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The map label for this gene is tag [H]

Identifier: 225686040

GI number: 225686040

Start: 169521

End: 170177

Strand: Direct

Name: tag [H]

Synonym: BMEA_B0185

Alternate gene names: 225686040

Gene position: 169521-170177 (Clockwise)

Preceding gene: 225686037

Following gene: 225686041

Centisome position: 14.3

GC content: 54.95

Gene sequence:

>657_bases
ATGAGCGATATGGCCGAGGCAAGGGCAGGGCTGGTCGTTGGGGCCGATGGCAGGACGCGCTGTTTCTGTCCGGGCGAATT
GCCGGATTATCTCGCCTATCACGATAATGAATGGGGCGTGCCTGTTGCCGATGATTTCCGGCTTTTTGAGAAAATCTGTC
TTGAAGGGTTCCAGTCGGGATTGTCGTGGCTTACGATTTTGCGCAAGCGCGAAAATTTCCGCGCGGCCTTTGATGGTTTC
AATTTTCATCGCATTGCCACCTATGACGAAAGCGATGTGGAACGCCTTCTGGCGGACAAGGGCATCGTGCGCCATCGCGG
CAAGATCGTTTCGGTCATCAACAATGCCGGGCGCGCCATCGAGCTGATCGAGGAGAAGGGGTCGCTTGCCGCCTATTTCT
GGTCGTTCGAGCCGGGCGCGGAAAACAGGCCCGCAGTGGTCGATTATCCCACGCTTGTCGCAAACCCCATAACCGCAACC
TCGACCCGCATTTCAAAAGACCTGAAAAAACGCGGCTGGTCTTTCGTCGGTCCAACCACGGTCTATGCCTTCATGCAGGC
GATGGGCCTCGTGAACGATCATATCGAGGGTTGCCATTGCCGTGCAACGATCGAAAAATTACGACAGAATTTCAAAAGGC
CGGGCAAAAAGCTTTAA

Upstream 100 bases:

>100_bases
TTTCGGCGCGTGTTTCGTCTTGAGGGCCACGCAATTGAACTTGTGGTTAGTGTCGGGTTGCGCAACATGATTAAGGATGA
ATGAAGCGGAGAGCCGGGAA

Downstream 100 bases:

>100_bases
TTGCCAGCCGATGCGGTTTCTGCCAGAAACGCATCTGGATGCATTCACGCACGTCTTTATTCCACAGCCGGTTCCATTTA
TGGGAAACGCGCTCTGGTAA

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSDMAEARAGLVVGADGRTRCFCPGELPDYLAYHDNEWGVPVADDFRLFEKICLEGFQSGLSWLTILRKRENFRAAFDGF
NFHRIATYDESDVERLLADKGIVRHRGKIVSVINNAGRAIELIEEKGSLAAYFWSFEPGAENRPAVVDYPTLVANPITAT
STRISKDLKKRGWSFVGPTTVYAFMQAMGLVNDHIEGCHCRATIEKLRQNFKRPGKKL

Sequences:

>Translated_218_residues
MSDMAEARAGLVVGADGRTRCFCPGELPDYLAYHDNEWGVPVADDFRLFEKICLEGFQSGLSWLTILRKRENFRAAFDGF
NFHRIATYDESDVERLLADKGIVRHRGKIVSVINNAGRAIELIEEKGSLAAYFWSFEPGAENRPAVVDYPTLVANPITAT
STRISKDLKKRGWSFVGPTTVYAFMQAMGLVNDHIEGCHCRATIEKLRQNFKRPGKKL
>Mature_217_residues
SDMAEARAGLVVGADGRTRCFCPGELPDYLAYHDNEWGVPVADDFRLFEKICLEGFQSGLSWLTILRKRENFRAAFDGFN
FHRIATYDESDVERLLADKGIVRHRGKIVSVINNAGRAIELIEEKGSLAAYFWSFEPGAENRPAVVDYPTLVANPITATS
TRISKDLKKRGWSFVGPTTVYAFMQAMGLVNDHIEGCHCRATIEKLRQNFKRPGKKL

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=182, Percent_Identity=50.5494505494505, Blast_Score=186, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 24520; Mature: 24389

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMAEARAGLVVGADGRTRCFCPGELPDYLAYHDNEWGVPVADDFRLFEKICLEGFQSG
CCCHHHHHCCEEECCCCCEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LSWLTILRKRENFRAAFDGFNFHRIATYDESDVERLLADKGIVRHRGKIVSVINNAGRAI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHCCCHHH
ELIEEKGSLAAYFWSFEPGAENRPAVVDYPTLVANPITATSTRISKDLKKRGWSFVGPTT
HHHHCCCCEEEEEEEECCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCCCHHH
VYAFMQAMGLVNDHIEGCHCRATIEKLRQNFKRPGKKL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDMAEARAGLVVGADGRTRCFCPGELPDYLAYHDNEWGVPVADDFRLFEKICLEGFQSG
CCHHHHHCCEEECCCCCEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LSWLTILRKRENFRAAFDGFNFHRIATYDESDVERLLADKGIVRHRGKIVSVINNAGRAI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHCCCHHH
ELIEEKGSLAAYFWSFEPGAENRPAVVDYPTLVANPITATSTRISKDLKKRGWSFVGPTT
HHHHCCCCEEEEEEEECCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCCCHHH
VYAFMQAMGLVNDHIEGCHCRATIEKLRQNFKRPGKKL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]