEDGKGLSLVDILPTVEDGRWEALFNPAKALATDYGFYPSHESIDFYHRYKEDIKL
MAEMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVFDECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYLN
FCEAIFTRYKDKVKYWMTFNEINMILHLPYIGGGLDVTKEDNPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLAA
GNTYPMTCNPKDVWKSIEADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDALRDHTVDYVAFSYYSSRLTSA
DPEKNKETEGNVFATLKNPYLKASEWGWQIDPLGLRITMNTIYDRYQKPLFIVENGLGAVDTVAEDGSITDDYRIDYMRE
HVREMGEAIEDGVELLGYTPWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=496, Percent_Identity=27.0161290322581, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI110681710, Length=500, Percent_Identity=28.4, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI13273313, Length=490, Percent_Identity=26.734693877551, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI28376633, Length=486, Percent_Identity=27.9835390946502, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI24497614, Length=493, Percent_Identity=28.1947261663286, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=60.337552742616, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI2367270, Length=471, Percent_Identity=54.5647558386412, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI2367174, Length=477, Percent_Identity=55.3459119496855, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17552856, Length=494, Percent_Identity=28.7449392712551, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17539390, Length=497, Percent_Identity=28.3702213279678, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21356577, Length=498, Percent_Identity=25.5020080321285, Blast_Score=148, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54826; Mature: 54695

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESKFPKDFLWGGAVAANQCEGAYLEDGKGLSLVDILPTVEDGRWEALFNPAKALATDY
CCCCCCCHHHHCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHCHHHHHHHHC
GFYPSHESIDFYHRYKEDIKLMAEMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVF
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHH
DECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYLNFCEAIFTRYKDKVKYWMTF
HHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEE
NEINMILHLPYIGGGLDVTKEDNPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLA
EEEEEEEECCEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
AGNTYPMTCNPKDVWKSIEADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDA
ECCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCHH
LRDHTVDYVAFSYYSSRLTSADPEKNKETEGNVFATLKNPYLKASEWGWQIDPLGLRITM
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCEECCCEEEEEH
NTIYDRYQKPLFIVENGLGAVDTVAEDGSITDDYRIDYMREHVREMGEAIEDGVELLGYT
HHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID
CCCHHHHHCCCCCCHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TESKFPKDFLWGGAVAANQCEGAYLEDGKGLSLVDILPTVEDGRWEALFNPAKALATDY
CCCCCCHHHHCCCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHCHHHHHHHHC
GFYPSHESIDFYHRYKEDIKLMAEMGFKCFRMSISWPRIFPNGDETTPNEKGLAFYDAVF
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHH
DECHKYGIEPVVTINHFDTPLEVFKKYGGWKNRKCIDFYLNFCEAIFTRYKDKVKYWMTF
HHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEE
NEINMILHLPYIGGGLDVTKEDNPEEVKYQAAHHQLVASALATKLGHEINPENQIGCMLA
EEEEEEEECCEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
AGNTYPMTCNPKDVWKSIEADREGYFFIDVQARGYYPSYTKRFFKEHNINIKMEDGDLDA
ECCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCHH
LRDHTVDYVAFSYYSSRLTSADPEKNKETEGNVFATLKNPYLKASEWGWQIDPLGLRITM
HHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCEECCCEEEEEH
NTIYDRYQKPLFIVENGLGAVDTVAEDGSITDDYRIDYMREHVREMGEAIEDGVELLGYT
HHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PWGCIDLVSAGSGEMKKRYGFIYVDRDNKGNGTLNRSKKKSFDWYKKVIETNGKDID
CCCHHHHHCCCCCCHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]