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The map label for this gene is puuB [H]

Identifier: 222527112

GI number: 222527112

Start: 4841544

End: 4842668

Strand: Direct

Name: puuB [H]

Synonym: Chy400_3893

Alternate gene names: 222527112

Gene position: 4841544-4842668 (Clockwise)

Preceding gene: 222527106

Following gene: 222527114

Centisome position: 91.89

GC content: 60.0

Gene sequence:

>1125_bases
ATGCACAACGAACCGATTTGGAATGATGGGACAATTCCCACTTTTCCGGCACTCAACGATGAGATTGCGGTTGACGTATG
CGTGATTGGGTTAGGCGGATCGGGGTTAAGCTGTATTTATGAACTGCTGACCCTTGGCGTTCGTGTGGCCGGCATTGATG
CCTCGACTGTCGGTGGTGGGGCGGCAGGGCGGAACGGTGGATTTTTGCTTGCCGGTCTGGCCGCGTTCTATCACAACGCA
GTAGCCAAACTTGGTCGCGAGCGGGTGGCTGCTATTTATCGCTTGACAATCAGTGAAATCGAACGAATGGCAGCACTGAT
GCCGACGATTGTGCGCCAGGTGGGGTCGTTGCGTATCGCCGCATCGCCAGAAGAACTTGCCGATTGCACAGCGCAGATGA
CAGCAATGCGTGCCGATGATCTGCCGGTCGAAGCGTATCGCGGGCCAGAAGGCGAAGGTCTGCTCATACCAACCGATGGC
GCATTCCAGCCGTTACACCGCTGCATCTGGCTGGCCCGGCAGGTCGAGCGCGGCGGTGCGCTCTTGTTTACGCACACGCC
AGCGTTAGACATCAGCGGGACAACGGTTCACTGTTCGCAAGGCACCATCCATGCCCGCCAGGTCATCGTCGCCGTTGACG
GTGGTCTTGAGCGATTACTGCCCGAACTCCGCGCTACCGTGCGCACCACTCGCCTCCAAATGCTGGCGACAGCCCCCGAT
CCGCATTGCACTATTCCCCGGCCCGTGTACACCCGCTGGGGTTACGACTACTGGCAGCAACTTCCCGATGGTCGCATTGC
GCTGGGCGGTTGTCGTGACCGCTTTGCGGAGAGCGAATGGGATGCGCCGCCACAACCCTCACCGGCAGTCCAGTCTGCGC
TCGAACAGATACTGCGGGAACGGATCGGTAGCCAGGCAGCGATTGAACGGCGCTGGGCTGCTCAGGTGGCGTACCGTCGC
GATAGCATCCTGCCGATTGCCCGCCAGGTTCGCCCCGGCGTTTGGGCTATCGGTGCCTACAACGGTACCGGCAACGTGAT
CGGTGCGATCTGTGGTCGAGCTGTAGCGCATGCCGCTATTCGTGGACAAACGCAGTTGCTGGATTTGTTGGGGATGGATG
GCTGA

Upstream 100 bases:

>100_bases
CAGACCGAGTGCGTTGCCGGGCCACCAGGTCATCATCGCGTATAATTTTTCATCAGGTTCAGGGAGAAGTACCCCTGCGC
AGGAAGCAATGGAGCCACTG

Downstream 100 bases:

>100_bases
TCCGCTCACCGGCATGACAGTAGGGGCAGCGCATGCGTCGCCCCTACAACTCTCAAATATGGAAGAATAACTATGCACTG
CTGGACGCCAGCGCTGGCAT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MHNEPIWNDGTIPTFPALNDEIAVDVCVIGLGGSGLSCIYELLTLGVRVAGIDASTVGGGAAGRNGGFLLAGLAAFYHNA
VAKLGRERVAAIYRLTISEIERMAALMPTIVRQVGSLRIAASPEELADCTAQMTAMRADDLPVEAYRGPEGEGLLIPTDG
AFQPLHRCIWLARQVERGGALLFTHTPALDISGTTVHCSQGTIHARQVIVAVDGGLERLLPELRATVRTTRLQMLATAPD
PHCTIPRPVYTRWGYDYWQQLPDGRIALGGCRDRFAESEWDAPPQPSPAVQSALEQILRERIGSQAAIERRWAAQVAYRR
DSILPIARQVRPGVWAIGAYNGTGNVIGAICGRAVAHAAIRGQTQLLDLLGMDG

