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The map label for this gene is phbC [H]

Identifier: 222526747

GI number: 222526747

Start: 4385311

End: 4386417

Strand: Reverse

Name: phbC [H]

Synonym: Chy400_3521

Alternate gene names: 222526747

Gene position: 4386417-4385311 (Counterclockwise)

Preceding gene: 222526748

Following gene: 222526746

Centisome position: 83.25

GC content: 53.3

Gene sequence:

>1107_bases
ATGACCAGTTCTGATGCCGCGCCAATCGATCTTGAGCGGATGACCGAGCAGTTGCTGAAAGAGATCGAGCGCACAACGAA
GAGCAGTGACATGGCTGCTCGCATCGCTACCAATCGTATCCGAGTTGCTGTCGGTCAGACGCCCAAAGAGCAGATCTGGG
CGTTGAACAAGATGCGTCTTTACCATTACTACCCGCAAGTACCACCGGAGCAGCGCAAGCCGGTACCGATCTTGCTCGTC
TTCGCCTTGATCAATCGACCGTATATCTTCGATCTGCGCCCCGGCAATAGCTTCGTAGAATACCTGCTCCAGCACGGCTA
TGAAGTCTATCTGCTCGATTGGGGCCGTCCAGGCTTGGAAGATGCCCATTACGATTTTGAAGATTATTCGCTGAAGTTCC
TGCCACGGGCCATTCGGGCGATGCAGCGGCATAGCGGTAAGCGTGAGTTCTCGATGCTCGGCTGGTGTATCGGAGCCACG
ATTGTCACAATTTACGCGGCGATGCGCCCCGATGATGGCCTGCGCAATTTGATCCTGCTTACCGCACCGCTCGATTTCAG
CGACAAAGAGGGAAGTACCTTCAGTCGCTGGCTCAATACCGAGTATTACAACGTTGATGAACTGGTTCGCCAGTTTGGAA
ATGTCCCGGCAGCCGTGATCGAATACGGCAACAAGATGCTGAAGCCGGTTGAGAACTATATCGGCACCTACCTCAAGCTA
TGGGACAACCTCGACAACCCGGCAGTCGTCGAGGCATGGCTGGCGATGAACACGTGGGTGACCGATAACGTCGACTTTCC
GGGTGCTGCTTTCCGTCAGTGGGTCGTGGAATTTTTCCGCGAAAACCGCTTGATGGAGAACAGGCTTATGATGGAAGGGC
GTGTGGTTGATGTAGCGAATATCAGGGCTAATCTCTTGAATGTGATTGCCGATAAAGATCACATTGTGCCCAACTGTCAA
TCACGCTCAATCATGGATCGGGTCGGAAGTAAAGATAAGAAATTGATCGAATTGAAAGGTGGCCATATCGGTATTATGGT
CGGCAGTGGCGCCAGCAAACGTGCCTGGCCCCTGATGGAAGCCTGGTTGAGCGAGCGTAGCACCTGA

Upstream 100 bases:

>100_bases
GCGGGGTATTGAGGAACAACTGACCGCTCTTGCCGCCGTCCGTCCCTCCGGTCGTATCCGCAAGGCAACGACTGAAGACA
AAGAGTAGGAGTGTTTGATC

Downstream 100 bases:

>100_bases
CATCCCCTTATTTCGTTGATACGCACAGCGGTTGACATCGTCATCAGTAATGCTGACGATGCACCGTTTTGTGCTGTCAA
GTTTTCATGATAGCCGGTCT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTSSDAAPIDLERMTEQLLKEIERTTKSSDMAARIATNRIRVAVGQTPKEQIWALNKMRLYHYYPQVPPEQRKPVPILLV
FALINRPYIFDLRPGNSFVEYLLQHGYEVYLLDWGRPGLEDAHYDFEDYSLKFLPRAIRAMQRHSGKREFSMLGWCIGAT
IVTIYAAMRPDDGLRNLILLTAPLDFSDKEGSTFSRWLNTEYYNVDELVRQFGNVPAAVIEYGNKMLKPVENYIGTYLKL
WDNLDNPAVVEAWLAMNTWVTDNVDFPGAAFRQWVVEFFRENRLMENRLMMEGRVVDVANIRANLLNVIADKDHIVPNCQ
SRSIMDRVGSKDKKLIELKGGHIGIMVGSGASKRAWPLMEAWLSERST

Sequences:

>Translated_368_residues
MTSSDAAPIDLERMTEQLLKEIERTTKSSDMAARIATNRIRVAVGQTPKEQIWALNKMRLYHYYPQVPPEQRKPVPILLV
FALINRPYIFDLRPGNSFVEYLLQHGYEVYLLDWGRPGLEDAHYDFEDYSLKFLPRAIRAMQRHSGKREFSMLGWCIGAT
IVTIYAAMRPDDGLRNLILLTAPLDFSDKEGSTFSRWLNTEYYNVDELVRQFGNVPAAVIEYGNKMLKPVENYIGTYLKL
WDNLDNPAVVEAWLAMNTWVTDNVDFPGAAFRQWVVEFFRENRLMENRLMMEGRVVDVANIRANLLNVIADKDHIVPNCQ
SRSIMDRVGSKDKKLIELKGGHIGIMVGSGASKRAWPLMEAWLSERST
>Mature_367_residues
TSSDAAPIDLERMTEQLLKEIERTTKSSDMAARIATNRIRVAVGQTPKEQIWALNKMRLYHYYPQVPPEQRKPVPILLVF
ALINRPYIFDLRPGNSFVEYLLQHGYEVYLLDWGRPGLEDAHYDFEDYSLKFLPRAIRAMQRHSGKREFSMLGWCIGATI
VTIYAAMRPDDGLRNLILLTAPLDFSDKEGSTFSRWLNTEYYNVDELVRQFGNVPAAVIEYGNKMLKPVENYIGTYLKLW
DNLDNPAVVEAWLAMNTWVTDNVDFPGAAFRQWVVEFFRENRLMENRLMMEGRVVDVANIRANLLNVIADKDHIVPNCQS
RSIMDRVGSKDKKLIELKGGHIGIMVGSGASKRAWPLMEAWLSERST

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010125 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 42356; Mature: 42225

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00019 ACTININ_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSDAAPIDLERMTEQLLKEIERTTKSSDMAARIATNRIRVAVGQTPKEQIWALNKMRL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEEECCCCHHHHHHHHHHEE
YHYYPQVPPEQRKPVPILLVFALINRPYIFDLRPGNSFVEYLLQHGYEVYLLDWGRPGLE
EEECCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCC
DAHYDFEDYSLKFLPRAIRAMQRHSGKREFSMLGWCIGATIVTIYAAMRPDDGLRNLILL
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
TAPLDFSDKEGSTFSRWLNTEYYNVDELVRQFGNVPAAVIEYGNKMLKPVENYIGTYLKL
EECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHH
WDNLDNPAVVEAWLAMNTWVTDNVDFPGAAFRQWVVEFFRENRLMENRLMMEGRVVDVAN
HHCCCCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHH
IRANLLNVIADKDHIVPNCQSRSIMDRVGSKDKKLIELKGGHIGIMVGSGASKRAWPLME
HHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCHHHHH
AWLSERST
HHHHCCCC
>Mature Secondary Structure 
TSSDAAPIDLERMTEQLLKEIERTTKSSDMAARIATNRIRVAVGQTPKEQIWALNKMRL
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEEEECCCCHHHHHHHHHHEE
YHYYPQVPPEQRKPVPILLVFALINRPYIFDLRPGNSFVEYLLQHGYEVYLLDWGRPGLE
EEECCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCC
DAHYDFEDYSLKFLPRAIRAMQRHSGKREFSMLGWCIGATIVTIYAAMRPDDGLRNLILL
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
TAPLDFSDKEGSTFSRWLNTEYYNVDELVRQFGNVPAAVIEYGNKMLKPVENYIGTYLKL
EECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHH
WDNLDNPAVVEAWLAMNTWVTDNVDFPGAAFRQWVVEFFRENRLMENRLMMEGRVVDVAN
HHCCCCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHH
IRANLLNVIADKDHIVPNCQSRSIMDRVGSKDKKLIELKGGHIGIMVGSGASKRAWPLME
HHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCHHHHH
AWLSERST
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1396692 [H]