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The map label for this gene is ygcB [C]

Identifier: 222526427

GI number: 222526427

Start: 3952535

End: 3954889

Strand: Direct

Name: ygcB [C]

Synonym: Chy400_3194

Alternate gene names: 222526427

Gene position: 3952535-3954889 (Clockwise)

Preceding gene: 222526426

Following gene: 222526428

Centisome position: 75.02

GC content: 56.6

Gene sequence:

>2355_bases
ATGAAGCCTTTGCCATGGCCTGATTGGCAGGAAGATCTGTTGGCCAAGAGTCAGCAGTACGGCGGAGAGACATTAGCCAG
TCATACGTGGGATGTTCTCTGCCGACTGGCCGATCTCTATCGGTTGCGACCGAACCTCGCAGACCTGGCAGGCGGGGTGC
GCCTGTGGCATTGCCTGTACTGGGCCACCTTTCTCCACGATTTCGGGAAGGCGGCGGCAGGTTTTCAGCGACGGTTGCGT
GGTGGCCCGGCATGGTCGCATCGTCACGAAGTGCTCTCGCTGGCCTTTGTCGATGCGATTGCGCATGATCTGAATGAGTT
AGAACAGCGCTGGGTCGTAGCGGCGATTGTGTCGCACCACCGTGATGCACCGGAAATTCGTGAACTGTACCCTGCCGGGT
TGCGCAAAGATCCGCTAAGCGATCTTTGCGCTGAACTGCAACCGGCTACTGCTGATCATCTGCATCGCTGGCTTGGCGAA
TGCGCGAATCCCTGGCGTATCGAGCTGGGGATGGCGGATGTGGTTGCGGAAATCAGCCCGCAATCACATCCCATTGATGC
CAGACGCATCCGTTACTGGCTATCGGTCTATCACCAGTGGGTAGATATATTGACTGCTGCGGGCACAGCAACCACTGCCC
GTATTCCCGGTATTTTGTTGCGCGGCCTGATCACCACTGCCGATCACATGGCGTCGGCGCATCTCCAGCGGATACCGGAA
CCGATACGCCACTCGTGGCAAGCAGTTATTCCTGCCGGACGGGAAACATATTCTCATCAGGTTGCAAGTGCCGGTGCGAG
TGCGCAGCACGTGTTGTTGATGGCGCCAACCGGTAGTGGCAAGACCGAGGCTGCTTTGGCCTGGGCATTGGGTGATGGCA
GTAACCCACCGGCGCGTATCTTCTACACACTTCCTTATCAGGCCAGTATGAACGCAATGTACGACCGACTATGCGGACTC
TTCGGGAATAATGTTGTTGGTCTTCAGCACGGTCGAGCTGCCCAGGCACTCTACGCCCGTTTCCGGGATGGCGAGGAATG
GTCTGCAACGACGGTGCGCAAGGTACAGTGGGAGAAGAATCTCAACCTCTTACATGCCCGTCCCCTCAAAGTACTCAGTC
CCTACCAACTGCTCAAAGCTCTCTTTCAACTGCGCGGCTTTGAGGCCATGCTGACCGACTATGCACGGGCGGGTTTCATC
TTCGACGAGATTCACGCCTATGAACCAGAACGACTGGCCCTTATCACCGGCTTGATGCGCTATTTGCGCCGGCATGTTGC
GGCCCGCTTCTTCGTGATGTCGGCTACCTTCCCGACTCTCATTCTCGAACAGCTCAGTGATGTGCTGGATACGCCAACTA
TCATTCAGGCCACACCAGACATCTTTGCCCGCTTCCGTCGTCATCAGTTGTACCTGCGTGATGGCGAGCTGACCGATCCT
GCAATTATCACCGAGATTGTCACCGCAGTGCGTGATGGGAAACAGGTTCTGGTCTGTGTCAATACGGTCGCACGGGCGCA
GATGGTGCGTGATCTGCTAATGCAGGCTGGTTTGAGCGACGAGCAACTCATCCTGATCCACAGTCGCTTCACCTATGGAG
ATCGCAATCGGCTGGAGCAGACAATCGTTGAGCGCTGTCGCAGCGATAGCGCCCGGCGGCCACCGTTAGCGCTAGTGGCC
ACCCAGGTGGTCGAAGTTAGCCTCGACATCGATTTGGACACTCTCTACAGTGATCCGGCGCCACTGGAAGCCCTCTTGCA
ACGCTTCGGGCGGGTTAATCGACGGGCAGCGAAAGGAATCTGTCCCGTCTACATCTTCCGTCAGCCCAACGATGGCCAGG
GAGTTTATGGTCGCCATCGTGATCCCGAACAGGCCGGTCACATCGTGCGGGTCACGCTGGCCGAACTCGAACGTCATCAT
GGTGACATTGTCGATGAGTCCGCAGTACAGACCTGGCTGGATACCATCTATGCCGACCCATTGTTGCGTGAGCAATGGTT
GACAGCGTATCAGAACATAAGCCGGCAAGTTGAACAGATCATCAACGGCCTCTGTCCGTTCCAGAGCGATGCCCAGCGTG
AAGATGAATTCGAGCAGATGTTTGACGGTGTGGACGTGGTGCCGGCCTGCTTCGAGCGCGACTATGTCAGTCTGCTGGTC
GAAGAGCGCTTTATCGAGGCGAATGATTATCTGGTCAGCATTAGCAAACAGCGGTTCGCCATCTTACGCAGTCAGGGTAA
GGTGCGACCTGCGGAAGAGACCGGTCAGCGACGAGTCTGGGTTGTGCAAACGCCCTACGACTCGCGTAATGGTCTCTCAT
TTGGCGATACGGTCATTGATCCAGATTGGAGTTAA

Upstream 100 bases:

>100_bases
TTTGGTCAGCAATCGGCAACATACTGGAGTGATCCAACAGCACCGGTTGTTGATGGATTGCCACTCGGTTTGTGGTTTCA
TACCTTTGTGGGAGCAGACG

Downstream 100 bases:

>100_bases
GTATGACACTTACCGTACATCACCTCATCTTCACCGCGACGGCGGAAACCCCGGTTGTCCTGGCGGCGCAAAGTGGGCCA
GCGGTACGCGGTGCGGTTTC

Product: CRISPR-associated helicase Cas3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 784; Mature: 784

Protein sequence:

>784_residues
MKPLPWPDWQEDLLAKSQQYGGETLASHTWDVLCRLADLYRLRPNLADLAGGVRLWHCLYWATFLHDFGKAAAGFQRRLR
GGPAWSHRHEVLSLAFVDAIAHDLNELEQRWVVAAIVSHHRDAPEIRELYPAGLRKDPLSDLCAELQPATADHLHRWLGE
CANPWRIELGMADVVAEISPQSHPIDARRIRYWLSVYHQWVDILTAAGTATTARIPGILLRGLITTADHMASAHLQRIPE
PIRHSWQAVIPAGRETYSHQVASAGASAQHVLLMAPTGSGKTEAALAWALGDGSNPPARIFYTLPYQASMNAMYDRLCGL
FGNNVVGLQHGRAAQALYARFRDGEEWSATTVRKVQWEKNLNLLHARPLKVLSPYQLLKALFQLRGFEAMLTDYARAGFI
FDEIHAYEPERLALITGLMRYLRRHVAARFFVMSATFPTLILEQLSDVLDTPTIIQATPDIFARFRRHQLYLRDGELTDP
AIITEIVTAVRDGKQVLVCVNTVARAQMVRDLLMQAGLSDEQLILIHSRFTYGDRNRLEQTIVERCRSDSARRPPLALVA
TQVVEVSLDIDLDTLYSDPAPLEALLQRFGRVNRRAAKGICPVYIFRQPNDGQGVYGRHRDPEQAGHIVRVTLAELERHH
GDIVDESAVQTWLDTIYADPLLREQWLTAYQNISRQVEQIINGLCPFQSDAQREDEFEQMFDGVDVVPACFERDYVSLLV
EERFIEANDYLVSISKQRFAILRSQGKVRPAEETGQRRVWVVQTPYDSRNGLSFGDTVIDPDWS

Sequences:

>Translated_784_residues
MKPLPWPDWQEDLLAKSQQYGGETLASHTWDVLCRLADLYRLRPNLADLAGGVRLWHCLYWATFLHDFGKAAAGFQRRLR
GGPAWSHRHEVLSLAFVDAIAHDLNELEQRWVVAAIVSHHRDAPEIRELYPAGLRKDPLSDLCAELQPATADHLHRWLGE
CANPWRIELGMADVVAEISPQSHPIDARRIRYWLSVYHQWVDILTAAGTATTARIPGILLRGLITTADHMASAHLQRIPE
PIRHSWQAVIPAGRETYSHQVASAGASAQHVLLMAPTGSGKTEAALAWALGDGSNPPARIFYTLPYQASMNAMYDRLCGL
FGNNVVGLQHGRAAQALYARFRDGEEWSATTVRKVQWEKNLNLLHARPLKVLSPYQLLKALFQLRGFEAMLTDYARAGFI
FDEIHAYEPERLALITGLMRYLRRHVAARFFVMSATFPTLILEQLSDVLDTPTIIQATPDIFARFRRHQLYLRDGELTDP
AIITEIVTAVRDGKQVLVCVNTVARAQMVRDLLMQAGLSDEQLILIHSRFTYGDRNRLEQTIVERCRSDSARRPPLALVA
TQVVEVSLDIDLDTLYSDPAPLEALLQRFGRVNRRAAKGICPVYIFRQPNDGQGVYGRHRDPEQAGHIVRVTLAELERHH
GDIVDESAVQTWLDTIYADPLLREQWLTAYQNISRQVEQIINGLCPFQSDAQREDEFEQMFDGVDVVPACFERDYVSLLV
EERFIEANDYLVSISKQRFAILRSQGKVRPAEETGQRRVWVVQTPYDSRNGLSFGDTVIDPDWS
>Mature_784_residues
MKPLPWPDWQEDLLAKSQQYGGETLASHTWDVLCRLADLYRLRPNLADLAGGVRLWHCLYWATFLHDFGKAAAGFQRRLR
GGPAWSHRHEVLSLAFVDAIAHDLNELEQRWVVAAIVSHHRDAPEIRELYPAGLRKDPLSDLCAELQPATADHLHRWLGE
CANPWRIELGMADVVAEISPQSHPIDARRIRYWLSVYHQWVDILTAAGTATTARIPGILLRGLITTADHMASAHLQRIPE
PIRHSWQAVIPAGRETYSHQVASAGASAQHVLLMAPTGSGKTEAALAWALGDGSNPPARIFYTLPYQASMNAMYDRLCGL
FGNNVVGLQHGRAAQALYARFRDGEEWSATTVRKVQWEKNLNLLHARPLKVLSPYQLLKALFQLRGFEAMLTDYARAGFI
FDEIHAYEPERLALITGLMRYLRRHVAARFFVMSATFPTLILEQLSDVLDTPTIIQATPDIFARFRRHQLYLRDGELTDP
AIITEIVTAVRDGKQVLVCVNTVARAQMVRDLLMQAGLSDEQLILIHSRFTYGDRNRLEQTIVERCRSDSARRPPLALVA
TQVVEVSLDIDLDTLYSDPAPLEALLQRFGRVNRRAAKGICPVYIFRQPNDGQGVYGRHRDPEQAGHIVRVTLAELERHH
GDIVDESAVQTWLDTIYADPLLREQWLTAYQNISRQVEQIINGLCPFQSDAQREDEFEQMFDGVDVVPACFERDYVSLLV
EERFIEANDYLVSISKQRFAILRSQGKVRPAEETGQRRVWVVQTPYDSRNGLSFGDTVIDPDWS

Specific function: Unknown

COG id: COG1203

COG function: function code R; Predicted helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789119, Length=475, Percent_Identity=28.6315789473684, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR006474
- InterPro:   IPR014021 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 88822; Mature: 88822

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLPWPDWQEDLLAKSQQYGGETLASHTWDVLCRLADLYRLRPNLADLAGGVRLWHCLY
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
WATFLHDFGKAAAGFQRRLRGGPAWSHRHEVLSLAFVDAIAHDLNELEQRWVVAAIVSHH
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDAPEIRELYPAGLRKDPLSDLCAELQPATADHLHRWLGECANPWRIELGMADVVAEISP
CCCHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHCCC
QSHPIDARRIRYWLSVYHQWVDILTAAGTATTARIPGILLRGLITTADHMASAHLQRIPE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
PIRHSWQAVIPAGRETYSHQVASAGASAQHVLLMAPTGSGKTEAALAWALGDGSNPPARI
HHHHCHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEE
FYTLPYQASMNAMYDRLCGLFGNNVVGLQHGRAAQALYARFRDGEEWSATTVRKVQWEKN
EEECCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
LNLLHARPLKVLSPYQLLKALFQLRGFEAMLTDYARAGFIFDEIHAYEPERLALITGLMR
CCEEECCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
YLRRHVAARFFVMSATFPTLILEQLSDVLDTPTIIQATPDIFARFRRHQLYLRDGELTDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHEEEEECCCCCCH
AIITEIVTAVRDGKQVLVCVNTVARAQMVRDLLMQAGLSDEQLILIHSRFTYGDRNRLEQ
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHH
TIVERCRSDSARRPPLALVATQVVEVSLDIDLDTLYSDPAPLEALLQRFGRVNRRAAKGI
HHHHHHHCCCCCCCCHHHHHHHHHHEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
CPVYIFRQPNDGQGVYGRHRDPEQAGHIVRVTLAELERHHGDIVDESAVQTWLDTIYADP
CEEEEEECCCCCCCCCCCCCCHHHCCCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHCH
LLREQWLTAYQNISRQVEQIINGLCPFQSDAQREDEFEQMFDGVDVVPACFERDYVSLLV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EERFIEANDYLVSISKQRFAILRSQGKVRPAEETGQRRVWVVQTPYDSRNGLSFGDTVID
HHHHHCCCCEEEEEHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCCCC
PDWS
CCCC
>Mature Secondary Structure
MKPLPWPDWQEDLLAKSQQYGGETLASHTWDVLCRLADLYRLRPNLADLAGGVRLWHCLY
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
WATFLHDFGKAAAGFQRRLRGGPAWSHRHEVLSLAFVDAIAHDLNELEQRWVVAAIVSHH
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDAPEIRELYPAGLRKDPLSDLCAELQPATADHLHRWLGECANPWRIELGMADVVAEISP
CCCHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHCCC
QSHPIDARRIRYWLSVYHQWVDILTAAGTATTARIPGILLRGLITTADHMASAHLQRIPE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
PIRHSWQAVIPAGRETYSHQVASAGASAQHVLLMAPTGSGKTEAALAWALGDGSNPPARI
HHHHCHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEE
FYTLPYQASMNAMYDRLCGLFGNNVVGLQHGRAAQALYARFRDGEEWSATTVRKVQWEKN
EEECCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
LNLLHARPLKVLSPYQLLKALFQLRGFEAMLTDYARAGFIFDEIHAYEPERLALITGLMR
CCEEECCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
YLRRHVAARFFVMSATFPTLILEQLSDVLDTPTIIQATPDIFARFRRHQLYLRDGELTDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHEEEEECCCCCCH
AIITEIVTAVRDGKQVLVCVNTVARAQMVRDLLMQAGLSDEQLILIHSRFTYGDRNRLEQ
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHH
TIVERCRSDSARRPPLALVATQVVEVSLDIDLDTLYSDPAPLEALLQRFGRVNRRAAKGI
HHHHHHHCCCCCCCCHHHHHHHHHHEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
CPVYIFRQPNDGQGVYGRHRDPEQAGHIVRVTLAELERHHGDIVDESAVQTWLDTIYADP
CEEEEEECCCCCCCCCCCCCCHHHCCCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHCH
LLREQWLTAYQNISRQVEQIINGLCPFQSDAQREDEFEQMFDGVDVVPACFERDYVSLLV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EERFIEANDYLVSISKQRFAILRSQGKVRPAEETGQRRVWVVQTPYDSRNGLSFGDTVID
HHHHHCCCCEEEEEHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCCCCC
PDWS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]