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The map label for this gene is yciL [C]

Identifier: 222526730

GI number: 222526730

Start: 4363348

End: 4364934

Strand: Direct

Name: yciL [C]

Synonym: Chy400_3504

Alternate gene names: 222526730

Gene position: 4363348-4364934 (Clockwise)

Preceding gene: 222526728

Following gene: 222526731

Centisome position: 82.81

GC content: 62.44

Gene sequence:

>1587_bases
GTGAGCCCTGAACGTCTACAAAAAGTATTGGCCAGCGCTGGTATCGCATCCCGACGTGACTGCGAAGCCCTGATTGCTGC
CGGGCGCGTGATGGTGAATGGTCGGGTCGTTGTTGTACCGGGAACACGTGTTGACCTCGATCACGATGAAGTGTTGGTGG
ATGGTCAACCGTTACAGATGCCGACCAAACGTACCTATATTATGCTGCATAAACCGGCAGGCGTCGTTTCAACCGCAGAA
GATACGCATGGTCGTCCAACAGTGCTCGATCTGGTGAATGTACCGGCACGGGTGTTTCCGGTTGGGCGCCTTGATATGGA
TAGCGAAGGTCTAATTCTCCTCACCGATGACGGCGAGCTGGCCTACGCACTGACACATCCCAGCTTTGAAGTTGAAAAAG
AGTACCGGGTGCTGCTTGATCGAGCACTGACTCCGGAAGCGCTGCGTAGCTGGCGTAACGGGGTTTTGCTCGATGGCGAA
ATGACTGCACCAGCCTGGGTTGAGCTGATTGGCACTGCTCCAGAAGGCGTATGGGTACGGATCGTCCTCCGTGAGGGGCG
GAAGCGCCAGATTCGAGCGGTTGCGAAGCTGCTTGGATATGAGGTGCGTCGATTGATCCGCGTGCGTGAAGGTGATCTGG
TGCTCGGTGATTTGCCACCACGGGCCTGGCGGCATCTGACTGATGAAGAGGTGGCTGCATTACGTACTCACATTGCGAAA
CGTCGCCCCCCGGTAGAGCGATCGCCAGGAGTGGCTCCATCATCGGTACGTAATCGTGATGACGAGCGACCATCCTATCG
CACGCCTGATATTCGATCACGTGGGGATCGTGATGACCGAGGGCGGGAGTTCGAGCGGGAGCCGCGGGCGCGGCGCGATA
GCGGCGAGCGGGGATGGGAGTTTGAGCGAGAACCACGGGCACGGCGCGATAGCGGCGAGCGGGGGCGAGAGTTTGAGCGA
GAACCACGGGCACGGCGCGATAGCGGCGAGCGGGGACGGGAGTTTGAGCGAGAACCACGGGCACGGCGCGATAGCGGCGA
GCGGGGGCGGGAGTTTGAGCGAGAACCACGGGCACGGCGCGATAGCGGCGAGCGGGGGCGGGAGTTTGAGCGAGAACCAC
GGGCACGGCGCGATAGCGGCGAGCGGGGGCGAGAGTTTGAGCGAGAACCACGGGCACGGCGCGATAGCGGCGAGCGGGGG
CGGGAGTTTGAGCGAGAACCACGGGCGCGGCGCGATAGCGGCGAGCGGGGGCGGGAGTTTGAGCGAGAACCACGGGCACG
GCGCGATAGCGGCGAGCGGGGGCGGGAGTTTGAGCGAGAACCACGGGCGCGGCGTGATGCCGGCGAGCGGGGGCGGGAGT
TTGAGCGGGAACCACAGGCGCGGCGTGATGCCGGCGAGCGGGGGCGGGAGTTTGAGCGGGAACCACGGGCACGCAGCGAA
GAACGCTCTCGTTCGACGCCGTCTCGTAGTGGGCCAAAAAGTAAACCACCATCTGCACGATCATCGCAACAGCCGCGCTC
TACCGGAGGGGAACATCAGCGGCGTAACGAACGTGCACGCCGTAACAAAGAGAAAGATCAGGAATAA

Upstream 100 bases:

>100_bases
TGGTATACTGTCAAACGATGATAAACAGTATTTCTTACACAAGGCGAAGACATAAGTCTTCAGGTAGATGGGTGTTGTTT
AAGATACAGAAAGGCTGTCT

Downstream 100 bases:

>100_bases
CTTCAATGCTTACAAACTCTGAAAGAGCGGTGCTGATCAGCTTGACAATGGTTAAGATAGTGGTATAAATGTTGACGTGT
ATACTTGTGATAGATGCCAC

Product: pseudouridine synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSPERLQKVLASAGIASRRDCEALIAAGRVMVNGRVVVVPGTRVDLDHDEVLVDGQPLQMPTKRTYIMLHKPAGVVSTAE
DTHGRPTVLDLVNVPARVFPVGRLDMDSEGLILLTDDGELAYALTHPSFEVEKEYRVLLDRALTPEALRSWRNGVLLDGE
MTAPAWVELIGTAPEGVWVRIVLREGRKRQIRAVAKLLGYEVRRLIRVREGDLVLGDLPPRAWRHLTDEEVAALRTHIAK
RRPPVERSPGVAPSSVRNRDDERPSYRTPDIRSRGDRDDRGREFEREPRARRDSGERGWEFEREPRARRDSGERGREFER
EPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERG
REFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDAGERGREFEREPQARRDAGERGREFEREPRARSE
ERSRSTPSRSGPKSKPPSARSSQQPRSTGGEHQRRNERARRNKEKDQE

