Click here to switch to the map view.

The map label for this gene is glpK [H]

Identifier: 222527335

GI number: 222527335

Start: 5103403

End: 5104887

Strand: Reverse

Name: glpK [H]

Synonym: Chy400_4121

Alternate gene names: 222527335

Gene position: 5104887-5103403 (Counterclockwise)

Preceding gene: 222527337

Following gene: 222527334

Centisome position: 96.89

GC content: 61.55

Gene sequence:

>1485_bases
GTGAGTAAGCGCTATCTTCTCGCGATTGATCAGGGTGGTAGTGGCAGTCGGGCGGTGATTTACGATCCCGATGGCCAGGT
GCGTGGTTATGGCTATCGGCCTGTGGGGCGCGTGTATCCACAACCGGGGTGGGTCGAGCAGCGTCCGGCAGCGATTGCCC
GCAGTGTTGCTGAAGCAATTCAGGAAGCGTTAGGGCGGGCCGGTGTGCGGGGGAACGAACTGCTGGCCTGCGGGATCACC
TCACAACGCGATACAGTCTTTGCCTGGCACGCAATCAGTGGCCGACCAATTGGGAATGCCATTACCTGGCAAGACCTGCG
CACGGCGCCAATGGTTGCAGCGTTAGAACAGACCCCGTTAGGGCCGCAGCGCCGCGAGCGGTTGGGTCAGTTTCCCGGTG
CGTATGCCGGGGCAATGCATATGGCCTGGCGGATGCAACACGATGCAGCCTTTCGTCGCGCTGCTGAACGTGGGGTGTTG
CGCGTGTCGCTGGCCGCGGGTTGGATCGTGCAGGCGCTTGGTCAGCCCTGCGAGCATGCGCTTGATCATTCGCTGTTGCA
GGCCATGACAGTCTACGATCCGCGCCGACGTGCGCTGTGGGATGAATGGATTGACGCCTTGAATCTGCCGCGCGCAGCGT
TACCGGTTGCCCGTCCAACCATCCATCATTTTGGCGATCTGGCCGTGGATGGAGCGATGGTGCCGGTGCTGGCAATGATT
ACCGATCAACAATCGGCGCTGTTTGGTTATGATTGTCGTGCACCTGGCCAGGCAGTGGCGACGCACGGGACAGCATCATT
TGTGAATGCGGTGATTGGTTCTTCACCACCGCCCCAGGGCATCTGTAAGACCTACCTGGCCTGGGAGATTGCAGGTGTGG
CAACCTATGCCCTCGAAGCCGATATGACCACGACCGGCGCGGCTGCGAACTGGCTACGTGAGCTTGGTCTGGTGCGGCGG
GTGACCGATCTCGACCGTCACGCTGCCCGGGTTTCGGATAGTGGTGGGGTCGTGTTTGTGCCGGCACTGAATGGGTTAGG
CGTACCCAGTGAAGACCGGAGTGCTCGTGGTGCTATCTTCGGGCTGGCGTTGGGGGTGGATGTCGGTCATATCGCCAGGG
CATTCTACGAAGCCATCGGCTTTCAATTGGTTGACATTCTGGCGACGATGCGGGCCGAAGCCGGGATTAGCGTAGAAGAG
TTGCGCGTAGGGGGGGGGCTGGCTGCCAGTGATCTGGCCTGTCAGATTCAAGCCGATCTGAGCGGGGTTCCCCTGGTGCG
AGCGGCTGATACCGAGACGACGGCACGCGGCGTGGCGTTGCTGGCCGGGATTGGGGCTGGGGTCTGGGCGCAAGAATCGC
TACCACCCTTGATTGACGAGCATGTCCGGCGTTTTGTGCCGCGCCTTTCACCTGCTGAGCGATCTGCGCACTACGAGCGG
TGGCAGAAGGCCCTGGAGCGGGTGCGCGGATGGGTAAAGGTATAG

Upstream 100 bases:

>100_bases
TGATGTGGTAAGTGGATAGGGCAAGGTGCGCTTAACCGTGAGCTTAGTGTATCTTGCTATAATACTCCCCATGTGTTCTT
GCGATAGATGAGGCCAGTCT

Downstream 100 bases:

