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The map label for this gene is malE [H]

Identifier: 222526636

GI number: 222526636

Start: 4240914

End: 4242227

Strand: Direct

Name: malE [H]

Synonym: Chy400_3404

Alternate gene names: 222526636

Gene position: 4240914-4242227 (Clockwise)

Preceding gene: 222526634

Following gene: 222526637

Centisome position: 80.49

GC content: 59.82

Gene sequence:

>1314_bases
GTGAGTGCAATGAAGCGTGCTTTGATTGCTCTGTTCCTCGCCGCCGCAATGATTTTGGCGGCATGTGGTTCGAATCAGAC
GGCCACTCAGCCGACCACCGCTCCTGCCGAGCAGCCGACCACCGCTCCTGCCGAGCAGCCGACCACCGCTCCTGCCGAGC
AGCCCACCACCGCACCCTCTGCTCAACAGGTTACCATTCGTATCTGGCATCAGTGGGATGGTGCGTATCTGACTGCGATT
GAGCAGGCTTTCCGTGATTACGAAGCCGCCAATCCGAATGTCAAGATCGATCTCTCGAAGCCGGAAGATGTCAGCAATGC
GTTGAATGTGGCGATTCCCGCCGGTGAAGGCCCCGATATTATCGGTTGGGCGAATGACCAGATCGGTCAGCAGGCACTGG
TTGGCAACATCGTCGCGCTGAACGATTACGGCATCACCGAGGATTTCCTGCGCAGCACCTATGAGCCGGCTGCAGTCAAT
GGTGTGATCTGGCAGGGCAAGATTTGGGCATTGCCGGAGACCCAGGAAGGTATTGCTCTAATCTACAACAAGGCAGTCGT
GGGCGATATGCAGTTGCCGACCAGCCTCGATGAGCTGTTGCAGATGGCGAAGGATTTCCGCGCTGCCAACCCTGACAAGA
CCTTCGTCTGCAACCAGGGCTTCGGTGGTAACGATGCGTACCACGTCGCTCCGATCTACTTCGGCTACGGTGTTCCGAGC
TATGTTGACGATGAGGGCAATGTCTACGTTAACACGCCGGAGATGATCAAGGGTGGCGAGTGGCTGGCCGAGATGAGCAA
GGTCTCGTTCAGCGAGCAGAGCTACGACATCTGCAAGGCTGCTCTGGCTGAAGGCAAGGCGGCTATGTGGTGGACCGGCC
CGTGGGCGATTGCCGGTATCGAGCAGGATGGTGTTGATTACGGCATTCTGCCGATGGGCAAGCCCTTCGTCGGTATCAAG
ACCCTGATGCTGACCCGCAATGCGGTTGAGCGCGGCAATGCTGAGGTGGCGCTCGATATTATGAAGTACTTCACCAGCGC
CGAGGTGCAGGCGAAGCTGGCCTTGACCAACAAGACGGTGCCCGCAGCGACCGCAGCGCTCCAGAACCCCGAGGTCGCCG
CGCTGCCCACGCTGGCCGGCTTTGGTGCCGCCCTTAATACGGGTGTGCCGATGGCCAACACCCCCTTCGCTTCGGCCCAG
TGGGGTCCGGTCGGTGAGGCAAGCGTTGCCATCTGGACCGGTGCGCAGACGCCGGCTGAGGCGCTGGCCGCTGCCCAGCA
GGCGATTGAAGCTGCGATTATGCAGATGAAGTAA

Upstream 100 bases:

>100_bases
CGGGTCTAATGCCTTTTCGATGAATTTGTCTATCTGTTCGTCATTCCAGATTTCCGTTGTTGTGCTGTTGTCGTAGAAGA
CACAGTCAAGGAGTACAACC

Downstream 100 bases:

>100_bases
ACCTTCCGTGTAGGGGTGAGCAAGGTTCGCTCACCCCTTTTGAACGCCGTCGTTATCAACAACACGAGCCGGACCACGCT
GTCCGGCTCACTCACTACAC

Product: family 1 extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSAMKRALIALFLAAAMILAACGSNQTATQPTTAPAEQPTTAPAEQPTTAPAEQPTTAPSAQQVTIRIWHQWDGAYLTAI
EQAFRDYEAANPNVKIDLSKPEDVSNALNVAIPAGEGPDIIGWANDQIGQQALVGNIVALNDYGITEDFLRSTYEPAAVN
GVIWQGKIWALPETQEGIALIYNKAVVGDMQLPTSLDELLQMAKDFRAANPDKTFVCNQGFGGNDAYHVAPIYFGYGVPS
YVDDEGNVYVNTPEMIKGGEWLAEMSKVSFSEQSYDICKAALAEGKAAMWWTGPWAIAGIEQDGVDYGILPMGKPFVGIK
TLMLTRNAVERGNAEVALDIMKYFTSAEVQAKLALTNKTVPAATAALQNPEVAALPTLAGFGAALNTGVPMANTPFASAQ
WGPVGEASVAIWTGAQTPAEALAAAQQAIEAAIMQMK

