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The map label for this gene is rfbB [C]

Identifier: 225686003

GI number: 225686003

Start: 137307

End: 138272

Strand: Reverse

Name: rfbB [C]

Synonym: BMEA_B0146

Alternate gene names: 225686003

Gene position: 138272-137307 (Counterclockwise)

Preceding gene: 225686004

Following gene: 225686002

Centisome position: 11.66

GC content: 60.14

Gene sequence:

>966_bases
ATGGCTAAAATCGTCGTTACCGGGGGCGCCGGCTTCCTGGGCAGTCGTTTGATCCGTGGGCTTCTGGCATCGCGGGGACA
AAACGGGGTTCCCTCTTTCGACAGCATCGTTTCTGTCGATCTCGTGGCGTGCTCCATCGATGATCCACGCATCACGTCGG
TTACGGGCGATATTGCCGATCCGGCATTTGCCCGTTCAGTCATCACAAAAGGGACGGTTGGTGTCTATCACATGGCCGCC
GCGCTCAGCGGTCAATCCGAAGCCGAATTCGACGTCGGCATGCGTGTGAACATTGACGGAACCCGCGCCCTGCTGGAAGC
CGCGCGGGCAACGCAGGAAGCACCGAAGTTTATCTTCACCTCCTCGCTTGCCGTGTTTGGCGGGGAAATGCCGGATGTCG
TGCCGGAAAACCTGGCGCTGTTGCCGCAATCGTCCTATGGGGCGGAAAAGGCAATCGGGGAGTTTCTGGTTGGCGATTAT
TCCCGCAGAGGCTTCATCGATGGCCGTATCTGCCGCCTGCCCACGATCGTCGTGCGCCCTGGCAAGCCGAATTCTGCGGC
ATCCTCCTTTGCAAGCGGCATAATCCGCGAGCCGCTGGCAGGCATCGCCTCCAACAATCCGGTGCCACCCGCAACGCGCA
TGTGGCTTTCCTCGCCGGATATTGCTGCCCGCAACCTCGTTCACGCAATCACTGTCGAAGGTTCGGCCCTCGGTATCAAT
CGCGTGCTCAACCTGCCGGGGATCTGCGTTACCGTCGCCGATATGCTGGACAGCCTTGAGCGCATTGCCGGAAAGGACGT
GCGGGCGCTGGTTTCAGCCGAACCGGAACAGCGCGTCATCGATATTGTCTGCTCATGGCCCGGCGAATTCGACATCGCTC
GACCGCTGGCGCTTGGTTTTACACGCGATGCCGATTTCGACGCGGTCGTGCGCCAATATCGGGACGAGTTTGCACACTCT
TCCTGA

Upstream 100 bases:

>100_bases
ATGCGGGCCTGCCCGAAAATGCGCGGGAAACAATCACGCAGCGCGGCGTCGAGTTGCATCTGGTTGAAGCCGGCACAGGC
AAAGTCGAAGGGAAAATCTG

Downstream 100 bases:

>100_bases
TGGCCACAAATTTGGAACAAAGAACATGACAGAACAAGAAATTCGCGAGCTTATCGTCGAGCTTGGTGCCAGCCTTTTTG
CTCGCGGATATTGCGTGGGC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MAKIVVTGGAGFLGSRLIRGLLASRGQNGVPSFDSIVSVDLVACSIDDPRITSVTGDIADPAFARSVITKGTVGVYHMAA
ALSGQSEAEFDVGMRVNIDGTRALLEAARATQEAPKFIFTSSLAVFGGEMPDVVPENLALLPQSSYGAEKAIGEFLVGDY
SRRGFIDGRICRLPTIVVRPGKPNSAASSFASGIIREPLAGIASNNPVPPATRMWLSSPDIAARNLVHAITVEGSALGIN
RVLNLPGICVTVADMLDSLERIAGKDVRALVSAEPEQRVIDIVCSWPGEFDIARPLALGFTRDADFDAVVRQYRDEFAHS
S

Sequences:

>Translated_321_residues
MAKIVVTGGAGFLGSRLIRGLLASRGQNGVPSFDSIVSVDLVACSIDDPRITSVTGDIADPAFARSVITKGTVGVYHMAA
ALSGQSEAEFDVGMRVNIDGTRALLEAARATQEAPKFIFTSSLAVFGGEMPDVVPENLALLPQSSYGAEKAIGEFLVGDY
SRRGFIDGRICRLPTIVVRPGKPNSAASSFASGIIREPLAGIASNNPVPPATRMWLSSPDIAARNLVHAITVEGSALGIN
RVLNLPGICVTVADMLDSLERIAGKDVRALVSAEPEQRVIDIVCSWPGEFDIARPLALGFTRDADFDAVVRQYRDEFAHS
S
>Mature_320_residues
AKIVVTGGAGFLGSRLIRGLLASRGQNGVPSFDSIVSVDLVACSIDDPRITSVTGDIADPAFARSVITKGTVGVYHMAAA
LSGQSEAEFDVGMRVNIDGTRALLEAARATQEAPKFIFTSSLAVFGGEMPDVVPENLALLPQSSYGAEKAIGEFLVGDYS
RRGFIDGRICRLPTIVVRPGKPNSAASSFASGIIREPLAGIASNNPVPPATRMWLSSPDIAARNLVHAITVEGSALGINR
VLNLPGICVTVADMLDSLERIAGKDVRALVSAEPEQRVIDIVCSWPGEFDIARPLALGFTRDADFDAVVRQYRDEFAHSS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Drosophila melanogaster, GI24667531, Length=293, Percent_Identity=26.962457337884, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 33925; Mature: 33794

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVVTGGAGFLGSRLIRGLLASRGQNGVPSFDSIVSVDLVACSIDDPRITSVTGDIAD
CCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEEEECCCCCEEEECCCCCC
PAFARSVITKGTVGVYHMAAALSGQSEAEFDVGMRVNIDGTRALLEAARATQEAPKFIFT
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHH
SSLAVFGGEMPDVVPENLALLPQSSYGAEKAIGEFLVGDYSRRGFIDGRICRLPTIVVRP
HHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCEEEEEC
GKPNSAASSFASGIIREPLAGIASNNPVPPATRMWLSSPDIAARNLVHAITVEGSALGIN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHEEEEEECCCHHHHH
RVLNLPGICVTVADMLDSLERIAGKDVRALVSAEPEQRVIDIVCSWPGEFDIARPLALGF
HHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHEEEEEEECCCCCCCCCCHHHHCC
TRDADFDAVVRQYRDEFAHSS
CCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKIVVTGGAGFLGSRLIRGLLASRGQNGVPSFDSIVSVDLVACSIDDPRITSVTGDIAD
CEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEEEECCCCCEEEECCCCCC
PAFARSVITKGTVGVYHMAAALSGQSEAEFDVGMRVNIDGTRALLEAARATQEAPKFIFT
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCHHHH
SSLAVFGGEMPDVVPENLALLPQSSYGAEKAIGEFLVGDYSRRGFIDGRICRLPTIVVRP
HHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCEEEEEC
GKPNSAASSFASGIIREPLAGIASNNPVPPATRMWLSSPDIAARNLVHAITVEGSALGIN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHEEEEEECCCHHHHH
RVLNLPGICVTVADMLDSLERIAGKDVRALVSAEPEQRVIDIVCSWPGEFDIARPLALGF
HHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHEEEEEEECCCCCCCCCCHHHHCC
TRDADFDAVVRQYRDEFAHSS
CCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]