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The map label for this gene is cadA [H]

Identifier: 222524221

GI number: 222524221

Start: 1187091

End: 1189277

Strand: Reverse

Name: cadA [H]

Synonym: Chy400_0942

Alternate gene names: 222524221

Gene position: 1189277-1187091 (Counterclockwise)

Preceding gene: 222524224

Following gene: 222524218

Centisome position: 22.57

GC content: 60.36

Gene sequence:

>2187_bases
ATGAACAAACAGCAGTTTACGATTAGCGGGATGGATTGCCCGGATTGTGCGCGGAAGATTGAACGGGGAGTCGCACGATT
GCCGGGTGTGACGACGTGCGAGCTGCATTTTACCACTGCCCGCTTGCACGTAGCCGGTGATGTAGATCCGGCGACAGTGA
TTGCGCGGGTACGCGATTTGGGGTACGACATTCAGGCTGAGGCCAGCTCCGGCGATGAGTTACCTCCGACGTTTTTACGT
TTTATGCTAAGCCGCCGGGAGACCCAACTGGCCTTGATCGGTCTGGTGCTGGTATTGCCGGGGATAGTACTCCACGAAGT
GCTCGGTTGGGATGCGTTGTGGATAGAGGTGCTATCGCTGCTGGCGCTGGCACTGGTAGGGCCGGGCATTGCCCGTCAGG
CCTGGCGAACACTCCGTACCAACCGTGAGCTGGGGATTGATGCACTGATGACCATCGCTGCCATCGGGGCGGTGGTGATT
GGCGCCTATGTTGAAGCCGGGCTGGTGCTGGTGCTCTACGCTATTGGCGAGGCGCTTGAAGGGTATACGGCCAATCGTGC
CCGCCACGCTATTCGTAGTCTGCTAGAGCTGACGCCACCGACCGCCACACGGCTGACATCCGGCGGTGAGCAGGTGGTAC
CGGTTGCCGATTTGCAGGTCGGTGATCGGATTCTGATCCGGCCCGGTGAACGGATACCGGTTGATGGCACGATTGTTGCC
GGGAGTTCGCTGGTGAATCAGGCGCCGATCACCGGCGAGAGTCGGCTGGTTGAGCGCGATGTGGGGGATACACTCTTTGC
GGGGACGATGAATGGCGAAGGCAGTATTGAGCTGCGTGTTGATCGCCCCGCCGCCGAGAGTGCGGTGGCCCGGATGATCC
ATCTGGTGCAGGAGGCGCAGGAGCGCCGGGCACCAGTGCAACGGTTTATCGACCGCTTTGCCCGCATCTATACGCCGCTG
GTCACTGCGCTGGCAGCGCTGGTTGCAATCGTACCGCCACTCGTGTTCGGGCAACCGTTCTGGAATCCCGATCCGGATAC
GTTTGGCTGGCTCTACCGTGGTCTGGCACTGCTGGTCGTTGCCTGTCCGTGTGCGCTGGTCATCAGCACGCCGGTCAGTA
TTGTGAGTGCGCTAAGCACAGCGGCCCGGTATGGGGTGCTGATTAAAGGTGGCGCATACCTGGAGACGCTGGCACGGGTA
CAGACGATTGCGCTCGATAAGACCGGTACGCTTACGACCGGTAAACCAACCGTGGTCGGTCTGCGAGCCGTTGGTTGTCC
AGCAGGCGAGGCGACTCCGGTCGGGCATTGCCGTACCTGCGATGAACTGCTTACTATTGCTGGAGCGGTCGAGCGCCGTT
CTGAACATCCCCTTGCCCACGCGATTGTCGGTGCGGCGGTTAATCGTGGTCTTACCCTGCCACCAGCCGAGCAGGTACGG
GCGTTGACCGGGAAAGGCGTGGTGGGGGTGGTCAATGGCGATGAAGTCTTGATCGGGAGTCATCGGGTCTTTGATCAGGC
GTTGCGTCACGATGAATGGCATTGTCTCGCGGCGAAACACGATAGTCAGCTCGGCTACACGCCCCTGATGGTCGGTGTTG
GTGGTCAGTATCGCGGCACGATCACCGTTGCCGATACGGTACGGGCAGAGAGCCGAAGTGCGGTGGCAGCACTCCGTGAT
CAGGGCTTACGCATCGTGATGCTCACCGGTGATGAGCCGGCGACCGCCGAACGGATTGCCGGTGAGATTGGGGTGTCTGA
GGTGCGCGCCGGTCTGTTGCCCGAAACCAAGGCTCAGGTGGTCAGTGCATTACGTCAGTCTGGTGGTGTCGTTGCAATGG
TCGGCGACGGGATCAATGATGCGCCGGCCCTGGCAACCGCTGATGTCGGGATTGCGATGGGGGTGCAGGCCGGGGGCACG
ACGCAGGCTATGGAGACTGCCGATATTACGCTGCTTGGTGGTGATTTGCGGCAGTTGCCGTTTGCAGTAGCACTTAGCCG
GCAGACTATGCGCACGATCACGGCGAATATTGTCTTTAGCATTGGGATCAAGCTGATCTTTATCGTTCTGGTGCTGGCCG
GTCTCAGCACGATGTGGATGGCCGTTTTGGCTGATGTGGGAGCATCGCTGCTGGTCACGCTGAACGGTATGCGCTTGTTG
GGTTTTCGGGCGCGGTTGCCGCAGTAA

Upstream 100 bases:

>100_bases
ACATGGTTTCATTTTCATGCATTCCCCTTGACTCGGCGACAAAATGGCGTAAAATGGAGTCGTAAGCTGAACGTTTTTGT
CCTGCTGAGGAATTTCAACC

Downstream 100 bases:

>100_bases
TATCAAATATTGATGGCCATCGATGTCGCGGCTTGGTAAAGGGGGGGCGGTACGCCGCAAGACGACGGTACGGCGGGTTG
GTGCAGGTAGCGAGCGCGCC

Product: heavy metal translocating P-type ATPase

Products: NA

Alternate protein names: Cadmium efflux ATPase [H]

Number of amino acids: Translated: 728; Mature: 728

Protein sequence:

>728_residues
MNKQQFTISGMDCPDCARKIERGVARLPGVTTCELHFTTARLHVAGDVDPATVIARVRDLGYDIQAEASSGDELPPTFLR
FMLSRRETQLALIGLVLVLPGIVLHEVLGWDALWIEVLSLLALALVGPGIARQAWRTLRTNRELGIDALMTIAAIGAVVI
GAYVEAGLVLVLYAIGEALEGYTANRARHAIRSLLELTPPTATRLTSGGEQVVPVADLQVGDRILIRPGERIPVDGTIVA
GSSLVNQAPITGESRLVERDVGDTLFAGTMNGEGSIELRVDRPAAESAVARMIHLVQEAQERRAPVQRFIDRFARIYTPL
VTALAALVAIVPPLVFGQPFWNPDPDTFGWLYRGLALLVVACPCALVISTPVSIVSALSTAARYGVLIKGGAYLETLARV
QTIALDKTGTLTTGKPTVVGLRAVGCPAGEATPVGHCRTCDELLTIAGAVERRSEHPLAHAIVGAAVNRGLTLPPAEQVR
ALTGKGVVGVVNGDEVLIGSHRVFDQALRHDEWHCLAAKHDSQLGYTPLMVGVGGQYRGTITVADTVRAESRSAVAALRD
QGLRIVMLTGDEPATAERIAGEIGVSEVRAGLLPETKAQVVSALRQSGGVVAMVGDGINDAPALATADVGIAMGVQAGGT
TQAMETADITLLGGDLRQLPFAVALSRQTMRTITANIVFSIGIKLIFIVLVLAGLSTMWMAVLADVGASLLVTLNGMRLL
GFRARLPQ

Sequences:

>Translated_728_residues
MNKQQFTISGMDCPDCARKIERGVARLPGVTTCELHFTTARLHVAGDVDPATVIARVRDLGYDIQAEASSGDELPPTFLR
FMLSRRETQLALIGLVLVLPGIVLHEVLGWDALWIEVLSLLALALVGPGIARQAWRTLRTNRELGIDALMTIAAIGAVVI
GAYVEAGLVLVLYAIGEALEGYTANRARHAIRSLLELTPPTATRLTSGGEQVVPVADLQVGDRILIRPGERIPVDGTIVA
GSSLVNQAPITGESRLVERDVGDTLFAGTMNGEGSIELRVDRPAAESAVARMIHLVQEAQERRAPVQRFIDRFARIYTPL
VTALAALVAIVPPLVFGQPFWNPDPDTFGWLYRGLALLVVACPCALVISTPVSIVSALSTAARYGVLIKGGAYLETLARV
QTIALDKTGTLTTGKPTVVGLRAVGCPAGEATPVGHCRTCDELLTIAGAVERRSEHPLAHAIVGAAVNRGLTLPPAEQVR
ALTGKGVVGVVNGDEVLIGSHRVFDQALRHDEWHCLAAKHDSQLGYTPLMVGVGGQYRGTITVADTVRAESRSAVAALRD
QGLRIVMLTGDEPATAERIAGEIGVSEVRAGLLPETKAQVVSALRQSGGVVAMVGDGINDAPALATADVGIAMGVQAGGT
TQAMETADITLLGGDLRQLPFAVALSRQTMRTITANIVFSIGIKLIFIVLVLAGLSTMWMAVLADVGASLLVTLNGMRLL
GFRARLPQ
>Mature_728_residues
MNKQQFTISGMDCPDCARKIERGVARLPGVTTCELHFTTARLHVAGDVDPATVIARVRDLGYDIQAEASSGDELPPTFLR
FMLSRRETQLALIGLVLVLPGIVLHEVLGWDALWIEVLSLLALALVGPGIARQAWRTLRTNRELGIDALMTIAAIGAVVI
GAYVEAGLVLVLYAIGEALEGYTANRARHAIRSLLELTPPTATRLTSGGEQVVPVADLQVGDRILIRPGERIPVDGTIVA
GSSLVNQAPITGESRLVERDVGDTLFAGTMNGEGSIELRVDRPAAESAVARMIHLVQEAQERRAPVQRFIDRFARIYTPL
VTALAALVAIVPPLVFGQPFWNPDPDTFGWLYRGLALLVVACPCALVISTPVSIVSALSTAARYGVLIKGGAYLETLARV
QTIALDKTGTLTTGKPTVVGLRAVGCPAGEATPVGHCRTCDELLTIAGAVERRSEHPLAHAIVGAAVNRGLTLPPAEQVR
ALTGKGVVGVVNGDEVLIGSHRVFDQALRHDEWHCLAAKHDSQLGYTPLMVGVGGQYRGTITVADTVRAESRSAVAALRD
QGLRIVMLTGDEPATAERIAGEIGVSEVRAGLLPETKAQVVSALRQSGGVVAMVGDGINDAPALATADVGIAMGVQAGGT
TQAMETADITLLGGDLRQLPFAVALSRQTMRTITANIVFSIGIKLIFIVLVLAGLSTMWMAVLADVGASLLVTLNGMRLL
GFRARLPQ

Specific function: This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=785, Percent_Identity=28.5350318471338, Blast_Score=276, Evalue=5e-74,
Organism=Homo sapiens, GI55743073, Length=553, Percent_Identity=29.8372513562387, Blast_Score=217, Evalue=3e-56,
Organism=Homo sapiens, GI115529486, Length=537, Percent_Identity=25.512104283054, Blast_Score=166, Evalue=1e-40,
Organism=Homo sapiens, GI118498343, Length=207, Percent_Identity=28.0193236714976, Blast_Score=77, Evalue=8e-14,
Organism=Homo sapiens, GI48762691, Length=207, Percent_Identity=26.5700483091787, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI48762689, Length=207, Percent_Identity=26.5700483091787, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI48762685, Length=207, Percent_Identity=26.5700483091787, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI48762687, Length=207, Percent_Identity=26.5700483091787, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1789879, Length=731, Percent_Identity=42.8180574555404, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786691, Length=767, Percent_Identity=31.6818774445893, Blast_Score=316, Evalue=3e-87,
Organism=Escherichia coli, GI1786914, Length=491, Percent_Identity=30.5498981670061, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI2367363, Length=185, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556548, Length=595, Percent_Identity=30.9243697478992, Blast_Score=219, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI71997269, Length=176, Percent_Identity=29.5454545454545, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71997262, Length=190, Percent_Identity=28.9473684210526, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71997275, Length=190, Percent_Identity=28.9473684210526, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554158, Length=297, Percent_Identity=23.2323232323232, Blast_Score=66, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71988506, Length=297, Percent_Identity=23.2323232323232, Blast_Score=66, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320475, Length=557, Percent_Identity=33.0341113105925, Blast_Score=257, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6319772, Length=568, Percent_Identity=25.5281690140845, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6325221, Length=639, Percent_Identity=25.508607198748, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321430, Length=633, Percent_Identity=24.4865718799368, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321271, Length=209, Percent_Identity=28.7081339712919, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321432, Length=217, Percent_Identity=28.110599078341, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221329854, Length=583, Percent_Identity=26.5866209262436, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3 [H]