Sequences:

>Translated_437_residues
MSAMKRALIALFLAAAMILAACGSNQTATQPTTAPAEQPTTAPAEQPTTAPAEQPTTAPSAQQVTIRIWHQWDGAYLTAI
EQAFRDYEAANPNVKIDLSKPEDVSNALNVAIPAGEGPDIIGWANDQIGQQALVGNIVALNDYGITEDFLRSTYEPAAVN
GVIWQGKIWALPETQEGIALIYNKAVVGDMQLPTSLDELLQMAKDFRAANPDKTFVCNQGFGGNDAYHVAPIYFGYGVPS
YVDDEGNVYVNTPEMIKGGEWLAEMSKVSFSEQSYDICKAALAEGKAAMWWTGPWAIAGIEQDGVDYGILPMGKPFVGIK
TLMLTRNAVERGNAEVALDIMKYFTSAEVQAKLALTNKTVPAATAALQNPEVAALPTLAGFGAALNTGVPMANTPFASAQ
WGPVGEASVAIWTGAQTPAEALAAAQQAIEAAIMQMK
>Mature_436_residues
SAMKRALIALFLAAAMILAACGSNQTATQPTTAPAEQPTTAPAEQPTTAPAEQPTTAPSAQQVTIRIWHQWDGAYLTAIE
QAFRDYEAANPNVKIDLSKPEDVSNALNVAIPAGEGPDIIGWANDQIGQQALVGNIVALNDYGITEDFLRSTYEPAAVNG
VIWQGKIWALPETQEGIALIYNKAVVGDMQLPTSLDELLQMAKDFRAANPDKTFVCNQGFGGNDAYHVAPIYFGYGVPSY
VDDEGNVYVNTPEMIKGGEWLAEMSKVSFSEQSYDICKAALAEGKAAMWWTGPWAIAGIEQDGVDYGILPMGKPFVGIKT
LMLTRNAVERGNAEVALDIMKYFTSAEVQAKLALTNKTVPAATAALQNPEVAALPTLAGFGAALNTGVPMANTPFASAQW
GPVGEASVAIWTGAQTPAEALAAAQQAIEAAIMQMK

Specific function: Involved in an abc transport system for maltotriose [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=387, Percent_Identity=27.6485788113695, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 46358; Mature: 46227

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAMKRALIALFLAAAMILAACGSNQTATQPTTAPAEQPTTAPAEQPTTAPAEQPTTAPS
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQQVTIRIWHQWDGAYLTAIEQAFRDYEAANPNVKIDLSKPEDVSNALNVAIPAGEGPDI
CCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCCCE
IGWANDQIGQQALVGNIVALNDYGITEDFLRSTYEPAAVNGVIWQGKIWALPETQEGIAL
EEECCCHHCHHHHHCCEEEEECCCCCHHHHHCCCCCCCCCCEEECCEEEECCCCCCCEEE
IYNKAVVGDMQLPTSLDELLQMAKDFRAANPDKTFVCNQGFGGNDAYHVAPIYFGYGVPS
EEECEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCC
YVDDEGNVYVNTPEMIKGGEWLAEMSKVSFSEQSYDICKAALAEGKAAMWWTGPWAIAGI
EECCCCCEEECCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEC
EQDGVDYGILPMGKPFVGIKTLMLTRNAVERGNAEVALDIMKYFTSAEVQAKLALTNKTV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCC
PAATAALQNPEVAALPTLAGFGAALNTGVPMANTPFASAQWGPVGEASVAIWTGAQTPAE
CHHHHHHCCCCCEECCCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
ALAAAQQAIEAAIMQMK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAMKRALIALFLAAAMILAACGSNQTATQPTTAPAEQPTTAPAEQPTTAPAEQPTTAPS
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQQVTIRIWHQWDGAYLTAIEQAFRDYEAANPNVKIDLSKPEDVSNALNVAIPAGEGPDI
CCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEECCCCCCE
IGWANDQIGQQALVGNIVALNDYGITEDFLRSTYEPAAVNGVIWQGKIWALPETQEGIAL
EEECCCHHCHHHHHCCEEEEECCCCCHHHHHCCCCCCCCCCEEECCEEEECCCCCCCEEE
IYNKAVVGDMQLPTSLDELLQMAKDFRAANPDKTFVCNQGFGGNDAYHVAPIYFGYGVPS
EEECEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCC
YVDDEGNVYVNTPEMIKGGEWLAEMSKVSFSEQSYDICKAALAEGKAAMWWTGPWAIAGI
EECCCCCEEECCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEC
EQDGVDYGILPMGKPFVGIKTLMLTRNAVERGNAEVALDIMKYFTSAEVQAKLALTNKTV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCC
PAATAALQNPEVAALPTLAGFGAALNTGVPMANTPFASAQWGPVGEASVAIWTGAQTPAE
CHHHHHHCCCCCEECCCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
ALAAAQQAIEAAIMQMK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]