>100_bases
GCGACGTGCAGCGCCGAAAACTATCTTGCATCGCATGTCAATTTCGTGCCTGATAGCGGTTGCTGAAGCTACCAACACCT
TCTATAATAGAGAAACGATT

Product: carbohydrate kinase FGGY

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSKRYLLAIDQGGSGSRAVIYDPDGQVRGYGYRPVGRVYPQPGWVEQRPAAIARSVAEAIQEALGRAGVRGNELLACGIT
SQRDTVFAWHAISGRPIGNAITWQDLRTAPMVAALEQTPLGPQRRERLGQFPGAYAGAMHMAWRMQHDAAFRRAAERGVL
RVSLAAGWIVQALGQPCEHALDHSLLQAMTVYDPRRRALWDEWIDALNLPRAALPVARPTIHHFGDLAVDGAMVPVLAMI
TDQQSALFGYDCRAPGQAVATHGTASFVNAVIGSSPPPQGICKTYLAWEIAGVATYALEADMTTTGAAANWLRELGLVRR
VTDLDRHAARVSDSGGVVFVPALNGLGVPSEDRSARGAIFGLALGVDVGHIARAFYEAIGFQLVDILATMRAEAGISVEE
LRVGGGLAASDLACQIQADLSGVPLVRAADTETTARGVALLAGIGAGVWAQESLPPLIDEHVRRFVPRLSPAERSAHYER
WQKALERVRGWVKV

Sequences:

>Translated_494_residues
MSKRYLLAIDQGGSGSRAVIYDPDGQVRGYGYRPVGRVYPQPGWVEQRPAAIARSVAEAIQEALGRAGVRGNELLACGIT
SQRDTVFAWHAISGRPIGNAITWQDLRTAPMVAALEQTPLGPQRRERLGQFPGAYAGAMHMAWRMQHDAAFRRAAERGVL
RVSLAAGWIVQALGQPCEHALDHSLLQAMTVYDPRRRALWDEWIDALNLPRAALPVARPTIHHFGDLAVDGAMVPVLAMI
TDQQSALFGYDCRAPGQAVATHGTASFVNAVIGSSPPPQGICKTYLAWEIAGVATYALEADMTTTGAAANWLRELGLVRR
VTDLDRHAARVSDSGGVVFVPALNGLGVPSEDRSARGAIFGLALGVDVGHIARAFYEAIGFQLVDILATMRAEAGISVEE
LRVGGGLAASDLACQIQADLSGVPLVRAADTETTARGVALLAGIGAGVWAQESLPPLIDEHVRRFVPRLSPAERSAHYER
WQKALERVRGWVKV
>Mature_493_residues
SKRYLLAIDQGGSGSRAVIYDPDGQVRGYGYRPVGRVYPQPGWVEQRPAAIARSVAEAIQEALGRAGVRGNELLACGITS
QRDTVFAWHAISGRPIGNAITWQDLRTAPMVAALEQTPLGPQRRERLGQFPGAYAGAMHMAWRMQHDAAFRRAAERGVLR
VSLAAGWIVQALGQPCEHALDHSLLQAMTVYDPRRRALWDEWIDALNLPRAALPVARPTIHHFGDLAVDGAMVPVLAMIT
DQQSALFGYDCRAPGQAVATHGTASFVNAVIGSSPPPQGICKTYLAWEIAGVATYALEADMTTTGAAANWLRELGLVRRV
TDLDRHAARVSDSGGVVFVPALNGLGVPSEDRSARGAIFGLALGVDVGHIARAFYEAIGFQLVDILATMRAEAGISVEEL
RVGGGLAASDLACQIQADLSGVPLVRAADTETTARGVALLAGIGAGVWAQESLPPLIDEHVRRFVPRLSPAERSAHYERW
QKALERVRGWVKV

