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The map label for this gene is pdhC [H]

Identifier: 225685900

GI number: 225685900

Start: 30192

End: 31457

Strand: Direct

Name: pdhC [H]

Synonym: BMEA_B0035

Alternate gene names: 225685900

Gene position: 30192-31457 (Clockwise)

Preceding gene: 225685899

Following gene: 225685901

Centisome position: 2.55

GC content: 61.61

Gene sequence:

>1266_bases
ATGCCGGTTGAAGTCGTATTGCCTGCCCTTTCGGCAGGAATGGAAGATGCAGTCATCGCGCGCTGGCTCAAGGCCGAAGG
CGACGCGGTGAGCAAGGGCGACCTGATCGCCGAAGTCGAAACCGACAAGGCGACGATGGAGCTTGAAGCCGAAGTGGATG
GCCGCATTGGCCAGCTTCTGGTGAAGGACGGTGCGCGCGCCAATGTCAATCAGGTGATCGCCCTGCTTTTGAAGGAAGGC
GAAGATGCTTCTGCAATGGCCGGATTTGCGGTCGGCTCCAGTCCTGTGGCCGTGGCAGAAGCCGAAACCCCTGTTGCAGC
CTCGCCGGTGCCAGCCGCACCAGCAGTAAGCGCGCCCGCCTCCGGTGAAGTGCGCCACAAGGCTTCGCCGCTCGCCCGCC
GCCTGGCTGCGGAACTGGGCGTTTCGCTGGACGGGCTTGCGGGAAGTGGCGCAAGGGGCCGTATCGTACGGATCGACGTG
GAACGCGCGGCCGCCTCGAAGCCGGTTCCGGTTGCCGCCGCTGCCGCTCCAGTCGCCGCACCTGCGGAAGTTTCGTCGAA
AGCCATTCCGGTTGGCATCGGCGAGTATGAAGCCGTTCCGCACACATCCATGCGCCGCACCATTGCCCGCCGCCTGCTGG
AAGCCAAGACAACGGTGCCGCATTTCTATCTGAACGTGGATTGCGAGATCGATGCGCTTCTGGCATTGCGCAGCCAGATC
AACGAGAAGCGGGAAGGTTCGGCCCGTATTTCCGTCAATGATTTTGTCATCAAGGCTTCCGCCGCCGCCCTGCGCCGGGT
GCCCGATGCCAATGTCATATGGACAGACGAGGCGCTTTTGAAGCTGAAGGACGTCGATATTGCCGTTGCGGTGGCGACGG
AAGGCGGCCTTATCACACCGATTATTCGCAGTGCCGACCAGATGAGCCTTGGCGCTATTTCGGCGCAGATGAAGTCTCTG
GCCGCGCGTGCCCGCGAAAACCGCCTGAAGCCGGAAGAATTCCAGGGCGGTGGCTTTTCGATTTCCAATCTCAGCATGTA
TGGTGTCAAATCCTTTTCGGCCATCATCAATCCGCCGCAAAGCGCCATTCTGGCGGTTGGCGCGGGCGAACGCCGCCCAA
TCGAACGCAATGGTGAGCTTGCTTTCGCCACCATGATGAGCGTGACGCTTTCGGTTGACCATCGCGCGGTCGACGGCGCC
CTGGGTGCGCAACTGCTCGCGGCCTTCAAGGCCGGTATTGAAGATCCGATGTCTCTGCTTGTCTAG

Upstream 100 bases:

>100_bases
GGCGGCTGCCGCGCGCCCACAGGACATTGAGGCTGGCCTGCGCGCCGTCATGGCTGCCCAGGGCCTGCCCATCGAGAATT
GATGCACAAGGAGACAGACA

Downstream 100 bases:

>100_bases
ATCGGTTCCAGTTGGAGCCGATCTATCTCTCTGTTTTTAAATATGTCTTTATCCCAAAAGCGGTTTCCGTTTTTGGGAGA
CATGCCCCAGGAGCGATGCA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPVEVVLPALSAGMEDAVIARWLKAEGDAVSKGDLIAEVETDKATMELEAEVDGRIGQLLVKDGARANVNQVIALLLKEG
EDASAMAGFAVGSSPVAVAEAETPVAASPVPAAPAVSAPASGEVRHKASPLARRLAAELGVSLDGLAGSGARGRIVRIDV
ERAAASKPVPVAAAAAPVAAPAEVSSKAIPVGIGEYEAVPHTSMRRTIARRLLEAKTTVPHFYLNVDCEIDALLALRSQI
NEKREGSARISVNDFVIKASAAALRRVPDANVIWTDEALLKLKDVDIAVAVATEGGLITPIIRSADQMSLGAISAQMKSL
AARARENRLKPEEFQGGGFSISNLSMYGVKSFSAIINPPQSAILAVGAGERRPIERNGELAFATMMSVTLSVDHRAVDGA
LGAQLLAAFKAGIEDPMSLLV

Sequences:

>Translated_421_residues
MPVEVVLPALSAGMEDAVIARWLKAEGDAVSKGDLIAEVETDKATMELEAEVDGRIGQLLVKDGARANVNQVIALLLKEG
EDASAMAGFAVGSSPVAVAEAETPVAASPVPAAPAVSAPASGEVRHKASPLARRLAAELGVSLDGLAGSGARGRIVRIDV
ERAAASKPVPVAAAAAPVAAPAEVSSKAIPVGIGEYEAVPHTSMRRTIARRLLEAKTTVPHFYLNVDCEIDALLALRSQI
NEKREGSARISVNDFVIKASAAALRRVPDANVIWTDEALLKLKDVDIAVAVATEGGLITPIIRSADQMSLGAISAQMKSL
AARARENRLKPEEFQGGGFSISNLSMYGVKSFSAIINPPQSAILAVGAGERRPIERNGELAFATMMSVTLSVDHRAVDGA
LGAQLLAAFKAGIEDPMSLLV
>Mature_420_residues
PVEVVLPALSAGMEDAVIARWLKAEGDAVSKGDLIAEVETDKATMELEAEVDGRIGQLLVKDGARANVNQVIALLLKEGE
DASAMAGFAVGSSPVAVAEAETPVAASPVPAAPAVSAPASGEVRHKASPLARRLAAELGVSLDGLAGSGARGRIVRIDVE
RAAASKPVPVAAAAAPVAAPAEVSSKAIPVGIGEYEAVPHTSMRRTIARRLLEAKTTVPHFYLNVDCEIDALLALRSQIN
EKREGSARISVNDFVIKASAAALRRVPDANVIWTDEALLKLKDVDIAVAVATEGGLITPIIRSADQMSLGAISAQMKSLA
ARARENRLKPEEFQGGGFSISNLSMYGVKSFSAIINPPQSAILAVGAGERRPIERNGELAFATMMSVTLSVDHRAVDGAL
GAQLLAAFKAGIEDPMSLLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=440, Percent_Identity=41.3636363636364, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=37.0044052863436, Blast_Score=275, Evalue=8e-74,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=37.0044052863436, Blast_Score=275, Evalue=9e-74,
Organism=Homo sapiens, GI260898739, Length=165, Percent_Identity=44.8484848484849, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI110671329, Length=437, Percent_Identity=25.629290617849, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI19923748, Length=222, Percent_Identity=34.6846846846847, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1786946, Length=424, Percent_Identity=32.311320754717, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1786305, Length=405, Percent_Identity=30.8641975308642, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=444, Percent_Identity=41.2162162162162, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=39.9350649350649, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17537937, Length=440, Percent_Identity=29.7727272727273, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=423, Percent_Identity=29.7872340425532, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=37.0535714285714, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6320352, Length=430, Percent_Identity=25.5813953488372, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI20129315, Length=444, Percent_Identity=40.5405405405405, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24582497, Length=433, Percent_Identity=40.1847575057737, Blast_Score=277, Evalue=8e-75,
Organism=Drosophila melanogaster, GI18859875, Length=430, Percent_Identity=26.7441860465116, Blast_Score=141, Evalue=8e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43688; Mature: 43556

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVEVVLPALSAGMEDAVIARWLKAEGDAVSKGDLIAEVETDKATMELEAEVDGRIGQLL
CCCEEEEHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHE
VKDGARANVNQVIALLLKEGEDASAMAGFAVGSSPVAVAEAETPVAASPVPAAPAVSAPA
ECCCCCCCHHHHHHHHHHCCCCCHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCC
SGEVRHKASPLARRLAAELGVSLDGLAGSGARGRIVRIDVERAAASKPVPVAAAAAPVAA
CCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCEEEEEEEHHHCCCCCCCEECCCCCCCC
PAEVSSKAIPVGIGEYEAVPHTSMRRTIARRLLEAKTTVPHFYLNVDCEIDALLALRSQI
CHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
NEKREGSARISVNDFVIKASAAALRRVPDANVIWTDEALLKLKDVDIAVAVATEGGLITP
HHHHCCCEEEEEEHEEEEHHHHHHHCCCCCCEEECCHHHEEEECCEEEEEEECCCCEEEH
IIRSADQMSLGAISAQMKSLAARARENRLKPEEFQGGGFSISNLSMYGVKSFSAIINPPQ
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEECCEEEECHHHHHHHCCCCC
SAILAVGAGERRPIERNGELAFATMMSVTLSVDHRAVDGALGAQLLAAFKAGIEDPMSLL
CEEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHC
V
C
>Mature Secondary Structure 
PVEVVLPALSAGMEDAVIARWLKAEGDAVSKGDLIAEVETDKATMELEAEVDGRIGQLL
CCEEEEHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHE
VKDGARANVNQVIALLLKEGEDASAMAGFAVGSSPVAVAEAETPVAASPVPAAPAVSAPA
ECCCCCCCHHHHHHHHHHCCCCCHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCC
SGEVRHKASPLARRLAAELGVSLDGLAGSGARGRIVRIDVERAAASKPVPVAAAAAPVAA
CCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCEEEEEEEHHHCCCCCCCEECCCCCCCC
PAEVSSKAIPVGIGEYEAVPHTSMRRTIARRLLEAKTTVPHFYLNVDCEIDALLALRSQI
CHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
NEKREGSARISVNDFVIKASAAALRRVPDANVIWTDEALLKLKDVDIAVAVATEGGLITP
HHHHCCCEEEEEEHEEEEHHHHHHHCCCCCCEEECCHHHEEEECCEEEEEEECCCCEEEH
IIRSADQMSLGAISAQMKSLAARARENRLKPEEFQGGGFSISNLSMYGVKSFSAIINPPQ
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCEEECCEEEECHHHHHHHCCCCC
SAILAVGAGERRPIERNGELAFATMMSVTLSVDHRAVDGALGAQLLAAFKAGIEDPMSLL
CEEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]