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The map label for this gene is glpK [H]

Identifier: 222526778

GI number: 222526778

Start: 4425295

End: 4426791

Strand: Reverse

Name: glpK [H]

Synonym: Chy400_3552

Alternate gene names: 222526778

Gene position: 4426791-4425295 (Counterclockwise)

Preceding gene: 222526779

Following gene: 222526777

Centisome position: 84.02

GC content: 57.65

Gene sequence:

>1497_bases
ATGGCAAAGTACGCCGCTGCTATCGACCAGGGCACAACCAGTACCCGCTGTATGATCTTCGACCATAGCGGGAATGTCAT
TTGCTACGATCAGAAAGAACACGAGCAGATTTATCCCCGCCCCGGCTGGGTAGAACACAGCCCTGATGAGATTTGGGAGC
GTACCCAAAGTGTGATTCGTGGGGCACTAAGCAAAGGCGGCCTGAGCGCAAGCGATATTGTCGCCGTCGGCATTACCAAT
CAGCGCGAGACAACGGTGGTCTGGAATCGCAAAACAGGCCGACCGGTGTACAATGCGATAGTCTGGCAAGACACCCGCAC
CGACCAGATTTGTAATGAACTTGCCGCCGACGGCGGTCAGGATCGGTTCCGCCCCAAGGTTGGCTTGCCACTGGCAACCT
ACTTCTCCGGTCCCAAGATTCGCTGGATTCTCGACAATGTGCCCGGCGCCCGTGAGGCGGCGGAGGCCGGTGATGTGGTG
TTTGGTAACATCGATACCTTCCTGACCTGGTGGCTCACCGGTGGGCCCAACGGTGGTGTCCACGTTACCGATGTGACCAA
CGCCTCACGCACGATGTTGATGAATCTCGAAACCCTTGATTGGGATGATGAGATTCTCGGCATTATGGGGATTCCGCGCC
AGATGCTGCCCAAGATCGTACCCTCCAGCATGGTTTACGGTACGGCAACAGGTGAACTGGCCGGTGTGCCGGTGGCCGGT
ATCCTGGGCGATCAGCAGGCGGCTATGGTCGGACAAACCTGCTTCGATGTCGGTGAGGCCAAAAATACCTACGGTACCGG
TTCGTTTATGCTGCTCAACACCGGCACGAAACTGGTTCCATCCAAGAGTGGCCTGCTGACCACCGTCTGTTACAAGTTCG
GCGATCAACCCGCCGTCTACGCCCTCGAAGGCTCAATCGCTATCACCGGTGCTCTGGTGCAGTGGCTCCGCGATAACCTC
GGTCTGATTACATCCTCGGCAGAAGTCGAGGCGCTGGCAAATCTGGTCGAAGATAACGGCGGTATCTACTTCGTGCCGGC
CTTCTCTGGACTCTTCGCACCCTACTGGCGCTCGGACGCCCGTGGTGTGATTGTCGGCCTGACTCGTTATGTCAACAAAG
GCCACCTCGCCCGCGCCGTGCTCGAAGCCACTGCCTACCAGACGCGCGAGGTGCTCGACGCGATGGAGCAGGACTCAGGC
GTCAAGCTGACCGCCTTGAAGGTCGACGGTGGTATGGTGTACAATAATACCCTGATGCAATTCCAGGCCGATATTTTGGG
TGTGCCCGTTATTCGGCCCAAGGTTGCAGAGACCACATCGCTCGGTGCTGCCTATGCAGCCGGTTTAGCAGTCGGCTTCT
GGTCAAATACCGATGAGATGCGGGCCAACTGGGGTGTTGATCACACCTGGACACCACAGATGGATGAAGCAACCCGCGAG
CGGCTCTATCGGGGCTGGAAGAAGGCAGTTACCCGTACCTTCGATTGGGTGGAGTAA

Upstream 100 bases:

>100_bases
TCTGATGGCGCAGGCGCTGCTTGAGGTAGTCGAACAGGCTGCCAAAGGGTGATGTCGTTCCAACCGGATCGTGTGAGCGT
AGACAAAGGAGTCTTCAATT

Downstream 100 bases:

>100_bases
ACCACCCTCACCGCACCCGAACAGACCATTGTGGCAAGACCGGCGGGACATCTCCTGCCGGTCTTGCTGCCCGTCAGGAG
TTCATTATGCAGACTATCTC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MAKYAAAIDQGTTSTRCMIFDHSGNVICYDQKEHEQIYPRPGWVEHSPDEIWERTQSVIRGALSKGGLSASDIVAVGITN
QRETTVVWNRKTGRPVYNAIVWQDTRTDQICNELAADGGQDRFRPKVGLPLATYFSGPKIRWILDNVPGAREAAEAGDVV
FGNIDTFLTWWLTGGPNGGVHVTDVTNASRTMLMNLETLDWDDEILGIMGIPRQMLPKIVPSSMVYGTATGELAGVPVAG
ILGDQQAAMVGQTCFDVGEAKNTYGTGSFMLLNTGTKLVPSKSGLLTTVCYKFGDQPAVYALEGSIAITGALVQWLRDNL
GLITSSAEVEALANLVEDNGGIYFVPAFSGLFAPYWRSDARGVIVGLTRYVNKGHLARAVLEATAYQTREVLDAMEQDSG
VKLTALKVDGGMVYNNTLMQFQADILGVPVIRPKVAETTSLGAAYAAGLAVGFWSNTDEMRANWGVDHTWTPQMDEATRE
RLYRGWKKAVTRTFDWVE

Sequences:

>Translated_498_residues
MAKYAAAIDQGTTSTRCMIFDHSGNVICYDQKEHEQIYPRPGWVEHSPDEIWERTQSVIRGALSKGGLSASDIVAVGITN
QRETTVVWNRKTGRPVYNAIVWQDTRTDQICNELAADGGQDRFRPKVGLPLATYFSGPKIRWILDNVPGAREAAEAGDVV
FGNIDTFLTWWLTGGPNGGVHVTDVTNASRTMLMNLETLDWDDEILGIMGIPRQMLPKIVPSSMVYGTATGELAGVPVAG
ILGDQQAAMVGQTCFDVGEAKNTYGTGSFMLLNTGTKLVPSKSGLLTTVCYKFGDQPAVYALEGSIAITGALVQWLRDNL
GLITSSAEVEALANLVEDNGGIYFVPAFSGLFAPYWRSDARGVIVGLTRYVNKGHLARAVLEATAYQTREVLDAMEQDSG
VKLTALKVDGGMVYNNTLMQFQADILGVPVIRPKVAETTSLGAAYAAGLAVGFWSNTDEMRANWGVDHTWTPQMDEATRE
RLYRGWKKAVTRTFDWVE
>Mature_497_residues
AKYAAAIDQGTTSTRCMIFDHSGNVICYDQKEHEQIYPRPGWVEHSPDEIWERTQSVIRGALSKGGLSASDIVAVGITNQ
RETTVVWNRKTGRPVYNAIVWQDTRTDQICNELAADGGQDRFRPKVGLPLATYFSGPKIRWILDNVPGAREAAEAGDVVF
GNIDTFLTWWLTGGPNGGVHVTDVTNASRTMLMNLETLDWDDEILGIMGIPRQMLPKIVPSSMVYGTATGELAGVPVAGI
LGDQQAAMVGQTCFDVGEAKNTYGTGSFMLLNTGTKLVPSKSGLLTTVCYKFGDQPAVYALEGSIAITGALVQWLRDNLG
LITSSAEVEALANLVEDNGGIYFVPAFSGLFAPYWRSDARGVIVGLTRYVNKGHLARAVLEATAYQTREVLDAMEQDSGV
KLTALKVDGGMVYNNTLMQFQADILGVPVIRPKVAETTSLGAAYAAGLAVGFWSNTDEMRANWGVDHTWTPQMDEATRER
LYRGWKKAVTRTFDWVE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=501, Percent_Identity=50.8982035928144, Blast_Score=499, Evalue=1e-141,
Organism=Homo sapiens, GI4504007, Length=501, Percent_Identity=50.8982035928144, Blast_Score=499, Evalue=1e-141,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=50.2958579881657, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI41393575, Length=501, Percent_Identity=49.7005988023952, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI88196792, Length=511, Percent_Identity=32.6810176125245, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1790361, Length=500, Percent_Identity=54.6, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1790005, Length=502, Percent_Identity=25.4980079681275, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1789987, Length=501, Percent_Identity=24.5508982035928, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787789, Length=503, Percent_Identity=25.844930417495, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=45.8333333333333, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71986140, Length=524, Percent_Identity=25.5725190839695, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI71986147, Length=302, Percent_Identity=29.1390728476821, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321755, Length=552, Percent_Identity=39.8550724637681, Blast_Score=353, Evalue=4e-98,
Organism=Drosophila melanogaster, GI17864214, Length=505, Percent_Identity=47.1287128712871, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24654573, Length=450, Percent_Identity=48.4444444444444, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655555, Length=502, Percent_Identity=43.2270916334661, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24655560, Length=502, Percent_Identity=43.2270916334661, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24655570, Length=502, Percent_Identity=43.2270916334661, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24655574, Length=502, Percent_Identity=43.2270916334661, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24655565, Length=502, Percent_Identity=43.2270916334661, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=36, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=36.4562118126273, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=28.7619047619048, Blast_Score=222, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24656367, Length=510, Percent_Identity=29.4117647058824, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24656372, Length=329, Percent_Identity=32.2188449848024, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI45552909, Length=270, Percent_Identity=31.1111111111111, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24657106, Length=491, Percent_Identity=24.2362525458248, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24657102, Length=491, Percent_Identity=24.2362525458248, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54325; Mature: 54194

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYAAAIDQGTTSTRCMIFDHSGNVICYDQKEHEQIYPRPGWVEHSPDEIWERTQSVIR
CCCHHHHHCCCCCCEEEEEEECCCCEEEECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
GALSKGGLSASDIVAVGITNQRETTVVWNRKTGRPVYNAIVWQDTRTDQICNELAADGGQ
HHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCCC
DRFRPKVGLPLATYFSGPKIRWILDNVPGAREAAEAGDVVFGNIDTFLTWWLTGGPNGGV
CCCCCCCCCEEHHHCCCCEEEEEECCCCCHHHHHHCCCEEEECHHEEEEEEEECCCCCCE
HVTDVTNASRTMLMNLETLDWDDEILGIMGIPRQMLPKIVPSSMVYGTATGELAGVPVAG
EEEEECCCCEEEEEEEEECCCCCHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCCCEEE
ILGDQQAAMVGQTCFDVGEAKNTYGTGSFMLLNTGTKLVPSKSGLLTTVCYKFGDQPAVY
ECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHCCCCEEE
ALEGSIAITGALVQWLRDNLGLITSSAEVEALANLVEDNGGIYFVPAFSGLFAPYWRSDA
EEECCEEHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCC
RGVIVGLTRYVNKGHLARAVLEATAYQTREVLDAMEQDSGVKLTALKVDGGMVYNNTLMQ
CEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCHHHH
FQADILGVPVIRPKVAETTSLGAAYAAGLAVGFWSNTDEMRANWGVDHTWTPQMDEATRE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHH
RLYRGWKKAVTRTFDWVE
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKYAAAIDQGTTSTRCMIFDHSGNVICYDQKEHEQIYPRPGWVEHSPDEIWERTQSVIR
CCHHHHHCCCCCCEEEEEEECCCCEEEECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
GALSKGGLSASDIVAVGITNQRETTVVWNRKTGRPVYNAIVWQDTRTDQICNELAADGGQ
HHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCCC
DRFRPKVGLPLATYFSGPKIRWILDNVPGAREAAEAGDVVFGNIDTFLTWWLTGGPNGGV
CCCCCCCCCEEHHHCCCCEEEEEECCCCCHHHHHHCCCEEEECHHEEEEEEEECCCCCCE
HVTDVTNASRTMLMNLETLDWDDEILGIMGIPRQMLPKIVPSSMVYGTATGELAGVPVAG
EEEEECCCCEEEEEEEEECCCCCHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCCCCEEE
ILGDQQAAMVGQTCFDVGEAKNTYGTGSFMLLNTGTKLVPSKSGLLTTVCYKFGDQPAVY
ECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEECCCCCCHHHHHHHHHCCCCEEE
ALEGSIAITGALVQWLRDNLGLITSSAEVEALANLVEDNGGIYFVPAFSGLFAPYWRSDA
EEECCEEHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCC
RGVIVGLTRYVNKGHLARAVLEATAYQTREVLDAMEQDSGVKLTALKVDGGMVYNNTLMQ
CEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCHHHH
FQADILGVPVIRPKVAETTSLGAAYAAGLAVGFWSNTDEMRANWGVDHTWTPQMDEATRE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHH
RLYRGWKKAVTRTFDWVE
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA