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The map label for this gene is cmtB [H]

Identifier: 225861950

GI number: 225861950

Start: 1992280

End: 1994310

Strand: Reverse

Name: cmtB [H]

Synonym: SPT_2143

Alternate gene names: 225861950

Gene position: 1994310-1992280 (Counterclockwise)

Preceding gene: 225861955

Following gene: 225861949

Centisome position: 94.42

GC content: 32.94

Gene sequence:

>2031_bases
ATGTTTGATTATAGAGCACTAGTTATTTTGATGACTTTGATGGATCATTGTGAGCTATCATTATATGAATTATCTGTTAA
GGTGAGCTTACCTATAAAGGAAGTCAAAGAAGGAATAGATTATTTAGTGCCTTATCTAGCTAATAAAGGGATAGTGCTGG
ATAAAAAACAAGGTCGCTATAGTTTATCAAATCGTACGAAGCAGTCTTTGACAGATATTATTAAGTCGGATGAATTGGTT
TTACCAAAGTCGACTCGCTTAGCTTTAATCTATCTTTACACTTTTTGCCGATTAGATTTTATATCGAATAATCATTACCA
AGACTTTTTGAAAGTAAGTAAGAATACAACCTTATCTGATATTCAATCATTAAGAAAGATTATGTTAGATAATGATTTGG
AGCTAGGGTACAGTAGAGCAAAAGGTTATACTTTACACGGTTCAGAGTGGAATAAGCACCGTTTAGCTTTTCAAATGGTT
AGTGAGTTGCTGGAATCCTCCATAGGGATTTGGGGTTTGGATTATGTTTTATCCAGTTGGGGATATTCATTAACTTATGA
TTTGATTGATCAGGTAGTTAAAGATTATTATGAGAAGCTACAGCTAGTACCTATTGTTAATCAATTAAAAGTTTACCTTT
TCGGTTTAGTATTCATTCTGTGTAGGTATCAAAGGGATGTTGAAAGAGTATGTCTTTCAGAGACATTAGTATCTCCTGTT
ATTCAAGATATAACGACTATTTTATTGGATACAGTGGTTGATTTGGGAATTATAGATACGGTGTTTTCTGAGGATGATTA
TCGCTATATCACAGTTTTATTATCAAGTTGCTTTGAAGGTGAAGTAGATGTTGCTCCGGTTTACTTTAATCAATTAACAG
AGGCTATTATAAGTAGAATGGAAGATATTTCTTTGTTACATTTTAAACAAAGGGAAGGATTGAGAGAAAATCTTCGTCGC
CACCTTATACCGGCTTACTATAGATTAAAGTTCGGCCTACCTAGTTCAAATGAATATGTATTGCATGTTAAAGAACATTA
TCCTGATTTATTTGAATTAGTCAAAGATTCTTTGATGCCCTTGATGGACGCTATTGACAATCCCATACCTGATAGTGAAA
CAGCATATTTTGTTATCCATTTCGGAGGTTATTTAAAGAAAGCAGATACTTTGCCTCAAAAATGGTATAAAGCAGCAATC
ATTTGTCCTAATGGTGTTAGTTCTTCATTGATGTTAAAAGAGAATCTATTAGCATTATTTCCTCAAATTGAGTTTATAGG
AACCTCAAAGATTGATGATTTACAGGTGAAAGCTAGTAGTGACTATGATATGGTTTTTTCTACCATAAAGGTGGAGACAG
AAAAGCCAAATTATCTAGTTTCGGTTATGATGACTGAAGAGCAAGCAATACAACTAGTAGAACTAGTGTTAAAAGATTTT
CCGAATTTAGAGTATGGAGATTTTGAGATTGAACAAATCCTGAATATTGTCAAAAGATATGGAATTATTACACAAGAATT
GGAATTAAGATTGGCGTTAAAGAATTATCTTTATCAAAAAAATGATAGAAAGGAAATTGTACCAGTGCTAGAACAACTTA
TTACCAAAGAAACCTATCAGGTTAGTTCGCAAAAATTAGGATGGAAGGAGGCGATTCGTTTAGCAGCTAAACCGCTTTTG
GATCAAGATAAGATAACGGAGAACTACCCTGAGGCAATGATTCAAAAAGTAGAAGAGTTTGGACCTTTTATTAATTTAGG
GAAGGGAGTAGCAATTCCTCACGCTCGGCCAGATGAAGGTGTGAATGAAATAGGAATGTCAATGTTAGTTTTGGAAGAAC
CGATTTATTTATTGGATAATCCAGAACAAGAGGTAAGATTGTTGATTTGTATTGCAGCCATTGATAATGAGAGTCATTTA
AAGGCTTTGTCACATTTAACAACAATTTTAAGAGATAAAAATCATGTTCAAACTTTAATTTCATCAAAAAACTATGATGA
CATTAAAATGATAATTAAACAGGAGGATTAG

Upstream 100 bases:

>100_bases
AGCCCAATAATTTCTTTTAAAAGGTGAGATTATATTGTGCTCAAATACTCTTTTATTCTTATAATTTTTACACTATAATT
GTAGTTAGAAAGGAAGAGAA

Downstream 100 bases:

