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The map label for this gene is gltA [H]

Identifier: 222527326

GI number: 222527326

Start: 5091357

End: 5092643

Strand: Reverse

Name: gltA [H]

Synonym: Chy400_4112

Alternate gene names: 222527326

Gene position: 5092643-5091357 (Counterclockwise)

Preceding gene: 222527327

Following gene: 222527322

Centisome position: 96.65

GC content: 53.77

Gene sequence:

>1287_bases
ATGACCAGGAACACTCTCACCGTAACCGACAACCGCACCGGTAAAACGTACGAGATTCCCATCGAGAACAACACCATTCG
GGCAACCGATTTACGCCAGATCAAAGTATCGGAAGACGATTTTGGTTTGATGTCCTATGATCCGGCTTACCTCAATACTG
CATCCTGTAAGAGTAGCATCACCTACATCGACGGTGATAAGGGGATTCTAGAGTATCGAGGTTATCCAATTGAGCAACTG
GCTGAGCAGAGTTCGTATCTTGAGGTTGCGTACCTGCTGCTGTACGGGGAACTCCCCTCAAAGGAACGCCTGGCCTGGTG
GGAATACCGGATCAGTCGGCATCTTTTCCTCCACAACAGCCTGGTAGAGCTGATTCAAGCCTTCCGCTACGACGCCCATC
CAATGGGTATTCTCATCAGCTCCGTAGCGGCAATGTCCACGCTATACCCCGAAGCCAAGAACATCCACGATCCGGCTGTG
CGCGAAAAGCAAATCTGGCGTATTATCGGCCAGATCCCGACAATTGCTGCCTTTGCCTACCGCCACCGCATCGGTCGTCC
ATTCAATTTGCCCGATAGCTCGCTCAGCTACACCGCCAACCTGCTGTACATGATGGACTATATGAATCAGCGCGAGTATG
AAGTAAATCCAGTGCTGGCCAAAGCGCTGGATGTGCTCTTCATTTTGCATGCTGATCACGAGCAGAACTGCTCTACCTCG
GTCATGCGTAGTGTTGGCTCAAGCCACGCTGATCCGTACAATGCGCTGGCCGCTGCCGCAGCCGCTCTCTACGGCCCACT
CCACGGTGGTGCCAACGAGGCGGTACTGCGCATGTTGCAGCAGATCGGCCATCCAAAGAACGTGCCGGCATTTATCGAGC
GGGTGAAGAAGGGTGAAATGCGTCTCATGGGCTTTGGTCATCGCGTTTACAAGAATTACGATCCGCGGGCCAAGATCATT
CGTAAGATCGCTCACGAAGTCTTTGCGGCTACTGCTGCCAACCCGCTGCTCGATGTCGCGATGGAGCTTGAGCGAACTGC
ATTGGAAGACGAGTACTTTATCTCGCGCAAGCTCTATCCGAATGTGGATTTCTACAGCGGTCTGATCTATCAGGCACTGC
GCTTCCCAATCGAGTACTTCCCCTTCCTGTTCGCAATTCCACGCGCTTCAGGCTGGCTGGCACAGTGGATCGAGATGCTC
GAAGACCCCGAGCAGAAGATTACCCGTCCACGACAGGTCTATGTTGGCCCACAGCGGCGGGATTATGTGCCGATTGATCA
GCGCTAA

Upstream 100 bases:

>100_bases
TAGAACTACTGTAGTACGGCCTCCATGATGTCGATCACAGTGCGGTAGCACTGATGTATCAATGGCGATGCAGTCGCAGT
GAGCATGAAAGAGCATGGCT

Downstream 100 bases:

>100_bases
TTGAACGATAATAATTATCAGGGCGGTCGTCAGGCCGCCCTGATGATTCGGTAGTCGGTTGGATGAGGGTGAAGACGCAG
GTGGTTGTCGTGGGTGGCGA

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTRNTLTVTDNRTGKTYEIPIENNTIRATDLRQIKVSEDDFGLMSYDPAYLNTASCKSSITYIDGDKGILEYRGYPIEQL
AEQSSYLEVAYLLLYGELPSKERLAWWEYRISRHLFLHNSLVELIQAFRYDAHPMGILISSVAAMSTLYPEAKNIHDPAV
REKQIWRIIGQIPTIAAFAYRHRIGRPFNLPDSSLSYTANLLYMMDYMNQREYEVNPVLAKALDVLFILHADHEQNCSTS
VMRSVGSSHADPYNALAAAAAALYGPLHGGANEAVLRMLQQIGHPKNVPAFIERVKKGEMRLMGFGHRVYKNYDPRAKII
RKIAHEVFAATAANPLLDVAMELERTALEDEYFISRKLYPNVDFYSGLIYQALRFPIEYFPFLFAIPRASGWLAQWIEML
EDPEQKITRPRQVYVGPQRRDYVPIDQR

Sequences:

