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The map label for this gene is proS [H]

Identifier: 225852336

GI number: 225852336

Start: 819842

End: 821170

Strand: Direct

Name: proS [H]

Synonym: BMEA_A0862

Alternate gene names: 225852336

Gene position: 819842-821170 (Clockwise)

Preceding gene: 225852335

Following gene: 225852337

Centisome position: 38.57

GC content: 57.04

Gene sequence:

>1329_bases
ATGCGTCTGTCGCGGTATTTTTTGCCCATTCTGAAGGAAAATCCCAAGGAGGCGGAAATCGTCTCGCACAGGCTGATGCT
GCGCTCAGGCATGATCCGTCAGCAGTCTGCGGGCATCTATTCCTGGTTGCCGATTGGTTTGAAGGTGCTGAACAAGGTCT
GCACCATCATCCGTGAAGAACAGAACCGTGCGGGTGCGAACGAAATCCTGATGCCGACGATCCAGTCGGCGGACCTGTGG
CGCGAAAGCGGCCGCTATGACGCCTATGGCAAGGAAATGCTGCGCATTCAGGACCGTCAGAAGCGTGAAATGCTCTTCGG
CCCGACCAACGAGGAAATGGTTACGGATATTTTCCGTTCCTATGTGCGCTCCTACAAGGATTTGCCGCTCAATCTCTATC
ATATCCAGTGGAAGTTCCGCGATGAGGTGCGTCCGCGTTTTGGTGTCATGCGCTCACGCGAATTTCTGATGAAGGATGCC
TATTCGTTCGACCTCGATTATGAAGGCGCGAAAATGGCTTACTATCGCATGTTCGTGTCTTATCTGCGCACTTTTGCGCG
GGTTGGCTTGCAGGCCATCCCGATGCGCGCGGATACGGGGCCGATTGGCGGCGATCTTTCCCATGAATTCATCATTCTGG
CGGAAACCGGCGAAAGCCAGGTCTATTGCGACCGTGCTTATCTCGACCTTGCCGTGCCGGGTGCGGATACTGATTTCCGC
AATGACGCACAGCTGACCGATACCGTCACGCGCTGGACGACGCCTTATGCGGCAACGGACGAAATGCACGACGAAGCCGA
TTGGGCCAAGGTGAAGCCGGAAAGCCAGGTTTCCGCGCGCGGCATCGAAGTGGGCCACATTTTCCATTTCGGCACCAAAT
ATTCCGAGCCGATGGGCGCGAAAGTGCAGGGGCCGGATGGCAAGGAACATCTGGTTTCCATGGGGTCTTATGGCATCGGC
CCGTCGCGTCTGGTGGCTGCCGCCATTGAGGCTTCGCATGATGATGCGGGCATTATCTGGCCGAAGACCATTGCACCGTT
TGGTGCTGGCATCGTCAACATGAAGCCGGGCGATGAGGGATGCGATGGTGTGAGCGAAAAGCTCTACGAGGCGCTGACCA
ATGCCGGTGTCGATCCGCTGCTGGACGACAAGGATGAACGTCCGGGCGCGAAATTTGCCACGATAGATCTGATCGGCCTG
CCGACACAGGTGATCGTCGGTCCGCGCGGCGTTGCGGCTGGCGAAGTCGAGGTGAAGGACCGCAAGACGGGCGAACGCCA
GAGCCTGGGTATTGAAGCAGCAATCAACATGTTGACGGCACAAGCATGA

Upstream 100 bases:

>100_bases
TTTTCAATAGATGCATTACCCGGTAAGAAACCATGGAATATGCTTAATTTTTGCCTGATTCTTGAAGCATTCAGGCAGTT
CCTCTTGATCGGTGGTTACG

Downstream 100 bases:

>100_bases
GCAACGCGGCGGCAAAGTCCGGAGGGAAGGCCACGGCAGCCGTGAAGGCGCCGAAGTCCGGCCCTTTTTCGGCTTTTGAA
CGGATGATCGCGTGGCGATA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MRLSRYFLPILKENPKEAEIVSHRLMLRSGMIRQQSAGIYSWLPIGLKVLNKVCTIIREEQNRAGANEILMPTIQSADLW
RESGRYDAYGKEMLRIQDRQKREMLFGPTNEEMVTDIFRSYVRSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
YSFDLDYEGAKMAYYRMFVSYLRTFARVGLQAIPMRADTGPIGGDLSHEFIILAETGESQVYCDRAYLDLAVPGADTDFR
NDAQLTDTVTRWTTPYAATDEMHDEADWAKVKPESQVSARGIEVGHIFHFGTKYSEPMGAKVQGPDGKEHLVSMGSYGIG
PSRLVAAAIEASHDDAGIIWPKTIAPFGAGIVNMKPGDEGCDGVSEKLYEALTNAGVDPLLDDKDERPGAKFATIDLIGL
PTQVIVGPRGVAAGEVEVKDRKTGERQSLGIEAAINMLTAQA

Sequences:

>Translated_442_residues
MRLSRYFLPILKENPKEAEIVSHRLMLRSGMIRQQSAGIYSWLPIGLKVLNKVCTIIREEQNRAGANEILMPTIQSADLW
RESGRYDAYGKEMLRIQDRQKREMLFGPTNEEMVTDIFRSYVRSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
YSFDLDYEGAKMAYYRMFVSYLRTFARVGLQAIPMRADTGPIGGDLSHEFIILAETGESQVYCDRAYLDLAVPGADTDFR
NDAQLTDTVTRWTTPYAATDEMHDEADWAKVKPESQVSARGIEVGHIFHFGTKYSEPMGAKVQGPDGKEHLVSMGSYGIG
PSRLVAAAIEASHDDAGIIWPKTIAPFGAGIVNMKPGDEGCDGVSEKLYEALTNAGVDPLLDDKDERPGAKFATIDLIGL
PTQVIVGPRGVAAGEVEVKDRKTGERQSLGIEAAINMLTAQA
>Mature_442_residues
MRLSRYFLPILKENPKEAEIVSHRLMLRSGMIRQQSAGIYSWLPIGLKVLNKVCTIIREEQNRAGANEILMPTIQSADLW
RESGRYDAYGKEMLRIQDRQKREMLFGPTNEEMVTDIFRSYVRSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
YSFDLDYEGAKMAYYRMFVSYLRTFARVGLQAIPMRADTGPIGGDLSHEFIILAETGESQVYCDRAYLDLAVPGADTDFR
NDAQLTDTVTRWTTPYAATDEMHDEADWAKVKPESQVSARGIEVGHIFHFGTKYSEPMGAKVQGPDGKEHLVSMGSYGIG
PSRLVAAAIEASHDDAGIIWPKTIAPFGAGIVNMKPGDEGCDGVSEKLYEALTNAGVDPLLDDKDERPGAKFATIDLIGL
PTQVIVGPRGVAAGEVEVKDRKTGERQSLGIEAAINMLTAQA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=439, Percent_Identity=33.2574031890661, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI1786392, Length=262, Percent_Identity=51.5267175572519, Blast_Score=277, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI115532348, Length=348, Percent_Identity=35.3448275862069, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI193203271, Length=95, Percent_Identity=34.7368421052632, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=258, Percent_Identity=33.3333333333333, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24656200, Length=422, Percent_Identity=30.8056872037915, Blast_Score=209, Evalue=4e-54,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 49478; Mature: 49478

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFLPILKENPKEAEIVSHRLMLRSGMIRQQSAGIYSWLPIGLKVLNKVCTIIREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QNRAGANEILMPTIQSADLWRESGRYDAYGKEMLRIQDRQKREMLFGPTNEEMVTDIFRS
HHCCCCCCEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YVRSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDAYSFDLDYEGAKMAYYRMFVS
HHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YLRTFARVGLQAIPMRADTGPIGGDLSHEFIILAETGESQVYCDRAYLDLAVPGADTDFR
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCC
NDAQLTDTVTRWTTPYAATDEMHDEADWAKVKPESQVSARGIEVGHIFHFGTKYSEPMGA
CCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCHHHHCCEEEEEEEECCCCCCCCCCC
KVQGPDGKEHLVSMGSYGIGPSRLVAAAIEASHDDAGIIWPKTIAPFGAGIVNMKPGDEG
EEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCHHCCCCCCEEECCCCCCC
CDGVSEKLYEALTNAGVDPLLDDKDERPGAKFATIDLIGLPTQVIVGPRGVAAGEVEVKD
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCCCCEEEECC
RKTGERQSLGIEAAINMLTAQA
CCCCCHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MRLSRYFLPILKENPKEAEIVSHRLMLRSGMIRQQSAGIYSWLPIGLKVLNKVCTIIREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QNRAGANEILMPTIQSADLWRESGRYDAYGKEMLRIQDRQKREMLFGPTNEEMVTDIFRS
HHCCCCCCEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YVRSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDAYSFDLDYEGAKMAYYRMFVS
HHHHHCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YLRTFARVGLQAIPMRADTGPIGGDLSHEFIILAETGESQVYCDRAYLDLAVPGADTDFR
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCCCCCC
NDAQLTDTVTRWTTPYAATDEMHDEADWAKVKPESQVSARGIEVGHIFHFGTKYSEPMGA
CCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCHHHHCCEEEEEEEECCCCCCCCCCC
KVQGPDGKEHLVSMGSYGIGPSRLVAAAIEASHDDAGIIWPKTIAPFGAGIVNMKPGDEG
EEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCHHCCCCCCEEECCCCCCC
CDGVSEKLYEALTNAGVDPLLDDKDERPGAKFATIDLIGLPTQVIVGPRGVAAGEVEVKD
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCCCCEEEECC
RKTGERQSLGIEAAINMLTAQA
CCCCCHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA