Click here to switch to the map view.

The map label for this gene is hpaB [H]

Identifier: 222524742

GI number: 222524742

Start: 1864686

End: 1866281

Strand: Direct

Name: hpaB [H]

Synonym: Chy400_1469

Alternate gene names: 222524742

Gene position: 1864686-1866281 (Clockwise)

Preceding gene: 222524737

Following gene: 222524743

Centisome position: 35.39

GC content: 55.64

Gene sequence:

>1596_bases
ATGACGGTATCCGAAGCGATGACGGAGACTACCGTAGCCGGTGCGCGCCCGATGAACGGGCGTGAGTATCTGGACAGCCT
GCGCGATGATCGAGTGATCTACTTTCAAGGGGAGCGGGTGAAGGATGTTACTACCCATCCGGCGTTTCGCAACTCGGCGC
GTATGGTGGCACGCTGGTATGATCGTCTGCACGAATTGCACCAGGAAGACGTGGCACGAGGTGGGCCTGAAGACTGGAAG
TGGACAATGCCGACCGATACCGGCAGTGGCGGCTGGACACATCCCTTCTTCGTTGGCTCGCGAACGGTTGCCGATCTGGT
GCGGGCGCGAGACACCATTGCCGAACTACAGCGAGCCGTCTACGGCTGGATGGGGCGCGCACCTGATTACAAAGCCGCAT
TTACCGGTACGTTAGGAGCTAATGCTGAATTTTATGCTCCCTACCAGGAAAATGCTCGCCGCTGGTATCGGAAGACCCAG
GAAGAGCTGATTTACTGGAACCACGCCATTGTCAACCCACCGATTGACCGCAACCGGCCTCCAGACGAGGTTCGTGATGT
GTATATGCACGTTGAGCGCGAAACTGATGCCGGTCTAATCGTTTCAGGGGCAAAGGTCGTAGCAACCGGCAGTGCCCTGA
CCCATGTCAATTTTATTGCGCATTACGGGCCGCTCCCCATCAAAGAGAAGCAATTTGCGCTGATCTTCGCCGTTCCCATG
AATGCGCCCGGTATCAAGCTGATCGCTCGCACATCCTACGAGTATAATGCGGCAGTCACCGGCAGTCCCTTCGATTACCC
GCTCTCAAGCCGTCTCGATGAGAACGACTCTATCCTGGTGTTCGATCAAGTCTTGATCCCCTGGGAAAACGTGTTTGTCT
ACGGCGACATTGAGAAGGTCAACACCTTCTTCCCGCTGTCCGGTTTTGGTCATCGCTTCCCACTCCACGGCGGGGTACGT
TTTGCCGTCAAACTTGACTTCATCACCGGCCTGATGCTCAAAGCGGTAGAAGCAACCGGCGTTGCCGAGTTTCGTGGTGT
GCAGGCGCGATTGGGTGAGATTGTCACATATCGCAATCTCTTCTGGCAACTGACAGAGGCGATGGTACGTAACCCGATGC
CGTGGGTTGATGGTTACCTGCTGCCAAATCTCGAAGCTGCCTTCGCGTATCGGGTCATGGCACCGGACGCCTACGTCAAG
ATCAAAGATTTGATCGAGAAAGATGTGGCCAGCGCCTTGATCTATCTCCCTTCGCACGCCGCCGATCTGAAGAACCCCGA
AGTGCGTGGTTACCTGGATCGGTTTGTGCGCGGATCAAACGGCTACAGTGCTCTGGAGCGGATTAAGTTGATGAAACTGC
TCTGGGATGCCATGGGTACCGAATTTGGTGGTCGCCACGAGTTGTACGAGCGGAACTACGCCGGCAATCACGAGAATATC
CGGATCGAGACCCTCGGCGCGGCGATGGCTATGGGTGTAACGGCCCAAATGAAATCGTTTGCCGAACGCTGCATGGCCGA
GTATGACCTCGATGGCTGGACAACGAATGATCTGATCAACCCAACCGATGTCAATGTTGTGATGGGTCAGCATTAG

Upstream 100 bases:

>100_bases
GTGCTCCCCCTATAATCAAAATCACCGCGAAGATAAAAAGCCAGGGATATACCACAACGCGGCAACGGCGCTGGGATTCA
TTTCACAGGGAGGTGAGGTT

Downstream 100 bases:

>100_bases
TTAAGCACAAAGGAGGGATGTATGAGCGAGCCGATCTTTGACACTCACCAGTTAGCCCACGTCGAAATCTTAACGCCCAA
ACCTGATCAGACACTCTGGT

Product: 4-hydroxyphenylacetate 3-hydroxylase

Products: NA

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MTVSEAMTETTVAGARPMNGREYLDSLRDDRVIYFQGERVKDVTTHPAFRNSARMVARWYDRLHELHQEDVARGGPEDWK
WTMPTDTGSGGWTHPFFVGSRTVADLVRARDTIAELQRAVYGWMGRAPDYKAAFTGTLGANAEFYAPYQENARRWYRKTQ
EELIYWNHAIVNPPIDRNRPPDEVRDVYMHVERETDAGLIVSGAKVVATGSALTHVNFIAHYGPLPIKEKQFALIFAVPM
NAPGIKLIARTSYEYNAAVTGSPFDYPLSSRLDENDSILVFDQVLIPWENVFVYGDIEKVNTFFPLSGFGHRFPLHGGVR
FAVKLDFITGLMLKAVEATGVAEFRGVQARLGEIVTYRNLFWQLTEAMVRNPMPWVDGYLLPNLEAAFAYRVMAPDAYVK
IKDLIEKDVASALIYLPSHAADLKNPEVRGYLDRFVRGSNGYSALERIKLMKLLWDAMGTEFGGRHELYERNYAGNHENI
RIETLGAAMAMGVTAQMKSFAERCMAEYDLDGWTTNDLINPTDVNVVMGQH

Sequences:

>Translated_531_residues
MTVSEAMTETTVAGARPMNGREYLDSLRDDRVIYFQGERVKDVTTHPAFRNSARMVARWYDRLHELHQEDVARGGPEDWK
WTMPTDTGSGGWTHPFFVGSRTVADLVRARDTIAELQRAVYGWMGRAPDYKAAFTGTLGANAEFYAPYQENARRWYRKTQ
EELIYWNHAIVNPPIDRNRPPDEVRDVYMHVERETDAGLIVSGAKVVATGSALTHVNFIAHYGPLPIKEKQFALIFAVPM
NAPGIKLIARTSYEYNAAVTGSPFDYPLSSRLDENDSILVFDQVLIPWENVFVYGDIEKVNTFFPLSGFGHRFPLHGGVR
FAVKLDFITGLMLKAVEATGVAEFRGVQARLGEIVTYRNLFWQLTEAMVRNPMPWVDGYLLPNLEAAFAYRVMAPDAYVK
IKDLIEKDVASALIYLPSHAADLKNPEVRGYLDRFVRGSNGYSALERIKLMKLLWDAMGTEFGGRHELYERNYAGNHENI
RIETLGAAMAMGVTAQMKSFAERCMAEYDLDGWTTNDLINPTDVNVVMGQH
>Mature_530_residues
TVSEAMTETTVAGARPMNGREYLDSLRDDRVIYFQGERVKDVTTHPAFRNSARMVARWYDRLHELHQEDVARGGPEDWKW
TMPTDTGSGGWTHPFFVGSRTVADLVRARDTIAELQRAVYGWMGRAPDYKAAFTGTLGANAEFYAPYQENARRWYRKTQE
ELIYWNHAIVNPPIDRNRPPDEVRDVYMHVERETDAGLIVSGAKVVATGSALTHVNFIAHYGPLPIKEKQFALIFAVPMN
APGIKLIARTSYEYNAAVTGSPFDYPLSSRLDENDSILVFDQVLIPWENVFVYGDIEKVNTFFPLSGFGHRFPLHGGVRF
AVKLDFITGLMLKAVEATGVAEFRGVQARLGEIVTYRNLFWQLTEAMVRNPMPWVDGYLLPNLEAAFAYRVMAPDAYVKI
KDLIEKDVASALIYLPSHAADLKNPEVRGYLDRFVRGSNGYSALERIKLMKLLWDAMGTEFGGRHELYERNYAGNHENIR
IETLGAAMAMGVTAQMKSFAERCMAEYDLDGWTTNDLINPTDVNVVMGQH

