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The map label for this gene is gacS [H]

Identifier: 222526964

GI number: 222526964

Start: 4664555

End: 4665511

Strand: Direct

Name: gacS [H]

Synonym: Chy400_3742

Alternate gene names: 222526964

Gene position: 4664555-4665511 (Clockwise)

Preceding gene: 222526963

Following gene: 222526965

Centisome position: 88.53

GC content: 49.53

Gene sequence:

>957_bases
ATGTTATTTCTCGTGTATGGTGTGGTATGGGAATATCGAGGGTGGGGTGTAGTACTAACAATCCTTACCATCATTCTGAT
GATCGGGTTGTGGCAATATTTCCAGCATCGGATCACAATCCAGCAACAGCGCATCCGCGAACTTGAGCAAGAATTGGCAG
ATGCTCAGGCTGTAGCTGCTCTCGCAACCCGTACCAAAAGTGCGTTTCTGACAAACATCAGCCACGAGTTACGGACACCG
TTAAACACGATTATTGGTTATAGTGAATTGTTACATGAAGATGTGCTTGATGAGCGACTCGCCGTTGATGAGGTGCGTGA
GCGGCTGGAGCGCATTCAGGCTGCGGCCAGGCAGTTGTTGAGCCTGATCAACGACATCATCGAACTGGCGCGGCTGGAGG
CCGGTGATGTTCCGTTGAATTATGAACCATGGCCAATCGGGTTACTGATTAAAGAGGTTTGCGCCACGATTGAGCCACTG
GTAGAACAGCAACAGAATCGCCTCGTGGTTGATGTACAACCAGACCCTGAGATGGTTGTTCACATCGACCGTAAAAAGGT
TCGTCAGGTGTTGTTGCACCTCCTGGATAATGCCGTAAAGTTCACCCGGCAGGGCGAAATCAAATTCATCGTTACAATTC
GGCGAAGTGATGATGGAACTGAATTATTGATCTGTAACATCAGTGATACCGGTGTCGGCATGACTGCACAACAACTCGAT
CTGCTATTTGCACCGTTTACCCGCTTCGATGAACCACTCACGCAGCGATCCAGTGGTGTCGGTATCGGTCTGGCCATTGC
TCACCGGTTATGTCTCCTAATGAATGGAACGATTGCCGTTACCAGTCAGCCCGGTAGCGGTACAACGTTCTTGATTACGC
TTCCCCTGCCTTCTGATCAGACTGACACCCTGAGTGACACCTATGACTACCAAACTGAACTCAATGCGTCTTCTTGA

Upstream 100 bases:

>100_bases
CGGATAGAGGGCACCCTTGAAACGTTCCAGAAGGCTGCTGACACAGTGGTTGCGGTTGCCTGTGGCTAAACGGCTCCTCA
TTGCAGGAACGCCGTTCATT

Downstream 100 bases:

>100_bases
ACGTCATCACATCGCGTACACTGTGCATGAGTACGAATACAACGATGCATACGACGCGCAAACAATTGCCCGGATGATCG
GGGTGCCGGCAAGTCAGGTA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MLFLVYGVVWEYRGWGVVLTILTIILMIGLWQYFQHRITIQQQRIRELEQELADAQAVAALATRTKSAFLTNISHELRTP
LNTIIGYSELLHEDVLDERLAVDEVRERLERIQAAARQLLSLINDIIELARLEAGDVPLNYEPWPIGLLIKEVCATIEPL
VEQQQNRLVVDVQPDPEMVVHIDRKKVRQVLLHLLDNAVKFTRQGEIKFIVTIRRSDDGTELLICNISDTGVGMTAQQLD
LLFAPFTRFDEPLTQRSSGVGIGLAIAHRLCLLMNGTIAVTSQPGSGTTFLITLPLPSDQTDTLSDTYDYQTELNASS

Sequences:

>Translated_318_residues
MLFLVYGVVWEYRGWGVVLTILTIILMIGLWQYFQHRITIQQQRIRELEQELADAQAVAALATRTKSAFLTNISHELRTP
LNTIIGYSELLHEDVLDERLAVDEVRERLERIQAAARQLLSLINDIIELARLEAGDVPLNYEPWPIGLLIKEVCATIEPL
VEQQQNRLVVDVQPDPEMVVHIDRKKVRQVLLHLLDNAVKFTRQGEIKFIVTIRRSDDGTELLICNISDTGVGMTAQQLD
LLFAPFTRFDEPLTQRSSGVGIGLAIAHRLCLLMNGTIAVTSQPGSGTTFLITLPLPSDQTDTLSDTYDYQTELNASS
>Mature_318_residues
MLFLVYGVVWEYRGWGVVLTILTIILMIGLWQYFQHRITIQQQRIRELEQELADAQAVAALATRTKSAFLTNISHELRTP
LNTIIGYSELLHEDVLDERLAVDEVRERLERIQAAARQLLSLINDIIELARLEAGDVPLNYEPWPIGLLIKEVCATIEPL
VEQQQNRLVVDVQPDPEMVVHIDRKKVRQVLLHLLDNAVKFTRQGEIKFIVTIRRSDDGTELLICNISDTGVGMTAQQLD
LLFAPFTRFDEPLTQRSSGVGIGLAIAHRLCLLMNGTIAVTSQPGSGTTFLITLPLPSDQTDTLSDTYDYQTELNASS

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=34.5098039215686, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI48994928, Length=254, Percent_Identity=36.2204724409449, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=33.0708661417323, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87081816, Length=254, Percent_Identity=32.6771653543307, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788713, Length=251, Percent_Identity=31.0756972111554, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=29.8701298701299, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790436, Length=299, Percent_Identity=25.0836120401338, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786600, Length=234, Percent_Identity=26.4957264957265, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790346, Length=233, Percent_Identity=29.1845493562232, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788549, Length=257, Percent_Identity=28.0155642023346, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786783, Length=235, Percent_Identity=28.5106382978723, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=25.6505576208178, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787894, Length=233, Percent_Identity=25.7510729613734, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 35827; Mature: 35827

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLVYGVVWEYRGWGVVLTILTIILMIGLWQYFQHRITIQQQRIRELEQELADAQAVAA
CEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATRTKSAFLTNISHELRTPLNTIIGYSELLHEDVLDERLAVDEVRERLERIQAAARQLL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLINDIIELARLEAGDVPLNYEPWPIGLLIKEVCATIEPLVEQQQNRLVVDVQPDPEMVV
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEE
HIDRKKVRQVLLHLLDNAVKFTRQGEIKFIVTIRRSDDGTELLICNISDTGVGMTAQQLD
EECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHH
LLFAPFTRFDEPLTQRSSGVGIGLAIAHRLCLLMNGTIAVTSQPGSGTTFLITLPLPSDQ
HHHHCHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEECCCCC
TDTLSDTYDYQTELNASS
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLFLVYGVVWEYRGWGVVLTILTIILMIGLWQYFQHRITIQQQRIRELEQELADAQAVAA
CEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATRTKSAFLTNISHELRTPLNTIIGYSELLHEDVLDERLAVDEVRERLERIQAAARQLL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLINDIIELARLEAGDVPLNYEPWPIGLLIKEVCATIEPLVEQQQNRLVVDVQPDPEMVV
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEE
HIDRKKVRQVLLHLLDNAVKFTRQGEIKFIVTIRRSDDGTELLICNISDTGVGMTAQQLD
EECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHH
LLFAPFTRFDEPLTQRSSGVGIGLAIAHRLCLLMNGTIAVTSQPGSGTTFLITLPLPSDQ
HHHHCHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEECCCCC
TDTLSDTYDYQTELNASS
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]