Click here to switch to the map view.

The map label for this gene is def [C]

Identifier: 225686258

GI number: 225686258

Start: 423405

End: 423899

Strand: Reverse

Name: def [C]

Synonym: BMEA_B0433

Alternate gene names: 225686258

Gene position: 423899-423405 (Counterclockwise)

Preceding gene: 225686259

Following gene: 225686255

Centisome position: 35.76

GC content: 57.58

Gene sequence:

>495_bases
ATGACCGTTCGCCTGATCGTGAAATATCCTGACCCGCGCCTGCGGGCCGCAGCCGAGCCAGTGACCACCTTTGATGAGGG
TCTTCGCAAATTGGCGGACGATCTTCTGGATACGATGCGCGCCGCGCCCGGCATTGGCATCACCGCCCCGCATATCGGCA
TCTCAAAGAGAGTGGTGGTTCTGGAACTCGACAGGGCAGCCGGGCCTAAGATCTATATAAACCCTGAAATCGTCTGGGCT
TGCGAGGAGAAAATCCGCCATCAGGAAGGAAGCGTATCGATGCCGGGCGTGGTTGACGAGGTTGAGCGCCATGCGCGAAT
TCGGCTTCGCTATCAAGATCTGGACGGGAATGAGCAGACTGAGGAATCCGACGGACTGCTTGCCGTCTGCCATCAGCACG
AAATCGACCAGCTTGACGGCATATTCTGGGTGCAGCGGCTTTCCCGTCTCAGGAGGGAGCGCCTCATAAAGCGCTATGAG
AAGCTACAGCGATAA

Upstream 100 bases:

>100_bases
AATCCTCGTTTCCTACCAGTCGGCTTATGCTCCAGGCGACATGGATGGCTCCATTTGCTATCGTCGGAATGAAAATTATG
ACGCCTGCCAGAGAGACAAG

Downstream 100 bases:

>100_bases
GATGCTGAAGTGAAAGGAGCAGCCCGTTGAACCGGGCCGCTCCGGTCTTTCATCAGTATAGCAGGCGAGCGCGAATCGTG
CCGGGAATTTCGCGGATTTC

Product: peptide deformylase

Products: Formate; L-Methionine. [C]

Alternate protein names: Polypeptide deformylase-like

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTVRLIVKYPDPRLRAAAEPVTTFDEGLRKLADDLLDTMRAAPGIGITAPHIGISKRVVVLELDRAAGPKIYINPEIVWA
CEEKIRHQEGSVSMPGVVDEVERHARIRLRYQDLDGNEQTEESDGLLAVCHQHEIDQLDGIFWVQRLSRLRRERLIKRYE
KLQR

Sequences:

>Translated_164_residues
MTVRLIVKYPDPRLRAAAEPVTTFDEGLRKLADDLLDTMRAAPGIGITAPHIGISKRVVVLELDRAAGPKIYINPEIVWA
CEEKIRHQEGSVSMPGVVDEVERHARIRLRYQDLDGNEQTEESDGLLAVCHQHEIDQLDGIFWVQRLSRLRRERLIKRYE
KLQR
>Mature_163_residues
TVRLIVKYPDPRLRAAAEPVTTFDEGLRKLADDLLDTMRAAPGIGITAPHIGISKRVVVLELDRAAGPKIYINPEIVWAC
EEKIRHQEGSVSMPGVVDEVERHARIRLRYQDLDGNEQTEESDGLLAVCHQHEIDQLDGIFWVQRLSRLRRERLIKRYEK
LQR

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=35.1515151515151, Blast_Score=103, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24645728, Length=164, Percent_Identity=29.2682926829268, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEFL_BRUME (P63919)

Other databases:

- EMBL:   AE008918
- PIR:   AC3611
- RefSeq:   NP_541790.2
- ProteinModelPortal:   P63919
- SMR:   P63919
- GeneID:   1198584
- GenomeReviews:   AE008918_GR
- KEGG:   bme:BMEII0812
- HOGENOM:   HBG665227
- OMA:   DFLAICI
- ProtClustDB:   PRK12846
- BioCyc:   BMEL224914:BMEII0812-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: 3.5.1.88 [C]

Molecular weight: Translated: 18857; Mature: 18726

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRLIVKYPDPRLRAAAEPVTTFDEGLRKLADDLLDTMRAAPGIGITAPHIGISKRVVV
CEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LELDRAAGPKIYINPEIVWACEEKIRHQEGSVSMPGVVDEVERHARIRLRYQDLDGNEQT
EEECCCCCCEEEECCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCC
EESDGLLAVCHQHEIDQLDGIFWVQRLSRLRRERLIKRYEKLQR
CCCCCEEEEECHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVRLIVKYPDPRLRAAAEPVTTFDEGLRKLADDLLDTMRAAPGIGITAPHIGISKRVVV
EEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
LELDRAAGPKIYINPEIVWACEEKIRHQEGSVSMPGVVDEVERHARIRLRYQDLDGNEQT
EEECCCCCCEEEECCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCC
EESDGLLAVCHQHEIDQLDGIFWVQRLSRLRRERLIKRYEKLQR
CCCCCEEEEECHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Formyl-L-Methionine; H2O [C]

Specific reaction: N-Formyl-L-Methionine + H2O = Formate + L-Methionine. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; EDTA; o-Phenantroline [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688