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The map label for this gene is ecoRVM [H]

Identifier: 222526839

GI number: 222526839

Start: 4496735

End: 4497658

Strand: Reverse

Name: ecoRVM [H]

Synonym: Chy400_3614

Alternate gene names: 222526839

Gene position: 4497658-4496735 (Counterclockwise)

Preceding gene: 222526841

Following gene: 222526838

Centisome position: 85.36

GC content: 39.07

Gene sequence:

>924_bases
ATGAACACTAGAGGTTTACCACGTACAGTACAACGAGTCATTGTTCCACCAATAAAGTGTCAAGGAATTAAGACTAAACT
GACTAAGTTTATTCTAAGTAATATCTCCTGGCACGGTCAGGGACGATGGATTGAGCCATTTTTAGGTTCAGGAGCTGTAC
TTTTCAATGTTCAGCCCGATTATGCAATTGTAAATGACATCAATCCTCATATCATACGCCTGTATCAGATGATATATGAA
GGAAAAATATTTCCTGAAACTGTACGAAAGTATTTGACAGAAGAAGGAAAGAAGCTATTATTAAAAGGCGAAGATTACTA
CTACTTTGTCAGGGAACGTTTTAACAAAAGTGGTGATCCCTTGGATTTTATTTTCTTGAATCGGTCATGTTTTAACGGTG
TCATGCGTTTTAATAAAAAAGGTGAATTTAATGTTCCGTTTTGTCGAAAGACAGATCGCTTTCGTCAGGCATATATCACG
AAAATCGTTAATCAAATAACTCAGATTCGTAATATTATGAAAGGCAAAGCGTGGGAGTTTCGGATTGGAGATTGGCGAGA
TTGCCTGCACCATGTTACGCCGGACGACTTTGTCTACCTTGATCCGCCTTACATTGGCAGACATACAGATTACTATCAAC
AATGGACTGAACATGATGCGGCTGAGTTAGCAACGGTGACGCAGCATCTGCCTTGTGGCTTTGCGTTGTCTATGTGGAAA
GAAAACCGATATCGTCAAAATCAGCACATCGCTCAATATTGGAACGGATTGATCGAGCGGACAGTTTCTCATTTTTATCA
TGTAGGTTCGACAGAAGACTTACGAAATGAGATGGAAGAAGCATTATTGATAAAACCAGGCTTTGCAACAGCAATTACAC
CATCAGTAGAATCTGACAAACCACTACAATTGACATTTGTATAA

Upstream 100 bases:

>100_bases
TGGATGTTTTACCTGACTAAAGATATGGCAAGAGCAGCAGAATTGGTCAGACCACCATATACAGATCTACGAAGCTATTT
GCGGTATAAAGGTTTACAAT

Downstream 100 bases:

>100_bases
GCAATAAATACCATAACAGAGATAGGTGTATCTGCGTGCATTAAGCGTTGTACATTGCATCACCACGTCCACAGCATCGC
TGGATCGTATTCATTACCCA

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.EcoRV; Adenine-specific methyltransferase EcoRV [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNTRGLPRTVQRVIVPPIKCQGIKTKLTKFILSNISWHGQGRWIEPFLGSGAVLFNVQPDYAIVNDINPHIIRLYQMIYE
GKIFPETVRKYLTEEGKKLLLKGEDYYYFVRERFNKSGDPLDFIFLNRSCFNGVMRFNKKGEFNVPFCRKTDRFRQAYIT
KIVNQITQIRNIMKGKAWEFRIGDWRDCLHHVTPDDFVYLDPPYIGRHTDYYQQWTEHDAAELATVTQHLPCGFALSMWK
ENRYRQNQHIAQYWNGLIERTVSHFYHVGSTEDLRNEMEEALLIKPGFATAITPSVESDKPLQLTFV

Sequences:

>Translated_307_residues
MNTRGLPRTVQRVIVPPIKCQGIKTKLTKFILSNISWHGQGRWIEPFLGSGAVLFNVQPDYAIVNDINPHIIRLYQMIYE
GKIFPETVRKYLTEEGKKLLLKGEDYYYFVRERFNKSGDPLDFIFLNRSCFNGVMRFNKKGEFNVPFCRKTDRFRQAYIT
KIVNQITQIRNIMKGKAWEFRIGDWRDCLHHVTPDDFVYLDPPYIGRHTDYYQQWTEHDAAELATVTQHLPCGFALSMWK
ENRYRQNQHIAQYWNGLIERTVSHFYHVGSTEDLRNEMEEALLIKPGFATAITPSVESDKPLQLTFV
>Mature_307_residues
MNTRGLPRTVQRVIVPPIKCQGIKTKLTKFILSNISWHGQGRWIEPFLGSGAVLFNVQPDYAIVNDINPHIIRLYQMIYE
GKIFPETVRKYLTEEGKKLLLKGEDYYYFVRERFNKSGDPLDFIFLNRSCFNGVMRFNKKGEFNVPFCRKTDRFRQAYIT
KIVNQITQIRNIMKGKAWEFRIGDWRDCLHHVTPDDFVYLDPPYIGRHTDYYQQWTEHDAAELATVTQHLPCGFALSMWK
ENRYRQNQHIAQYWNGLIERTVSHFYHVGSTEDLRNEMEEALLIKPGFATAITPSVESDKPLQLTFV

Specific function: This methylase recognizes the double-stranded sequence GATATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the EcoRV endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=268, Percent_Identity=27.9850746268657, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 36139; Mature: 36139

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRGLPRTVQRVIVPPIKCQGIKTKLTKFILSNISWHGQGRWIEPFLGSGAVLFNVQPD
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECHHCCCCEEEEEECCC
YAIVNDINPHIIRLYQMIYEGKIFPETVRKYLTEEGKKLLLKGEDYYYFVRERFNKSGDP
EEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEHHHCCCCCCC
LDFIFLNRSCFNGVMRFNKKGEFNVPFCRKTDRFRQAYITKIVNQITQIRNIMKGKAWEF
EEEEEECHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
RIGDWRDCLHHVTPDDFVYLDPPYIGRHTDYYQQWTEHDAAELATVTQHLPCGFALSMWK
ECCCHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ENRYRQNQHIAQYWNGLIERTVSHFYHVGSTEDLRNEMEEALLIKPGFATAITPSVESDK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCEECCCCCCCC
PLQLTFV
CEEEEEC
>Mature Secondary Structure
MNTRGLPRTVQRVIVPPIKCQGIKTKLTKFILSNISWHGQGRWIEPFLGSGAVLFNVQPD
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECHHCCCCEEEEEECCC
YAIVNDINPHIIRLYQMIYEGKIFPETVRKYLTEEGKKLLLKGEDYYYFVRERFNKSGDP
EEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEHHHCCCCCCC
LDFIFLNRSCFNGVMRFNKKGEFNVPFCRKTDRFRQAYITKIVNQITQIRNIMKGKAWEF
EEEEEECHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
RIGDWRDCLHHVTPDDFVYLDPPYIGRHTDYYQQWTEHDAAELATVTQHLPCGFALSMWK
ECCCHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ENRYRQNQHIAQYWNGLIERTVSHFYHVGSTEDLRNEMEEALLIKPGFATAITPSVESDK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCEECCCCCCCC
PLQLTFV
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6328432 [H]