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The map label for this gene is nfrA1 [H]

Identifier: 222526652

GI number: 222526652

Start: 4262612

End: 4263442

Strand: Reverse

Name: nfrA1 [H]

Synonym: Chy400_3421

Alternate gene names: 222526652

Gene position: 4263442-4262612 (Counterclockwise)

Preceding gene: 222526653

Following gene: 222526651

Centisome position: 80.92

GC content: 57.88

Gene sequence:

>831_bases
ATGACCGATACCACAACCACAACGCAAGAACTCTTACACCAGCGTTACGGCAATGATATTCCGCCATCCATCATCAGCAC
CCCGATCATCGACCTGTTACTGAGTCACCGTTCTGTACGTTCGTACAGTAACGAACCACTGCCGGAAGGCACGCTTGAAA
CGATGATTGCAGCGGCCCAATCTGCGGCCACATCATCAAATCTCCAGACGTGGAGTGTGGTTGCGGTGACTGACCCCGAA
CGAAAGCATCGCCTGGCGACCCTGGCCGGTGATCAAAACCAGATTCGGCGGGCACCGCTTTTCCTGGTCTGGCTGGCCGA
TCTGCACCGCATTGCCCAGGCGGCTGATGCCCGGCAGATGCCACACGAGGGGCTTGACTACCTGGAAATGTGGCTGGTGG
GTGTGATAGATGCGGCCCTGGCAGCACAGAATGCTGTCGTTGCCGCCGAGTCGCTCGGCCTCGGTACCGTCTACATTGGC
GCCATTCGCAATCATCCACTGGAGGTAGCGGCTGAATTGCAACTGCCACCGATGGTGATGCCGGTGTTCGGGATGTGTGT
GGGTCGTCCCAATCCGGCACGACCGGCAGCGATTAAACCACGTTTGCCCCAGGAGGCGGTACTTCACCGGGAAGTCTACC
GGCCAGAGCAGATACCACCTGCTGTCGAACGTTACAACGCGACAATGAGCGCCTTTTACGCGGCCCAACAGATGAATGTA
GAAGGCGATTGGGTCGATCACTCGGCACGTCGGGTTGCCGGGCCGCATACCCTTTCGGGTCGTGATGTCCTGCGTGAAGT
GCTGCATCGACTCGGTTTTCGGTTGAAATAA

Upstream 100 bases:

>100_bases
CCGTTGCAGTGTCTATCCTGTTACCGGATTGTCGCGCAGTTTCGTGTATAATCGGCATATCTTTGACTTAACAACCAATC
ATGTAGAGGAACCATTATCT

Downstream 100 bases:

>100_bases
GACAACTTTACGCATAAGTACGCGCTTCTCCCGCTGTATCGTCTTTGGTGCAGCGGGAGATATGTTATACTTTGAATAGG
TTTAAACAGATTTAACAAGA

Product: nitroreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTDTTTTTQELLHQRYGNDIPPSIISTPIIDLLLSHRSVRSYSNEPLPEGTLETMIAAAQSAATSSNLQTWSVVAVTDPE
RKHRLATLAGDQNQIRRAPLFLVWLADLHRIAQAADARQMPHEGLDYLEMWLVGVIDAALAAQNAVVAAESLGLGTVYIG
AIRNHPLEVAAELQLPPMVMPVFGMCVGRPNPARPAAIKPRLPQEAVLHREVYRPEQIPPAVERYNATMSAFYAAQQMNV
EGDWVDHSARRVAGPHTLSGRDVLREVLHRLGFRLK

Sequences:

>Translated_276_residues
MTDTTTTTQELLHQRYGNDIPPSIISTPIIDLLLSHRSVRSYSNEPLPEGTLETMIAAAQSAATSSNLQTWSVVAVTDPE
RKHRLATLAGDQNQIRRAPLFLVWLADLHRIAQAADARQMPHEGLDYLEMWLVGVIDAALAAQNAVVAAESLGLGTVYIG
AIRNHPLEVAAELQLPPMVMPVFGMCVGRPNPARPAAIKPRLPQEAVLHREVYRPEQIPPAVERYNATMSAFYAAQQMNV
EGDWVDHSARRVAGPHTLSGRDVLREVLHRLGFRLK
>Mature_275_residues
TDTTTTTQELLHQRYGNDIPPSIISTPIIDLLLSHRSVRSYSNEPLPEGTLETMIAAAQSAATSSNLQTWSVVAVTDPER
KHRLATLAGDQNQIRRAPLFLVWLADLHRIAQAADARQMPHEGLDYLEMWLVGVIDAALAAQNAVVAAESLGLGTVYIGA
IRNHPLEVAAELQLPPMVMPVFGMCVGRPNPARPAAIKPRLPQEAVLHREVYRPEQIPPAVERYNATMSAFYAAQQMNVE
GDWVDHSARRVAGPHTLSGRDVLREVLHRLGFRLK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=248, Percent_Identity=37.0967741935484, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 30447; Mature: 30316

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTTTTTQELLHQRYGNDIPPSIISTPIIDLLLSHRSVRSYSNEPLPEGTLETMIAAAQ
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
SAATSSNLQTWSVVAVTDPERKHRLATLAGDQNQIRRAPLFLVWLADLHRIAQAADARQM
HHHCCCCCCEEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PHEGLDYLEMWLVGVIDAALAAQNAVVAAESLGLGTVYIGAIRNHPLEVAAELQLPPMVM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCHHHHCCCCCCCHHH
PVFGMCVGRPNPARPAAIKPRLPQEAVLHREVYRPEQIPPAVERYNATMSAFYAAQQMNV
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EGDWVDHSARRVAGPHTLSGRDVLREVLHRLGFRLK
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDTTTTTQELLHQRYGNDIPPSIISTPIIDLLLSHRSVRSYSNEPLPEGTLETMIAAAQ
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
SAATSSNLQTWSVVAVTDPERKHRLATLAGDQNQIRRAPLFLVWLADLHRIAQAADARQM
HHHCCCCCCEEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PHEGLDYLEMWLVGVIDAALAAQNAVVAAESLGLGTVYIGAIRNHPLEVAAELQLPPMVM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCHHHHCCCCCCCHHH
PVFGMCVGRPNPARPAAIKPRLPQEAVLHREVYRPEQIPPAVERYNATMSAFYAAQQMNV
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EGDWVDHSARRVAGPHTLSGRDVLREVLHRLGFRLK
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]