Molecular weight: Translated: 76924; Mature: 76924

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQQFTISGMDCPDCARKIERGVARLPGVTTCELHFTTARLHVAGDVDPATVIARVRDL
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHC
GYDIQAEASSGDELPPTFLRFMLSRRETQLALIGLVLVLPGIVLHEVLGWDALWIEVLSL
CCCEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LALALVGPGIARQAWRTLRTNRELGIDALMTIAAIGAVVIGAYVEAGLVLVLYAIGEALE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYTANRARHAIRSLLELTPPTATRLTSGGEQVVPVADLQVGDRILIRPGERIPVDGTIVA
CCCHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCEEEE
GSSLVNQAPITGESRLVERDVGDTLFAGTMNGEGSIELRVDRPAAESAVARMIHLVQEAQ
CCHHHCCCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
ERRAPVQRFIDRFARIYTPLVTALAALVAIVPPLVFGQPFWNPDPDTFGWLYRGLALLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
ACPCALVISTPVSIVSALSTAARYGVLIKGGAYLETLARVQTIALDKTGTLTTGKPTVVG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHEECCCCCCCCCCCCEEE
LRAVGCPAGEATPVGHCRTCDELLTIAGAVERRSEHPLAHAIVGAAVNRGLTLPPAEQVR
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHH
ALTGKGVVGVVNGDEVLIGSHRVFDQALRHDEWHCLAAKHDSQLGYTPLMVGVGGQYRGT
HHCCCCEEEEECCCEEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCCEEE
ITVADTVRAESRSAVAALRDQGLRIVMLTGDEPATAERIAGEIGVSEVRAGLLPETKAQV
EEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCHHHHH
VSALRQSGGVVAMVGDGINDAPALATADVGIAMGVQAGGTTQAMETADITLLGGDLRQLP
HHHHHHCCCEEEEECCCCCCCCCCEECCCCEEEECCCCCCCHHHHCCCEEEECCCHHHHH
FAVALSRQTMRTITANIVFSIGIKLIFIVLVLAGLSTMWMAVLADVGASLLVTLNGMRLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEE
GFRARLPQ
EHHHCCCC
>Mature Secondary Structure
MNKQQFTISGMDCPDCARKIERGVARLPGVTTCELHFTTARLHVAGDVDPATVIARVRDL
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHC
GYDIQAEASSGDELPPTFLRFMLSRRETQLALIGLVLVLPGIVLHEVLGWDALWIEVLSL
CCCEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LALALVGPGIARQAWRTLRTNRELGIDALMTIAAIGAVVIGAYVEAGLVLVLYAIGEALE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYTANRARHAIRSLLELTPPTATRLTSGGEQVVPVADLQVGDRILIRPGERIPVDGTIVA
CCCHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCEEEE
GSSLVNQAPITGESRLVERDVGDTLFAGTMNGEGSIELRVDRPAAESAVARMIHLVQEAQ
CCHHHCCCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
ERRAPVQRFIDRFARIYTPLVTALAALVAIVPPLVFGQPFWNPDPDTFGWLYRGLALLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
ACPCALVISTPVSIVSALSTAARYGVLIKGGAYLETLARVQTIALDKTGTLTTGKPTVVG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHEECCCCCCCCCCCCEEE
LRAVGCPAGEATPVGHCRTCDELLTIAGAVERRSEHPLAHAIVGAAVNRGLTLPPAEQVR
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHH
ALTGKGVVGVVNGDEVLIGSHRVFDQALRHDEWHCLAAKHDSQLGYTPLMVGVGGQYRGT
HHCCCCEEEEECCCEEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCCCCEEE
ITVADTVRAESRSAVAALRDQGLRIVMLTGDEPATAERIAGEIGVSEVRAGLLPETKAQV
EEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCHHHHH
VSALRQSGGVVAMVGDGINDAPALATADVGIAMGVQAGGTTQAMETADITLLGGDLRQLP
HHHHHHCCCEEEEECCCCCCCCCCEECCCCEEEECCCCCCCHHHHCCCEEEECCCHHHHH
FAVALSRQTMRTITANIVFSIGIKLIFIVLVLAGLSTMWMAVLADVGASLLVTLNGMRLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEE
GFRARLPQ
EHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11302791 [H]