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The map label for this gene is yfiY [H]

Identifier: 222527348

GI number: 222527348

Start: 5121795

End: 5122952

Strand: Direct

Name: yfiY [H]

Synonym: Chy400_4136

Alternate gene names: 222527348

Gene position: 5121795-5122952 (Clockwise)

Preceding gene: 222527347

Following gene: 222527349

Centisome position: 97.21

GC content: 57.51

Gene sequence:

>1158_bases
ATGTGTGCTGCAACGCTCATTGACACTACCGACGCTCTGCTCAGTCGCTATGATCAGCTCGACCTGCGTGGCCTGAGCAT
CACTGAAATCGCTGAGTTGCTGCGCCAGCAGGGCGCAGCGCACCTCAGCCCGATTGAACGCATCGAGCTGGCCGAGCGGC
TCATTACCCGCCGTCGCTTCTTGATCGGTGCAGGGGCGTTAGCTCTGGGCATAATCACCGGCTGTGGGACGCAGGAGCAG
GCTGCCGCACCAACGGCGATGGTGGCAACAACGCGCACGGTTGTCGACGGCCTGGGGCGAAGGGTCGAGGTGCCAGCCGT
GCCGCAGCGCGTGGTGGCTTTACACGATCTGGACAACGCCTATGCACTGGCATCGCTAGGCTTTGCTCCGATTGGCATGG
GCAGCATTACACTCGGTGATCCCTTCGAGCGGTTACGATCCCTCGGCCCTATCCCGCCAGCACTGGAGCAGACGGTTGAG
GTTGGGTTATTGTACGAGCCGAACCTTGAAGCAATCGCTTCCTTACAACCTGATCTCATCCTGGGCACACGTGGCTCACA
CCAACAGATCCTCGATCAACTGACAGCAATCGCGCCGACTGTCCTGATCGATCAGACTGCTGGCGACGATGTGCTATCGA
ACCAGCGCTTTCTGGCATCGCTCGTCGGCGTGGAGGATCGGATCGACGAGCAGCTTGCCCGCTACGAAGCGAGGATCGCA
GCACTCAGAGAACGCTATGCTGATACGCTAAACACCCTCGAATATACCCGCATCGACAGCTACGGTGTCGGTGCTGAAGA
TAACTACCTGATCCTGTTCGATCTGACCCCTGGCACCCGGGTGTTGACCGATCTTGGCGCACGACGATCAAAAACGAATG
GCAACGTTGACAATGACCCGTTTCCCGCGATTAGCCTGGAGCGCCTGGCCGACTACGATGCCGATGTCATCTTCATCGGC
GTTGAGGCCGGTACGCAGCCAGAGCCACAAGTTTTACAGCTATTGTCAGCGACGTTCGCCGGTCAGAACAATCAAATCTT
CGCCGTCGATTATGGAGTCTGGGGCTTTCGACTGGTCGATGCACTCTTCATTGTTCTGAACGATATTGAACAGATTCTCT
CTTCACGTTCGCTTAACACGGCAGGGGATTTTCGTTGA

Upstream 100 bases:

>100_bases
CTGTGGCTGTTGCGCGGAAGACCAACATTAGCAGCAGTGCCGGTGCTGGAAGCGGCGGAACTGGTGGATGGAAGTACCAC
GAATTGAAAGGAACACTCGC

Downstream 100 bases:

>100_bases
CAGATAGGTATAAAGCAAGAGGCACATCACAATGGACACGCAACACATTGCCACAGACACATGGAGCATCGATCATCTTG
CCCCCCACGCTCCGGCCACG

Product: periplasmic binding protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MCAATLIDTTDALLSRYDQLDLRGLSITEIAELLRQQGAAHLSPIERIELAERLITRRRFLIGAGALALGIITGCGTQEQ
AAAPTAMVATTRTVVDGLGRRVEVPAVPQRVVALHDLDNAYALASLGFAPIGMGSITLGDPFERLRSLGPIPPALEQTVE
VGLLYEPNLEAIASLQPDLILGTRGSHQQILDQLTAIAPTVLIDQTAGDDVLSNQRFLASLVGVEDRIDEQLARYEARIA
ALRERYADTLNTLEYTRIDSYGVGAEDNYLILFDLTPGTRVLTDLGARRSKTNGNVDNDPFPAISLERLADYDADVIFIG
VEAGTQPEPQVLQLLSATFAGQNNQIFAVDYGVWGFRLVDALFIVLNDIEQILSSRSLNTAGDFR

