BacMap

Definition	Brucella melitensis ATCC 23457 chromosome chromosome II, complete sequence.
Accession	NC_012442
Length	1,185,518

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The map label for this gene is fabG2 [H]

Identifier: 225686255

GI number: 225686255

Start: 418325

End: 419092

Strand: Reverse

Name: fabG2 [H]

Synonym: BMEA_B0428

Alternate gene names: 225686255

Gene position: 419092-418325 (Counterclockwise)

Preceding gene: 225686258

Following gene: 225686254

Centisome position: 35.35

GC content: 61.33

Gene sequence:

>768_bases
ATGCAGATAGAAAATCGCGTATTTCTGATTACCGGCGCAGGCTCCGGCCTTGGCGCTGCCGTTTCCAAAATGGCTGTTGA
GGCGGGCGCAAAGGTGGTCCTTCTCGATGTGAATGCGGAAGCAGGCGAAGCGGGCGCGAAGGCGCTTGGCGCTTCGGCCA
GATTCCAGCGCACAGACGTCGCAAGCGATACCGACGGCAAGGCTGCAATTGCAGCAGCGATTGAAGCTTTTAGCCGCATC
GACGTACTGGTCAATTGCGCCGGCGTCGCGCCGGGCGAAAAGGTGCTTGGCCGCGAAGGCGCACACAAGCTTGAAACCTT
CACCCGCACCATTTCGATCAACCTGATCGGCACCTTCAACATGCTGCGCCTTGCAGCCGAAGCCATGGCGAAGAACGAAC
CCGGCCAGGGCGGCGAGCGCGGTGTGATCATCAACACGGCTTCCGTTGCCGCTTTTGACGGCCAGATCGGCCAGGCTGCC
TATTCGGCCTCCAAGGGTGGCGTTGCGGCCATGACGCTACCCGTCGCGCGCGAACTTGCCCGCCACGGCATTCGTGTGAT
GACCATTGCGCCCGGCATTTTCAAGACCCCGATGATGGCAGGAATGCCGCAGGAAGTGCAGGACGCGCTGGGTGCTTCCG
TGCCTTTCCCACCGCGTCTTGGCGAACCGGCGGAATATGCCGCCCTCGTCCGCCATATCGTGGAAAACCAGATGTTGAAC
GGCGAAGTGATCCGTCTCGACGGCGCACTGCGTATGGCCGCCAAGTAA

Upstream 100 bases:

>100_bases
GGCGGAGCTTCCCAAGACGCCGAGCGGAAAAATACAGCGCTTCCTCCTGCGCAAGGCGGAAGTCGAAAAGCAACAGCAAC
AGAATTAGGGCCTGAAGCAA

Downstream 100 bases:

>100_bases
AGGCCGCGAAGTAAAGCCAAGGCAATTTCTGACGAAATTGCGCACAGACAAAGCAGCGAGGAACAGTCATGTCCAAGAGC
GATCCTATTGTCATCGTCGG

Product: 3-hydroxyacyl-CoA dehydrogenase type-2

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MQIENRVFLITGAGSGLGAAVSKMAVEAGAKVVLLDVNAEAGEAGAKALGASARFQRTDVASDTDGKAAIAAAIEAFSRI
DVLVNCAGVAPGEKVLGREGAHKLETFTRTISINLIGTFNMLRLAAEAMAKNEPGQGGERGVIINTASVAAFDGQIGQAA
YSASKGGVAAMTLPVARELARHGIRVMTIAPGIFKTPMMAGMPQEVQDALGASVPFPPRLGEPAEYAALVRHIVENQMLN
GEVIRLDGALRMAAK

Sequences:

>Translated_255_residues
MQIENRVFLITGAGSGLGAAVSKMAVEAGAKVVLLDVNAEAGEAGAKALGASARFQRTDVASDTDGKAAIAAAIEAFSRI
DVLVNCAGVAPGEKVLGREGAHKLETFTRTISINLIGTFNMLRLAAEAMAKNEPGQGGERGVIINTASVAAFDGQIGQAA
YSASKGGVAAMTLPVARELARHGIRVMTIAPGIFKTPMMAGMPQEVQDALGASVPFPPRLGEPAEYAALVRHIVENQMLN
GEVIRLDGALRMAAK
>Mature_255_residues
MQIENRVFLITGAGSGLGAAVSKMAVEAGAKVVLLDVNAEAGEAGAKALGASARFQRTDVASDTDGKAAIAAAIEAFSRI
DVLVNCAGVAPGEKVLGREGAHKLETFTRTISINLIGTFNMLRLAAEAMAKNEPGQGGERGVIINTASVAAFDGQIGQAA
YSASKGGVAAMTLPVARELARHGIRVMTIAPGIFKTPMMAGMPQEVQDALGASVPFPPRLGEPAEYAALVRHIVENQMLN
GEVIRLDGALRMAAK

