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The map label for this gene is ltaA [H]

Identifier: 222526039

GI number: 222526039

Start: 3444103

End: 3445176

Strand: Reverse

Name: ltaA [H]

Synonym: Chy400_2796

Alternate gene names: 222526039

Gene position: 3445176-3444103 (Counterclockwise)

Preceding gene: 222526052

Following gene: 222526038

Centisome position: 65.39

GC content: 58.85

Gene sequence:

>1074_bases
ATGCAAGGCTATGCTCTGCCTCTCGCTGATCCGCTGCCTGATCGGATCGATCTGCGCAGTGATACAGTAACTTTACCCAG
TCCGGCAATGCGTGCTGCTATGAGTGCAGCGGCACTCGGTGATGATGTCTATGGTGAAGACCCCACAGTCAACGAGCTGG
AACGACTGGCCGCCGAACGAGTTGGCAAAGAAGCCGCGGTACTGGTCGTCAGCGGCACAATGGGGAACCTGGCTGCCATG
CTCGCCCATTGTCAGCGTGGTCAACGGGTGATTCTCGGCAACGAGAGCCACATTTACCACTACGAAGCTGGTGGGGCGAG
TGCGTTGGGTGGACTGATCTACCATCCGCTGTCAACCGATCCCAACGGCTGCATCGATCTGGCAGAACTGGCCGAGGCAG
CAACGACCATCGACGATGCCCACTTTGCGCCTCCGGGGGTTATTTGTCTGGAAAACACGCACAACCGCCGCGGTGGGGTT
GTGCTAACCCCTGATTATCTTGCTCAGGTGCGGCGGATCGCCAATGATCACAGCTTGCCGGTGCACCTTGATGGTGCACG
CATCTTCAATGCCGCCGTTGCCCTGGGTGTACCGGTGACCGACCTCACCAGGCACGTCGATACGGTCATGTTTTGCCTCT
CGAAAGGGCTATCTGCCCCGGTTGGTTCGCTGGTCGCCGGCCCTGCCGATGTGATTGCACGAGTACGTCGGGTGCGCAAA
ATGCTCGGTGGTGGCATGCGTCAAGCGGGGGTGATTGCCGCAGCCGGTATTGTGGCCTTGACCGAGATGGTTGATCGCCT
GGCTGAGGATCACACTAATGCCCGCCTGCTGGCCGCCGGTCTGGCTGAATTGCCGGGTATCCAGATCGATCTGAGCAAAG
TACAAACCAACATCGTGCGCTTCACCTTTGTCCATCCTCGTCTGAGCGTCGAGGCATTCCTGGCCGCCCTACGTAAGCGT
GGCATTCTGATGGGTAGTATGAGCAACACCAGCATCCGGGCTGTGACCCATTACGGCATCGAGGCAGCGCATATTGAACA
GGTGGTTAGCGCAGCGGCTGAGATTCTGGCCTGA

Upstream 100 bases:

>100_bases
ATCTCTCAGCAAAAGCAGTCATACAACCATCATCGCAGAAGCACGTGCTATAATTGGGCAGCAACATTTTTGATTGCCCG
TACACCGGAGGATTCTGGCT

Downstream 100 bases:

>100_bases
TAGATGTTCAACGACACGCCAGGACTGCGATCACCGGTTTCGGGTGTTGTTAGTGAACAGGTCGAGTTGAGAGGAAGCAC
AGCAGGCAGTAGACCGGCAG

Product: Threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MQGYALPLADPLPDRIDLRSDTVTLPSPAMRAAMSAAALGDDVYGEDPTVNELERLAAERVGKEAAVLVVSGTMGNLAAM
LAHCQRGQRVILGNESHIYHYEAGGASALGGLIYHPLSTDPNGCIDLAELAEAATTIDDAHFAPPGVICLENTHNRRGGV
VLTPDYLAQVRRIANDHSLPVHLDGARIFNAAVALGVPVTDLTRHVDTVMFCLSKGLSAPVGSLVAGPADVIARVRRVRK
MLGGGMRQAGVIAAAGIVALTEMVDRLAEDHTNARLLAAGLAELPGIQIDLSKVQTNIVRFTFVHPRLSVEAFLAALRKR
GILMGSMSNTSIRAVTHYGIEAAHIEQVVSAAAEILA

Sequences:

>Translated_357_residues
MQGYALPLADPLPDRIDLRSDTVTLPSPAMRAAMSAAALGDDVYGEDPTVNELERLAAERVGKEAAVLVVSGTMGNLAAM
LAHCQRGQRVILGNESHIYHYEAGGASALGGLIYHPLSTDPNGCIDLAELAEAATTIDDAHFAPPGVICLENTHNRRGGV
VLTPDYLAQVRRIANDHSLPVHLDGARIFNAAVALGVPVTDLTRHVDTVMFCLSKGLSAPVGSLVAGPADVIARVRRVRK
MLGGGMRQAGVIAAAGIVALTEMVDRLAEDHTNARLLAAGLAELPGIQIDLSKVQTNIVRFTFVHPRLSVEAFLAALRKR
GILMGSMSNTSIRAVTHYGIEAAHIEQVVSAAAEILA
>Mature_357_residues
MQGYALPLADPLPDRIDLRSDTVTLPSPAMRAAMSAAALGDDVYGEDPTVNELERLAAERVGKEAAVLVVSGTMGNLAAM
LAHCQRGQRVILGNESHIYHYEAGGASALGGLIYHPLSTDPNGCIDLAELAEAATTIDDAHFAPPGVICLENTHNRRGGV
VLTPDYLAQVRRIANDHSLPVHLDGARIFNAAVALGVPVTDLTRHVDTVMFCLSKGLSAPVGSLVAGPADVIARVRRVRK
MLGGGMRQAGVIAAAGIVALTEMVDRLAEDHTNARLLAAGLAELPGIQIDLSKVQTNIVRFTFVHPRLSVEAFLAALRKR
GILMGSMSNTSIRAVTHYGIEAAHIEQVVSAAAEILA

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=342, Percent_Identity=44.4444444444444, Blast_Score=264, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI193206524, Length=345, Percent_Identity=41.7391304347826, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI193206522, Length=345, Percent_Identity=41.7391304347826, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI193206520, Length=345, Percent_Identity=41.7391304347826, Blast_Score=261, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6320789, Length=342, Percent_Identity=32.7485380116959, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21355439, Length=308, Percent_Identity=45.4545454545455, Blast_Score=251, Evalue=6e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 37624; Mature: 37624

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGYALPLADPLPDRIDLRSDTVTLPSPAMRAAMSAAALGDDVYGEDPTVNELERLAAER
CCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
VGKEAAVLVVSGTMGNLAAMLAHCQRGQRVILGNESHIYHYEAGGASALGGLIYHPLSTD
CCCCEEEEEEECCHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHCCCCCCC
PNGCIDLAELAEAATTIDDAHFAPPGVICLENTHNRRGGVVLTPDYLAQVRRIANDHSLP
CCCCEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECHHHHHHHHHHHCCCCCE
VHLDGARIFNAAVALGVPVTDLTRHVDTVMFCLSKGLSAPVGSLVAGPADVIARVRRVRK
EEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHH
MLGGGMRQAGVIAAAGIVALTEMVDRLAEDHTNARLLAAGLAELPGIQIDLSKVQTNIVR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHHEE
FTFVHPRLSVEAFLAALRKRGILMGSMSNTSIRAVTHYGIEAAHIEQVVSAAAEILA
EEEECCCCCHHHHHHHHHHCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQGYALPLADPLPDRIDLRSDTVTLPSPAMRAAMSAAALGDDVYGEDPTVNELERLAAER
CCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
VGKEAAVLVVSGTMGNLAAMLAHCQRGQRVILGNESHIYHYEAGGASALGGLIYHPLSTD
CCCCEEEEEEECCHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHCCCCCCC
PNGCIDLAELAEAATTIDDAHFAPPGVICLENTHNRRGGVVLTPDYLAQVRRIANDHSLP
CCCCEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECHHHHHHHHHHHCCCCCE
VHLDGARIFNAAVALGVPVTDLTRHVDTVMFCLSKGLSAPVGSLVAGPADVIARVRRVRK
EEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHH
MLGGGMRQAGVIAAAGIVALTEMVDRLAEDHTNARLLAAGLAELPGIQIDLSKVQTNIVR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHHEE
FTFVHPRLSVEAFLAALRKRGILMGSMSNTSIRAVTHYGIEAAHIEQVVSAAAEILA
EEEECCCCCHHHHHHHHHHCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]