Click here to switch to the map view.

The map label for this gene is gatB

Identifier: 225858287

GI number: 225858287

Start: 451877

End: 453319

Strand: Reverse

Name: gatB

Synonym: SP70585_0505

Alternate gene names: 225858287

Gene position: 453319-451877 (Counterclockwise)

Preceding gene: 225858288

Following gene: 225858286

Centisome position: 20.75

GC content: 45.81

Gene sequence:

>1443_bases
ATGAACTTTGAAACAGTCATCGGACTTGAAGTCCACGTAGAGCTCAACACCAATTCAAAAATCTTCTCACCTACTTCTGC
CCACTTTGGAAATGACCAAAATGCCAACACTAACGTGATTGACTGGTCTTTCCCAGGAGTTCTACCAGTTCTCAATAAAG
GGGTTGTTGATGCCGGTATCAAGGCTGCTCTTGCCCTCAACATGGACCTCCACAAAAAAATGCACTTTGACCGCAAGAAC
TACTTCTACCCTGATAATCCCAAAGCCTACCAAATTTCTCAGTTTGATGAGCCAATCGGTTATAACGGCTGGATTGAAAT
CGAGCTAGAAGACGGTACGACCAAGAAAATTGGTATCGAACGCGCCCACTTAGAGGAAGACGCTGGTAAAAACACCCACG
GTACAGACGGCTACTCTTACGTTGACCTTAACCGCCAAGGGGTACCTTTGATTGAGATTGTATCTGAAGCCGATATGCGT
TCGCCAGAGGAGGCCTATGCTTATCTGACAGCCCTCAAGGAAGTTATCCAGTACGCTGGCATTTCTGACGTTAAGATGGA
AGAAGGTTCTATGCGTGTGGATGCCAACATCTCCCTTCGCCCTTATGGTCAAGAAAAATTCGGTACCAAGACTGAATTGA
AGAACCTCAACTCCTTCTCAAACGTTCGTAAAGGTCTTGAATACGAAGTCCAACGCCAGGCTGAAATTCTTCGCTCAGGT
GGTCAAATCCGCCAAGAAACACGCCGTTACGATGAAGCTAACAAAACAACCATCCTCATGCGTGTCAAGGAAGGGGCTGC
TGACTACCGCTACTTCCCAGAACCAGACCTACCCCTCTTTGAAATTTCTGACGAGTGGATTGAGGAAATGCGGACTGAGT
TGCCAGAGTTTCCAAAAGAACGTCGTGCGCGTTATGTATCTGACCTTGGTTTATCAGACTACGATGCTAGTCAGTTGACT
GCTAATAAAGTCACTTCTGACTTCTTTGAAAAAGCTGTTGCCCTAGGTGGTGATGCCAAACAAGTCTCTAACTGGCTCCA
AGGGGAAGTCGCTCAGTTCTTGAATGCTGAAGGTAAAACACTGGAACAAATCGAATTGACACCAGAAAACTTGGTTGAAA
TGATTACCATCATCGAAGACGGTACTATTTCATCTAAGATTGCCAAGAAAGTCTTTGTCCATCTAGCTAAAAATGGCGGT
GGCGCGCGTGAATACGTGGAAAAAGCAGGTATGGTTCAAATTTCAGATCCAGCTATCTTGATCCCAATCATCCACCAAGT
CTTTGCCGATAACGAAGCTGCTGTTGCCGACTTCAAGTCAGGCAAACGTAACGCCGACAAGGCCTTTACAGGATTCCTTA
TGAAGGCAACCAAAGGCCAAGCCAACCCACAAGTTGCCCTTAAACTACTTGCACAGGAATTGGCGAAGTTGAAAGAAAAC
TAG

Upstream 100 bases:

>100_bases
CCAAGTACTCTGAGGAAACCATTTACCAAGCTGCTGCTGCTTTTGAAGCAACAACAGACTACCACAAACAACAACCCGTG
ATTTTTGGAGGTGACAACTA

Downstream 100 bases:

>100_bases
ACAGAACAAAACCAGCCCTAAGGTTGGTTTTTTCTTCTCTACCAACTCCCAATAACTATTTTGGCTTTATTTCCAGAGTA
TTTTATGGTAAAATGAAGAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIEIELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
GQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLT
ANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVFVHLAKNGG
GAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN

Sequences:

>Translated_480_residues
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIEIELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
GQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLT
ANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVFVHLAKNGG
GAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN
>Mature_480_residues
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGWIEIELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
GQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLT
ANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVFVHLAKNGG
GAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=34.3434343434343, Blast_Score=272, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI115534094, Length=304, Percent_Identity=39.1447368421053, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=502, Percent_Identity=29.2828685258964, Blast_Score=198, Evalue=2e-51,
Organism=Drosophila melanogaster, GI45550808, Length=496, Percent_Identity=32.258064516129, Blast_Score=249, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53733; Mature: 53733

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHHCC
KAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYNGWIEIELEDGTTKKIGIE
CEEEEECHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCH
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRSPEEAYAYLTALKEVIQYAG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
CCCEEECCCCEEEECCEEECCCCCHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
GQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKE
CHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RRARYVSDLGLSDYDASQLTANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
LEQIELTPENLVEMITIIEDGTISSKIAKKVFVHLAKNGGGAREYVEKAGMVQISDPAIL
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCEEH
IPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSFPGVLPVLNKGVVDAGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHHCC
KAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYNGWIEIELEDGTTKKIGIE
CEEEEECHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCH
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRSPEEAYAYLTALKEVIQYAG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNSFSNVRKGLEYEVQRQAEILRSG
CCCEEECCCCEEEECCEEECCCCCHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
GQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPDLPLFEISDEWIEEMRTELPEFPKE
CHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHH
RRARYVSDLGLSDYDASQLTANKVTSDFFEKAVALGGDAKQVSNWLQGEVAQFLNAEGKT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCE
LEQIELTPENLVEMITIIEDGTISSKIAKKVFVHLAKNGGGAREYVEKAGMVQISDPAIL
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCEEH
IPIIHQVFADNEAAVADFKSGKRNADKAFTGFLMKATKGQANPQVALKLLAQELAKLKEN
HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA