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The map label for this gene is yfiU [H]

Identifier: 222527009

GI number: 222527009

Start: 4719124

End: 4720506

Strand: Direct

Name: yfiU [H]

Synonym: Chy400_3788

Alternate gene names: 222527009

Gene position: 4719124-4720506 (Clockwise)

Preceding gene: 222527005

Following gene: 222527010

Centisome position: 89.56

GC content: 59.8

Gene sequence:

>1383_bases
ATGAGCGATGAACGTCGGCGGAATCAAATCCTGGGTCTTCTCTTTGTTGGTGTTTTGATGGCCGCGCTCGATATTGCGAT
TGTCGGGCCGGCATTGCCCGCGATCCAGCGCGTCTTTGGGGTTGATGAGCGGGCGTTGTCCTGGGTCTTTTCGATCTATG
TCCTGGGCAACCTGGTTGGCACCCCGACCATTGCCGCACTATCGGATCGGTTTGGTCGGCGAGCGTTGTACGTCGTGAGC
CTGAGTGGCTTTGCACTGGGGTCACTGCTCGTTGCCATTGCCCCCTCTTTTCCCATGCTCCTCTTAGGCCGTCTGGTGCA
GGGGGTAAGTGCCGGTGGCGTGATTCCGGTTGCCAGTGCCGTTATCGGTGATACGTTTCCCCCTGAACGGCGCGGCTCGG
CCCTGGGATTGATCGGGGCTGTGTTTGGGATTGCGTTCTTGATCGGGCCAATTATCGGTGGATTGCTCCTCTTGATCGGC
TGGCAATGGCTCTTCCTGATCAATCTGCCGCTGGCCGCAGTGCTGATCATCTTCAGCCTGCGCCTTTTGCCGGGTCGCAC
GCGCCAGTCTGCGGCAACCTTTGATCTGGCCGGCCTTATTGTGCTCGGACTGATGCTCACCGGTCTGGCGTATGGTTTGA
CCGAACTCGATCCGGCGCGATTGAGCAACGGATCGCTACCGCTGCCGGCAATTGGTGCATTGATCGTGGCGTTCGCGCTG
GCACCGATCTTCATTGCTATCGAGCAACGGGCCGGTGAACCCATCCTGCAACCGGCCATCTTTCGTTCACGCCAAATCTG
GATAACGGCAGTTCTGGCAATCGGCGCGGGGATTGCCGAGTCTGCCGTTGTCTTTGTGCCGGCACTGCTCACGGCGGCCT
ACGGTGTCAGCAGTTCGACGGCCAGTTTCATGTTGCTACCGGTGGTGCTGGCGATGGCGATTGGGTCACCGGTTTCGGGT
CGCCTGCTCGACCGCTTCGGCTCGCGGATTGTGGTGAGTATTGGTGTCGCGCTGAGTGGAATCGGGATGCTGATCCTGAG
CACATGGCCAACGAGTCTGGTGGCATTCTACCTCGCAGCGGTCGTCAGCGGTTTAGGTCTGGCGATCTTGCTTGGGGCCG
CCTTGCGGTATATTCTGCTGAATGAAGTATCGGCAAACGAGCGGGCTGCCGCTCAAGGACTGCTCACCGTTACGATGGGG
AGTGGTCAATTGTTGGGAGCCGTCCTGGTAGGTGTGCTGGCTGCAACCGGAGGTGGCGCTGTTGCCGGCTATGCGCTGGC
CTTCTTCGTGATCGGGATTGTGATGCTCTTGCTCACACCGTTCGCACTGGCCCTCAAGAGCCGTAGTGCCGAGCGGGCAA
CTGCGATGGCGCACGCCCATTGA

Upstream 100 bases:

>100_bases
TAGAGAGGAATGTCAAACCAGAAACGCTGTCACTCGACTGTGTTATAATACTTACCGACCGGTCGGTTGGTATTACAAAG
TACGTCAAGGAGCGCTGATT

Downstream 100 bases:

>100_bases
TCAGGTTTATCCAATCTGGCGTGATCGTGGTCATCGTATGCTATCCCCCGATCACGCCGGCATCGTCTCTGAGTATAGCT
GCGCAGCACCGGCATTTCTG

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSDERRRNQILGLLFVGVLMAALDIAIVGPALPAIQRVFGVDERALSWVFSIYVLGNLVGTPTIAALSDRFGRRALYVVS
LSGFALGSLLVAIAPSFPMLLLGRLVQGVSAGGVIPVASAVIGDTFPPERRGSALGLIGAVFGIAFLIGPIIGGLLLLIG
WQWLFLINLPLAAVLIIFSLRLLPGRTRQSAATFDLAGLIVLGLMLTGLAYGLTELDPARLSNGSLPLPAIGALIVAFAL
APIFIAIEQRAGEPILQPAIFRSRQIWITAVLAIGAGIAESAVVFVPALLTAAYGVSSSTASFMLLPVVLAMAIGSPVSG
RLLDRFGSRIVVSIGVALSGIGMLILSTWPTSLVAFYLAAVVSGLGLAILLGAALRYILLNEVSANERAAAQGLLTVTMG
SGQLLGAVLVGVLAATGGGAVAGYALAFFVIGIVMLLLTPFALALKSRSAERATAMAHAH

Sequences:

>Translated_460_residues
MSDERRRNQILGLLFVGVLMAALDIAIVGPALPAIQRVFGVDERALSWVFSIYVLGNLVGTPTIAALSDRFGRRALYVVS
LSGFALGSLLVAIAPSFPMLLLGRLVQGVSAGGVIPVASAVIGDTFPPERRGSALGLIGAVFGIAFLIGPIIGGLLLLIG
WQWLFLINLPLAAVLIIFSLRLLPGRTRQSAATFDLAGLIVLGLMLTGLAYGLTELDPARLSNGSLPLPAIGALIVAFAL
APIFIAIEQRAGEPILQPAIFRSRQIWITAVLAIGAGIAESAVVFVPALLTAAYGVSSSTASFMLLPVVLAMAIGSPVSG
RLLDRFGSRIVVSIGVALSGIGMLILSTWPTSLVAFYLAAVVSGLGLAILLGAALRYILLNEVSANERAAAQGLLTVTMG
SGQLLGAVLVGVLAATGGGAVAGYALAFFVIGIVMLLLTPFALALKSRSAERATAMAHAH
>Mature_459_residues
SDERRRNQILGLLFVGVLMAALDIAIVGPALPAIQRVFGVDERALSWVFSIYVLGNLVGTPTIAALSDRFGRRALYVVSL
SGFALGSLLVAIAPSFPMLLLGRLVQGVSAGGVIPVASAVIGDTFPPERRGSALGLIGAVFGIAFLIGPIIGGLLLLIGW
QWLFLINLPLAAVLIIFSLRLLPGRTRQSAATFDLAGLIVLGLMLTGLAYGLTELDPARLSNGSLPLPAIGALIVAFALA
PIFIAIEQRAGEPILQPAIFRSRQIWITAVLAIGAGIAESAVVFVPALLTAAYGVSSSTASFMLLPVVLAMAIGSPVSGR
LLDRFGSRIVVSIGVALSGIGMLILSTWPTSLVAFYLAAVVSGLGLAILLGAALRYILLNEVSANERAAAQGLLTVTMGS
GQLLGAVLVGVLAATGGGAVAGYALAFFVIGIVMLLLTPFALALKSRSAERATAMAHAH

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=451, Percent_Identity=25.9423503325942, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788710, Length=320, Percent_Identity=30.625, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789042, Length=323, Percent_Identity=27.2445820433437, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=26.7469879518072, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788392, Length=425, Percent_Identity=25.6470588235294, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081723, Length=174, Percent_Identity=32.183908045977, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790146, Length=146, Percent_Identity=30.8219178082192, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082320, Length=170, Percent_Identity=27.0588235294118, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=326, Percent_Identity=26.6871165644172, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=173, Percent_Identity=31.2138728323699, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=162, Percent_Identity=31.4814814814815, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=31.3333333333333, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=492, Percent_Identity=23.780487804878, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17738085, Length=159, Percent_Identity=28.9308176100629, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47356; Mature: 47225

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDERRRNQILGLLFVGVLMAALDIAIVGPALPAIQRVFGVDERALSWVFSIYVLGNLVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
TPTIAALSDRFGRRALYVVSLSGFALGSLLVAIAPSFPMLLLGRLVQGVSAGGVIPVASA
CCHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHH
VIGDTFPPERRGSALGLIGAVFGIAFLIGPIIGGLLLLIGWQWLFLINLPLAAVLIIFSL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLPGRTRQSAATFDLAGLIVLGLMLTGLAYGLTELDPARLSNGSLPLPAIGALIVAFAL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHH
APIFIAIEQRAGEPILQPAIFRSRQIWITAVLAIGAGIAESAVVFVPALLTAAYGVSSST
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCH
ASFMLLPVVLAMAIGSPVSGRLLDRFGSRIVVSIGVALSGIGMLILSTWPTSLVAFYLAA
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VVSGLGLAILLGAALRYILLNEVSANERAAAQGLLTVTMGSGQLLGAVLVGVLAATGGGA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCH
VAGYALAFFVIGIVMLLLTPFALALKSRSAERATAMAHAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
SDERRRNQILGLLFVGVLMAALDIAIVGPALPAIQRVFGVDERALSWVFSIYVLGNLVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
TPTIAALSDRFGRRALYVVSLSGFALGSLLVAIAPSFPMLLLGRLVQGVSAGGVIPVASA
CCHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHH
VIGDTFPPERRGSALGLIGAVFGIAFLIGPIIGGLLLLIGWQWLFLINLPLAAVLIIFSL
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLPGRTRQSAATFDLAGLIVLGLMLTGLAYGLTELDPARLSNGSLPLPAIGALIVAFAL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHH
APIFIAIEQRAGEPILQPAIFRSRQIWITAVLAIGAGIAESAVVFVPALLTAAYGVSSST
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCH
ASFMLLPVVLAMAIGSPVSGRLLDRFGSRIVVSIGVALSGIGMLILSTWPTSLVAFYLAA
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VVSGLGLAILLGAALRYILLNEVSANERAAAQGLLTVTMGSGQLLGAVLVGVLAATGGGA
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCH
VAGYALAFFVIGIVMLLLTPFALALKSRSAERATAMAHAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]