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The map label for this gene is insK [H]

Identifier: 225858247

GI number: 225858247

Start: 415282

End: 415770

Strand: Reverse

Name: insK [H]

Synonym: SP70585_0465

Alternate gene names: 225858247

Gene position: 415770-415282 (Counterclockwise)

Preceding gene: 225858248

Following gene: 225858234

Centisome position: 19.03

GC content: 38.24

Gene sequence:

>489_bases
ATGGAAAAGTGCTACACAGATGTGACAGAATTTGCCATTCCAGCAAGTACTCAAAAGCTTTACTTATCACCAGTTTTAGA
TGGCTTTAATAGCGAAATTATCGCCTATAATCTTTCAACTTCACCCAACTTAGAACAAGTACAAACAATGTTGGAACAGG
CATTCACAGAAAAGCACTATGAGAATACGATTCTCCATAGTGACCAAGGCTGGCAATACCGACACGATTCTTATCATCGG
TTCCTAGAGAGTAAGGGAATTCAAGCATCTATGTCACGCAAGGGAAACAGCCAAGACAACGGTAGGATGGAATCTTTCTT
TGGGATTCTGAAATCGGAAATGTTTTACGGTTATGAGAAGTCGTTTCAGTCGCTTAAGCAATTGGAACAAGCTATTGTAG
ACTATATTGATTACTACAACAACAAGAGAATTAAGGTAAAACTAAAAGGACTTAGTCCTGTGCAGTACAGAACTAAATCC
TTTGAATAA

Upstream 100 bases:

>100_bases
AGAGTTCAAGGCTTGATGAAAGTACTCAATTTACAAGATAAAATGTGACGGCAAGAAGGCAGAGAATCTCATTCAAGGCC
AATTTGAAGGCTCTAAAACA

Downstream 100 bases:

>100_bases
ATTATTTGTCTAACTTTTTGGGGGCAGTACATTGTTAACTTGATACTATGCGTTTTTTGATTGTGAAGACTTTTATCCAC
CTATTATACAAAGTCAAGTA

Product: transposase, IS3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAFTEKHYENTILHSDQGWQYRHDSYHR
FLESKGIQASMSRKGNSQDNGRMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKS
FE

Sequences:

>Translated_162_residues
MEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAFTEKHYENTILHSDQGWQYRHDSYHR
FLESKGIQASMSRKGNSQDNGRMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKS
FE
>Mature_162_residues
MEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAFTEKHYENTILHSDQGWQYRHDSYHR
FLESKGIQASMSRKGNSQDNGRMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKS
FE

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=161, Percent_Identity=52.1739130434783, Blast_Score=171, Evalue=2e-44,
Organism=Escherichia coli, GI1788405, Length=144, Percent_Identity=32.6388888888889, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1787263, Length=144, Percent_Identity=32.6388888888889, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1786753, Length=144, Percent_Identity=32.6388888888889, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1786570, Length=144, Percent_Identity=32.6388888888889, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1786490, Length=144, Percent_Identity=32.6388888888889, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 18978; Mature: 18978

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAFTEKHY
CCHHHHHHHHHHCCCCCHHEEHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
ENTILHSDQGWQYRHDSYHRFLESKGIQASMSRKGNSQDNGRMESFFGILKSEMFYGYEK
HCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFE
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCC
>Mature Secondary Structure
MEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAFTEKHY
CCHHHHHHHHHHCCCCCHHEEHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
ENTILHSDQGWQYRHDSYHRFLESKGIQASMSRKGNSQDNGRMESFFGILKSEMFYGYEK
HCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFE
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]