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The map label for this gene is ubiE [H]

Identifier: 222527149

GI number: 222527149

Start: 4879513

End: 4880235

Strand: Reverse

Name: ubiE [H]

Synonym: Chy400_3931

Alternate gene names: 222527149

Gene position: 4880235-4879513 (Counterclockwise)

Preceding gene: 222527150

Following gene: 222527148

Centisome position: 92.62

GC content: 58.92

Gene sequence:

>723_bases
ATGAATGTACTACCACCGCCGGAAGCAAAAGCGGCGTATGTTGAGCAAATGTTTGCCCGCATTGCCCGCGGCTACGACCG
GGTCAATCGGGTGATGACGTTTGGGCTTGATCAGGGCTGGCGGCGTAAAGTGGTGGAATATGTTGCGCCACCGGTACACG
GTCGTGCGCTTGATGTTGGTACCGGTACCGGTGATTTTTTGATCGAACTGGCAGCCTGGGCCCGTGAAGGGCTGGTGGTC
GGGGTTGATTTTACCGTGCCCATGATGCAGGCCGGCCAACCGAAAATTGCTGACCTGGCCCAAAGTGGACGTGCTGCCTT
TGTGGCCGGTGATGCGTTGCAGTTGCCGTTTCCCGACAACACCTTCGATGCGATTACCACCGGTTTCGTTTTGCGGAATG
TCACCGACATCCCGGCAGCGCTGCGGGAGATGTACCGGGTGGCCCGGCCCGGTGCCACCATGGCGTGTCTGGAAGTAGCT
CGACCACGGCATCCATTGCTGCGCTGGGGGCACCGTATCTATTTTGAGCACGTTGTACCGTGGATCGGGGCCTTGCTGGG
TGGTGATCGCCGCGCCTACACCTATCTCCCACAATCGGCGCGTGCCTTTCCGCCTCCCGATGATCTGGCGGCAATGATGC
GTGAAGCGGGCTGGAGTCACGTCTTTTACCGCCTCTATGGCCTGGGTGCGGTTGCAATTCATGTCGGTGCAAAAAAGATG
TAG

Upstream 100 bases:

>100_bases
CTTTCGTTAGTGTGTTGATTTGGTTTGATAGGGAACATGAGCGTATTAAATCGCGTGAATATATTTCGTTGCATGCGCGA
TGGTAGAGAGGTGAACTGCG

Downstream 100 bases:

>100_bases
TGACAATGACCATCACTTCAAACACTTGCATGAGGTTGCCCCATGTACCACGACGATGATGATGACGATGAGATTCGACA
GCGGGCCAGACGGCTGATGC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MNVLPPPEAKAAYVEQMFARIARGYDRVNRVMTFGLDQGWRRKVVEYVAPPVHGRALDVGTGTGDFLIELAAWAREGLVV
GVDFTVPMMQAGQPKIADLAQSGRAAFVAGDALQLPFPDNTFDAITTGFVLRNVTDIPAALREMYRVARPGATMACLEVA
RPRHPLLRWGHRIYFEHVVPWIGALLGGDRRAYTYLPQSARAFPPPDDLAAMMREAGWSHVFYRLYGLGAVAIHVGAKKM

Sequences:

>Translated_240_residues
MNVLPPPEAKAAYVEQMFARIARGYDRVNRVMTFGLDQGWRRKVVEYVAPPVHGRALDVGTGTGDFLIELAAWAREGLVV
GVDFTVPMMQAGQPKIADLAQSGRAAFVAGDALQLPFPDNTFDAITTGFVLRNVTDIPAALREMYRVARPGATMACLEVA
RPRHPLLRWGHRIYFEHVVPWIGALLGGDRRAYTYLPQSARAFPPPDDLAAMMREAGWSHVFYRLYGLGAVAIHVGAKKM
>Mature_240_residues
MNVLPPPEAKAAYVEQMFARIARGYDRVNRVMTFGLDQGWRRKVVEYVAPPVHGRALDVGTGTGDFLIELAAWAREGLVV
GVDFTVPMMQAGQPKIADLAQSGRAAFVAGDALQLPFPDNTFDAITTGFVLRNVTDIPAALREMYRVARPGATMACLEVA
RPRHPLLRWGHRIYFEHVVPWIGALLGGDRRAYTYLPQSARAFPPPDDLAAMMREAGWSHVFYRLYGLGAVAIHVGAKKM

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=269, Percent_Identity=32.7137546468402, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI48994983, Length=233, Percent_Identity=39.0557939914163, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17557039, Length=232, Percent_Identity=34.051724137931, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6323526, Length=248, Percent_Identity=27.0161290322581, Blast_Score=102, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24641702, Length=243, Percent_Identity=32.0987654320988, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26504; Mature: 26504

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLPPPEAKAAYVEQMFARIARGYDRVNRVMTFGLDQGWRRKVVEYVAPPVHGRALDVG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEECC
TGTGDFLIELAAWAREGLVVGVDFTVPMMQAGQPKIADLAQSGRAAFVAGDALQLPFPDN
CCCHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCHHHHHHHCCCEEEEECCEEECCCCCC
TFDAITTGFVLRNVTDIPAALREMYRVARPGATMACLEVARPRHPLLRWGHRIYFEHVVP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
WIGALLGGDRRAYTYLPQSARAFPPPDDLAAMMREAGWSHVFYRLYGLGAVAIHVGAKKM
HHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MNVLPPPEAKAAYVEQMFARIARGYDRVNRVMTFGLDQGWRRKVVEYVAPPVHGRALDVG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEECC
TGTGDFLIELAAWAREGLVVGVDFTVPMMQAGQPKIADLAQSGRAAFVAGDALQLPFPDN
CCCHHHHHHHHHHHHCCEEEEEECCCHHHHCCCCHHHHHHHCCCEEEEECCEEECCCCCC
TFDAITTGFVLRNVTDIPAALREMYRVARPGATMACLEVARPRHPLLRWGHRIYFEHVVP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHH
WIGALLGGDRRAYTYLPQSARAFPPPDDLAAMMREAGWSHVFYRLYGLGAVAIHVGAKKM
HHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA