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The map label for this gene is cysL [H]

Identifier: 222526689

GI number: 222526689

Start: 4315277

End: 4316158

Strand: Direct

Name: cysL [H]

Synonym: Chy400_3459

Alternate gene names: 222526689

Gene position: 4315277-4316158 (Clockwise)

Preceding gene: 222526685

Following gene: 222526691

Centisome position: 81.9

GC content: 54.31

Gene sequence:

>882_bases
GTGACACTTAATCTGCATTTACTACGCATTTACGTTGCCGTGCTTGAACAGGGCAGTTTCACGAAAGCAGCCGAAGTCCT
CACAATGAGTCAATCGGCGGTATCGCGGGCAGTGCAAGAGCTTGAACGTCAACTTGGCACGACTCTGCTCGAACGGCGTG
CGCGCGGTGTGGCCCCAACGATGGCCGGTACTATCCTGGGTGAGCATGCCCGACGCATCTTTACGCATGAGCGGCTGGCT
ATCGAGGCGTTGAACGAACTACGCGGTCTGCAACGAGGTCGGCTGGCAATTGGTGCAAGTAGTACGATTGGTATTTACCT
GCTACCCCCACTCCTCGGTAACTATCATCGTCGGTATCCGGGCATCGAGTTGTTCCTGGATATTGGCAATACCCAACAGA
TTATTGAACGCCTGTTATCCTATCAGATTGATGTCGCTTACGTGGAAGGGCCGGTAGCAGCGAACGAAGACTTGCAGGTA
GTGCCGTGGCGAAGTGACGAGATGGTTGTGATTGCCTCTCCCGATCATCCGCTTACCCGTCGGGTCACCCTGGATTGTTC
TGATCTCCAGGATGCCCCTTTCATTATGCGTGAACCTGGCTCAGGCACACGTAAGGTGATGGAACAGGCACTGGCAGCAC
ATAACGTGACGGTACGTCCGGTGATGGAATTGGGGAGTACCGAAGCCGTGAAGCAGGCAGTGAGTGCCGGATTAGGGTTG
AGCATGGTTTCGCGCTTTACGATCCAATCTGAGCTGATTGCCGGTAAGCTGCGGGTACTCAATATTCCTGGTCTCAATAT
ACAACGTCAGTTGAGCCGCGTCCGTCTGCACGACCGTCCTCAGAGTCTGGCCCTGGAAGCATGGTTTAAGAATCGTGGCT
GA

Upstream 100 bases:

>100_bases
TGCCTCATTGCATAGAGTATGTCAGTGGGAAGTGACGTATTCGCAGAAAGGGCCAGCTATGGTATATATTGCATCAGAAA
TAAAATTGTTGGGAGGCAAA

Downstream 100 bases:

>100_bases
GGTATTGGCGTCCGCGCTCGATATCACACGTCGGTTGTACACCTGCCTCGGTAGCAAGGTGTGGTTTTTGTATCAGAGAG
CTGACTGGCGGGTACGGGAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTLNLHLLRIYVAVLEQGSFTKAAEVLTMSQSAVSRAVQELERQLGTTLLERRARGVAPTMAGTILGEHARRIFTHERLA
IEALNELRGLQRGRLAIGASSTIGIYLLPPLLGNYHRRYPGIELFLDIGNTQQIIERLLSYQIDVAYVEGPVAANEDLQV
VPWRSDEMVVIASPDHPLTRRVTLDCSDLQDAPFIMREPGSGTRKVMEQALAAHNVTVRPVMELGSTEAVKQAVSAGLGL
SMVSRFTIQSELIAGKLRVLNIPGLNIQRQLSRVRLHDRPQSLALEAWFKNRG

Sequences:

>Translated_293_residues
MTLNLHLLRIYVAVLEQGSFTKAAEVLTMSQSAVSRAVQELERQLGTTLLERRARGVAPTMAGTILGEHARRIFTHERLA
IEALNELRGLQRGRLAIGASSTIGIYLLPPLLGNYHRRYPGIELFLDIGNTQQIIERLLSYQIDVAYVEGPVAANEDLQV
VPWRSDEMVVIASPDHPLTRRVTLDCSDLQDAPFIMREPGSGTRKVMEQALAAHNVTVRPVMELGSTEAVKQAVSAGLGL
SMVSRFTIQSELIAGKLRVLNIPGLNIQRQLSRVRLHDRPQSLALEAWFKNRG
>Mature_292_residues
TLNLHLLRIYVAVLEQGSFTKAAEVLTMSQSAVSRAVQELERQLGTTLLERRARGVAPTMAGTILGEHARRIFTHERLAI
EALNELRGLQRGRLAIGASSTIGIYLLPPLLGNYHRRYPGIELFLDIGNTQQIIERLLSYQIDVAYVEGPVAANEDLQVV
PWRSDEMVVIASPDHPLTRRVTLDCSDLQDAPFIMREPGSGTRKVMEQALAAHNVTVRPVMELGSTEAVKQAVSAGLGLS
MVSRFTIQSELIAGKLRVLNIPGLNIQRQLSRVRLHDRPQSLALEAWFKNRG

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=288, Percent_Identity=37.8472222222222, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788748, Length=291, Percent_Identity=29.8969072164948, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI157672245, Length=223, Percent_Identity=28.6995515695067, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790399, Length=253, Percent_Identity=28.4584980237154, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=31.1188811188811, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789204, Length=289, Percent_Identity=28.3737024221453, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI145693105, Length=291, Percent_Identity=25.7731958762887, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788297, Length=244, Percent_Identity=27.8688524590164, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787601, Length=178, Percent_Identity=29.2134831460674, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082132, Length=251, Percent_Identity=29.8804780876494, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787879, Length=246, Percent_Identity=29.2682926829268, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787530, Length=253, Percent_Identity=24.5059288537549, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788296, Length=248, Percent_Identity=27.4193548387097, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI2367136, Length=264, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081904, Length=210, Percent_Identity=27.6190476190476, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787806, Length=250, Percent_Identity=23.6, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788887, Length=249, Percent_Identity=28.5140562248996, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790208, Length=258, Percent_Identity=25.1937984496124, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI145693193, Length=266, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789440, Length=125, Percent_Identity=32, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789639, Length=125, Percent_Identity=35.2, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=278, Percent_Identity=29.136690647482, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=260, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788626, Length=263, Percent_Identity=26.9961977186312, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787128, Length=131, Percent_Identity=30.5343511450382, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786448, Length=172, Percent_Identity=30.2325581395349, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32483; Mature: 32352

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNLHLLRIYVAVLEQGSFTKAAEVLTMSQSAVSRAVQELERQLGTTLLERRARGVAPT
CEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MAGTILGEHARRIFTHERLAIEALNELRGLQRGRLAIGASSTIGIYLLPPLLGNYHRRYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEECCHHHCCHHHHCC
GIELFLDIGNTQQIIERLLSYQIDVAYVEGPVAANEDLQVVPWRSDEMVVIASPDHPLTR
CEEEEEECCCHHHHHHHHHHCEEEEEEEECCCCCCCCCEEEECCCCCEEEEECCCCCCEE
RVTLDCSDLQDAPFIMREPGSGTRKVMEQALAAHNVTVRPVMELGSTEAVKQAVSAGLGL
EEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEHHHHHCCCHHHHHHHHHHCCCH
SMVSRFTIQSELIAGKLRVLNIPGLNIQRQLSRVRLHDRPQSLALEAWFKNRG
HHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TLNLHLLRIYVAVLEQGSFTKAAEVLTMSQSAVSRAVQELERQLGTTLLERRARGVAPT
EEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MAGTILGEHARRIFTHERLAIEALNELRGLQRGRLAIGASSTIGIYLLPPLLGNYHRRYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEECCHHHCCHHHHCC
GIELFLDIGNTQQIIERLLSYQIDVAYVEGPVAANEDLQVVPWRSDEMVVIASPDHPLTR
CEEEEEECCCHHHHHHHHHHCEEEEEEEECCCCCCCCCEEEECCCCCEEEEECCCCCCEE
RVTLDCSDLQDAPFIMREPGSGTRKVMEQALAAHNVTVRPVMELGSTEAVKQAVSAGLGL
EEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEHHHHHCCCHHHHHHHHHHCCCH
SMVSRFTIQSELIAGKLRVLNIPGLNIQRQLSRVRLHDRPQSLALEAWFKNRG
HHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]