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The map label for this gene is thiE [H]

Identifier: 225851741

GI number: 225851741

Start: 224517

End: 225128

Strand: Reverse

Name: thiE [H]

Synonym: BMEA_A0221

Alternate gene names: 225851741

Gene position: 225128-224517 (Counterclockwise)

Preceding gene: 225851742

Following gene: 225851740

Centisome position: 10.59

GC content: 61.11

Gene sequence:

>612_bases
GTGACGGCACTCGACCCCTTCTACCCGATTTTCGACAGCGCGGACTGGCTGGAGCGCATGGTGCCGCTTGGCATCAGGCT
TGTGCAGTTGCGCGTCAAGGACAAGGCAGATGCGCAGCTTCGTGCCGAAATCCGTGCAGCGCGCGACATCTGCGCTGCGC
ATGACTGCCAACTCATCGTCAACGATTACTGGAAACTGGCGCTGGACGAAGGCTGCGACTTCATCCATCTCGGCCAGGAA
GACCTTGACGGTGCCGATCTCGACGCCATCCGCGCCGGTGGTTTGAAACTCGGCGTTTCAAGCCATGACGAAGCCGAACT
CGACCGCGCTTTGTCGGTCAGGCCCGACTATATCGCGCTCGGCCCCATCTATCCGACCATTCTCAAAAAGATGAAATGGC
ATGAACAGGGCTTGCCGCGCCTTGGAGAGTGGAAAGCACGGATCGGCAATATCCCGCTGGTGGGCATCGGGGGATTAAGT
GTCGAACGTGCGCCCGGCGTCTTCGCTGCTGGCGCAGATATCGTTTCCGTCGTGACCGACATCACGCTTCACGCCGACCC
GGCCGCGCGCGTTCGCGAATGGATCGCCGTCACCCGGCCTTTCGTCGCCTGA

Upstream 100 bases:

>100_bases
CGCTTGCGGTCGAGGCCGGACGGCTTGCCTATGAGGCCGACCCGATAGAAGCGCGCGACATGGCATCCCCTTCCACCCCC
CTTCTCGGAAAGGCATTCCT

Downstream 100 bases:

>100_bases
AATCACAGCTCTGGAGGAAGATCATGAAGAAAATGGCATTTGCCGCGCTGTTCGGCGCGGTGCTCAGCTTCGCCTCGTCG
GCGGAAGCCAATGACAAGCT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MTALDPFYPIFDSADWLERMVPLGIRLVQLRVKDKADAQLRAEIRAARDICAAHDCQLIVNDYWKLALDEGCDFIHLGQE
DLDGADLDAIRAGGLKLGVSSHDEAELDRALSVRPDYIALGPIYPTILKKMKWHEQGLPRLGEWKARIGNIPLVGIGGLS
VERAPGVFAAGADIVSVVTDITLHADPAARVREWIAVTRPFVA

Sequences:

>Translated_203_residues
MTALDPFYPIFDSADWLERMVPLGIRLVQLRVKDKADAQLRAEIRAARDICAAHDCQLIVNDYWKLALDEGCDFIHLGQE
DLDGADLDAIRAGGLKLGVSSHDEAELDRALSVRPDYIALGPIYPTILKKMKWHEQGLPRLGEWKARIGNIPLVGIGGLS
VERAPGVFAAGADIVSVVTDITLHADPAARVREWIAVTRPFVA
>Mature_202_residues
TALDPFYPIFDSADWLERMVPLGIRLVQLRVKDKADAQLRAEIRAARDICAAHDCQLIVNDYWKLALDEGCDFIHLGQED
LDGADLDAIRAGGLKLGVSSHDEAELDRALSVRPDYIALGPIYPTILKKMKWHEQGLPRLGEWKARIGNIPLVGIGGLSV
ERAPGVFAAGADIVSVVTDITLHADPAARVREWIAVTRPFVA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=180, Percent_Identity=46.6666666666667, Blast_Score=175, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22314; Mature: 22183

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALDPFYPIFDSADWLERMVPLGIRLVQLRVKDKADAQLRAEIRAARDICAAHDCQLIV
CCCCCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHH
NDYWKLALDEGCDFIHLGQEDLDGADLDAIRAGGLKLGVSSHDEAELDRALSVRPDYIAL
HHHHHHHHHCCCCEEECCCCCCCCCCHHHHHCCCEEECCCCCCHHHHHHHHCCCCCEEEE
GPIYPTILKKMKWHEQGLPRLGEWKARIGNIPLVGIGGLSVERAPGVFAAGADIVSVVTD
CCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCEEECCHHHHHHHHH
ITLHADPAARVREWIAVTRPFVA
HHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TALDPFYPIFDSADWLERMVPLGIRLVQLRVKDKADAQLRAEIRAARDICAAHDCQLIV
CCCCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHH
NDYWKLALDEGCDFIHLGQEDLDGADLDAIRAGGLKLGVSSHDEAELDRALSVRPDYIAL
HHHHHHHHHCCCCEEECCCCCCCCCCHHHHHCCCEEECCCCCCHHHHHHHHCCCCCEEEE
GPIYPTILKKMKWHEQGLPRLGEWKARIGNIPLVGIGGLSVERAPGVFAAGADIVSVVTD
CCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCEEECCHHHHHHHHH
ITLHADPAARVREWIAVTRPFVA
HHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]