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The map label for this gene is wcaK [H]

Identifier: 222524766

GI number: 222524766

Start: 1888791

End: 1890050

Strand: Direct

Name: wcaK [H]

Synonym: Chy400_1493

Alternate gene names: 222524766

Gene position: 1888791-1890050 (Clockwise)

Preceding gene: 222524765

Following gene: 222524767

Centisome position: 35.85

GC content: 52.06

Gene sequence:

>1260_bases
ATGAAGATCGTTTTGATCAATAATCACTCAGTGTTAAATGCTGGTGATTATGCAATTCTGCTCCAGACTCTCAATCTGCT
TCACGCGGCTTTCCCGCAAGCTGATATTAGCATAACCTTCAACGAGCCAGCACTTGCCCGGGCACTGCTCCCAACCTATC
GAATCTACGCTGCACCACCAGCCTGGGGGGTCGAACTCAACCAGCACCGGCAATCTGTTAAGGCATCACGATTTATTCGC
GGATGGTATCTCATCCTCTTACTGATCGGCGCACTCCTGGTACGCTGGACAGGCAAACCAATCCGAGTCTTCCGTGATCC
TCAAAAACAAGCCTTGCTCGCAGAACTGGCTTCAGCCGATCTGGTTGTGGCAAGTGGTGGCGGGTATCTCTACCACTGGT
TCACCTTTGCCTCTGCACTGAGTCTCAGCGCACTTGTGATGCGGCGCAAGCTGGTCATGCTGCCACAATCCATTGGACCA
TTCCATCATCCATTGCAAGTCTGGTTGGCGAGACAGATTGTAGCCAGAGCCGATCTGGTACTCACCCGTGAGTCAATCTC
GCTCCGCCGCGCACAAGATTTGGGAGCAAAAGAAGCACGTTACGAACCAGATCTGGCTTTCGCCTTCCCCTCGGCTCCAG
CCGAAGTGGCCGAGGCATGGTTGCGGTGGCGACTGTCGATACCCAACGCGGCAACAATGTACGTTGGTATTACTGCTATC
AACTGGCAGGGCCAAAATTCACACTTTCGACAACAACAACACTACGAGGAGACATTAGTTGAGTTCATCAATCGTCTAAC
TGACCAGGGTGCGGTAGTTGTCCTGTTTGCCCAGACCTGTGGCCCTGGCCAGGAGGAAGATGACCGACTGGTCAATATGC
GGCTGTATCAAAGGGTGCGACATCAAGCACGAGTCGTGATGCTCAATGAACCCATTCACCCGGCACTGCTCCAGGCACTT
TACGGGTGCATGAATTACTTCGTCGCAACCCGCCTTCACTCTTTTATTTTGGCAACAAATGCCGGCGTACCAGCCCTATC
GATTGGTTATCTGACTAAGAGTATCGGCATTCTATCCGATATGGGTTTACCTGATCGCTGCCTTGACATTCAACATGTGA
ACGTTGCAGATCTCTGGCAGGCATTTTGCCGCTTGCAGCGGGAAGGACTGCCTGATCACATCAAATCCTATATCGCTACG
GCTCGTTCACGTCAGCAAGCGCTCCGGCATGAACTTGCCAGTCGATATGGAAAGGAATAA

Upstream 100 bases:

>100_bases
TCGGTGTCACGGGTGCAACTGCAACGTATATCGTGGTTGAGCTAGCACTTTGTGTGTTCTATTGGATCGGCGCACTCTAC
ACACTGCACAAGACACGCAT

Downstream 100 bases:

>100_bases
AAGTCGGGCTATGGACGGTCGTATCAGTGTGGTTCAATTGATACCTGGCCTGATCGTAGGTGATCTGGGCGGAGGGTTGG
AACTCTACAGTATCCGGCTG

Product: polysaccharide pyruvyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKIVLINNHSVLNAGDYAILLQTLNLLHAAFPQADISITFNEPALARALLPTYRIYAAPPAWGVELNQHRQSVKASRFIR
GWYLILLLIGALLVRWTGKPIRVFRDPQKQALLAELASADLVVASGGGYLYHWFTFASALSLSALVMRRKLVMLPQSIGP
FHHPLQVWLARQIVARADLVLTRESISLRRAQDLGAKEARYEPDLAFAFPSAPAEVAEAWLRWRLSIPNAATMYVGITAI
NWQGQNSHFRQQQHYEETLVEFINRLTDQGAVVVLFAQTCGPGQEEDDRLVNMRLYQRVRHQARVVMLNEPIHPALLQAL
YGCMNYFVATRLHSFILATNAGVPALSIGYLTKSIGILSDMGLPDRCLDIQHVNVADLWQAFCRLQREGLPDHIKSYIAT
ARSRQQALRHELASRYGKE

Sequences:

>Translated_419_residues
MKIVLINNHSVLNAGDYAILLQTLNLLHAAFPQADISITFNEPALARALLPTYRIYAAPPAWGVELNQHRQSVKASRFIR
GWYLILLLIGALLVRWTGKPIRVFRDPQKQALLAELASADLVVASGGGYLYHWFTFASALSLSALVMRRKLVMLPQSIGP
FHHPLQVWLARQIVARADLVLTRESISLRRAQDLGAKEARYEPDLAFAFPSAPAEVAEAWLRWRLSIPNAATMYVGITAI
NWQGQNSHFRQQQHYEETLVEFINRLTDQGAVVVLFAQTCGPGQEEDDRLVNMRLYQRVRHQARVVMLNEPIHPALLQAL
YGCMNYFVATRLHSFILATNAGVPALSIGYLTKSIGILSDMGLPDRCLDIQHVNVADLWQAFCRLQREGLPDHIKSYIAT
ARSRQQALRHELASRYGKE
>Mature_419_residues
MKIVLINNHSVLNAGDYAILLQTLNLLHAAFPQADISITFNEPALARALLPTYRIYAAPPAWGVELNQHRQSVKASRFIR
GWYLILLLIGALLVRWTGKPIRVFRDPQKQALLAELASADLVVASGGGYLYHWFTFASALSLSALVMRRKLVMLPQSIGP
FHHPLQVWLARQIVARADLVLTRESISLRRAQDLGAKEARYEPDLAFAFPSAPAEVAEAWLRWRLSIPNAATMYVGITAI
NWQGQNSHFRQQQHYEETLVEFINRLTDQGAVVVLFAQTCGPGQEEDDRLVNMRLYQRVRHQARVVMLNEPIHPALLQAL
YGCMNYFVATRLHSFILATNAGVPALSIGYLTKSIGILSDMGLPDRCLDIQHVNVADLWQAFCRLQREGLPDHIKSYIAT
ARSRQQALRHELASRYGKE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2327

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide pyruvyl transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788358, Length=384, Percent_Identity=25.2604166666667, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007345 [H]

Pfam domain/function: PF04230 PS_pyruv_trans [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 47214; Mature: 47214

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVLINNHSVLNAGDYAILLQTLNLLHAAFPQADISITFNEPALARALLPTYRIYAAPP
CEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHEEEECCC
AWGVELNQHRQSVKASRFIRGWYLILLLIGALLVRWTGKPIRVFRDPQKQALLAELASAD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCC
LVVASGGGYLYHWFTFASALSLSALVMRRKLVMLPQSIGPFHHPLQVWLARQIVARADLV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHE
LTRESISLRRAQDLGAKEARYEPDLAFAFPSAPAEVAEAWLRWRLSIPNAATMYVGITAI
EEHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHEEECCCCCEEEEEEEEEE
NWQGQNSHFRQQQHYEETLVEFINRLTDQGAVVVLFAQTCGPGQEEDDRLVNMRLYQRVR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
HQARVVMLNEPIHPALLQALYGCMNYFVATRLHSFILATNAGVPALSIGYLTKSIGILSD
HHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHC
MGLPDRCLDIQHVNVADLWQAFCRLQREGLPDHIKSYIATARSRQQALRHELASRYGKE
CCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIVLINNHSVLNAGDYAILLQTLNLLHAAFPQADISITFNEPALARALLPTYRIYAAPP
CEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHEEEECCC
AWGVELNQHRQSVKASRFIRGWYLILLLIGALLVRWTGKPIRVFRDPQKQALLAELASAD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCC
LVVASGGGYLYHWFTFASALSLSALVMRRKLVMLPQSIGPFHHPLQVWLARQIVARADLV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHE
LTRESISLRRAQDLGAKEARYEPDLAFAFPSAPAEVAEAWLRWRLSIPNAATMYVGITAI
EEHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHEEECCCCCEEEEEEEEEE
NWQGQNSHFRQQQHYEETLVEFINRLTDQGAVVVLFAQTCGPGQEEDDRLVNMRLYQRVR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
HQARVVMLNEPIHPALLQALYGCMNYFVATRLHSFILATNAGVPALSIGYLTKSIGILSD
HHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHC
MGLPDRCLDIQHVNVADLWQAFCRLQREGLPDHIKSYIATARSRQQALRHELASRYGKE
CCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503 [H]