Specific function: Utilizes FADH(2) supplied by hpaC or by another flavin reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-DHPA. Can also oxidize phenol to catechol, and hydroxylate other phenol derivatives [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012688 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: NA

Molecular weight: Translated: 60078; Mature: 59947

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSEAMTETTVAGARPMNGREYLDSLRDDRVIYFQGERVKDVTTHPAFRNSARMVARWY
CCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCEECCCCCCCHHHHHHHHHHHH
DRLHELHQEDVARGGPEDWKWTMPTDTGSGGWTHPFFVGSRTVADLVRARDTIAELQRAV
HHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
YGWMGRAPDYKAAFTGTLGANAEFYAPYQENARRWYRKTQEELIYWNHAIVNPPIDRNRP
HHHCCCCCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHHHEEEECCEEECCCCCCCCC
PDEVRDVYMHVERETDAGLIVSGAKVVATGSALTHVNFIAHYGPLPIKEKQFALIFAVPM
HHHHHHHHHHEECCCCCCEEEECCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
NAPGIKLIARTSYEYNAAVTGSPFDYPLSSRLDENDSILVFDQVLIPWENVFVYGDIEKV
CCCCEEEEEECCCCCCEEECCCCCCCCHHHCCCCCCCEEEEEEEECCCCCEEEEECHHHH
NTFFPLSGFGHRFPLHGGVRFAVKLDFITGLMLKAVEATGVAEFRGVQARLGEIVTYRNL
CEECCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FWQLTEAMVRNPMPWVDGYLLPNLEAAFAYRVMAPDAYVKIKDLIEKDVASALIYLPSHA
HHHHHHHHHCCCCCCCCCEECCCCHHHHEEEEECCCHHHHHHHHHHHHHHHHEEECCCCH
ADLKNPEVRGYLDRFVRGSNGYSALERIKLMKLLWDAMGTEFGGRHELYERNYAGNHENI
HCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCE
RIETLGAAMAMGVTAQMKSFAERCMAEYDLDGWTTNDLINPTDVNVVMGQH
EEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
>Mature Secondary Structure 
TVSEAMTETTVAGARPMNGREYLDSLRDDRVIYFQGERVKDVTTHPAFRNSARMVARWY
CCHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCEECCCCCCCHHHHHHHHHHHH
DRLHELHQEDVARGGPEDWKWTMPTDTGSGGWTHPFFVGSRTVADLVRARDTIAELQRAV
HHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHH
YGWMGRAPDYKAAFTGTLGANAEFYAPYQENARRWYRKTQEELIYWNHAIVNPPIDRNRP
HHHCCCCCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHHHEEEECCEEECCCCCCCCC
PDEVRDVYMHVERETDAGLIVSGAKVVATGSALTHVNFIAHYGPLPIKEKQFALIFAVPM
HHHHHHHHHHEECCCCCCEEEECCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
NAPGIKLIARTSYEYNAAVTGSPFDYPLSSRLDENDSILVFDQVLIPWENVFVYGDIEKV
CCCCEEEEEECCCCCCEEECCCCCCCCHHHCCCCCCCEEEEEEEECCCCCEEEEECHHHH
NTFFPLSGFGHRFPLHGGVRFAVKLDFITGLMLKAVEATGVAEFRGVQARLGEIVTYRNL
CEECCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FWQLTEAMVRNPMPWVDGYLLPNLEAAFAYRVMAPDAYVKIKDLIEKDVASALIYLPSHA
HHHHHHHHHCCCCCCCCCEECCCCHHHHEEEEECCCHHHHHHHHHHHHHHHHEEECCCCH
ADLKNPEVRGYLDRFVRGSNGYSALERIKLMKLLWDAMGTEFGGRHELYERNYAGNHENI
HCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCE
RIETLGAAMAMGVTAQMKSFAERCMAEYDLDGWTTNDLINPTDVNVVMGQH
EEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10653707 [H]