Sequences:

>Translated_374_residues
MHNEPIWNDGTIPTFPALNDEIAVDVCVIGLGGSGLSCIYELLTLGVRVAGIDASTVGGGAAGRNGGFLLAGLAAFYHNA
VAKLGRERVAAIYRLTISEIERMAALMPTIVRQVGSLRIAASPEELADCTAQMTAMRADDLPVEAYRGPEGEGLLIPTDG
AFQPLHRCIWLARQVERGGALLFTHTPALDISGTTVHCSQGTIHARQVIVAVDGGLERLLPELRATVRTTRLQMLATAPD
PHCTIPRPVYTRWGYDYWQQLPDGRIALGGCRDRFAESEWDAPPQPSPAVQSALEQILRERIGSQAAIERRWAAQVAYRR
DSILPIARQVRPGVWAIGAYNGTGNVIGAICGRAVAHAAIRGQTQLLDLLGMDG
>Mature_374_residues
MHNEPIWNDGTIPTFPALNDEIAVDVCVIGLGGSGLSCIYELLTLGVRVAGIDASTVGGGAAGRNGGFLLAGLAAFYHNA
VAKLGRERVAAIYRLTISEIERMAALMPTIVRQVGSLRIAASPEELADCTAQMTAMRADDLPVEAYRGPEGEGLLIPTDG
AFQPLHRCIWLARQVERGGALLFTHTPALDISGTTVHCSQGTIHARQVIVAVDGGLERLLPELRATVRTTRLQMLATAPD
PHCTIPRPVYTRWGYDYWQQLPDGRIALGGCRDRFAESEWDAPPQPSPAVQSALEQILRERIGSQAAIERRWAAQVAYRR
DSILPIARQVRPGVWAIGAYNGTGNVIGAICGRAVAHAAIRGQTQLLDLLGMDG

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=384, Percent_Identity=23.6979166666667, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40178; Mature: 40178

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNEPIWNDGTIPTFPALNDEIAVDVCVIGLGGSGLSCIYELLTLGVRVAGIDASTVGGG
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCEEEECCCCCCCCCC
AAGRNGGFLLAGLAAFYHNAVAKLGRERVAAIYRLTISEIERMAALMPTIVRQVGSLRIA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
ASPEELADCTAQMTAMRADDLPVEAYRGPEGEGLLIPTDGAFQPLHRCIWLARQVERGGA
CCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCE
LLFTHTPALDISGTTVHCSQGTIHARQVIVAVDGGLERLLPELRATVRTTRLQMLATAPD
EEEEECCCEECCCCEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEECCC
PHCTIPRPVYTRWGYDYWQQLPDGRIALGGCRDRFAESEWDAPPQPSPAVQSALEQILRE
CCCCCCCCHHHHCCHHHHHHCCCCCEEECCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RIGSQAAIERRWAAQVAYRRDSILPIARQVRPGVWAIGAYNGTGNVIGAICGRAVAHAAI
HCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
RGQTQLLDLLGMDG
CCHHHHHHHHCCCC
>Mature Secondary Structure
MHNEPIWNDGTIPTFPALNDEIAVDVCVIGLGGSGLSCIYELLTLGVRVAGIDASTVGGG
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCEEEECCCCCCCCCC
AAGRNGGFLLAGLAAFYHNAVAKLGRERVAAIYRLTISEIERMAALMPTIVRQVGSLRIA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
ASPEELADCTAQMTAMRADDLPVEAYRGPEGEGLLIPTDGAFQPLHRCIWLARQVERGGA
CCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCE
LLFTHTPALDISGTTVHCSQGTIHARQVIVAVDGGLERLLPELRATVRTTRLQMLATAPD
EEEEECCCEECCCCEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEECCC
PHCTIPRPVYTRWGYDYWQQLPDGRIALGGCRDRFAESEWDAPPQPSPAVQSALEQILRE
CCCCCCCCHHHHCCHHHHHHCCCCCEEECCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RIGSQAAIERRWAAQVAYRRDSILPIARQVRPGVWAIGAYNGTGNVIGAICGRAVAHAAI
HCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
RGQTQLLDLLGMDG
CCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]