Sequences:

>Translated_528_residues
MSPERLQKVLASAGIASRRDCEALIAAGRVMVNGRVVVVPGTRVDLDHDEVLVDGQPLQMPTKRTYIMLHKPAGVVSTAE
DTHGRPTVLDLVNVPARVFPVGRLDMDSEGLILLTDDGELAYALTHPSFEVEKEYRVLLDRALTPEALRSWRNGVLLDGE
MTAPAWVELIGTAPEGVWVRIVLREGRKRQIRAVAKLLGYEVRRLIRVREGDLVLGDLPPRAWRHLTDEEVAALRTHIAK
RRPPVERSPGVAPSSVRNRDDERPSYRTPDIRSRGDRDDRGREFEREPRARRDSGERGWEFEREPRARRDSGERGREFER
EPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERG
REFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDAGERGREFEREPQARRDAGERGREFEREPRARSE
ERSRSTPSRSGPKSKPPSARSSQQPRSTGGEHQRRNERARRNKEKDQE
>Mature_527_residues
SPERLQKVLASAGIASRRDCEALIAAGRVMVNGRVVVVPGTRVDLDHDEVLVDGQPLQMPTKRTYIMLHKPAGVVSTAED
THGRPTVLDLVNVPARVFPVGRLDMDSEGLILLTDDGELAYALTHPSFEVEKEYRVLLDRALTPEALRSWRNGVLLDGEM
TAPAWVELIGTAPEGVWVRIVLREGRKRQIRAVAKLLGYEVRRLIRVREGDLVLGDLPPRAWRHLTDEEVAALRTHIAKR
RPPVERSPGVAPSSVRNRDDERPSYRTPDIRSRGDRDDRGREFEREPRARRDSGERGWEFEREPRARRDSGERGREFERE
PRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGR
EFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDAGERGREFEREPQARRDAGERGREFEREPRARSEE
RSRSTPSRSGPKSKPPSARSSQQPRSTGGEHQRRNERARRNKEKDQE

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=39.344262295082, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790453, Length=244, Percent_Identity=38.1147540983607, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=34.8936170212766, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=36.5168539325843, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 61343; Mature: 61212

Theoretical pI: Translated: 11.14; Mature: 11.14

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPERLQKVLASAGIASRRDCEALIAAGRVMVNGRVVVVPGTRVDLDHDEVLVDGQPLQM
CCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCEEEEECCCEECCCCCCEEECCCCCCC
PTKRTYIMLHKPAGVVSTAEDTHGRPTVLDLVNVPARVFPVGRLDMDSEGLILLTDDGEL
CCCCEEEEEECCCCCEECCCCCCCCCHHHHHHCCCHHHEECCCCCCCCCCEEEEECCCCE
AYALTHPSFEVEKEYRVLLDRALTPEALRSWRNGVLLDGEMTAPAWVELIGTAPEGVWVR
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCCCCHHHHHHHCCCCCCEEEE
IVLREGRKRQIRAVAKLLGYEVRRLIRVREGDLVLGDLPPRAWRHLTDEEVAALRTHIAK
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHH
RRPPVERSPGVAPSSVRNRDDERPSYRTPDIRSRGDRDDRGREFEREPRARRDSGERGWE
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHCCCCCCCCC
FEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARR
CCCCCCHHCCCCCCCCHHHHCHHHHCCCCCCCCHHHHCHHHHCCCCCCCCHHHHCHHHHC
DSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREF
CCCCCCCHHHHCHHHHCCCCCCCCHHHHCHHHHCCCCCCCCHHHHCHHHHCCCCCCCCHH
EREPRARRDSGERGREFEREPRARRDAGERGREFEREPQARRDAGERGREFEREPRARSE
HHCHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHH
ERSRSTPSRSGPKSKPPSARSSQQPRSTGGEHQRRNERARRNKEKDQE
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SPERLQKVLASAGIASRRDCEALIAAGRVMVNGRVVVVPGTRVDLDHDEVLVDGQPLQM
CHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCEEEEECCCEECCCCCCEEECCCCCCC
PTKRTYIMLHKPAGVVSTAEDTHGRPTVLDLVNVPARVFPVGRLDMDSEGLILLTDDGEL
CCCCEEEEEECCCCCEECCCCCCCCCHHHHHHCCCHHHEECCCCCCCCCCEEEEECCCCE
AYALTHPSFEVEKEYRVLLDRALTPEALRSWRNGVLLDGEMTAPAWVELIGTAPEGVWVR
EEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCCCCHHHHHHHCCCCCCEEEE
IVLREGRKRQIRAVAKLLGYEVRRLIRVREGDLVLGDLPPRAWRHLTDEEVAALRTHIAK
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHH
RRPPVERSPGVAPSSVRNRDDERPSYRTPDIRSRGDRDDRGREFEREPRARRDSGERGWE
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCHHHHCCCCCCCCC
FEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARR
CCCCCCHHCCCCCCCCHHHHCHHHHCCCCCCCCHHHHCHHHHCCCCCCCCHHHHCHHHHC
DSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREFEREPRARRDSGERGREF
CCCCCCCHHHHCHHHHCCCCCCCCHHHHCHHHHCCCCCCCCHHHHCHHHHCCCCCCCCHH
EREPRARRDSGERGREFEREPRARRDAGERGREFEREPQARRDAGERGREFEREPRARSE
HHCHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHH
ERSRSTPSRSGPKSKPPSARSSQQPRSTGGEHQRRNERARRNKEKDQE
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]