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The map label for this gene is groEL

Identifier: 225686050

GI number: 225686050

Start: 178940

End: 180580

Strand: Reverse

Name: groEL

Synonym: BMEA_B0195

Alternate gene names: 225686050

Gene position: 180580-178940 (Counterclockwise)

Preceding gene: 225686051

Following gene: 225686049

Centisome position: 15.23

GC content: 58.99

Gene sequence:

>1641_bases
ATGGCTGCAAAAGACGTAAAATTCGGCCGCACTGCGCGCGAAAAGATGCTGCGCGGCGTCGATATCCTCGCTGACGCTGT
TAAGGTCACGCTCGGCCCGAAGGGCCGCAATGTCGTTATCGAGAAGTCCTTCGGCGCTCCGCGCATCACCAAGGACGGCG
TTTCGGTCGCCAAGGAAGTCGAACTGGAAGACAAGTTTGAAAACATGGGCGCACAGATGCTGCGCGAAGTGGCTTCCAAG
ACCAACGATACTGCCGGTGACGGCACCACGACCGCGACCGTTCTCGGTCAGGCCATCGTTCAGGAAGGCGCCAAGGCCGT
TGCCGCTGGCATGAACCCGATGGACCTGAAGCGCGGCATCGACCTCGCTGTCAACGAAGTTGTGGCTGAGCTGCTGAAGA
AGGCCAAAAAGATCAACACTTCGGAAGAAGTTGCCCAGGTTGGCACCATCTCTGCCAACGGCGAAGCCGAAATCGGCAAG
ATGATCGCCGAAGCGATGCAGAAGGTCGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAGACCGCCGAAACCGAACT
CGAAGTCGTCGAAGGCATGCAGTTCGACCGCGGCTACCTGTCGCCTTACTTCGTCACCAACCCTGAAAAGATGGTTGCTG
ACCTCGAAGACGCCTACATCCTTCTGCACGAAAAGAAGCTCTCGAACCTTCAGGCTCTCCTGCCGGTTCTCGAAGCTGTC
GTCCAGACCTCCAAGCCGCTTCTCATCATTGCTGAAGACGTGGAAGGCGAAGCTCTTGCAACGCTCGTCGTCAACAAGCT
GCGCGGCGGCCTGAAGATTGCTGCCGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTCGAAGACATCGCGA
TCCTCACTGGCGGTCAGGTCATCTCCGAAGACCTCGGCATCAAGCTTGAAAGCGTTACGCTCGACATGCTGGGCCGCGCC
AAGAAGGTTTCGATCTCCAAGGAAAACACGACGATCGTTGACGGTGCAGGCCAGAAGGCCGAAATCGACGCTCGCGTTGG
CCAGATCAAGCAGCAGATCGAAGAAACCACTTCGGACTACGACCGTGAAAAGCTTCAGGAACGTCTTGCCAAGCTCGCTG
GCGGCGTTGCCGTGATCCGCGTCGGCGGTGCAACGGAAGTTGAAGTGAAGGAAAAGAAGGACCGCGTTGACGACGCCCTG
AACGCAACCCGCGCTGCGGTTGAAGAAGGTATCGTTGCCGGCGGCGGCACCGCCCTGCTCCGCGCTTCGACCAAGATCAC
CGCAAAGGGTGTGAATGCCGACCAGGAAGCTGGCATCAACATCGTTCGTCGCGCGATCCAGGCTCCGGCCCGCCAGATCA
CGACCAATGCCGGTGAAGAAGCTTCGGTCATCGTTGGCAAGATCCTCGAAAACACGTCCGAAACCTTCGGCTACAACACG
GCCAATGGCGAATATGGCGACCTGATCTCGCTCGGCATTGTTGACCCGGTCAAGGTTGTCCGCACGGCTCTGCAGAACGC
AGCTTCGGTTGCCGGCCTGCTGATCACGACGGAAGCAATGATCGCCGAACTGCCGAAGAAGGACGCAGCTCCGGCTGGCA
TGCCTGGCGGTATGGGTGGCATGGGCGGCATGGACTTCTAA

Upstream 100 bases:

>100_bases
TGTCGGCTAAAAAATTCCTTTTGCGTTGCTTCACGCAACCAGAATTCCATTCACAGTACAACATTTACAAATCTGACCGG
GATATTCCCAGGAGAGTAAA

Downstream 100 bases:

>100_bases
GAAGTCCATGCCACCGCAGGACAGGCGAGAAAGCCTGCCCCACTGCGACCAGAAATTTAAAAACCTCCGGTTCCCGCCGG
AGGTTTTTTATTCGCTAAAA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MAAKDVKFGRTAREKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVSVAKEVELEDKFENMGAQMLREVASK
TNDTAGDGTTTATVLGQAIVQEGAKAVAAGMNPMDLKRGIDLAVNEVVAELLKKAKKINTSEEVAQVGTISANGEAEIGK
MIAEAMQKVGNEGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNPEKMVADLEDAYILLHEKKLSNLQALLPVLEAV
VQTSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGIKLESVTLDMLGRA
KKVSISKENTTIVDGAGQKAEIDARVGQIKQQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDAL
NATRAAVEEGIVAGGGTALLRASTKITAKGVNADQEAGINIVRRAIQAPARQITTNAGEEASVIVGKILENTSETFGYNT
ANGEYGDLISLGIVDPVKVVRTALQNAASVAGLLITTEAMIAELPKKDAAPAGMPGGMGGMGGMDF

Sequences:

>Translated_546_residues
MAAKDVKFGRTAREKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVSVAKEVELEDKFENMGAQMLREVASK
TNDTAGDGTTTATVLGQAIVQEGAKAVAAGMNPMDLKRGIDLAVNEVVAELLKKAKKINTSEEVAQVGTISANGEAEIGK
MIAEAMQKVGNEGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNPEKMVADLEDAYILLHEKKLSNLQALLPVLEAV
VQTSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGIKLESVTLDMLGRA
KKVSISKENTTIVDGAGQKAEIDARVGQIKQQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDAL
NATRAAVEEGIVAGGGTALLRASTKITAKGVNADQEAGINIVRRAIQAPARQITTNAGEEASVIVGKILENTSETFGYNT
ANGEYGDLISLGIVDPVKVVRTALQNAASVAGLLITTEAMIAELPKKDAAPAGMPGGMGGMGGMDF
>Mature_545_residues
AAKDVKFGRTAREKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVSVAKEVELEDKFENMGAQMLREVASKT
NDTAGDGTTTATVLGQAIVQEGAKAVAAGMNPMDLKRGIDLAVNEVVAELLKKAKKINTSEEVAQVGTISANGEAEIGKM
IAEAMQKVGNEGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNPEKMVADLEDAYILLHEKKLSNLQALLPVLEAVV
QTSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGIKLESVTLDMLGRAK
KVSISKENTTIVDGAGQKAEIDARVGQIKQQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALN
ATRAAVEEGIVAGGGTALLRASTKITAKGVNADQEAGINIVRRAIQAPARQITTNAGEEASVIVGKILENTSETFGYNTA
NGEYGDLISLGIVDPVKVVRTALQNAASVAGLLITTEAMIAELPKKDAAPAGMPGGMGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=54.2372881355932, Blast_Score=576, Evalue=1e-164,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=54.2372881355932, Blast_Score=576, Evalue=1e-164,
Organism=Homo sapiens, GI5453607, Length=535, Percent_Identity=23.1775700934579, Blast_Score=83, Evalue=8e-16,
Organism=Escherichia coli, GI1790586, Length=531, Percent_Identity=68.361581920904, Blast_Score=710, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=53.4971644612476, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI193210679, Length=240, Percent_Identity=49.1666666666667, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17564182, Length=553, Percent_Identity=22.7848101265823, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=57.9847908745247, Blast_Score=603, Evalue=1e-173,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=56.0606060606061, Blast_Score=587, Evalue=1e-168,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=56.0606060606061, Blast_Score=587, Evalue=1e-168,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=53.3333333333333, Blast_Score=563, Evalue=1e-160,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=53.3333333333333, Blast_Score=563, Evalue=1e-160,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=53.3333333333333, Blast_Score=563, Evalue=1e-160,
Organism=Drosophila melanogaster, GI17864606, Length=555, Percent_Identity=44.8648648648649, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24584129, Length=533, Percent_Identity=36.0225140712946, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI19921262, Length=533, Percent_Identity=36.0225140712946, Blast_Score=333, Evalue=2e-91,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_BRUA1 (B2SCZ4)

Other databases:

- EMBL:   M82975
- EMBL:   M83930
- EMBL:   CP000888
- PIR:   B43827
- PIR:   S22347
- RefSeq:   YP_001932144.1
- ProteinModelPortal:   B2SCZ4
- SMR:   B2SCZ4
- GeneID:   6326458
- GenomeReviews:   CP000888_GR
- KEGG:   bmc:BAbS19_II01790
- HOGENOM:   HBG625289
- OMA:   NSDTSIG
- ProtClustDB:   PRK00013
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57516; Mature: 57385

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVKFGRTAREKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVSVAKEV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCHHHHHHHHHH
ELEDKFENMGAQMLREVASKTNDTAGDGTTTATVLGQAIVQEGAKAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
DLAVNEVVAELLKKAKKINTSEEVAQVGTISANGEAEIGKMIAEAMQKVGNEGVITVEEA
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELEVVEGMQFDRGYLSPYFVTNPEKMVADLEDAYILLHEKKLSNLQALLPVLEAV
CCHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHH
VQTSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGQV
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKLESVTLDMLGRAKKVSISKENTTIVDGAGQKAEIDARVGQIKQQIEETTSDY
HHHHHCCEEEEEHHHHHCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNATRAAVEEGIVAGGGTALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
RASTKITAKGVNADQEAGINIVRRAIQAPARQITTNAGEEASVIVGKILENTSETFGYNT
EECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHCCCCC
ANGEYGDLISLGIVDPVKVVRTALQNAASVAGLLITTEAMIAELPKKDAAPAGMPGGMGG
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MGGMDF
CCCCCC
>Mature Secondary Structure 
AAKDVKFGRTAREKMLRGVDILADAVKVTLGPKGRNVVIEKSFGAPRITKDGVSVAKEV
CCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCHHHHHHHHHH
ELEDKFENMGAQMLREVASKTNDTAGDGTTTATVLGQAIVQEGAKAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
DLAVNEVVAELLKKAKKINTSEEVAQVGTISANGEAEIGKMIAEAMQKVGNEGVITVEEA
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELEVVEGMQFDRGYLSPYFVTNPEKMVADLEDAYILLHEKKLSNLQALLPVLEAV
CCHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHH
VQTSKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTGGQV
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKLESVTLDMLGRAKKVSISKENTTIVDGAGQKAEIDARVGQIKQQIEETTSDY
HHHHHCCEEEEEHHHHHCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNATRAAVEEGIVAGGGTALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
RASTKITAKGVNADQEAGINIVRRAIQAPARQITTNAGEEASVIVGKILENTSETFGYNT
EECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHCCCCC
ANGEYGDLISLGIVDPVKVVRTALQNAASVAGLLITTEAMIAELPKKDAAPAGMPGGMGG
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MGGMDF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1347461; 1350274