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The map label for this gene is glnM [H]

Identifier: 225858443

GI number: 225858443

Start: 600280

End: 600957

Strand: Reverse

Name: glnM [H]

Synonym: SP70585_0670

Alternate gene names: 225858443

Gene position: 600957-600280 (Counterclockwise)

Preceding gene: 225858444

Following gene: 225858442

Centisome position: 27.51

GC content: 43.22

Gene sequence:

>678_bases
ATGACAGATTTATCATCTTGGACAGCCTATTTTCAGGATTTTGAACAATTTTTCAATGGTTTCCTCTTTACTCTTGCCCT
AGCGATTGGATCCTTTATCCTCGCCATGGTCTTAGGAATCTTCTTTGGTGCCATGTCAACCAGTAAACGTCCAATTTTAC
GCATTTTAGCTCGCATCTTTGTTGAATTTTACCAAAATACTCCTCTCTTGGTGCAGTTTGTTATTGTTTTTTATGGCCTA
CCTCTTATCAGTGACCATATCATCATGATTCCAATTTATTGGACAGCCGTTCTCTGCGTGGGACTCTATCACGGCGCTTA
TATCGCTGAGGTTATTCGTTCAGGGATTCAGTCTATTCCTAGTGGTCAGATGGAGGCCGCCTTGTCGCAAGGTTTTACCT
ATATCAGTGCCATGCGCTTGATTACCTTGCCTCAGGCCTTCCGCATCATTCTCCCTCCATTGACCAACCAAATTGTTAAC
CTCATCAAGAACACATCTACTGTAGCTATCATCTCTGGAGTAGACTTGATGTTTGTGACTAAGTCTTGGTCGGCTCTCAA
CGGAAACTATATCCCAGCCTTTTTAGGTGCTGCTCTTCTCTACTTTGCCCTATGCTTCCCTGTTGCCCAGTTTGGTCGCA
AGATGGAGCAAGCCAATAAAAAAGCCTATTCACTTTAG

Upstream 100 bases:

>100_bases
CTTGGTTACTAAATGGAGCAAGGATGGTAGTTTGCAGAAACTTTATGACCGTTACAAGCTCAAACCATCTAGTCATACCG
CAGATTAAGGAGGATACCCT

Downstream 100 bases:

>100_bases
GAGGTTACTATGGAATCTATTTTAGAAATTTTAACCCCAGATAACCTAGTCTTTATCTTTAAAGGATTTGGCTTGACCCT
CTATATTTCTCTGATTGCCA

Product: ABC transporter membrane-spanning permease - glutamine transport

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTDLSSWTAYFQDFEQFFNGFLFTLALAIGSFILAMVLGIFFGAMSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGL
PLISDHIIMIPIYWTAVLCVGLYHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQANKKAYSL

Sequences:

>Translated_225_residues
MTDLSSWTAYFQDFEQFFNGFLFTLALAIGSFILAMVLGIFFGAMSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGL
PLISDHIIMIPIYWTAVLCVGLYHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQANKKAYSL
>Mature_224_residues
TDLSSWTAYFQDFEQFFNGFLFTLALAIGSFILAMVLGIFFGAMSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLP
LISDHIIMIPIYWTAVLCVGLYHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVNL
IKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQANKKAYSL

Specific function: Part of the ABC transporter complex glnHMPQ involved in glutamine transport. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=169, Percent_Identity=37.2781065088757, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082239, Length=208, Percent_Identity=34.6153846153846, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788226, Length=206, Percent_Identity=33.0097087378641, Blast_Score=107, Evalue=4e-25,
Organism=Escherichia coli, GI1787030, Length=215, Percent_Identity=27.906976744186, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1786873, Length=211, Percent_Identity=27.4881516587678, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994930, Length=91, Percent_Identity=42.8571428571429, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788645, Length=207, Percent_Identity=26.5700483091787, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787086, Length=175, Percent_Identity=28, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788646, Length=172, Percent_Identity=27.3255813953488, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787087, Length=181, Percent_Identity=26.5193370165746, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25086; Mature: 24955

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLSSWTAYFQDFEQFFNGFLFTLALAIGSFILAMVLGIFFGAMSTSKRPILRILARIF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGLYHGAYIAEVIRSGIQSIP
HHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVNLIKNTSTVAIISGVDLMFVT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHEEEE
KSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQANKKAYSL
CCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDLSSWTAYFQDFEQFFNGFLFTLALAIGSFILAMVLGIFFGAMSTSKRPILRILARIF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGLYHGAYIAEVIRSGIQSIP
HHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVNLIKNTSTVAIISGVDLMFVT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHEEEE
KSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQANKKAYSL
CCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 9384377 [H]