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The map label for this gene is manX [H]

Identifier: 225858133

GI number: 225858133

Start: 302059

End: 303048

Strand: Reverse

Name: manX [H]

Synonym: SP70585_0343

Alternate gene names: 225858133

Gene position: 303048-302059 (Counterclockwise)

Preceding gene: 225858134

Following gene: 225858132

Centisome position: 13.87

GC content: 42.63

Gene sequence:

>990_bases
ATGAGTATCGGAATCATTATTGCGAGCCACGGCGAATTTGCTGCGGGTATTCATCAGTCAGGATCTATGATCTTTGGTGA
ACAAGAAAAGGTTCAAGTTGTGACCTTTATGCCAAATGAAGGTCCTGATGATCTATACGCTAAGTTTAATAACGCTGTTG
CTGCATTTGACGCAGAAGATGAGGTTCTAGTTTTGGCTGACCTTTGGAGTGGTTCTCCATTTAACCAAGCTAGTCGCGTG
ATGGGAGAAAATCCTGAGCGTAAGTTTGCCATCATCACAGGACTTAACTTACCGATGTTGATTCAAGCCTACACAGAGCG
CCTCATGGACGCTGCTGCAGGTGTAGAAAAAGTCGCTGCTAATATCATTAAAGAAGCCAAAGATGGCATCAAAGCTCTTC
CAGAAGAGCTAAATCCAGTTGAAGAAGTTGCAAGCGCTGCAGCTGCTCCAGTTGCCCAAACTGCTATCCCAGAAGGAACT
GTTATCGGAGACGGTAAATTGAAAATCAATCTTGCCCGTCTTGACACACGTCTACTTCACGGTCAGGTTGCAACTGCTTG
GACTCCAGATTCAAAAGCAAATCGTATCATCGTTGCTTCAGATAACGTGGCTAAAGACGACCTTCGTAAAGAATTGATTA
AACAAGCAGCTCCAGGTAATGTCAAGGCTAACGTGGTTCCAATTCAAAAACTGATTGAGATTTCAAAAGACCCACGTTTT
GGAGAAACACATGCCCTTATCTTGTTTGAAACACCTCAAGATGCCCTTCGTGCCATCGAAGGCGGCGTGCCAATCAAGAC
TCTTAACGTTGGTTCTATGGCTCACTCAACAGGTAAAACATTGGTCAATACCGTTTTGTCTATGGACAAAGAAGATGTTG
CTACATTTGAAAAAATGCGTGACTTGGGTGTTGAATTTGATGTCCGTAAAGTACCAAATGATTCTAAAAAAGATTTGTTT
GACTTGATTAACAAAGCCAATGTCAAATAA

Upstream 100 bases:

>100_bases
TATGATAAATAGTTGCTTTTTTTATGAAAACGGTTTATACTTAAACAGTCTTAAAGTTTTCTTAAATGAAAACTAAAACA
AAAAAGGAGGAATGACAATA

Downstream 100 bases:

>100_bases
GCCATTATTTATGAAAGGATTTTAAACATGTCTATTATTTCTATGGTTTTAGTAGTCGTTGTAGCCTTCTTTGCAGGTCT
TGAAGGCATCCTCGACCAGT

Product: mannose-specific phosphotransferase system component iiab

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDDLYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRV
MGENPERKFAIITGLNLPMLIQAYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAPGNVKANVVPIQKLIEISKDPRF
GETHALILFETPQDALRAIEGGVPIKTLNVGSMAHSTGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLF
DLINKANVK

Sequences:

>Translated_329_residues
MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDDLYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRV
MGENPERKFAIITGLNLPMLIQAYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAPGNVKANVVPIQKLIEISKDPRF
GETHALILFETPQDALRAIEGGVPIKTLNVGSMAHSTGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLF
DLINKANVK
>Mature_328_residues
SIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDDLYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVM
GENPERKFAIITGLNLPMLIQAYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGTV
IGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAPGNVKANVVPIQKLIEISKDPRFG
ETHALILFETPQDALRAIEGGVPIKTLNVGSMAHSTGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFD
LINKANVK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=326, Percent_Identity=39.8773006134969, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI87082217, Length=155, Percent_Identity=34.1935483870968, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789527, Length=167, Percent_Identity=30.5389221556886, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR004720 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35444; Mature: 35313

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDDLYAKFNNAVAAFDAED
CCEEEEEECCCCHHHCCCCCCCEEECCCCEEEEEEECCCCCCHHHHHHHCCEEEEECCCC
EVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQAYTERLMDAAAGVEKVAA
CEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
NIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGTVIGDGKLKINLARLDTRLLH
HHHHHHHHHHHHCHHHCCHHHHHHHHHHCCHHHHCCCCCEEEECCEEEEEHHHHHHHHHC
GQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAPGNVKANVVPIQKLIEISKDPRF
CCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCC
GETHALILFETPQDALRAIEGGVPIKTLNVGSMAHSTGKTLVNTVLSMDKEDVATFEKMR
CCCEEEEEEECCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DLGVEFDVRKVPNDSKKDLFDLINKANVK
HCCCCEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDDLYAKFNNAVAAFDAED
CEEEEEECCCCHHHCCCCCCCEEECCCCEEEEEEECCCCCCHHHHHHHCCEEEEECCCC
EVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQAYTERLMDAAAGVEKVAA
CEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
NIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGTVIGDGKLKINLARLDTRLLH
HHHHHHHHHHHHCHHHCCHHHHHHHHHHCCHHHHCCCCCEEEECCEEEEEHHHHHHHHHC
GQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAPGNVKANVVPIQKLIEISKDPRF
CCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCC
GETHALILFETPQDALRAIEGGVPIKTLNVGSMAHSTGKTLVNTVLSMDKEDVATFEKMR
CCCEEEEEEECCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DLGVEFDVRKVPNDSKKDLFDLINKANVK
HCCCCEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA