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The map label for this gene is bioB [H]

Identifier: 225686291

GI number: 225686291

Start: 455146

End: 456186

Strand: Reverse

Name: bioB [H]

Synonym: BMEA_B0469

Alternate gene names: 225686291

Gene position: 456186-455146 (Counterclockwise)

Preceding gene: 225686293

Following gene: 225686290

Centisome position: 38.48

GC content: 59.65

Gene sequence:

>1041_bases
ATGCCCTGCACCGTGGAAAAGCACGAGAAACCGGCCGCCAAGGCCGCTCAGGCTATGCCAGATCGTGGAGGCGAGAACGG
GGCATCCTGTTCGGTGGGCCGTTGGAGCGCAGAAGAAGCGCGGGCAATCTATAATCTGCCGTTCAATGATCTGCTCTTTC
GCGCCCACGGCCTGCACCGCGAGAATTTTGATCCGAACCGCATCCAGCTCAGCAAGCTTCTCAACATCAAGACGGGTGGA
TGCCCGGAAGATTGCGGTTATTGCAGCCAGTCTGCTTCTGCGGAAAATGGCCTGAAAGCCTCCAAACTCATGGAAATCGA
AACCGTTCTGGAGGAGGCGCGCAAGGCCAAGGCTGCGGGTGCCACGCGCTATTGCATGGGGGCAGCTTGGCGCAGCCCCA
AGGATCGCGACATGCCCGCCCTCACCCATATGATCGAAAGCGTGAAGGCAATGGGCCTGGAAACCTGCATGACGCTGGGG
ATGCTGGATAGCGACAAGGCGGAAAAACTCGCCGATGCAGGCCTCGATTATTACAATCACAATATCGATACGTCCGAGCG
TTTTTATCCCGCAGTCATCACCACGCGCAGTTTTGAAGATCGGCTCGATACGCTTGCAAATGTGCGCAATGCGGGCATCA
AGGTTTGCAGCGGCGGTATTCTGGGGCTTGGCGAAGAGGCCGAAGATCGCATCGACATGCTGGTAACACTGGCCAACCTG
CCGGAACCGCCGGAGAGCGTGCCGATCAATATGCTGATCCCCATGCCGGGCACACGGCTGGCAAAGGCCGCGCCAGTCGA
TCCGCTTGAATTTGTCCGTGTGGTCGCGCTGGCGCGCATTCTCATGCCGAAATCCCATGTGCGCCTGACGGCTGGCCGTA
CCGCCATGAGCGACGAGATGCAGGCGCTCTGCTTCTTTGCCGGAGCCAATTCGCTATTTATGGGCGACACCTTGCTCACA
GCCGCCAACCCCGGCGACGACCGCGATTCCAGCCTGCTGCGCCGCCTCGGCATACAGGCGGAAACGGAGCAGCCTGCGTG
A

Upstream 100 bases:

>100_bases
ATCAAAATTATCTATTATATTATCATTTGTGGATTCACATAAAACTGATTTATCTATTTTTGCAGATTGATTCTGTTTTT
ATCTACAATTTGGAGGAAGA

Downstream 100 bases:

>100_bases
AACTCGACACCTACCTGACAAGGCTGGAAAATCTGGAGCGCAAGGGGCGGCGGCGCAATCTTGCGCTGGCCGATGGGCTT
GATTTCAGTTCCAATGATTA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MPCTVEKHEKPAAKAAQAMPDRGGENGASCSVGRWSAEEARAIYNLPFNDLLFRAHGLHRENFDPNRIQLSKLLNIKTGG
CPEDCGYCSQSASAENGLKASKLMEIETVLEEARKAKAAGATRYCMGAAWRSPKDRDMPALTHMIESVKAMGLETCMTLG
MLDSDKAEKLADAGLDYYNHNIDTSERFYPAVITTRSFEDRLDTLANVRNAGIKVCSGGILGLGEEAEDRIDMLVTLANL
PEPPESVPINMLIPMPGTRLAKAAPVDPLEFVRVVALARILMPKSHVRLTAGRTAMSDEMQALCFFAGANSLFMGDTLLT
AANPGDDRDSSLLRRLGIQAETEQPA

Sequences:

>Translated_346_residues
MPCTVEKHEKPAAKAAQAMPDRGGENGASCSVGRWSAEEARAIYNLPFNDLLFRAHGLHRENFDPNRIQLSKLLNIKTGG
CPEDCGYCSQSASAENGLKASKLMEIETVLEEARKAKAAGATRYCMGAAWRSPKDRDMPALTHMIESVKAMGLETCMTLG
MLDSDKAEKLADAGLDYYNHNIDTSERFYPAVITTRSFEDRLDTLANVRNAGIKVCSGGILGLGEEAEDRIDMLVTLANL
PEPPESVPINMLIPMPGTRLAKAAPVDPLEFVRVVALARILMPKSHVRLTAGRTAMSDEMQALCFFAGANSLFMGDTLLT
AANPGDDRDSSLLRRLGIQAETEQPA
>Mature_345_residues
PCTVEKHEKPAAKAAQAMPDRGGENGASCSVGRWSAEEARAIYNLPFNDLLFRAHGLHRENFDPNRIQLSKLLNIKTGGC
PEDCGYCSQSASAENGLKASKLMEIETVLEEARKAKAAGATRYCMGAAWRSPKDRDMPALTHMIESVKAMGLETCMTLGM
LDSDKAEKLADAGLDYYNHNIDTSERFYPAVITTRSFEDRLDTLANVRNAGIKVCSGGILGLGEEAEDRIDMLVTLANLP
EPPESVPINMLIPMPGTRLAKAAPVDPLEFVRVVALARILMPKSHVRLTAGRTAMSDEMQALCFFAGANSLFMGDTLLTA
ANPGDDRDSSLLRRLGIQAETEQPA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=59.0163934426229, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321725, Length=313, Percent_Identity=47.2843450479233, Blast_Score=315, Evalue=7e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37545; Mature: 37414

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCTVEKHEKPAAKAAQAMPDRGGENGASCSVGRWSAEEARAIYNLPFNDLLFRAHGLHR
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHEEECCCHHHHHHHHHCCCC
ENFDPNRIQLSKLLNIKTGGCPEDCGYCSQSASAENGLKASKLMEIETVLEEARKAKAAG
CCCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ATRYCMGAAWRSPKDRDMPALTHMIESVKAMGLETCMTLGMLDSDKAEKLADAGLDYYNH
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCC
NIDTSERFYPAVITTRSFEDRLDTLANVRNAGIKVCSGGILGLGEEAEDRIDMLVTLANL
CCCCCCCEECCEEECCCHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCC
PEPPESVPINMLIPMPGTRLAKAAPVDPLEFVRVVALARILMPKSHVRLTAGRTAMSDEM
CCCCCCCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
QALCFFAGANSLFMGDTLLTAANPGDDRDSSLLRRLGIQAETEQPA
HHHHHHHCCCCEEECCEEEECCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PCTVEKHEKPAAKAAQAMPDRGGENGASCSVGRWSAEEARAIYNLPFNDLLFRAHGLHR
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHEEECCCHHHHHHHHHCCCC
ENFDPNRIQLSKLLNIKTGGCPEDCGYCSQSASAENGLKASKLMEIETVLEEARKAKAAG
CCCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ATRYCMGAAWRSPKDRDMPALTHMIESVKAMGLETCMTLGMLDSDKAEKLADAGLDYYNH
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCC
NIDTSERFYPAVITTRSFEDRLDTLANVRNAGIKVCSGGILGLGEEAEDRIDMLVTLANL
CCCCCCCEECCEEECCCHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCC
PEPPESVPINMLIPMPGTRLAKAAPVDPLEFVRVVALARILMPKSHVRLTAGRTAMSDEM
CCCCCCCCEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
QALCFFAGANSLFMGDTLLTAANPGDDRDSSLLRRLGIQAETEQPA
HHHHHHHCCCCEEECCEEEECCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA