Click here to switch to the map view.

The map label for this gene is virB8

Identifier: 225685929

GI number: 225685929

Start: 59908

End: 60627

Strand: Reverse

Name: virB8

Synonym: BMEA_B0066

Alternate gene names: 225685929

Gene position: 60627-59908 (Counterclockwise)

Preceding gene: 225685930

Following gene: 225685928

Centisome position: 5.11

GC content: 55.69

Gene sequence:

>720_bases
ATGTTTGGACGCAAACAATCTCCACAGAAATCAGTAAAGAACGGGCAGGGCAATGCGCCTTCGGTATATGACGAAGCGTT
GAACTGGGAGGCCGCGCACGTCCGGCTGGTCGAAAAATCCGAACGCCGCGCGTGGAAAATCGCCGGGGCTTTCGGCACCA
TTACCGTGCTGCTCGGGATCGGCATCGCCGGGATGTTACCGCTAAAGCAACATGTGCCCTACCTGGTGCGCGTCAACGCA
CAGACAGGTGCGCCTGATATCCTGACCTCGCTCGACGAGAAGAGTGTCAGCTACGACACTGTAATGGATAAATACTGGCT
CTCGCAGTACGTGATAGCGCGTGAAACCTACGACTGGTACACGCTGCAAAAGGACTACGAAACCGTAGGCATGTTGTCGT
CGCCGTCCGAAGGGCAGAGCTATGCAAGCCAGTTCCAGGGCGATAAGGCGCTTGACAAGCAATATGGCTCAAATGTGCGC
ACATCGGTAACGATCGTAAGCATCGTGCCGAACGGCAAGGGCATCGGCACGGTGCGCTTTGCGAAGACGACAAAGCGCAC
CAACGAGACCGGTGATGGCGAAACAACGCACTGGATCGCCACCATTGGCTACCAATACGTCAATCCCTCTTTGATGTCGG
AATCGGCCCGCCTGACAAACCCGCTGGGTTTCAATGTCACGAGCTATCGCGTTGATCCAGAAATGGGAGTGGTGCAATGA

Upstream 100 bases:

>100_bases
CGGTTCTCGATGGCAAACCGCGGGTTCCCGTCAACAAAAGCGTTCCGGCTAAACCGCCCCTGGCTCAGCCTAACCCCGTT
GACACTTACGAGGACTAATC

Downstream 100 bases:

>100_bases
AAAGATTCCTGCTTGCGTGCATACTGATCACGCTGGCCTCGCCCTCCTGGGCAACCAAAATCCCGTCCGGCTCAAAATAC
GACAGCCGAATTCAGTATGT

Product: type IV secretion system protein virB8

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MFGRKQSPQKSVKNGQGNAPSVYDEALNWEAAHVRLVEKSERRAWKIAGAFGTITVLLGIGIAGMLPLKQHVPYLVRVNA
QTGAPDILTSLDEKSVSYDTVMDKYWLSQYVIARETYDWYTLQKDYETVGMLSSPSEGQSYASQFQGDKALDKQYGSNVR
TSVTIVSIVPNGKGIGTVRFAKTTKRTNETGDGETTHWIATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMGVVQ

Sequences:

>Translated_239_residues
MFGRKQSPQKSVKNGQGNAPSVYDEALNWEAAHVRLVEKSERRAWKIAGAFGTITVLLGIGIAGMLPLKQHVPYLVRVNA
QTGAPDILTSLDEKSVSYDTVMDKYWLSQYVIARETYDWYTLQKDYETVGMLSSPSEGQSYASQFQGDKALDKQYGSNVR
TSVTIVSIVPNGKGIGTVRFAKTTKRTNETGDGETTHWIATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMGVVQ
>Mature_239_residues
MFGRKQSPQKSVKNGQGNAPSVYDEALNWEAAHVRLVEKSERRAWKIAGAFGTITVLLGIGIAGMLPLKQHVPYLVRVNA
QTGAPDILTSLDEKSVSYDTVMDKYWLSQYVIARETYDWYTLQKDYETVGMLSSPSEGQSYASQFQGDKALDKQYGSNVR
TSVTIVSIVPNGKGIGTVRFAKTTKRTNETGDGETTHWIATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMGVVQ

Specific function: The virB operon is essential for intracellular survival and is not involved in the invasion process. Constitutes a major determinant of virulence in mice

COG id: COG3736

COG function: function code U; Type IV secretory pathway, component VirB8

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virB8 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VIRB8_BRUA2 (Q2YJ78)

Other databases:

- EMBL:   AF226278
- EMBL:   AM040265
- RefSeq:   YP_418306.1
- ProteinModelPortal:   Q2YJ78
- SMR:   Q2YJ78
- STRING:   Q2YJ78
- GeneID:   3827898
- GenomeReviews:   AM040265_GR
- KEGG:   bmf:BAB2_0061
- eggNOG:   COG3736
- HOGENOM:   HBG700868
- OMA:   LEMSDES
- ProtClustDB:   CLSK884263
- BioCyc:   BMEL359391:BAB2_0061-MONOMER
- GO:   GO:0009405
- InterPro:   IPR007430

Pfam domain/function: PF04335 VirB8

EC number: NA

Molecular weight: Translated: 26446; Mature: 26446

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1f8f2c78)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRKQSPQKSVKNGQGNAPSVYDEALNWEAAHVRLVEKSERRAWKIAGAFGTITVLLGI
CCCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHEEEEEECCCCCEEEEECHHHHHHHHHHH
GIAGMLPLKQHVPYLVRVNAQTGAPDILTSLDEKSVSYDTVMDKYWLSQYVIARETYDWY
HHHHCCCHHHCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
TLQKDYETVGMLSSPSEGQSYASQFQGDKALDKQYGSNVRTSVTIVSIVPNGKGIGTVRF
EECCCHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEE
AKTTKRTNETGDGETTHWIATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMGVVQ
EEHHCCCCCCCCCCCEEEEEEECHHHCCHHHHCCCHHHCCCCCCCEEEEEECCCCCCCH
>Mature Secondary Structure
MFGRKQSPQKSVKNGQGNAPSVYDEALNWEAAHVRLVEKSERRAWKIAGAFGTITVLLGI
CCCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHEEEEEECCCCCEEEEECHHHHHHHHHHH
GIAGMLPLKQHVPYLVRVNAQTGAPDILTSLDEKSVSYDTVMDKYWLSQYVIARETYDWY
HHHHCCCHHHCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
TLQKDYETVGMLSSPSEGQSYASQFQGDKALDKQYGSNVRTSVTIVSIVPNGKGIGTVRF
EECCCHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEE
AKTTKRTNETGDGETTHWIATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMGVVQ
EEHHCCCCCCCCCCCEEEEEEECHHHCCHHHHCCCHHHCCCCCCCEEEEEECCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10940027