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The map label for this gene is ywbI [H]

Identifier: 225686021

GI number: 225686021

Start: 149892

End: 150776

Strand: Reverse

Name: ywbI [H]

Synonym: BMEA_B0164

Alternate gene names: 225686021

Gene position: 150776-149892 (Counterclockwise)

Preceding gene: 225686030

Following gene: 225686007

Centisome position: 12.72

GC content: 57.4

Gene sequence:

>885_bases
ATGCGTTTTGATGTCACTGATTTAAGGTTGTTTGTCAGCATCGCAGAAACCGGCAGCATCACGCATGGCGCCGCCATGGT
GAACATGGCGTTGGGCGCGGCCAGCGCCAGGGTCCGCGGCATGGAGGAATTGGCAGGGGTAGCCCTGCTCAATCGCGAAC
GCCTGGGCGTATCCATTACACCTGCGGGGCGCGCGCTGCTACAGCATGGACGCATTGTCCTGCAACAACTCGGGCGGATG
CATAGTGAGTTGGACGAGTTTGCCCATGGTCTCAAGGGACATGTGCGGCTCTTTTCCAACACCAATGCCCTTGCTGAATT
TTTGCCGGTCGCGCTCAGTTCATTTCTGATGGACAATCCCGGCGTGAACATCGATCTACAGGAACATCTAAGCGATGAGA
TCGTCAGAAGCGTTGTGGAGGGCATTGCCGATATCGGCATTGTGGCTGGCACTGTGGATGTTGCTTCTCTGGAAACATTT
CCATTTCGGGAAGATTATCTGGTCGTCGTGGTCCCGCAGGGGCACCCGTTTGCGCGTCGCCGGCGCATCGCGTTCAAAGA
TACGCTCGATTATGATTTCATCGGCCTTTCACAGGGGGCAGCCCTGCAAAATTTCCTGTCGGCCCATGCGATGCGGATGG
GACAGCGCCTGCGCCTGCGTGTTCAGCTTCGCAGCTTCGACAGCATTTGCCAGATGGTCGAAAGCCGGGCCGGAATTGCA
ATCGTACCGGCCTCAGCCGCAAAGCGCCTCCGGCGCATCATGGCAATCCGTATAATCGCGCTTGACGATGCCTGGGCATT
GCGGCGGCTGACCATATGCGTGCGTTCATTGCAAGACCTTCCCGCCCATGCGGCTCGATTGGTGGAGCATCTTCGGCAAA
CTTGA

Upstream 100 bases:

>100_bases
TTCCATTTGACGAATTAAACCTTGACCGCGTGCGGATGACCATGCGCATTTTTAAAAGGAAGCCTTCGCTAAAATTGAAG
GCTGGTCCAGGAGACTGTCC

Downstream 100 bases:

>100_bases
AGCTAAAGCATGTCTCCGAAAAGCGGATGACCCTATCATTGTCGAAGGTCCATTTGCCCCGAACTCCACCTATCTTGACG
CTCTGACAAACTTGACCGGA

Product: putative transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MRFDVTDLRLFVSIAETGSITHGAAMVNMALGAASARVRGMEELAGVALLNRERLGVSITPAGRALLQHGRIVLQQLGRM
HSELDEFAHGLKGHVRLFSNTNALAEFLPVALSSFLMDNPGVNIDLQEHLSDEIVRSVVEGIADIGIVAGTVDVASLETF
PFREDYLVVVVPQGHPFARRRRIAFKDTLDYDFIGLSQGAALQNFLSAHAMRMGQRLRLRVQLRSFDSICQMVESRAGIA
IVPASAAKRLRRIMAIRIIALDDAWALRRLTICVRSLQDLPAHAARLVEHLRQT

Sequences:

>Translated_294_residues
MRFDVTDLRLFVSIAETGSITHGAAMVNMALGAASARVRGMEELAGVALLNRERLGVSITPAGRALLQHGRIVLQQLGRM
HSELDEFAHGLKGHVRLFSNTNALAEFLPVALSSFLMDNPGVNIDLQEHLSDEIVRSVVEGIADIGIVAGTVDVASLETF
PFREDYLVVVVPQGHPFARRRRIAFKDTLDYDFIGLSQGAALQNFLSAHAMRMGQRLRLRVQLRSFDSICQMVESRAGIA
IVPASAAKRLRRIMAIRIIALDDAWALRRLTICVRSLQDLPAHAARLVEHLRQT
>Mature_294_residues
MRFDVTDLRLFVSIAETGSITHGAAMVNMALGAASARVRGMEELAGVALLNRERLGVSITPAGRALLQHGRIVLQQLGRM
HSELDEFAHGLKGHVRLFSNTNALAEFLPVALSSFLMDNPGVNIDLQEHLSDEIVRSVVEGIADIGIVAGTVDVASLETF
PFREDYLVVVVPQGHPFARRRRIAFKDTLDYDFIGLSQGAALQNFLSAHAMRMGQRLRLRVQLRSFDSICQMVESRAGIA
IVPASAAKRLRRIMAIRIIALDDAWALRRLTICVRSLQDLPAHAARLVEHLRQT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=246, Percent_Identity=23.1707317073171, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI157672245, Length=251, Percent_Identity=23.9043824701195, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786401, Length=242, Percent_Identity=24.7933884297521, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787879, Length=267, Percent_Identity=23.9700374531835, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787806, Length=275, Percent_Identity=25.0909090909091, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788481, Length=179, Percent_Identity=24.5810055865922, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787601, Length=201, Percent_Identity=26.3681592039801, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=22.7450980392157, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788887, Length=181, Percent_Identity=26.5193370165746, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32416; Mature: 32416

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDVTDLRLFVSIAETGSITHGAAMVNMALGAASARVRGMEELAGVALLNRERLGVSIT
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
PAGRALLQHGRIVLQQLGRMHSELDEFAHGLKGHVRLFSNTNALAEFLPVALSSFLMDNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCC
GVNIDLQEHLSDEIVRSVVEGIADIGIVAGTVDVASLETFPFREDYLVVVVPQGHPFARR
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCCCCCCCEEEEEEECCCCHHHH
RRIAFKDTLDYDFIGLSQGAALQNFLSAHAMRMGQRLRLRVQLRSFDSICQMVESRAGIA
HCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCEE
IVPASAAKRLRRIMAIRIIALDDAWALRRLTICVRSLQDLPAHAARLVEHLRQT
EECHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRFDVTDLRLFVSIAETGSITHGAAMVNMALGAASARVRGMEELAGVALLNRERLGVSIT
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
PAGRALLQHGRIVLQQLGRMHSELDEFAHGLKGHVRLFSNTNALAEFLPVALSSFLMDNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCC
GVNIDLQEHLSDEIVRSVVEGIADIGIVAGTVDVASLETFPFREDYLVVVVPQGHPFARR
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCCCCCCCEEEEEEECCCCHHHH
RRIAFKDTLDYDFIGLSQGAALQNFLSAHAMRMGQRLRLRVQLRSFDSICQMVESRAGIA
HCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCEE
IVPASAAKRLRRIMAIRIIALDDAWALRRLTICVRSLQDLPAHAARLVEHLRQT
EECHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]