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The map label for this gene is insK [H]

Identifier: 225858248

GI number: 225858248

Start: 415823

End: 416575

Strand: Reverse

Name: insK [H]

Synonym: SP70585_0466

Alternate gene names: 225858248

Gene position: 416575-415823 (Counterclockwise)

Preceding gene: 225858255

Following gene: 225858247

Centisome position: 19.07

GC content: 33.73

Gene sequence:

>753_bases
ATGAACTACACCCCAAAAGTTAGACAGAAAAAATCTAACTTTTGGGGTGTTTTTATTATGAAATTAACTTATGATGATAA
AGTTCAGATCTATGAACTTAGAAAACAAGGATATAGCTTAGAGAAGCTTTCAAATAAATTTGGGATAAACAATTCTAATC
TTAGGTACATGATTAAATTGATTGATCGTTACGGAATAGAGTTTGTCAAAAAAGGAAAAAATCGTTACTATTCTCCTGAT
TTAAAACAAGAAATGATTAATAAAGTCTTATATGAAGGCTGGACTAAAGATAGAGTTTCTCTTGAATACGGCCTCCCAAG
TCGTACGATACTTCTTAACTGGCTAGCACAATACAGGAAAAACGGGTATACTATTGTTGAGAAAACAAGAGGGAAAGTAC
CTGAGAGCGGAGAATGCCATCCTAAAAAAGTTAAGAGAACTCCGATTGAAGGAGGAAAAAGAGAAAGAAGAAAGACAGAA
ATTGTTCAAGAATTAATGACTGAGTTTTCGTTAGATCTTCTTCTAAAAGCCATTAAACTAGCTCGTTCGACCTACTACTA
TCACTTGAAACAGCTAGATAAACCAGATAAGGACCAAGAGCTTAAAGCTGAAATTCAATCCATTTTTATCGAACACAAGG
GAAAGTATGCTCATCGCCGGGTTCATTTAGAACTAAGAAATCGTGGTTATCTGGTAAATCATAAAAGAGTTCAAGGCTTG
ATGAAAGTACTCAATTTACAAGATAAAATGTGA

Upstream 100 bases:

>100_bases
AAAACTAAAGTTTTGTCACAGATTTTGTGTCAAAACATTACTTTTGATGAGGCTGGGCAAAAAGGCTTAAAAATTATAAA
ACGCATCATATCAGGTGTAC

Downstream 100 bases:

>100_bases
CGGCAAGAAGGCAGAGAATCTCATTCAAGGCCAATTTGAAGGCTCTAAAACAATGGAAAAGTGCTACACAGATGTGACAG
AATTTGCCATTCCAGCAAGT

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNYTPKVRQKKSNFWGVFIMKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDRYGIEFVKKGKNRYYSPD
LKQEMINKVLYEGWTKDRVSLEYGLPSRTILLNWLAQYRKNGYTIVEKTRGKVPESGECHPKKVKRTPIEGGKRERRKTE
IVQELMTEFSLDLLLKAIKLARSTYYYHLKQLDKPDKDQELKAEIQSIFIEHKGKYAHRRVHLELRNRGYLVNHKRVQGL
MKVLNLQDKM

Sequences:

>Translated_250_residues
MNYTPKVRQKKSNFWGVFIMKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDRYGIEFVKKGKNRYYSPD
LKQEMINKVLYEGWTKDRVSLEYGLPSRTILLNWLAQYRKNGYTIVEKTRGKVPESGECHPKKVKRTPIEGGKRERRKTE
IVQELMTEFSLDLLLKAIKLARSTYYYHLKQLDKPDKDQELKAEIQSIFIEHKGKYAHRRVHLELRNRGYLVNHKRVQGL
MKVLNLQDKM
>Mature_250_residues
MNYTPKVRQKKSNFWGVFIMKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKLIDRYGIEFVKKGKNRYYSPD
LKQEMINKVLYEGWTKDRVSLEYGLPSRTILLNWLAQYRKNGYTIVEKTRGKVPESGECHPKKVKRTPIEGGKRERRKTE
IVQELMTEFSLDLLLKAIKLARSTYYYHLKQLDKPDKDQELKAEIQSIFIEHKGKYAHRRVHLELRNRGYLVNHKRVQGL
MKVLNLQDKM

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=87, Percent_Identity=47.1264367816092, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 29885; Mature: 29885

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTPKVRQKKSNFWGVFIMKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKL
CCCCCCHHHHCCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHH
IDRYGIEFVKKGKNRYYSPDLKQEMINKVLYEGWTKDRVSLEYGLPSRTILLNWLAQYRK
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHC
NGYTIVEKTRGKVPESGECHPKKVKRTPIEGGKRERRKTEIVQELMTEFSLDLLLKAIKL
CCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARSTYYYHLKQLDKPDKDQELKAEIQSIFIEHKGKYAHRRVHLELRNRGYLVNHKRVQGL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEECHHHHHHH
MKVLNLQDKM
HHHHHHHCCC
>Mature Secondary Structure
MNYTPKVRQKKSNFWGVFIMKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIKL
CCCCCCHHHHCCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHH
IDRYGIEFVKKGKNRYYSPDLKQEMINKVLYEGWTKDRVSLEYGLPSRTILLNWLAQYRK
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHC
NGYTIVEKTRGKVPESGECHPKKVKRTPIEGGKRERRKTEIVQELMTEFSLDLLLKAIKL
CCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARSTYYYHLKQLDKPDKDQELKAEIQSIFIEHKGKYAHRRVHLELRNRGYLVNHKRVQGL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHEEEEEECCCCEEECHHHHHHH
MKVLNLQDKM
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]