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=29.2156862745098, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI4504007, Length=507, Percent_Identity=30.3747534516765, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI189409120, Length=507, Percent_Identity=30.3747534516765, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI42794763, Length=512, Percent_Identity=29.8828125, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI41393575, Length=507, Percent_Identity=29.9802761341223, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1790361, Length=498, Percent_Identity=34.3373493975904, Blast_Score=291, Evalue=9e-80,
Organism=Escherichia coli, GI1790005, Length=506, Percent_Identity=25.098814229249, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789987, Length=495, Percent_Identity=26.4646464646465, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17535599, Length=506, Percent_Identity=29.4466403162055, Blast_Score=191, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71986140, Length=530, Percent_Identity=25.4716981132075, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71986147, Length=267, Percent_Identity=31.4606741573034, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321755, Length=555, Percent_Identity=25.5855855855856, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17864214, Length=510, Percent_Identity=30.3921568627451, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24655555, Length=502, Percent_Identity=29.0836653386454, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24655560, Length=502, Percent_Identity=29.0836653386454, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24655570, Length=502, Percent_Identity=29.0836653386454, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24655574, Length=502, Percent_Identity=29.0836653386454, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24655565, Length=502, Percent_Identity=29.0836653386454, Blast_Score=199, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24654573, Length=459, Percent_Identity=30.9368191721133, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI21357867, Length=530, Percent_Identity=27.7358490566038, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24656367, Length=526, Percent_Identity=28.1368821292776, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI19922058, Length=528, Percent_Identity=24.8106060606061, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24656372, Length=375, Percent_Identity=28, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24652871, Length=494, Percent_Identity=24.8987854251012, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI45552909, Length=285, Percent_Identity=27.0175438596491, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 53067; Mature: 52936

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRYLLAIDQGGSGSRAVIYDPDGQVRGYGYRPVGRVYPQPGWVEQRPAAIARSVAEAI
CCCCEEEEEECCCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QEALGRAGVRGNELLACGITSQRDTVFAWHAISGRPIGNAITWQDLRTAPMVAALEQTPL
HHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHCCCC
GPQRRERLGQFPGAYAGAMHMAWRMQHDAAFRRAAERGVLRVSLAAGWIVQALGQPCEHA
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCHHHHH
LDHSLLQAMTVYDPRRRALWDEWIDALNLPRAALPVARPTIHHFGDLAVDGAMVPVLAMI
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHCCHHCCCHHHHHHHHH
TDQQSALFGYDCRAPGQAVATHGTASFVNAVIGSSPPPQGICKTYLAWEIAGVATYALEA
CCCCHHEECCCCCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEEC
DMTTTGAAANWLRELGLVRRVTDLDRHAARVSDSGGVVFVPALNGLGVPSEDRSARGAIF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEE
GLALGVDVGHIARAFYEAIGFQLVDILATMRAEAGISVEELRVGGGLAASDLACQIQADL
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCCCCEEEEECCC
SGVPLVRAADTETTARGVALLAGIGAGVWAQESLPPLIDEHVRRFVPRLSPAERSAHYER
CCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
WQKALERVRGWVKV
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SKRYLLAIDQGGSGSRAVIYDPDGQVRGYGYRPVGRVYPQPGWVEQRPAAIARSVAEAI
CCCEEEEEECCCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
QEALGRAGVRGNELLACGITSQRDTVFAWHAISGRPIGNAITWQDLRTAPMVAALEQTPL
HHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHCCCC
GPQRRERLGQFPGAYAGAMHMAWRMQHDAAFRRAAERGVLRVSLAAGWIVQALGQPCEHA
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCHHHHH
LDHSLLQAMTVYDPRRRALWDEWIDALNLPRAALPVARPTIHHFGDLAVDGAMVPVLAMI
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHCCHHCCCHHHHHHHHH
TDQQSALFGYDCRAPGQAVATHGTASFVNAVIGSSPPPQGICKTYLAWEIAGVATYALEA
CCCCHHEECCCCCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEEEEC
DMTTTGAAANWLRELGLVRRVTDLDRHAARVSDSGGVVFVPALNGLGVPSEDRSARGAIF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEE
GLALGVDVGHIARAFYEAIGFQLVDILATMRAEAGISVEELRVGGGLAASDLACQIQADL
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCCCCEEEEECCC
SGVPLVRAADTETTARGVALLAGIGAGVWAQESLPPLIDEHVRRFVPRLSPAERSAHYER
CCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
WQKALERVRGWVKV
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA