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The map label for this gene is acoA [H]

Identifier: 222526601

GI number: 222526601

Start: 4198224

End: 4199189

Strand: Direct

Name: acoA [H]

Synonym: Chy400_3369

Alternate gene names: 222526601

Gene position: 4198224-4199189 (Clockwise)

Preceding gene: 222526600

Following gene: 222526602

Centisome position: 79.68

GC content: 55.8

Gene sequence:

>966_bases
ATGGACGAGATTAATGATGAGTTATTGCAACAGGCCCATTATTGGATGCGCCTTACCCGTGCGCTCGATGACCGGGGTAC
GTTTCTGCACAAGCAGAGCAAAATTGTCGGTGGCTACTTTTCGCAAATCGGGCACGAGGCGCTCAGTGTTGCAGCGGCGC
TAAGCCTGGGGCCACGCGATATTATTGCGCCGATGCACCGCGATTTGGGGGCCTACCTGGTGCGTGGCTTGACCCCGAAG
CGTATTCTTGCTCAGTGGTTAGGACGCGAGACCGGTGTGACCCGTGGACGTGACGCGAATCTACACGGTATGGGTGATCT
AAGCCTGGGCATTATTGGCTTTATCAGCCATCTGCCGGCTTCGACCGGCGTGATCACCGGTGTTGCTCACGCAATCAAGC
TCAAAGGCGAACCACGGGTTGCCATGTGTTTTTTTGGTGATGGCAGTGCTTCACAAGGACTGGCTCATGAAGCGATGAAT
TGGGCGAGTGTCTTTAAATTGCCGATGGTGATTATCTGCGAAAATAATCAGTACGCCTACTCAACCCCGCTGAGCCGGCA
GATGGCAATCACAGATATTGCCCAGCGGGCAGCCGGTTACGCGATGCCGGGGGTGATTGTAGATGGCAACGATTTTGCCG
CAGTGTATCGTGCAACCAAAGAAGCGGTCGAACGGGCACGCGCTGGTGGTGGGCCGACATTCATCGAATGTAAGACGATG
CGTATGCGCGGTCACGCCATTCACGACAATATGGCCTACGTGCCGAAAGAATTGCTCGCCGAATGGGAAGCTCGCGACCC
AATTGCCCGTATAGAGGAAGTGTTGCGCAGCCGTGGTTTACTCGATGATGCAAAGCTGGCTGCCCTGTTGGCCCGGATCG
AAGCCGAGCTTGATGAGGCACAGGCGTTCGCTGAAGCTTCACCGTATCCCGATCCGGCAACGCTGACCGATGGCGTCTAT
GCGTGA

Upstream 100 bases:

>100_bases
CGGCTACGTAAATCGAGTACAATAGAAACATATCCCTTTGAATGGCGAAAAACGGCAATGGACGCCGGGCAAGCGGTGCC
TTCACCGGCAAGGAGATGGC

Downstream 100 bases:

>100_bases
ACCACGAGCAGGAGGCAGGCAATGACGTGGGATCAAGGTTTACATAAATCGACAACCGTCCTGAGTGACGATCAGGGGAC
ACGCGAGTTGACCTATCTGG

Product: pyruvate dehydrogenase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDEINDELLQQAHYWMRLTRALDDRGTFLHKQSKIVGGYFSQIGHEALSVAAALSLGPRDIIAPMHRDLGAYLVRGLTPK
RILAQWLGRETGVTRGRDANLHGMGDLSLGIIGFISHLPASTGVITGVAHAIKLKGEPRVAMCFFGDGSASQGLAHEAMN
WASVFKLPMVIICENNQYAYSTPLSRQMAITDIAQRAAGYAMPGVIVDGNDFAAVYRATKEAVERARAGGGPTFIECKTM
RMRGHAIHDNMAYVPKELLAEWEARDPIARIEEVLRSRGLLDDAKLAALLARIEAELDEAQAFAEASPYPDPATLTDGVY
A

Sequences:

>Translated_321_residues
MDEINDELLQQAHYWMRLTRALDDRGTFLHKQSKIVGGYFSQIGHEALSVAAALSLGPRDIIAPMHRDLGAYLVRGLTPK
RILAQWLGRETGVTRGRDANLHGMGDLSLGIIGFISHLPASTGVITGVAHAIKLKGEPRVAMCFFGDGSASQGLAHEAMN
WASVFKLPMVIICENNQYAYSTPLSRQMAITDIAQRAAGYAMPGVIVDGNDFAAVYRATKEAVERARAGGGPTFIECKTM
RMRGHAIHDNMAYVPKELLAEWEARDPIARIEEVLRSRGLLDDAKLAALLARIEAELDEAQAFAEASPYPDPATLTDGVY
A
>Mature_321_residues
MDEINDELLQQAHYWMRLTRALDDRGTFLHKQSKIVGGYFSQIGHEALSVAAALSLGPRDIIAPMHRDLGAYLVRGLTPK
RILAQWLGRETGVTRGRDANLHGMGDLSLGIIGFISHLPASTGVITGVAHAIKLKGEPRVAMCFFGDGSASQGLAHEAMN
WASVFKLPMVIICENNQYAYSTPLSRQMAITDIAQRAAGYAMPGVIVDGNDFAAVYRATKEAVERARAGGGPTFIECKTM
RMRGHAIHDNMAYVPKELLAEWEARDPIARIEEVLRSRGLLDDAKLAALLARIEAELDEAQAFAEASPYPDPATLTDGVY
A

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=312, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI11386135, Length=299, Percent_Identity=33.1103678929766, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI258645172, Length=299, Percent_Identity=33.1103678929766, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI4505685, Length=308, Percent_Identity=31.8181818181818, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI291084742, Length=308, Percent_Identity=31.8181818181818, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI291084744, Length=316, Percent_Identity=31.0126582278481, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI291084757, Length=308, Percent_Identity=27.9220779220779, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI86563355, Length=265, Percent_Identity=33.9622641509434, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI86563357, Length=265, Percent_Identity=33.9622641509434, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32564172, Length=307, Percent_Identity=30.6188925081433, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17536047, Length=307, Percent_Identity=30.6188925081433, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321026, Length=311, Percent_Identity=30.5466237942122, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24639746, Length=308, Percent_Identity=31.8181818181818, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24639744, Length=308, Percent_Identity=31.8181818181818, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI28571106, Length=308, Percent_Identity=31.8181818181818, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24639740, Length=308, Percent_Identity=31.8181818181818, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21355903, Length=329, Percent_Identity=28.8753799392097, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24639748, Length=272, Percent_Identity=31.25, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 34958; Mature: 34958

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEINDELLQQAHYWMRLTRALDDRGTFLHKQSKIVGGYFSQIGHEALSVAAALSLGPRD
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IIAPMHRDLGAYLVRGLTPKRILAQWLGRETGVTRGRDANLHGMGDLSLGIIGFISHLPA
HHHHHHHHHHHHHHHCCCHHHHHHHHHCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
STGVITGVAHAIKLKGEPRVAMCFFGDGSASQGLAHEAMNWASVFKLPMVIICENNQYAY
CCHHHHHHHHHHEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEE
STPLSRQMAITDIAQRAAGYAMPGVIVDGNDFAAVYRATKEAVERARAGGGPTFIECKTM
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEH
RMRGHAIHDNMAYVPKELLAEWEARDPIARIEEVLRSRGLLDDAKLAALLARIEAELDEA
HHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QAFAEASPYPDPATLTDGVYA
HHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDEINDELLQQAHYWMRLTRALDDRGTFLHKQSKIVGGYFSQIGHEALSVAAALSLGPRD
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IIAPMHRDLGAYLVRGLTPKRILAQWLGRETGVTRGRDANLHGMGDLSLGIIGFISHLPA
HHHHHHHHHHHHHHHCCCHHHHHHHHHCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
STGVITGVAHAIKLKGEPRVAMCFFGDGSASQGLAHEAMNWASVFKLPMVIICENNQYAY
CCHHHHHHHHHHEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEE
STPLSRQMAITDIAQRAAGYAMPGVIVDGNDFAAVYRATKEAVERARAGGGPTFIECKTM
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEH
RMRGHAIHDNMAYVPKELLAEWEARDPIARIEEVLRSRGLLDDAKLAALLARIEAELDEA
HHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
QAFAEASPYPDPATLTDGVYA
HHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]