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The map label for this gene is usf [H]

Identifier: 222526084

GI number: 222526084

Start: 3505826

End: 3506488

Strand: Direct

Name: usf [H]

Synonym: Chy400_2841

Alternate gene names: 222526084

Gene position: 3505826-3506488 (Clockwise)

Preceding gene: 222526083

Following gene: 222526085

Centisome position: 66.54

GC content: 56.71

Gene sequence:

>663_bases
ATGACAGTTTCGCAACCCCAGGGCTTTCTCGCCGTCCCAACCACCGGAAACGGACCTGGCGTATTGGTGCTGCATGCCTG
GTGGGGGTTAAATGACACCATCAAGATGTACTGCACACAACTTGCCGGGGCCGGTTTCGTAGCCTTCGCCCCAGACCTTT
ATCACGGTAAGGTTACCGACAATATCGCCGAGGCGGAAGCTCTTGCCCAGGCGCTCGACGCCAACCATCGCCAGGTCAGG
GCCGAGATCGTCCAGGCAACGCGGTTTCTTAGCGAACACGCTGACAAAGCTACCCGTGGCCTGGCAGTGATTGGCTTCTC
ACTCGGTGCTTACTATGCGCTTGATCTTTCCGCTACTTGTCCGGAATACATTCGTTCCGTCGTCCTCTTCTATGGCACCG
GTCTCGCCGATTTCAGTAACTCAAAGGCGGCCTATCTCGGTCACTTTGCCGCGAATGACCCATTTGAACCGCAAGCCAAC
ATCTACAGCCTGGAGGATGCCCTCAAGCAGGCTGGCCGTCCGGTGACGTTCTATCATTACAGTGGCACTGGACACTGGTT
CTGTGAACCTGATCGCGATCAGGCGTACAATCCGGCGGCAGCAAGACTGGCCTGGGAGCGAACGCTTCATTTCCTGGAGC
GTGGCACGACTGATGACAGTTAA

Upstream 100 bases:

>100_bases
AAGACCGTCCGACCGGTGCACGATATAGCGGTAGGCCGTCAGTCGTGCACGTTTTCTTTATCCACAGCACGACCTGTTAC
AATCACTGCAGGGAGGCCAC

Downstream 100 bases:

>100_bases
GCTGCCATCGGCGCCTATCTACACCTCGCTTGACTGGAGTGGTTGTTATGCGCATCCAGCACCTGGTGGTTGCGGAAGTT
ATCCTGCTACACGCTAACTG

Product: dienelactone hydrolase

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTVSQPQGFLAVPTTGNGPGVLVLHAWWGLNDTIKMYCTQLAGAGFVAFAPDLYHGKVTDNIAEAEALAQALDANHRQVR
AEIVQATRFLSEHADKATRGLAVIGFSLGAYYALDLSATCPEYIRSVVLFYGTGLADFSNSKAAYLGHFAANDPFEPQAN
IYSLEDALKQAGRPVTFYHYSGTGHWFCEPDRDQAYNPAAARLAWERTLHFLERGTTDDS

Sequences:

>Translated_220_residues
MTVSQPQGFLAVPTTGNGPGVLVLHAWWGLNDTIKMYCTQLAGAGFVAFAPDLYHGKVTDNIAEAEALAQALDANHRQVR
AEIVQATRFLSEHADKATRGLAVIGFSLGAYYALDLSATCPEYIRSVVLFYGTGLADFSNSKAAYLGHFAANDPFEPQAN
IYSLEDALKQAGRPVTFYHYSGTGHWFCEPDRDQAYNPAAARLAWERTLHFLERGTTDDS
>Mature_219_residues
TVSQPQGFLAVPTTGNGPGVLVLHAWWGLNDTIKMYCTQLAGAGFVAFAPDLYHGKVTDNIAEAEALAQALDANHRQVRA
EIVQATRFLSEHADKATRGLAVIGFSLGAYYALDLSATCPEYIRSVVLFYGTGLADFSNSKAAYLGHFAANDPFEPQANI
YSLEDALKQAGRPVTFYHYSGTGHWFCEPDRDQAYNPAAARLAWERTLHFLERGTTDDS

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994982, Length=237, Percent_Identity=25.3164556962025, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 24025; Mature: 23894

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSQPQGFLAVPTTGNGPGVLVLHAWWGLNDTIKMYCTQLAGAGFVAFAPDLYHGKVTD
CCCCCCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCC
NIAEAEALAQALDANHRQVRAEIVQATRFLSEHADKATRGLAVIGFSLGAYYALDLSATC
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCC
PEYIRSVVLFYGTGLADFSNSKAAYLGHFAANDPFEPQANIYSLEDALKQAGRPVTFYHY
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHCCCCEEEEEE
SGTGHWFCEPDRDQAYNPAAARLAWERTLHFLERGTTDDS
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TVSQPQGFLAVPTTGNGPGVLVLHAWWGLNDTIKMYCTQLAGAGFVAFAPDLYHGKVTD
CCCCCCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCC
NIAEAEALAQALDANHRQVRAEIVQATRFLSEHADKATRGLAVIGFSLGAYYALDLSATC
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCC
PEYIRSVVLFYGTGLADFSNSKAAYLGHFAANDPFEPQANIYSLEDALKQAGRPVTFYHY
HHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHCCCCEEEEEE
SGTGHWFCEPDRDQAYNPAAARLAWERTLHFLERGTTDDS
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]