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The map label for this gene is sucB [C]

Identifier: 222527175

GI number: 222527175

Start: 4905462

End: 4906859

Strand: Reverse

Name: sucB [C]

Synonym: Chy400_3957

Alternate gene names: 222527175

Gene position: 4906859-4905462 (Counterclockwise)

Preceding gene: 222527176

Following gene: 222527174

Centisome position: 93.13

GC content: 60.3

Gene sequence:

>1398_bases
ATGCCTACCGAGGTTGTGATGCCAAAGTGGGGCTTGAGCATGCAAGAGGGAAAGATCAACCTCTGGCTCAAGCGCGAAGG
CGAGTCGGTGCAGCAGGGTGAGCCAATTGCAGAGGTGGAAACGGAGAAGATCACGAATGTCGTTGAAGCTCCGGTGAGTG
GAACGCTGGCGCGGCTGTGTTATCCGGAAGGGAGCGTTGTGGCGGTGACGAAGGTTATCGCCTACATCACTGCTCCCGGT
GAGCGGCTGGTCGAGGTGGCCGGGAATGGCGCAGTAGAGACGGTTCCGGCTCCGGTTGCCGTTCAAGATACGCCGGCAGT
GTCTCTGCCGCCGGCACCAGCCCGTACCGCTGCACCGCGCGCCATGCCGGCGGCGCGCAAGCTGGCCCAAGAGCATGGGA
TTGATTTGAGCACGCTGGTCGGGAGTGGCCCTGGTGGGGCGATTATCCGGGAAGATGTTGAGCGAGCCATTGCAGCCCGG
GCGACGCCAGTACAGCCGCTCCAGCGGGTCGGCTTCTACAGTGATGGTCATCGTCTTGATGGCTTGCTGTACACGCCTCG
TGATCTAGCACCGGGTGAACGCAGGCCAGGTGTGGTATTGCTGGTCGGCTATACCTACCTCAAGACGATGGTGATGCCCG
ACATCGCCAAAGTGCTCAATGCTGCCGGCTATGTTGCACTCGTCTTCGATTACCGTGGGTTTGGCGAGAGTGAAGGAGAG
CGGGGTCGCCTCATGCCACTTGAGCAGGTGGCGGATGCGCGGGCTGCCCTGACATTCCTATCCAACCAGCCGACTGTCGA
TCCTGAGCGCATGGCTGTGATCGGGATCAGTCTGGGTGGTGCGCACGCAGTGACAACCGCTGCCCTTGATCGGCGAGTTC
GTGCAGCGGTGGCATTGGAGCCGCCCGGCAACGGTGCGCGTTGGTTACGGAGTTTGCGTCGGCAGTGGGAATGGAGTCAA
TTCCTGGCGCGCCTGGCTGAAGATCGCCGGCAGCGGGTGCTGACTGGGAAGTCAAGCCTGGTCGATCCGTTAGAGATTGT
ATTACCCGATCCGGAATCGCAGGCGTTTCTCGATCAGGTTGCGGCTGAGTTCCCCCAGATGAAGGTGACGTTATCGCTGG
CAAGTGCTGAAGCGTTGATCGAGTACGCTCCGGAGGATATAGCGGGTAACATTGCCCCGCGACCGTTGCTGGTGATTCAC
GGCGATGCCGATCAGCTTGTGCCGCTGGCAGAAGCGCAGGCTATCGTCGAGCGGGCGGGGGCAACGGCGCGGTTGGAGGT
TATTCCCGGTATGAGCCATTTCAACTGGGTGCTACCGGGAAGTGCAGGATTTATTCGGGTAACGAACAGTATCGTTGAGT
TTCTGCAACAGGCACTCCCTGTTGTCAGGTCATCGTAA

Upstream 100 bases:

>100_bases
CGACACTGGAAGATGCCTATGTGCCTTCGCCTGAGCGGATTGTGGCAGCGGCGCGGGCGACATTTGGCTGATTCTGGGGG
CGCTTGTTGCAGGAGAGATC

Downstream 100 bases:

>100_bases
TCTTCGCCAGCAGGAAGACCGGCAGGATCGGCAACAAAACGCTACCGTGTCTTACCTGCTGCTATGCAGTGGAGCGCGGG
GCAATAGTGCCCCGCGACGT

Product: biotin/lipoyl attachment domain-containing protein

Products: CoA +S-succinyldihydrolipoamide [C]

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MPTEVVMPKWGLSMQEGKINLWLKREGESVQQGEPIAEVETEKITNVVEAPVSGTLARLCYPEGSVVAVTKVIAYITAPG
ERLVEVAGNGAVETVPAPVAVQDTPAVSLPPAPARTAAPRAMPAARKLAQEHGIDLSTLVGSGPGGAIIREDVERAIAAR
ATPVQPLQRVGFYSDGHRLDGLLYTPRDLAPGERRPGVVLLVGYTYLKTMVMPDIAKVLNAAGYVALVFDYRGFGESEGE
RGRLMPLEQVADARAALTFLSNQPTVDPERMAVIGISLGGAHAVTTAALDRRVRAAVALEPPGNGARWLRSLRRQWEWSQ
FLARLAEDRRQRVLTGKSSLVDPLEIVLPDPESQAFLDQVAAEFPQMKVTLSLASAEALIEYAPEDIAGNIAPRPLLVIH
GDADQLVPLAEAQAIVERAGATARLEVIPGMSHFNWVLPGSAGFIRVTNSIVEFLQQALPVVRSS

Sequences:

>Translated_465_residues
MPTEVVMPKWGLSMQEGKINLWLKREGESVQQGEPIAEVETEKITNVVEAPVSGTLARLCYPEGSVVAVTKVIAYITAPG
ERLVEVAGNGAVETVPAPVAVQDTPAVSLPPAPARTAAPRAMPAARKLAQEHGIDLSTLVGSGPGGAIIREDVERAIAAR
ATPVQPLQRVGFYSDGHRLDGLLYTPRDLAPGERRPGVVLLVGYTYLKTMVMPDIAKVLNAAGYVALVFDYRGFGESEGE
RGRLMPLEQVADARAALTFLSNQPTVDPERMAVIGISLGGAHAVTTAALDRRVRAAVALEPPGNGARWLRSLRRQWEWSQ
FLARLAEDRRQRVLTGKSSLVDPLEIVLPDPESQAFLDQVAAEFPQMKVTLSLASAEALIEYAPEDIAGNIAPRPLLVIH
GDADQLVPLAEAQAIVERAGATARLEVIPGMSHFNWVLPGSAGFIRVTNSIVEFLQQALPVVRSS
>Mature_464_residues
PTEVVMPKWGLSMQEGKINLWLKREGESVQQGEPIAEVETEKITNVVEAPVSGTLARLCYPEGSVVAVTKVIAYITAPGE
RLVEVAGNGAVETVPAPVAVQDTPAVSLPPAPARTAAPRAMPAARKLAQEHGIDLSTLVGSGPGGAIIREDVERAIAARA
TPVQPLQRVGFYSDGHRLDGLLYTPRDLAPGERRPGVVLLVGYTYLKTMVMPDIAKVLNAAGYVALVFDYRGFGESEGER
GRLMPLEQVADARAALTFLSNQPTVDPERMAVIGISLGGAHAVTTAALDRRVRAAVALEPPGNGARWLRSLRRQWEWSQF
LARLAEDRRQRVLTGKSSLVDPLEIVLPDPESQAFLDQVAAEFPQMKVTLSLASAEALIEYAPEDIAGNIAPRPLLVIHG
DADQLVPLAEAQAIVERAGATARLEVIPGMSHFNWVLPGSAGFIRVTNSIVEFLQQALPVVRSS

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG1073

COG function: function code R; Hydrolases of the alpha/beta superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli ycjY [H]

Homologues:

Organism=Homo sapiens, GI203098753, Length=173, Percent_Identity=30.635838150289, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI203098816, Length=173, Percent_Identity=30.635838150289, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1786946, Length=156, Percent_Identity=30.1282051282051, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324258, Length=171, Percent_Identity=29.8245614035088, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 2.3.1.61 [C]

Molecular weight: Translated: 49868; Mature: 49737

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEVVMPKWGLSMQEGKINLWLKREGESVQQGEPIAEVETEKITNVVEAPVSGTLARLC
CCCCEECCCCCCCEECCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHC
YPEGSVVAVTKVIAYITAPGERLVEVAGNGAVETVPAPVAVQDTPAVSLPPAPARTAAPR
CCCCCEEHHHHHHHHHCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCC
AMPAARKLAQEHGIDLSTLVGSGPGGAIIREDVERAIAARATPVQPLQRVGFYSDGHRLD
CCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCC
GLLYTPRDLAPGERRPGVVLLVGYTYLKTMVMPDIAKVLNAAGYVALVFDYRGFGESEGE
CEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
RGRLMPLEQVADARAALTFLSNQPTVDPERMAVIGISLGGAHAVTTAALDRRVRAAVALE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEEEEEEC
PPGNGARWLRSLRRQWEWSQFLARLAEDRRQRVLTGKSSLVDPLEIVLPDPESQAFLDQV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHH
AAEFPQMKVTLSLASAEALIEYAPEDIAGNIAPRPLLVIHGDADQLVPLAEAQAIVERAG
HHHCCCEEEEEEHHHHHHHHHHCCHHHCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCC
ATARLEVIPGMSHFNWVLPGSAGFIRVTNSIVEFLQQALPVVRSS
CCEEEEEECCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTEVVMPKWGLSMQEGKINLWLKREGESVQQGEPIAEVETEKITNVVEAPVSGTLARLC
CCCEECCCCCCCEECCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHC
YPEGSVVAVTKVIAYITAPGERLVEVAGNGAVETVPAPVAVQDTPAVSLPPAPARTAAPR
CCCCCEEHHHHHHHHHCCCCHHHHHHCCCCCEEECCCCEEECCCCCCCCCCCCCCCCCCC
AMPAARKLAQEHGIDLSTLVGSGPGGAIIREDVERAIAARATPVQPLQRVGFYSDGHRLD
CCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCC
GLLYTPRDLAPGERRPGVVLLVGYTYLKTMVMPDIAKVLNAAGYVALVFDYRGFGESEGE
CEEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
RGRLMPLEQVADARAALTFLSNQPTVDPERMAVIGISLGGAHAVTTAALDRRVRAAVALE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEEEEEEC
PPGNGARWLRSLRRQWEWSQFLARLAEDRRQRVLTGKSSLVDPLEIVLPDPESQAFLDQV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHH
AAEFPQMKVTLSLASAEALIEYAPEDIAGNIAPRPLLVIHGDADQLVPLAEAQAIVERAG
HHHCCCEEEEEEHHHHHHHHHHCCHHHCCCCCCCCEEEEECCHHHCCCHHHHHHHHHHCC
ATARLEVIPGMSHFNWVLPGSAGFIRVTNSIVEFLQQALPVVRSS
CCEEEEEECCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl-protein polypeptide chain, covalently bound lipoyl moiety [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; dihydrolipoamide [C]

Specific reaction: Succinyl-CoA + dihydrolipoamide = CoA +S-succinyldihydrolipoamide [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1916281 [H]