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=252, Percent_Identity=57.1428571428571, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI83715985, Length=252, Percent_Identity=53.5714285714286, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI15277342, Length=270, Percent_Identity=34.4444444444444, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI40254992, Length=248, Percent_Identity=29.4354838709677, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI31542939, Length=262, Percent_Identity=27.8625954198473, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI59889578, Length=216, Percent_Identity=31.0185185185185, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4504505, Length=219, Percent_Identity=31.0502283105023, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI224922801, Length=177, Percent_Identity=32.7683615819209, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI32483357, Length=253, Percent_Identity=28.0632411067194, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI10190704, Length=194, Percent_Identity=30.4123711340206, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI5031737, Length=197, Percent_Identity=28.4263959390863, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=28.4263959390863, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=29.1338582677165, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=27.9693486590038, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788459, Length=257, Percent_Identity=28.4046692607004, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082160, Length=257, Percent_Identity=27.6264591439689, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790717, Length=256, Percent_Identity=28.515625, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787905, Length=231, Percent_Identity=30.7359307359307, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1789208, Length=253, Percent_Identity=30.0395256916996, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789057, Length=220, Percent_Identity=28.6363636363636, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786812, Length=198, Percent_Identity=31.3131313131313, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367175, Length=254, Percent_Identity=29.9212598425197, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787820, Length=189, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=250, Percent_Identity=48, Blast_Score=228, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI25147288, Length=252, Percent_Identity=29.7619047619048, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=31.1023622047244, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17561402, Length=264, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=221, Percent_Identity=28.9592760180996, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560676, Length=259, Percent_Identity=25.8687258687259, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=269, Percent_Identity=26.7657992565056, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=209, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=263, Percent_Identity=25.0950570342205, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17563726, Length=211, Percent_Identity=27.4881516587678, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=265, Percent_Identity=25.6603773584906, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=206, Percent_Identity=27.1844660194175, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=207, Percent_Identity=28.0193236714976, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=195, Percent_Identity=28.2051282051282, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17564282, Length=208, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=214, Percent_Identity=26.1682242990654, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=207, Percent_Identity=26.0869565217391, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=221, Percent_Identity=28.5067873303167, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17551412, Length=233, Percent_Identity=27.4678111587983, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=268, Percent_Identity=25.7462686567164, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193203115, Length=206, Percent_Identity=26.2135922330097, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=204, Percent_Identity=32.3529411764706, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17737361, Length=252, Percent_Identity=55.5555555555556, Blast_Score=279, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24639444, Length=255, Percent_Identity=33.7254901960784, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21357041, Length=229, Percent_Identity=30.5676855895196, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI23397609, Length=258, Percent_Identity=29.4573643410853, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24644339, Length=231, Percent_Identity=29.8701298701299, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28571526, Length=244, Percent_Identity=27.8688524590164, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI18859837, Length=200, Percent_Identity=30, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221331218, Length=203, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665243, Length=203, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI221331216, Length=204, Percent_Identity=28.921568627451, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665247, Length=204, Percent_Identity=28.921568627451, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356301, Length=204, Percent_Identity=29.9019607843137, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571387, Length=219, Percent_Identity=28.310502283105, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665264, Length=202, Percent_Identity=28.7128712871287, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 26264; Mature: 26264

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIENRVFLITGAGSGLGAAVSKMAVEAGAKVVLLDVNAEAGEAGAKALGASARFQRTDV
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCEECCCC
ASDTDGKAAIAAAIEAFSRIDVLVNCAGVAPGEKVLGREGAHKLETFTRTISINLIGTFN
CCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHCCCCHHHHHHHEEEEEEEEEEHHH
MLRLAAEAMAKNEPGQGGERGVIINTASVAAFDGQIGQAAYSASKGGVAAMTLPVARELA
HHHHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCHHHHCCCCCCEEEEHHHHHHHHH
RHGIRVMTIAPGIFKTPMMAGMPQEVQDALGASVPFPPRLGEPAEYAALVRHIVENQMLN
HCCEEEEEECCCHHHCCHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
GEVIRLDGALRMAAK
CEEEEECCCEEECCC
>Mature Secondary Structure
MQIENRVFLITGAGSGLGAAVSKMAVEAGAKVVLLDVNAEAGEAGAKALGASARFQRTDV
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCEECCCC
ASDTDGKAAIAAAIEAFSRIDVLVNCAGVAPGEKVLGREGAHKLETFTRTISINLIGTFN
CCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHHCCCCHHHHHHHEEEEEEEEEEHHH
MLRLAAEAMAKNEPGQGGERGVIINTASVAAFDGQIGQAAYSASKGGVAAMTLPVARELA
HHHHHHHHHHCCCCCCCCCCEEEEEECEEEEECCCCCHHHHCCCCCCEEEEHHHHHHHHH
RHGIRVMTIAPGIFKTPMMAGMPQEVQDALGASVPFPPRLGEPAEYAALVRHIVENQMLN
HCCEEEEEECCCHHHCCHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
GEVIRLDGALRMAAK
CEEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]