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The map label for this gene is yieH [H]

Identifier: 225852024

GI number: 225852024

Start: 507908

End: 508594

Strand: Reverse

Name: yieH [H]

Synonym: BMEA_A0525

Alternate gene names: 225852024

Gene position: 508594-507908 (Counterclockwise)

Preceding gene: 225852025

Following gene: 225852020

Centisome position: 23.93

GC content: 60.12

Gene sequence:

>687_bases
ATGGCGGCACCGTCCCTCATTATTTTCGATTGCGATGGTGTTCTGGTCGATTCCGAGATCATCGCCGCAGAAGTCGAATC
CGCCCTCCTGACCGAATCCGGCTATCCGATTGCCGCCGATGAAATGGCCGAACGTTTCGCAGGCCTCACCTGGCAGGATA
TCCTGCTGACGGTGGAGCGCGCGGCGGGCATTCCGCTTTCCGCGTCGCTCCTCGACAAGTCGGAGCGCATTCTGGATGAA
AAGCTGAAGAACGAGGTCAACGCGGTGGAAGACATCGTGGAGGTGGTTTCCGCACTCAAGCTGCCGAAATGCATCTGCTC
GAACTCCACGAGCCTGCGGCTTGAAAACATGCTGAAGCGCGTCGGCCCTTACGAGCTCTTCGCACCCAATATTTTCTCCG
CCAAGGAAGTGGGCAGCGGAAAGACCAAGCCTGCCCCGGACGTGTTTCTCCACGCGGCGCGCCATTTCGGCGTCGATCCG
GCCAATGTGCTGGTGATCGAGGATTCCGTCCATGGAGTTCATGGCGCGCGCGCAGCCGGTATGCGCGTCATCGGTTTTAC
GGGCGGGGCGCACACCTATCCCGGCCATGCCGACAAGCTGACCGATGCGGGAGCCGAAACGGTTATCCACCGCCACAAGG
ATTTGCAGAGCGTGATCGACGCCCTGTCCATCTGGACAGATGCCTGA

Upstream 100 bases:

>100_bases
GTCGTTCCGGCGATTGCCGGTCGTCTTTTCCCTTTCACGCATTGCCAGACGGGAGGCCCTTCCCGCTGGATCAAAATGCT
TCAACAAGAAGGTTACGCTT

Downstream 100 bases:

>100_bases
GCACAGTCCGGAAAGAAAATTCTGACGCCTTGAATTGTCGCTCAGGCGGCAGAGCGTTTGTTGCGGCCTTTTGGCGGCGC
ATTGAGAATGGCGAGCAGTT

Product: HAD-superfamily hydrolase

Products: glycolate; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MAAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVERAAGIPLSASLLDKSERILDE
KLKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKRVGPYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDP
ANVLVIEDSVHGVHGARAAGMRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA

Sequences:

>Translated_228_residues
MAAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVERAAGIPLSASLLDKSERILDE
KLKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKRVGPYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDP
ANVLVIEDSVHGVHGARAAGMRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA
>Mature_227_residues
AAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVERAAGIPLSASLLDKSERILDEK
LKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKRVGPYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDPA
NVLVIEDSVHGVHGARAAGMRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=189, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=1e-21,
Organism=Drosophila melanogaster, GI116008157, Length=187, Percent_Identity=33.1550802139037, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17137324, Length=189, Percent_Identity=32.2751322751323, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18

Molecular weight: Translated: 24380; Mature: 24249

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVER
CCCCCEEEEECCCEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
AAGIPLSASLLDKSERILDEKLKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKR
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHH
VGPYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDPANVLVIEDSVHGVHGARAAG
CCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCC
MRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVER
CCCCEEEEECCCEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
AAGIPLSASLLDKSERILDEKLKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKR
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHH
VGPYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDPANVLVIEDSVHGVHGARAAG
CCCCEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCC
MRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-phosphoglycolate; H2O

Specific reaction: 2-phosphoglycolate + H2O = glycolate + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]