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The map label for this gene is hisZ [H]

Identifier: 225686043

GI number: 225686043

Start: 172541

End: 173671

Strand: Direct

Name: hisZ [H]

Synonym: BMEA_B0188

Alternate gene names: 225686043

Gene position: 172541-173671 (Clockwise)

Preceding gene: 225686042

Following gene: 225686044

Centisome position: 14.55

GC content: 60.39

Gene sequence:

>1131_bases
ATGGTTGGATCGCGCACATCGCCGATATTTAATGCGCTCCGCGTAGAGCTTAATGCGCGCGAAGCCGAACTGGTGGAAAT
TCCGCTGATCCAGCCTGCCGATCCCTTTCTGGATATGGCGGGTGAAGATCTGCGCCGGCGTATCTTCCTGACCGAGAATG
AAAACGGTGACAGCCTTTGCCTGCGGCCGGAATTCACCATTCCGGTCTGCCGCAACCATATCGCGCTCAATGCCGCGACG
CCCAAACGCTATGCCTATCTGGGCGAAGTGTTTCGCCAGCGCCGCGATGGTGCTGCCGAATTTTTGCAGGCGGGCATAGA
AGATCTCGGTGCTGCCGACGAGGCCGCTTCCGATGCGCGCTCGCTTGCCGATGCGCTCTCCTGCGTGAAGGCAATCGCAC
CTGACGCACCGCTTGAAATCGTGCTGGGCGACCAGTCGGTTTTCGCAGGCATGTTGAAGGCGCTCGGCTTGCCGCAAGGC
TGGCGGAAGAAATTGCTGCGCTCCTTCGGGGATGCGCACTCCATGGATCTGGCGCTTGCCGAACTGACGGGCACGCAGCG
GCGCGATCCGCTGCCTGAAAGCCTTGCCGTTCTGGTTGCCGAAGGCGATGAGATCGGCCTTGCCCGTATGCTGGAGGCTG
AAATGCTGGAAGCGGGCATTTCGCCCGGCGCGGGCCGCACACCTGTGGAAATTGCCCGCCGCCTCATCGAAAAGGAAGAT
CTTGCGGCCACGCATTTTCCTGCCGCGGCACTTGATCTTCTGAGGCAGTTTCTCGCAATCCGCGTTTCGCTCGATACGGC
AGCGGTCACGCTGCGCGCCTTCGCGGCGGATAATGCGCTGGACCTGGGTGCGGTTTTGCAAAAATTCGAGGCGCGCGCCG
ATGCCATTGCGCAGGCAGGCATCGAAATGAAGGACATCATCTATGATGCCTCTTTCGGGCGTCCACTCGATTATTATACC
GGGCTTGTCTATGAAATCCGCGATGCGTCGAACAGGCAGGACGGCGTTCTGGCCGGTGGCGGCCGGTATGATCGGCTTCT
GACCATGCTTGGCGCTTGCGAAGCCATTCCCGGCGTCGGTTTCTCCATCTGGCTTGACCGGCTTCAGGCGCTGGCGGGAG
AAAAGCAATGA

Upstream 100 bases:

>100_bases
CGCAGATTACGGTCCGGGAAGACGGGCTGGTCGACGCTGTCCGCGAAATCCTTGATGCCCAGGCGCGCGACCGCGCCGAA
CAATCGAAGTGATCGTGACC

Downstream 100 bases:

>100_bases
GCGTCACATTGGCATTGCCGTCCAAGGGGCGGCTGAAGGAAAAGACGCTCGCCGTGCTGGAAAAGGCCGGTTACAAGGTG
GTTTTGCCGGATGACGACCG

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MVGSRTSPIFNALRVELNAREAELVEIPLIQPADPFLDMAGEDLRRRIFLTENENGDSLCLRPEFTIPVCRNHIALNAAT
PKRYAYLGEVFRQRRDGAAEFLQAGIEDLGAADEAASDARSLADALSCVKAIAPDAPLEIVLGDQSVFAGMLKALGLPQG
WRKKLLRSFGDAHSMDLALAELTGTQRRDPLPESLAVLVAEGDEIGLARMLEAEMLEAGISPGAGRTPVEIARRLIEKED
LAATHFPAAALDLLRQFLAIRVSLDTAAVTLRAFAADNALDLGAVLQKFEARADAIAQAGIEMKDIIYDASFGRPLDYYT
GLVYEIRDASNRQDGVLAGGGRYDRLLTMLGACEAIPGVGFSIWLDRLQALAGEKQ

Sequences:

>Translated_376_residues
MVGSRTSPIFNALRVELNAREAELVEIPLIQPADPFLDMAGEDLRRRIFLTENENGDSLCLRPEFTIPVCRNHIALNAAT
PKRYAYLGEVFRQRRDGAAEFLQAGIEDLGAADEAASDARSLADALSCVKAIAPDAPLEIVLGDQSVFAGMLKALGLPQG
WRKKLLRSFGDAHSMDLALAELTGTQRRDPLPESLAVLVAEGDEIGLARMLEAEMLEAGISPGAGRTPVEIARRLIEKED
LAATHFPAAALDLLRQFLAIRVSLDTAAVTLRAFAADNALDLGAVLQKFEARADAIAQAGIEMKDIIYDASFGRPLDYYT
GLVYEIRDASNRQDGVLAGGGRYDRLLTMLGACEAIPGVGFSIWLDRLQALAGEKQ
>Mature_376_residues
MVGSRTSPIFNALRVELNAREAELVEIPLIQPADPFLDMAGEDLRRRIFLTENENGDSLCLRPEFTIPVCRNHIALNAAT
PKRYAYLGEVFRQRRDGAAEFLQAGIEDLGAADEAASDARSLADALSCVKAIAPDAPLEIVLGDQSVFAGMLKALGLPQG
WRKKLLRSFGDAHSMDLALAELTGTQRRDPLPESLAVLVAEGDEIGLARMLEAEMLEAGISPGAGRTPVEIARRLIEKED
LAATHFPAAALDLLRQFLAIRVSLDTAAVTLRAFAADNALDLGAVLQKFEARADAIAQAGIEMKDIIYDASFGRPLDYYT
GLVYEIRDASNRQDGVLAGGGRYDRLLTMLGACEAIPGVGFSIWLDRLQALAGEKQ

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 40659; Mature: 40659

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGSRTSPIFNALRVELNAREAELVEIPLIQPADPFLDMAGEDLRRRIFLTENENGDSLC
CCCCCCCHHHHHHHHEECCCCCEEEEECCCCCCCHHHHHCCHHHHHEEEEEECCCCCEEE
LRPEFTIPVCRNHIALNAATPKRYAYLGEVFRQRRDGAAEFLQAGIEDLGAADEAASDAR
ECCCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
SLADALSCVKAIAPDAPLEIVLGDQSVFAGMLKALGLPQGWRKKLLRSFGDAHSMDLALA
HHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHH
ELTGTQRRDPLPESLAVLVAEGDEIGLARMLEAEMLEAGISPGAGRTPVEIARRLIEKED
HHCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LAATHFPAAALDLLRQFLAIRVSLDTAAVTLRAFAADNALDLGAVLQKFEARADAIAQAG
HHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
IEMKDIIYDASFGRPLDYYTGLVYEIRDASNRQDGVLAGGGRYDRLLTMLGACEAIPGVG
CCHHHHHEECCCCCCHHHHHCHHEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCH
FSIWLDRLQALAGEKQ
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVGSRTSPIFNALRVELNAREAELVEIPLIQPADPFLDMAGEDLRRRIFLTENENGDSLC
CCCCCCCHHHHHHHHEECCCCCEEEEECCCCCCCHHHHHCCHHHHHEEEEEECCCCCEEE
LRPEFTIPVCRNHIALNAATPKRYAYLGEVFRQRRDGAAEFLQAGIEDLGAADEAASDAR
ECCCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
SLADALSCVKAIAPDAPLEIVLGDQSVFAGMLKALGLPQGWRKKLLRSFGDAHSMDLALA
HHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHH
ELTGTQRRDPLPESLAVLVAEGDEIGLARMLEAEMLEAGISPGAGRTPVEIARRLIEKED
HHCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LAATHFPAAALDLLRQFLAIRVSLDTAAVTLRAFAADNALDLGAVLQKFEARADAIAQAG
HHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
IEMKDIIYDASFGRPLDYYTGLVYEIRDASNRQDGVLAGGGRYDRLLTMLGACEAIPGVG
CCHHHHHEECCCCCCHHHHHCHHEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCH
FSIWLDRLQALAGEKQ
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA