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The map label for this gene is glpA [H]

Identifier: 222526777

GI number: 222526777

Start: 4423538

End: 4425208

Strand: Reverse

Name: glpA [H]

Synonym: Chy400_3551

Alternate gene names: 222526777

Gene position: 4425208-4423538 (Counterclockwise)

Preceding gene: 222526778

Following gene: 222526776

Centisome position: 83.99

GC content: 56.73

Gene sequence:

>1671_bases
ATGCAGACTATCTCCACCGATGTGCTGGTTATTGGTGGCGGCGCTACCGGCACCGGTTGCGCACTCGATCTGGCAATGCG
CGGTATCCGTTGTGTGCTGGTAGAAAAGGGCGATTTGACACACGGTACCACCGGTCGCTATCATGGCCTGCTCCACTCAG
GTGGGCGCTACGTAACCAAAGACCCCCAGTCAGCCAGCGAATGCGCCCACGAAAATGCGATTTTGCGGCGGATCATGCCG
CATTGTATCGAGCAAACCTCTGGCTTCTTTGTGATTACACCGTGGGACGATGAGAGCTATGGCGATGTCTTCGTCGCCAA
CTGCCATCGTACCGGTGTACCGGTCCAGGAGATCAGCGTCGCCGAGATGCTGCGTCGTGAACCACTCCTTAACCCCCGTA
TTTCCCGCGTCTTTGAAGTTCCCGACGGCTCTGCCGACTCGTTCCTTGCTACCCATTCGGTCGCACTGGCGGCACGTGAA
TATGGTGCTGATATTCGCACCTACCATCATGTTGTCGAACTCATTCGGGTCGGGGATCGCGTCGTAGGCGCAATCGCCGA
GGATTTACGTAGCGGTGAACGGGTGCGGATCGACTGCGCGTATGTGCTCAACGCGGCAGGTGCCTGGGCCGGGAAGATTG
CCGCCCTGGCCGGTCTAACGGTGAATGTCGTGCCCGGTAAAGGTACCATGGTTGCGATGAACCACCGTATGGTGAATACT
GTGGTCAATCGCTGTAAGCTGCCGTCTGATGGTGACATTATCGTGCCAATTCACACGGTTGCTGTGATCGGTACGACCGA
TGTGCACGTTCCCGATCCCGATGTCTTTGGGATCGAACCCGAAGAGATACAGTTTATGCTCGACGAAGGCGAGAAGCTCA
TTCCCGGCTTCCGTCAGTATCGTGCTTTACGAGCCTGGGCTGGCGTGCGCCCGCTCTACAAAGATAAAGATGTGGCTAGC
GACCGCGATATTCCCCGCACGTTCAAATTACTTGACCACGAAGAACGTGATGGCGTGAGTGGTATCTTCTCGATTGTTGG
CGGTAAATGGACAACCTATCGGCTGATGGCCGAACAAACAGTCAATGAGATTGCTCGCCGCCTGGGTAATACTCAACCCT
GTCGCACGGCAAGCGAGGTCTTGCCGGTGCCCCGTTTCGAGCACAGTAGCCGCGCGACGGTCACTGTTTCACCCAATCCG
GCTGCGCAAACTCCTCACTACTGGTTGGGGAAACCATTGGCGCTCGTTGAGAGTCACCACGCGGCTGGTGATCTGATCTG
TGAATGCGAGCTGGTAACACGCGACCGGATTCAGGCTGCAATTGATGCTGGTGCGCATAATCTTGACGATATTCGCCGCG
ATGTACGTATGGGCATGGGGCCGTGTCAGGGCGGCTGGTGTATCTACCGCACTGCCAACCTGCTCTATGAACAACGCTGT
GCAGCAGGTCTCAGCCATGTTCATACCAACGAAGCGATCATGCACTTCCTCCAGGAACGCTGGAAAGGTCTGACGCCGGT
ATTGTGGGGCGACCAGTTGCGACAGGCACGCCTCGATGAACTGATCTACGTGGGTGTGCTGGGCATTGATCGCCTGCGTA
CACTGGCCGAACCGGGTGGTGGATTGGTGACGCAACACCCGGCAATTGCAACTGAGTATGTGCAGGCATAG

Upstream 100 bases:

>100_bases
ATTGGGTGGAGTAAACCACCCTCACCGCACCCGAACAGACCATTGTGGCAAGACCGGCGGGACATCTCCTGCCGGTCTTG
CTGCCCGTCAGGAGTTCATT

Downstream 100 bases:

>100_bases
GTTGCCGGGAGAGTGATATGTACGATACGATTGTCATTGGTGCCGGCCTGGCCGGCATGATGGCCGCCATCGGGCGTGCC
GAACGGGGGGAAAAAGTTCT

Product: glycerol-3-phosphate dehydrogenase, anaerobic subunit A

Products: NA

Alternate protein names: G-3-P dehydrogenase [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MQTISTDVLVIGGGATGTGCALDLAMRGIRCVLVEKGDLTHGTTGRYHGLLHSGGRYVTKDPQSASECAHENAILRRIMP
HCIEQTSGFFVITPWDDESYGDVFVANCHRTGVPVQEISVAEMLRREPLLNPRISRVFEVPDGSADSFLATHSVALAARE
YGADIRTYHHVVELIRVGDRVVGAIAEDLRSGERVRIDCAYVLNAAGAWAGKIAALAGLTVNVVPGKGTMVAMNHRMVNT
VVNRCKLPSDGDIIVPIHTVAVIGTTDVHVPDPDVFGIEPEEIQFMLDEGEKLIPGFRQYRALRAWAGVRPLYKDKDVAS
DRDIPRTFKLLDHEERDGVSGIFSIVGGKWTTYRLMAEQTVNEIARRLGNTQPCRTASEVLPVPRFEHSSRATVTVSPNP
AAQTPHYWLGKPLALVESHHAAGDLICECELVTRDRIQAAIDAGAHNLDDIRRDVRMGMGPCQGGWCIYRTANLLYEQRC
AAGLSHVHTNEAIMHFLQERWKGLTPVLWGDQLRQARLDELIYVGVLGIDRLRTLAEPGGGLVTQHPAIATEYVQA

Sequences:

>Translated_556_residues
MQTISTDVLVIGGGATGTGCALDLAMRGIRCVLVEKGDLTHGTTGRYHGLLHSGGRYVTKDPQSASECAHENAILRRIMP
HCIEQTSGFFVITPWDDESYGDVFVANCHRTGVPVQEISVAEMLRREPLLNPRISRVFEVPDGSADSFLATHSVALAARE
YGADIRTYHHVVELIRVGDRVVGAIAEDLRSGERVRIDCAYVLNAAGAWAGKIAALAGLTVNVVPGKGTMVAMNHRMVNT
VVNRCKLPSDGDIIVPIHTVAVIGTTDVHVPDPDVFGIEPEEIQFMLDEGEKLIPGFRQYRALRAWAGVRPLYKDKDVAS
DRDIPRTFKLLDHEERDGVSGIFSIVGGKWTTYRLMAEQTVNEIARRLGNTQPCRTASEVLPVPRFEHSSRATVTVSPNP
AAQTPHYWLGKPLALVESHHAAGDLICECELVTRDRIQAAIDAGAHNLDDIRRDVRMGMGPCQGGWCIYRTANLLYEQRC
AAGLSHVHTNEAIMHFLQERWKGLTPVLWGDQLRQARLDELIYVGVLGIDRLRTLAEPGGGLVTQHPAIATEYVQA
>Mature_556_residues
MQTISTDVLVIGGGATGTGCALDLAMRGIRCVLVEKGDLTHGTTGRYHGLLHSGGRYVTKDPQSASECAHENAILRRIMP
HCIEQTSGFFVITPWDDESYGDVFVANCHRTGVPVQEISVAEMLRREPLLNPRISRVFEVPDGSADSFLATHSVALAARE
YGADIRTYHHVVELIRVGDRVVGAIAEDLRSGERVRIDCAYVLNAAGAWAGKIAALAGLTVNVVPGKGTMVAMNHRMVNT
VVNRCKLPSDGDIIVPIHTVAVIGTTDVHVPDPDVFGIEPEEIQFMLDEGEKLIPGFRQYRALRAWAGVRPLYKDKDVAS
DRDIPRTFKLLDHEERDGVSGIFSIVGGKWTTYRLMAEQTVNEIARRLGNTQPCRTASEVLPVPRFEHSSRATVTVSPNP
AAQTPHYWLGKPLALVESHHAAGDLICECELVTRDRIQAAIDAGAHNLDDIRRDVRMGMGPCQGGWCIYRTANLLYEQRC
AAGLSHVHTNEAIMHFLQERWKGLTPVLWGDQLRQARLDELIYVGVLGIDRLRTLAEPGGGLVTQHPAIATEYVQA

Specific function: Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor [H]

COG id: COG0578

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI285002233, Length=399, Percent_Identity=30.3258145363409, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI285002231, Length=399, Percent_Identity=30.3258145363409, Blast_Score=146, Evalue=5e-35,
Organism=Escherichia coli, GI1788574, Length=515, Percent_Identity=41.3592233009709, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI2367226, Length=367, Percent_Identity=23.433242506812, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17509253, Length=388, Percent_Identity=28.8659793814433, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115534726, Length=403, Percent_Identity=28.5359801488834, Blast_Score=92, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322036, Length=442, Percent_Identity=30.0904977375566, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24586295, Length=401, Percent_Identity=28.927680798005, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161077125, Length=401, Percent_Identity=29.426433915212, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24653942, Length=401, Percent_Identity=29.426433915212, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24653944, Length=401, Percent_Identity=29.426433915212, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI20130025, Length=401, Percent_Identity=29.426433915212, Blast_Score=106, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR006076
- InterPro:   IPR017752
- InterPro:   IPR000447 [H]

Pfam domain/function: PF01266 DAO; PF04324 Fer2_BFD [H]

EC number: =1.1.5.3 [H]

Molecular weight: Translated: 61010; Mature: 61010

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00977 FAD_G3PDH_1 ; PS00978 FAD_G3PDH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTISTDVLVIGGGATGTGCALDLAMRGIRCVLVEKGDLTHGTTGRYHGLLHSGGRYVTK
CCCCCCCEEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHEECCCEEEEC
DPQSASECAHENAILRRIMPHCIEQTSGFFVITPWDDESYGDVFVANCHRTGVPVQEISV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHH
AEMLRREPLLNPRISRVFEVPDGSADSFLATHSVALAAREYGADIRTYHHVVELIRVGDR
HHHHHHCCCCCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VVGAIAEDLRSGERVRIDCAYVLNAAGAWAGKIAALAGLTVNVVPGKGTMVAMNHRMVNT
HHHHHHHHHCCCCEEEEEEHHHHHCCCCHHHHHHHHHCCEEEEECCCCCEEEECHHHHHH
VVNRCKLPSDGDIIVPIHTVAVIGTTDVHVPDPDVFGIEPEEIQFMLDEGEKLIPGFRQY
HHHHCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHEEECCCCCCCCHHHHH
RALRAWAGVRPLYKDKDVASDRDIPRTFKLLDHEERDGVSGIFSIVGGKWTTYRLMAEQT
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEEEHHHHHHH
VNEIARRLGNTQPCRTASEVLPVPRFEHSSRATVTVSPNPAAQTPHYWLGKPLALVESHH
HHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHCCCCHHHHHCCC
AAGDLICECELVTRDRIQAAIDAGAHNLDDIRRDVRMGMGPCQGGWCIYRTANLLYEQRC
CCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHH
AAGLSHVHTNEAIMHFLQERWKGLTPVLWGDQLRQARLDELIYVGVLGIDRLRTLAEPGG
HHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCC
GLVTQHPAIATEYVQA
CCEECCCCHHHHHHCC
>Mature Secondary Structure
MQTISTDVLVIGGGATGTGCALDLAMRGIRCVLVEKGDLTHGTTGRYHGLLHSGGRYVTK
CCCCCCCEEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHEECCCEEEEC
DPQSASECAHENAILRRIMPHCIEQTSGFFVITPWDDESYGDVFVANCHRTGVPVQEISV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHH
AEMLRREPLLNPRISRVFEVPDGSADSFLATHSVALAAREYGADIRTYHHVVELIRVGDR
HHHHHHCCCCCCCHHHEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VVGAIAEDLRSGERVRIDCAYVLNAAGAWAGKIAALAGLTVNVVPGKGTMVAMNHRMVNT
HHHHHHHHHCCCCEEEEEEHHHHHCCCCHHHHHHHHHCCEEEEECCCCCEEEECHHHHHH
VVNRCKLPSDGDIIVPIHTVAVIGTTDVHVPDPDVFGIEPEEIQFMLDEGEKLIPGFRQY
HHHHCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHEEECCCCCCCCHHHHH
RALRAWAGVRPLYKDKDVASDRDIPRTFKLLDHEERDGVSGIFSIVGGKWTTYRLMAEQT
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCEEEHHHHHHH
VNEIARRLGNTQPCRTASEVLPVPRFEHSSRATVTVSPNPAAQTPHYWLGKPLALVESHH
HHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHCCCCHHHHHCCC
AAGDLICECELVTRDRIQAAIDAGAHNLDDIRRDVRMGMGPCQGGWCIYRTANLLYEQRC
CCCCEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHH
AAGLSHVHTNEAIMHFLQERWKGLTPVLWGDQLRQARLDELIYVGVLGIDRLRTLAEPGG
HHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCC
GLVTQHPAIATEYVQA
CCEECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]