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The map label for this gene is yugF [H]

Identifier: 222526444

GI number: 222526444

Start: 4006793

End: 4008154

Strand: Direct

Name: yugF [H]

Synonym: Chy400_3211

Alternate gene names: 222526444

Gene position: 4006793-4008154 (Clockwise)

Preceding gene: 222526442

Following gene: 222526445

Centisome position: 76.05

GC content: 56.98

Gene sequence:

>1362_bases
ATGGAAATCTTTTCAATCGACGGCGCGAAAATCCATGCCATTGATACCGGGCCGCGTAATGCCCAATTGGCGATCCTGAT
CCATGGCTGGTCAAGTTCATCGTTCGCGATGTCGCCTTTGATTCCACTGTTGAGTCGGCGTTTTCGCTGCATTGCCGTCG
ATCTCCCTGGCTATGGTGAATCGCCACCGTTGCGTGAACGAGCGACAATTGGTCGCTATGCGCAGATCATCGGTCGCCTG
ATAACCGGCTTGAGCGAACATCCAGCGGTGCTGGTCGGTCATTCGATGGGGGGGATGATCAGTGCGACACTGGCGTTGCA
GATTCCCCAGTTAGTTGATCGGATGGTCTTGCTCTGCCCGACCATATCCGGGCGGTTATCATTCTGGATCAACACCTTTG
TCTCACCGATCACCATGCTTGAGCGTTTTCCGCTCTTTAGCCGACTACTCGCGCTGCTTGAGCCGTCGATGCTCTATGTT
ACCGACAGCCTGATGAAACCGGCTTCATTTGCCGAGCGTTCGGCAATCAGCGAGGCCGACTATCTGCGGCTGCGGGCTGA
TGCCCGGCGACCGGGGCAGGGTCGGGTGCGGGCCGAATGTTTTGTGGCGATGCGTAATCAGGATTTGAGCGGACGATTGC
GCGAATTGGAGACGCCGGCGCTGGTTATTTGGGGGGCGGAAGACAATACGGTGCCGTTGCGTGATGCAGGTGTGATCGCC
GATGAATGGCGTAGTGCCGATCTGCGGATTGTGCCAAAAGCCGGACACTGGCCACACTTTGAGACGCCAGACGTGACGCT
GCGCTATATTGCCAGTTACCTGGGTTTACCCTCGATTGTTGGTGAACCGGACATTCTGGTGCAGCCTGCCGAGGTGACGA
CCCAAATTACGGAGTTTCTGGCGAACTCGCAAATCGGTAGCGGTTTGAGTCTGGCTCAACGCACCCGGCTGGCCGCCCAT
TTAGAGCGACGCCGCTATCCACCCGGCACTCTGGTGGATCGCACCAATCAGGAATCGACCGATCTCTATCTGGTGCAGGA
AGGTTCATTAGAGGTCTGGAGTCCGCCGTTGGAGGGCGATCCAGATAGTCAACGCCTGTTGATGACGGTTGTTGCCGGTC
AAATTACCGGTGAGATGTCACTCCTCGATGGGGGCAGGCGAAGTGCCGATCTGCGGGCCGGTGAAGATGGAGTAACGATT
CTGGCCTTGCGACGCGAACGGTTACGGGCACTGGCCGAAGACGATCCGGCGCTTGGAAATGCGGTACTCTGGAATATTGC
TACTGCCCTGGCTTTACGCTTGCGGCTGGCAAACTGGCAACAGCAGGGATTGGTACGTCAGTTACAGGCACTCAGACAGT
AG

Upstream 100 bases:

>100_bases
GCGTACAGCGCTGAAAATCCGGCACAATTGCTGTAGACGAAATAATGCGTATACTGATATGATACCACTAAAAGCGGACA
TAACGCAGCGAGCATGACTT

Downstream 100 bases:

>100_bases
ACATAGCGAGAATAGCATCCTATGGAAACAACAAAGCATCGTTTCAGCGAACTATCGTGGCCTCAGCTTGGATTACTGAT
TGGCGGTGATGGACTGGTGT

Product: cyclic nucleotide-binding protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MEIFSIDGAKIHAIDTGPRNAQLAILIHGWSSSSFAMSPLIPLLSRRFRCIAVDLPGYGESPPLRERATIGRYAQIIGRL
ITGLSEHPAVLVGHSMGGMISATLALQIPQLVDRMVLLCPTISGRLSFWINTFVSPITMLERFPLFSRLLALLEPSMLYV
TDSLMKPASFAERSAISEADYLRLRADARRPGQGRVRAECFVAMRNQDLSGRLRELETPALVIWGAEDNTVPLRDAGVIA
DEWRSADLRIVPKAGHWPHFETPDVTLRYIASYLGLPSIVGEPDILVQPAEVTTQITEFLANSQIGSGLSLAQRTRLAAH
LERRRYPPGTLVDRTNQESTDLYLVQEGSLEVWSPPLEGDPDSQRLLMTVVAGQITGEMSLLDGGRRSADLRAGEDGVTI
LALRRERLRALAEDDPALGNAVLWNIATALALRLRLANWQQQGLVRQLQALRQ

Sequences:

>Translated_453_residues
MEIFSIDGAKIHAIDTGPRNAQLAILIHGWSSSSFAMSPLIPLLSRRFRCIAVDLPGYGESPPLRERATIGRYAQIIGRL
ITGLSEHPAVLVGHSMGGMISATLALQIPQLVDRMVLLCPTISGRLSFWINTFVSPITMLERFPLFSRLLALLEPSMLYV
TDSLMKPASFAERSAISEADYLRLRADARRPGQGRVRAECFVAMRNQDLSGRLRELETPALVIWGAEDNTVPLRDAGVIA
DEWRSADLRIVPKAGHWPHFETPDVTLRYIASYLGLPSIVGEPDILVQPAEVTTQITEFLANSQIGSGLSLAQRTRLAAH
LERRRYPPGTLVDRTNQESTDLYLVQEGSLEVWSPPLEGDPDSQRLLMTVVAGQITGEMSLLDGGRRSADLRAGEDGVTI
LALRRERLRALAEDDPALGNAVLWNIATALALRLRLANWQQQGLVRQLQALRQ
>Mature_453_residues
MEIFSIDGAKIHAIDTGPRNAQLAILIHGWSSSSFAMSPLIPLLSRRFRCIAVDLPGYGESPPLRERATIGRYAQIIGRL
ITGLSEHPAVLVGHSMGGMISATLALQIPQLVDRMVLLCPTISGRLSFWINTFVSPITMLERFPLFSRLLALLEPSMLYV
TDSLMKPASFAERSAISEADYLRLRADARRPGQGRVRAECFVAMRNQDLSGRLRELETPALVIWGAEDNTVPLRDAGVIA
DEWRSADLRIVPKAGHWPHFETPDVTLRYIASYLGLPSIVGEPDILVQPAEVTTQITEFLANSQIGSGLSLAQRTRLAAH
LERRRYPPGTLVDRTNQESTDLYLVQEGSLEVWSPPLEGDPDSQRLLMTVVAGQITGEMSLLDGGRRSADLRAGEDGVTI
LALRRERLRALAEDDPALGNAVLWNIATALALRLRLANWQQQGLVRQLQALRQ

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 49997; Mature: 49997

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIFSIDGAKIHAIDTGPRNAQLAILIHGWSSSSFAMSPLIPLLSRRFRCIAVDLPGYGE
CEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEECCCCCC
SPPLRERATIGRYAQIIGRLITGLSEHPAVLVGHSMGGMISATLALQIPQLVDRMVLLCP
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
TISGRLSFWINTFVSPITMLERFPLFSRLLALLEPSMLYVTDSLMKPASFAERSAISEAD
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHCCCCH
YLRLRADARRPGQGRVRAECFVAMRNQDLSGRLRELETPALVIWGAEDNTVPLRDAGVIA
HEEEECCCCCCCCCCEEHEEEEEECCCCHHHHHHHCCCCEEEEECCCCCEECCCCCCCCH
DEWRSADLRIVPKAGHWPHFETPDVTLRYIASYLGLPSIVGEPDILVQPAEVTTQITEFL
HHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHH
ANSQIGSGLSLAQRTRLAAHLERRRYPPGTLVDRTNQESTDLYLVQEGSLEVWSPPLEGD
HCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCC
PDSQRLLMTVVAGQITGEMSLLDGGRRSADLRAGEDGVTILALRRERLRALAEDDPALGN
CCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCH
AVLWNIATALALRLRLANWQQQGLVRQLQALRQ
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIFSIDGAKIHAIDTGPRNAQLAILIHGWSSSSFAMSPLIPLLSRRFRCIAVDLPGYGE
CEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEECCCCCC
SPPLRERATIGRYAQIIGRLITGLSEHPAVLVGHSMGGMISATLALQIPQLVDRMVLLCP
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
TISGRLSFWINTFVSPITMLERFPLFSRLLALLEPSMLYVTDSLMKPASFAERSAISEAD
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHCCCCH
YLRLRADARRPGQGRVRAECFVAMRNQDLSGRLRELETPALVIWGAEDNTVPLRDAGVIA
HEEEECCCCCCCCCCEEHEEEEEECCCCHHHHHHHCCCCEEEEECCCCCEECCCCCCCCH
DEWRSADLRIVPKAGHWPHFETPDVTLRYIASYLGLPSIVGEPDILVQPAEVTTQITEFL
HHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHH
ANSQIGSGLSLAQRTRLAAHLERRRYPPGTLVDRTNQESTDLYLVQEGSLEVWSPPLEGD
HCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCC
PDSQRLLMTVVAGQITGEMSLLDGGRRSADLRAGEDGVTILALRRERLRALAEDDPALGN
CCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCH
AVLWNIATALALRLRLANWQQQGLVRQLQALRQ
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]