>100_bases
ATAATGTTAAAAATTGGTACAGCTTGTGGTTCAGGATTAGGTTCAAGTTTTATGGTACAGATGAATATTGAATCTGTATT
GAGTGATTTGAATGTTTCGG

Product: transcriptional regulator, BglG family protein

Products: Protein Histidine; Sugar Phosphate. [C]

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVPYLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELV
LPKSTRLALIYLYTFCRLDFISNNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVFILCRYQRDVERVCLSETLVSPV
IQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSSCFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRR
HLIPAYYRLKFGLPSSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLPQKWYKAAI
ICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIKVETEKPNYLVSVMMTEEQAIQLVELVLKDF
PNLEYGDFEIEQILNIVKRYGIITQELELRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLL
DQDKITENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQEVRLLICIAAIDNESHL
KALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED

Sequences:

>Translated_676_residues
MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVPYLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELV
LPKSTRLALIYLYTFCRLDFISNNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVFILCRYQRDVERVCLSETLVSPV
IQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSSCFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRR
HLIPAYYRLKFGLPSSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLPQKWYKAAI
ICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIKVETEKPNYLVSVMMTEEQAIQLVELVLKDF
PNLEYGDFEIEQILNIVKRYGIITQELELRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLL
DQDKITENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQEVRLLICIAAIDNESHL
KALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED
>Mature_676_residues
MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVPYLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELV
LPKSTRLALIYLYTFCRLDFISNNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVFILCRYQRDVERVCLSETLVSPV
IQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSSCFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRR
HLIPAYYRLKFGLPSSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLPQKWYKAAI
ICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIKVETEKPNYLVSVMMTEEQAIQLVELVLKDF
PNLEYGDFEIEQILNIVKRYGIITQELELRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLL
DQDKITENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQEVRLLICIAAIDNESHL
KALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3711

COG function: function code K; Transcriptional antiterminator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789302, Length=96, Percent_Identity=40.625, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI2367359, Length=147, Percent_Identity=24.4897959183673, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790755, Length=128, Percent_Identity=25.78125, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 77946; Mature: 77946

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVPYLANKGIVLDKKQGRY
CCCHHHHHHHHHHHHHCCEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCC
SLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFISNNHYQDFLKVSKNTTLSD
CHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHH
IQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMVSELLESSIGIWGLDYVLSSW
HHHHHHHHCCCCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
GYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVFILCRYQRDVERVCLSETLVSPV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSSCFEGEVDVAPVYFNQLTEAIISRM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EDISLLHFKQREGLRENLRRHLIPAYYRLKFGLPSSNEYVLHVKEHYPDLFELVKDSLMP
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCEEEEEHHHCCHHHHHHHHHHHH
LMDAIDNPIPDSETAYFVIHFGGYLKKADTLPQKWYKAAIICPNGVSSSLMLKENLLALF
HHHHHCCCCCCCCCEEEEEEECCHHHHHHCCHHHHHCEEEECCCCCCCHHHHHHHHHHHC
PQIEFIGTSKIDDLQVKASSDYDMVFSTIKVETEKPNYLVSVMMTEEQAIQLVELVLKDF
CCCEEECCCCCCCEEEECCCCHHHEEEEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHC
PNLEYGDFEIEQILNIVKRYGIITQELELRLALKNYLYQKNDRKEIVPVLEQLITKETYQ
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VSSQKLGWKEAIRLAAKPLLDQDKITENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEG
HHHHCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
VNEIGMSMLVLEEPIYLLDNPEQEVRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLI
HHHHCCHHHHHHCCEEEECCCHHHEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
SSKNYDDIKMIIKQED
CCCCCCCEEEEEECCC
>Mature Secondary Structure
MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVPYLANKGIVLDKKQGRY
CCCHHHHHHHHHHHHHCCEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEEECCCCCC
SLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFISNNHYQDFLKVSKNTTLSD
CHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHH
IQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMVSELLESSIGIWGLDYVLSSW
HHHHHHHHCCCCCCCCHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
GYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVFILCRYQRDVERVCLSETLVSPV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSSCFEGEVDVAPVYFNQLTEAIISRM
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
EDISLLHFKQREGLRENLRRHLIPAYYRLKFGLPSSNEYVLHVKEHYPDLFELVKDSLMP
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCEEEEEHHHCCHHHHHHHHHHHH
LMDAIDNPIPDSETAYFVIHFGGYLKKADTLPQKWYKAAIICPNGVSSSLMLKENLLALF
HHHHHCCCCCCCCCEEEEEEECCHHHHHHCCHHHHHCEEEECCCCCCCHHHHHHHHHHHC
PQIEFIGTSKIDDLQVKASSDYDMVFSTIKVETEKPNYLVSVMMTEEQAIQLVELVLKDF
CCCEEECCCCCCCEEEECCCCHHHEEEEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHC
PNLEYGDFEIEQILNIVKRYGIITQELELRLALKNYLYQKNDRKEIVPVLEQLITKETYQ
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VSSQKLGWKEAIRLAAKPLLDQDKITENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEG
HHHHCCCHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
VNEIGMSMLVLEEPIYLLDNPEQEVRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLI
HHHHCCHHHHHHCCEEEECCCHHHEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHH
SSKNYDDIKMIIKQED
CCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]