Sequences:

>Translated_385_residues
MCAATLIDTTDALLSRYDQLDLRGLSITEIAELLRQQGAAHLSPIERIELAERLITRRRFLIGAGALALGIITGCGTQEQ
AAAPTAMVATTRTVVDGLGRRVEVPAVPQRVVALHDLDNAYALASLGFAPIGMGSITLGDPFERLRSLGPIPPALEQTVE
VGLLYEPNLEAIASLQPDLILGTRGSHQQILDQLTAIAPTVLIDQTAGDDVLSNQRFLASLVGVEDRIDEQLARYEARIA
ALRERYADTLNTLEYTRIDSYGVGAEDNYLILFDLTPGTRVLTDLGARRSKTNGNVDNDPFPAISLERLADYDADVIFIG
VEAGTQPEPQVLQLLSATFAGQNNQIFAVDYGVWGFRLVDALFIVLNDIEQILSSRSLNTAGDFR
>Mature_385_residues
MCAATLIDTTDALLSRYDQLDLRGLSITEIAELLRQQGAAHLSPIERIELAERLITRRRFLIGAGALALGIITGCGTQEQ
AAAPTAMVATTRTVVDGLGRRVEVPAVPQRVVALHDLDNAYALASLGFAPIGMGSITLGDPFERLRSLGPIPPALEQTVE
VGLLYEPNLEAIASLQPDLILGTRGSHQQILDQLTAIAPTVLIDQTAGDDVLSNQRFLASLVGVEDRIDEQLARYEARIA
ALRERYADTLNTLEYTRIDSYGVGAEDNYLILFDLTPGTRVLTDLGARRSKTNGNVDNDPFPAISLERLADYDADVIFIG
VEAGTQPEPQVLQLLSATFAGQNNQIFAVDYGVWGFRLVDALFIVLNDIEQILSSRSLNTAGDFR

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=257, Percent_Identity=26.8482490272374, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 41740; Mature: 41740

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAATLIDTTDALLSRYDQLDLRGLSITEIAELLRQQGAAHLSPIERIELAERLITRRRF
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LIGAGALALGIITGCGTQEQAAAPTAMVATTRTVVDGLGRRVEVPAVPQRVVALHDLDNA
HHCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCH
YALASLGFAPIGMGSITLGDPFERLRSLGPIPPALEQTVEVGLLYEPNLEAIASLQPDLI
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHEEEEECCCHHHHHHCCCCEE
LGTRGSHQQILDQLTAIAPTVLIDQTAGDDVLSNQRFLASLVGVEDRIDEQLARYEARIA
EECCCCHHHHHHHHHHHCCHHEEECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRERYADTLNTLEYTRIDSYGVGAEDNYLILFDLTPGTRVLTDLGARRSKTNGNVDNDP
HHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCC
FPAISLERLADYDADVIFIGVEAGTQPEPQVLQLLSATFAGQNNQIFAVDYGVWGFRLVD
CCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
ALFIVLNDIEQILSSRSLNTAGDFR
HHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MCAATLIDTTDALLSRYDQLDLRGLSITEIAELLRQQGAAHLSPIERIELAERLITRRRF
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LIGAGALALGIITGCGTQEQAAAPTAMVATTRTVVDGLGRRVEVPAVPQRVVALHDLDNA
HHCCHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCH
YALASLGFAPIGMGSITLGDPFERLRSLGPIPPALEQTVEVGLLYEPNLEAIASLQPDLI
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHEEEEECCCHHHHHHCCCCEE
LGTRGSHQQILDQLTAIAPTVLIDQTAGDDVLSNQRFLASLVGVEDRIDEQLARYEARIA
EECCCCHHHHHHHHHHHCCHHEEECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRERYADTLNTLEYTRIDSYGVGAEDNYLILFDLTPGTRVLTDLGARRSKTNGNVDNDP
HHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCC
FPAISLERLADYDADVIFIGVEAGTQPEPQVLQLLSATFAGQNNQIFAVDYGVWGFRLVD
CCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
ALFIVLNDIEQILSSRSLNTAGDFR
HHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]