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The map label for this gene is gatA

Identifier: 225858288

GI number: 225858288

Start: 453319

End: 454785

Strand: Reverse

Name: gatA

Synonym: SP70585_0506

Alternate gene names: 225858288

Gene position: 454785-453319 (Counterclockwise)

Preceding gene: 225858289

Following gene: 225858287

Centisome position: 20.82

GC content: 44.99

Gene sequence:

>1467_bases
ATGACTTTTAACAATAAAACTATTGAAGAGTTGCACAATCTCCTTGTCTCTAAGGAAATTTCTGCAACAGAATTGACCCA
AGCAACACTTGAAAATATCAAGTCTCGTGAGGAAGCCCTCAATTCATTTGTCACCATCGCTGAGGAGCAAGCTCTTGTTC
AAGCTAAAGCCATTGATGAAGCTGGAATTGATGCTAACAATGTCCTTTCAGGAATTCCACTTGCTGTTAAGGATAACATC
TCTACAGACGGTATTCTCACAACTGCTGCCTCAAAAATGCTCTACAACTATGAGCCAATCTTTGATGCGACAGCTGTTGC
CAATGCAAAAACCAAAGGCATGATTGTCGTTGGAAAGACCAACATGGACGAATTTGCTATGGGTGGTTCCGGTGAAACTT
CACACTACGGAGCAACTAAAAACGCTTGGGACCACAGCAAGGTTCCTGGTGGGTCATCAAGTGGTTCTGCCGCAGCTGTA
GCCTCAGGACAAGTTCGCTTGTCACTTGGTTCTGATACTGGTGGTTCCATCCGCCAACCTGCTGCCTTCAACGGAATCGT
TGGTCTCAAACCAACCTACGGAACAGTTTCACGTTTCGGTCTCATTGCCTTTGGTAGCTCATTAGACCAGATTGGACCTT
TTGCTCCTACTGTTAAGGAAAATGCCCTCTTGCTCAACGCTATTGCCAGCGAAGATACTAAAGACTCTACTTCTGCTCCT
GTCCGCATCGCCGACTTTACTTCAAAAATCGGCCAAGACATTAAGGGCATGAAAATTGCTTTGCCTAAGGAATACCTAGG
CGAAGGAATTGATCCAGAGGTTAAGGAAACAATCTTAAACGCGGCCAAACACTTTGAAAAATTGGGTGCTATCGTCGAAG
AAGTCAGCCTTCCTCACTCTAAATACGGTGTTGCCGTTTATTACATCATCGCTTCATCAGAAGCTTCATCAAACTTGCAA
CGTTTTGACGGTATCCGTTACGGCTACCGCGCAGAAGATGCGACTAATCTTGATGAAATCTATGTAAACAGCCGTAGCCA
AGGTTTTGGTGAAGAAGTGAAGCGCCGTATCATGCTGGGTACTTTCAGTCTTTCATCAGGTTACTACGATGCCTACTACA
AGAAGGCTGGACAGGTCCGTACACTTATCATTCAAGATTTCGAAAAAGTCTTCGCGGATTACGATTTGATTTTGGGTCCA
ACTGCTCCAAGTGTTGCCTATGACTTGGATTCTCTCAACCATGACCCAGTTGCCATGTACTTAGCCGACCTCTTGACCAT
ACCTGTAAACTTGGCAGGACTGCCTGGAATTTCGATTCCTGCTGGATTCTCTCAAGGTCTACCTGTCGGACTCCAATTGA
TTGGTCCCAAGTACTCTGAGGAAACCATTTACCAAGCTGCTGCTGCTTTTGAAGCAACAACAGACTACCACAAACAACAA
CCCGTGATTTTTGGAGGTGACAACTAA

Upstream 100 bases:

>100_bases
AAGAAGGAACAGACCGTGATCGCTTGTTTAAAAACGTACCTGAACAAGACAACTACTATATCAAGGTGCCAGCTATCCTA
GACGATGGAGGAGATGCCTA

Downstream 100 bases:

>100_bases
TGAACTTTGAAACAGTCATCGGACTTGAAGTCCACGTAGAGCTCAACACCAATTCAAAAATCTTCTCACCTACTTCTGCC
CACTTTGGAAATGACCAAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNI
STDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDTKDSTSAP
VRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGP
TAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKYSEETIYQAAAAFEATTDYHKQQ
PVIFGGDN

Sequences:

>Translated_488_residues
MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNI
STDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDTKDSTSAP
VRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQ
RFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGP
TAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKYSEETIYQAAAAFEATTDYHKQQ
PVIFGGDN
>Mature_487_residues
TFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNIS
TDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAVA
SGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDTKDSTSAPV
RIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQR
FDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGPT
APSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKYSEETIYQAAAAFEATTDYHKQQP
VIFGGDN

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=38.1422924901186, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=25.1533742331288, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17543272, Length=452, Percent_Identity=36.0619469026549, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17537465, Length=479, Percent_Identity=24.4258872651357, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=182, Percent_Identity=32.4175824175824, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=321, Percent_Identity=24.9221183800623, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=188, Percent_Identity=32.4468085106383, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=151, Percent_Identity=34.4370860927152, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=151, Percent_Identity=34.4370860927152, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=450, Percent_Identity=33.3333333333333, Blast_Score=230, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=436, Percent_Identity=30.9633027522936, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320448, Length=195, Percent_Identity=29.7435897435897, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=41.0204081632653, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24652985, Length=480, Percent_Identity=26.25, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19922090, Length=480, Percent_Identity=26.25, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24652981, Length=480, Percent_Identity=26.25, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24652983, Length=480, Percent_Identity=26.25, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=23.8747553816047, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078093, Length=503, Percent_Identity=24.6520874751491, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45550774, Length=197, Percent_Identity=34.5177664974619, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648435, Length=197, Percent_Identity=34.5177664974619, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648437, Length=197, Percent_Identity=34.5177664974619, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=23.015873015873, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648441, Length=129, Percent_Identity=40.3100775193798, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648439, Length=129, Percent_Identity=40.3100775193798, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52090; Mature: 51959

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDE
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKT
CCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECC
NMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAVASGQVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDTKDSTSAP
CCCCCCEECCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEEEHHCCCCCCCCCCC
VRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCHHHHHHHHEEE
TFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGPTAPSVAYDLDSLNHDPVAMY
EEECCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKYSEETIYQAAAAFEATTDYHKQQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
PVIFGGDN
CEEECCCC
>Mature Secondary Structure 
TFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFVTIAEEQALVQAKAIDE
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFDATAVANAKTKGMIVVGKT
CCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECC
NMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAVASGQVRLSLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCC
AAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTVKENALLLNAIASEDTKDSTSAP
CCCCCCEECCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEEEEHHCCCCCCCCCCC
VRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKETILNAAKHFEKLGAIVEEVSLPHS
EEEHHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDATNLDEIYVNSRSQGFGEEVKRRIMLG
CCCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCHHHHHHHHEEE
TFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFADYDLILGPTAPSVAYDLDSLNHDPVAMY
EEECCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCCEEECCCCCCHHHHHHCCCCCHHHHH
LADLLTIPVNLAGLPGISIPAGFSQGLPVGLQLIGPKYSEETIYQAAAAFEATTDYHKQQ
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
PVIFGGDN
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA