Click here to switch to the map view.

The map label for this gene is thrA [C]

Identifier: 222525373

GI number: 222525373

Start: 2691278

End: 2692678

Strand: Direct

Name: thrA [C]

Synonym: Chy400_2118

Alternate gene names: 222525373

Gene position: 2691278-2692678 (Clockwise)

Preceding gene: 222525372

Following gene: 222525377

Centisome position: 51.08

GC content: 60.39

Gene sequence:

>1401_bases
ATGCGTCTGGTCATGAAGTTTGGTGGTACTTCGGTTGGTAGTGCCGATGCAATCCGGCGTGTAACCGAGATTGTTGGTAC
GTATGCCCGCGATCACGAGGTGGTCGTGGTGGTGTCGGCAATGAATGCCCCTGATCTGCGCACGACCGATACTCTGATTG
CAGCGGCGAAAGCGGCAGCGGCGGGAAATGGTGATGCGACTGCGCAAATCGCCCCTCGTCTGCTCGATCTGCATATGCGC
ACAGCAGCAGAGGTGGCATCGCCAGCGGAGTGTGCGGCGCTCGAACCGCAGATCCGTTCCATGCTCGACTACATGAGTAA
CCTTTCACGCAGTATTGCCGTGTTAGGGGAATTGACTCCGCGGGCATTAGACCTGTTTAGCGGTCTTGGCGAACGGCTCA
ATGCGCGCCTGATCGCTGCTGCGCTGCGCAGCGCCGGGATTGATGCCGAGGCGATTGATGCGACTGAACTTATCGTGACC
GATGATCGGTACGGGAATGCCTCGCCTATCGAACCGGATACGCGCGAGCGGAGCCGGGCCAGGCTCTTGCCAATGCTGGC
CGACCGCAAGGTGCCGGTCGTCACCGGTTTCATCGGCGCGACCCGTGCGGGTGTGCCGACGACATTGGGTCGTGGCGGTT
CGGATTACACCTGTGCCATTTTGGGGGCCGATCTCGATGCCGATGAGGTCTGGTTCTGGAAAGAAGTTGACGGCGTCCTT
TCGGCCAACCCGAAGGTAGTGCCGGAAGCCCGAACCTTACCCCGCCTCTCGTATGCCGAGATGGGTGAGATGGCCTATTA
TGGTGCCAACGTCCTCCATCCGAAGACCGTACAGCCGCTGGTGCATCGGCGTATTCCCATTCGCATCCGCAATACCTTCA
ATCCGAGCCATCCGGGAACATTAATCGATGCGGAAGGTGACGGCGAAAGTGTGCGGGCAATCACTGCCATTAGGGGTCTC
AGTCTGATCACGGTAGGTGGGCCGGGTATGCTGGGTCTCACCGGTGTGGCAGCCCGTATCTTCGGCGCAGTTGCCCGCGT
TGGCGCCAACATTCTGCTTATCTCGCAGGCCAGCAGCGAGCAGAGCGTCTGTTTTGCCGTACCAGGTGATGAGTCAGAGC
GGGTGGTAGAAGAACTCCGTCGCGAACTGGCGCGCGAATTTGCGGCCCACGATGTCGATCACATCGGCACCATCGCCCCG
GTCGTGATTGTCGCAGTGGTCGGATCAGGGATGCGGGGCACGCCGGGCATTGCCGGACGGGTGTTTGGTGCGTTGGGTGC
AGCGCGGGTTAACGTGATCGCGATTGCACAGGGTAGCACCGAGAATAACATCTCGCTGGTGGTCGCCGAGGCCGACGCCG
ATGCTGCTGTGCGGGCAGTGCATGCGGCTTTTGTAGGGTAA

Upstream 100 bases:

>100_bases
GTTGCCATACAGTCGGGATTTCTTCCTGGTATCTACGTCGCGGAGATGCGCAGCACAGCGCTCCGCGACGTTTTTTCATA
AGTAGAGAGGAGACAGATCC

Downstream 100 bases:

>100_bases
GCGATCTGTTCGATGAAACAGCATGGCGCGGGTATAGTGCCCGCGCCGGTCTTCATGTTCTTCGCGCCTTGACTGCCAAA
CGTTTAATAGCAGATATAGA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MRLVMKFGGTSVGSADAIRRVTEIVGTYARDHEVVVVVSAMNAPDLRTTDTLIAAAKAAAAGNGDATAQIAPRLLDLHMR
TAAEVASPAECAALEPQIRSMLDYMSNLSRSIAVLGELTPRALDLFSGLGERLNARLIAAALRSAGIDAEAIDATELIVT
DDRYGNASPIEPDTRERSRARLLPMLADRKVPVVTGFIGATRAGVPTTLGRGGSDYTCAILGADLDADEVWFWKEVDGVL
SANPKVVPEARTLPRLSYAEMGEMAYYGANVLHPKTVQPLVHRRIPIRIRNTFNPSHPGTLIDAEGDGESVRAITAIRGL
SLITVGGPGMLGLTGVAARIFGAVARVGANILLISQASSEQSVCFAVPGDESERVVEELRRELAREFAAHDVDHIGTIAP
VVIVAVVGSGMRGTPGIAGRVFGALGAARVNVIAIAQGSTENNISLVVAEADADAAVRAVHAAFVG

Sequences:

>Translated_466_residues
MRLVMKFGGTSVGSADAIRRVTEIVGTYARDHEVVVVVSAMNAPDLRTTDTLIAAAKAAAAGNGDATAQIAPRLLDLHMR
TAAEVASPAECAALEPQIRSMLDYMSNLSRSIAVLGELTPRALDLFSGLGERLNARLIAAALRSAGIDAEAIDATELIVT
DDRYGNASPIEPDTRERSRARLLPMLADRKVPVVTGFIGATRAGVPTTLGRGGSDYTCAILGADLDADEVWFWKEVDGVL
SANPKVVPEARTLPRLSYAEMGEMAYYGANVLHPKTVQPLVHRRIPIRIRNTFNPSHPGTLIDAEGDGESVRAITAIRGL
SLITVGGPGMLGLTGVAARIFGAVARVGANILLISQASSEQSVCFAVPGDESERVVEELRRELAREFAAHDVDHIGTIAP
VVIVAVVGSGMRGTPGIAGRVFGALGAARVNVIAIAQGSTENNISLVVAEADADAAVRAVHAAFVG
>Mature_466_residues
MRLVMKFGGTSVGSADAIRRVTEIVGTYARDHEVVVVVSAMNAPDLRTTDTLIAAAKAAAAGNGDATAQIAPRLLDLHMR
TAAEVASPAECAALEPQIRSMLDYMSNLSRSIAVLGELTPRALDLFSGLGERLNARLIAAALRSAGIDAEAIDATELIVT
DDRYGNASPIEPDTRERSRARLLPMLADRKVPVVTGFIGATRAGVPTTLGRGGSDYTCAILGADLDADEVWFWKEVDGVL
SANPKVVPEARTLPRLSYAEMGEMAYYGANVLHPKTVQPLVHRRIPIRIRNTFNPSHPGTLIDAEGDGESVRAITAIRGL
SLITVGGPGMLGLTGVAARIFGAVARVGANILLISQASSEQSVCFAVPGDESERVVEELRRELAREFAAHDVDHIGTIAP
VVIVAVVGSGMRGTPGIAGRVFGALGAARVNVIAIAQGSTENNISLVVAEADADAAVRAVHAAFVG

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=463, Percent_Identity=34.9892008639309, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1790455, Length=466, Percent_Identity=30.4721030042918, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790376, Length=466, Percent_Identity=27.2532188841202, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6320893, Length=500, Percent_Identity=29, Blast_Score=190, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 48798; Mature: 48798

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVMKFGGTSVGSADAIRRVTEIVGTYARDHEVVVVVSAMNAPDLRTTDTLIAAAKAAA
CEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHC
AGNGDATAQIAPRLLDLHMRTAAEVASPAECAALEPQIRSMLDYMSNLSRSIAVLGELTP
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCH
RALDLFSGLGERLNARLIAAALRSAGIDAEAIDATELIVTDDRYGNASPIEPDTRERSRA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEECCCCCCCCCCCCCHHHHHHH
RLLPMLADRKVPVVTGFIGATRAGVPTTLGRGGSDYTCAILGADLDADEVWFWKEVDGVL
HHHHHHHCCCCCEEEEEHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHHCHHH
SANPKVVPEARTLPRLSYAEMGEMAYYGANVLHPKTVQPLVHRRIPIRIRNTFNPSHPGT
CCCCCCCCCCCCCCCCCHHHHCCHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCE
LIDAEGDGESVRAITAIRGLSLITVGGPGMLGLTGVAARIFGAVARVGANILLISQASSE
EEECCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
QSVCFAVPGDESERVVEELRRELAREFAAHDVDHIGTIAPVVIVAVVGSGMRGTPGIAGR
CCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VFGALGAARVNVIAIAQGSTENNISLVVAEADADAAVRAVHAAFVG
HHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRLVMKFGGTSVGSADAIRRVTEIVGTYARDHEVVVVVSAMNAPDLRTTDTLIAAAKAAA
CEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHC
AGNGDATAQIAPRLLDLHMRTAAEVASPAECAALEPQIRSMLDYMSNLSRSIAVLGELTP
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCH
RALDLFSGLGERLNARLIAAALRSAGIDAEAIDATELIVTDDRYGNASPIEPDTRERSRA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEECCCCCCCCCCCCCHHHHHHH
RLLPMLADRKVPVVTGFIGATRAGVPTTLGRGGSDYTCAILGADLDADEVWFWKEVDGVL
HHHHHHHCCCCCEEEEEHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHHCHHH
SANPKVVPEARTLPRLSYAEMGEMAYYGANVLHPKTVQPLVHRRIPIRIRNTFNPSHPGT
CCCCCCCCCCCCCCCCCHHHHCCHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCE
LIDAEGDGESVRAITAIRGLSLITVGGPGMLGLTGVAARIFGAVARVGANILLISQASSE
EEECCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
QSVCFAVPGDESERVVEELRRELAREFAAHDVDHIGTIAPVVIVAVVGSGMRGTPGIAGR
CCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VFGALGAARVNVIAIAQGSTENNISLVVAEADADAAVRAVHAAFVG
HHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]