>Translated_428_residues
MTRNTLTVTDNRTGKTYEIPIENNTIRATDLRQIKVSEDDFGLMSYDPAYLNTASCKSSITYIDGDKGILEYRGYPIEQL
AEQSSYLEVAYLLLYGELPSKERLAWWEYRISRHLFLHNSLVELIQAFRYDAHPMGILISSVAAMSTLYPEAKNIHDPAV
REKQIWRIIGQIPTIAAFAYRHRIGRPFNLPDSSLSYTANLLYMMDYMNQREYEVNPVLAKALDVLFILHADHEQNCSTS
VMRSVGSSHADPYNALAAAAAALYGPLHGGANEAVLRMLQQIGHPKNVPAFIERVKKGEMRLMGFGHRVYKNYDPRAKII
RKIAHEVFAATAANPLLDVAMELERTALEDEYFISRKLYPNVDFYSGLIYQALRFPIEYFPFLFAIPRASGWLAQWIEML
EDPEQKITRPRQVYVGPQRRDYVPIDQR
>Mature_427_residues
TRNTLTVTDNRTGKTYEIPIENNTIRATDLRQIKVSEDDFGLMSYDPAYLNTASCKSSITYIDGDKGILEYRGYPIEQLA
EQSSYLEVAYLLLYGELPSKERLAWWEYRISRHLFLHNSLVELIQAFRYDAHPMGILISSVAAMSTLYPEAKNIHDPAVR
EKQIWRIIGQIPTIAAFAYRHRIGRPFNLPDSSLSYTANLLYMMDYMNQREYEVNPVLAKALDVLFILHADHEQNCSTSV
MRSVGSSHADPYNALAAAAAALYGPLHGGANEAVLRMLQQIGHPKNVPAFIERVKKGEMRLMGFGHRVYKNYDPRAKIIR
KIAHEVFAATAANPLLDVAMELERTALEDEYFISRKLYPNVDFYSGLIYQALRFPIEYFPFLFAIPRASGWLAQWIEMLE
DPEQKITRPRQVYVGPQRRDYVPIDQR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=385, Percent_Identity=26.4935064935065, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1786939, Length=384, Percent_Identity=48.9583333333333, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1786527, Length=380, Percent_Identity=31.0526315789474, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=387, Percent_Identity=26.3565891472868, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324328, Length=326, Percent_Identity=26.6871165644172, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319850, Length=326, Percent_Identity=26.9938650306748, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=396, Percent_Identity=22.979797979798, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24640124, Length=404, Percent_Identity=26.980198019802, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640126, Length=404, Percent_Identity=26.980198019802, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356863, Length=395, Percent_Identity=26.0759493670886, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 49131; Mature: 49000

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNTLTVTDNRTGKTYEIPIENNTIRATDLRQIKVSEDDFGLMSYDPAYLNTASCKSSI
CCCCEEEEECCCCCCEEEEECCCCEEEECCCEEEEECCCCCCCEECCCCCCCCHHHCCCC
TYIDGDKGILEYRGYPIEQLAEQSSYLEVAYLLLYGELPSKERLAWWEYRISRHLFLHNS
EEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LVELIQAFRYDAHPMGILISSVAAMSTLYPEAKNIHDPAVREKQIWRIIGQIPTIAAFAY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
RHRIGRPFNLPDSSLSYTANLLYMMDYMNQREYEVNPVLAKALDVLFILHADHEQNCSTS
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCCCCHHHH
VMRSVGSSHADPYNALAAAAAALYGPLHGGANEAVLRMLQQIGHPKNVPAFIERVKKGEM
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCE
RLMGFGHRVYKNYDPRAKIIRKIAHEVFAATAANPLLDVAMELERTALEDEYFISRKLYP
EEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCC
NVDFYSGLIYQALRFPIEYFPFLFAIPRASGWLAQWIEMLEDPEQKITRPRQVYVGPQRR
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCCCEEEECCCCC
DYVPIDQR
CCCCCCCC
>Mature Secondary Structure 
TRNTLTVTDNRTGKTYEIPIENNTIRATDLRQIKVSEDDFGLMSYDPAYLNTASCKSSI
CCCEEEEECCCCCCEEEEECCCCEEEECCCEEEEECCCCCCCEECCCCCCCCHHHCCCC
TYIDGDKGILEYRGYPIEQLAEQSSYLEVAYLLLYGELPSKERLAWWEYRISRHLFLHNS
EEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LVELIQAFRYDAHPMGILISSVAAMSTLYPEAKNIHDPAVREKQIWRIIGQIPTIAAFAY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
RHRIGRPFNLPDSSLSYTANLLYMMDYMNQREYEVNPVLAKALDVLFILHADHEQNCSTS
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCCCCHHHH
VMRSVGSSHADPYNALAAAAAALYGPLHGGANEAVLRMLQQIGHPKNVPAFIERVKKGEM
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCE
RLMGFGHRVYKNYDPRAKIIRKIAHEVFAATAANPLLDVAMELERTALEDEYFISRKLYP
EEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCC
NVDFYSGLIYQALRFPIEYFPFLFAIPRASGWLAQWIEMLEDPEQKITRPRQVYVGPQRR
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCCCEEEECCCCC
DYVPIDQR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]