Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 222524106

GI number: 222524106

Start: 1034128

End: 1035543

Strand: Reverse

Name: murD [H]

Synonym: Chy400_0822

Alternate gene names: 222524106

Gene position: 1035543-1034128 (Counterclockwise)

Preceding gene: 222524107

Following gene: 222524105

Centisome position: 19.65

GC content: 60.17

Gene sequence:

>1416_bases
ATGATAGCGGTGGCAGGAAAACGGGTGCTCGTCATGGGGCTTGGGGTTCACGGCGGCGGCCTGGGAGTGGCACGCTGGCT
CTTACGCCATGGTGCCGAAGTGACTGTAACCGATCTCGCTTCGGCTGAGATGCTGGCCGAACCGAAAGCCCAACTTGATG
CATTTGCCGCCGAGATTGGGAAGCAGGTGCGCTACACATTGGGCGAACATCGGGCCGAAGATATTCAGGCCGCCGATCTA
CTGGTAGTTAATCCGGCGGTGCGCCCCGACTCACCCTGGCTGGCGCTCGCCCGGTCGCATGGGGTCAGCATCGAAACCGA
GATGACCCTCTTCTTTCGCGCCTGCCCCGGCCCTATCCTGGGAGTGACCGGCACGAAAGGGAAGACGACCACGACCCTGA
TGCTGGCAGCGATGGTCCGGCAACGCTACCCTGATGCGGTTGTCGCCGGAAACTTGCGCATTTCGGCTTTAGAGGCTCTC
GCACAGATCGGGCCGCAGACACCGGTTGTGCTGGAGCTGAGCAGCTTTCAACTCGAACGACTCGGCGAAGCCGGGTTAAG
CCCGGATTATGCCCTGCTGACCAATCTGGGCATTGACCATCTCAACTGGCACGGCTCGTTTGCGGCCTACGCGAACGCCA
AGCGTCAGATCATGGCGCATCAGGACGAAGATGGGATTGCGGTTTTACCACCAGAGCAGGCCAAAACCTGGGGGCGTGGC
CGGCGCGGAATCATCAGCTTCAGCCTCTCTGATCCAACGGCTGATGCAACGGTTGATGCCGATGGGTATCTGCGCTATCG
TGATGTGATACTTGCGCATCATTCTGATCTGCGCTTACCTGGCACCCACAATCTGGCTAATGCCCTGGCGGCGGCTACGC
TGGCCTACCATTTTGGGATCGAACCGCACGAGATCGTAGCCGCCCTCCAGAGTATGACGGCGGTCGAACACCGGCTGGAG
CTGGTCGCAACGATTGATGGCGTGCGGTATATCAATGATACGACGGCGACGAATCCATCGGCAACAGCGGCTGCATTGGC
GACTGTTGCGGGGCCGATTGTATTGATCGCCGGCGGTGCCGATAAAGACCTTGATCTGGCATACCTGGCGGACACCATAG
CGCAGGGTGCTGCGGCAGTCGTGTTGTTACAGGGAAGTGCAACACCACGATTGCAGACACTCTTGAGCCAGTGCGCACCA
CAATTGCCGCTCGCCGGGCCTTTTGCCGATCTGTCCACCGCGGTAGCAACAGCCCGCTCGCTTGCTCAACCCGGTGGTGC
AATCTTACTTTCGCCCGGATGTGCCAGTTTTGGCATGTTTCGGAATGAGTTTCACCGCGGTGAGGCGTTTCGCCAGATCG
TGCGCGAGTTGGCTGGCGGTCGCGCCGGGGAGCAGAAGTATGACCCGTCCACCTGA

Upstream 100 bases:

>100_bases
TGGTTGGTATCTCGCTGGCGCTGATTTTTGGCCCCCCTGCGACAGGGTTACAGGTCGATCAACCGGGGATCATTGTGATT
GAAGGTGATGGGAGCCGGTA

Downstream 100 bases:

>100_bases
TGAATCCGACGATATTGAAGCATTTCTGCGTCGAGCCGAGCGCGAGTTTGGCCTTCGTCCCGATCTGCCGGAAGAACCTC
CTCCACCTCCACCCCGCCCC

Product: UDP-N-acetylmuramoylalanine/D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MIAVAGKRVLVMGLGVHGGGLGVARWLLRHGAEVTVTDLASAEMLAEPKAQLDAFAAEIGKQVRYTLGEHRAEDIQAADL
LVVNPAVRPDSPWLALARSHGVSIETEMTLFFRACPGPILGVTGTKGKTTTTLMLAAMVRQRYPDAVVAGNLRISALEAL
AQIGPQTPVVLELSSFQLERLGEAGLSPDYALLTNLGIDHLNWHGSFAAYANAKRQIMAHQDEDGIAVLPPEQAKTWGRG
RRGIISFSLSDPTADATVDADGYLRYRDVILAHHSDLRLPGTHNLANALAAATLAYHFGIEPHEIVAALQSMTAVEHRLE
LVATIDGVRYINDTTATNPSATAAALATVAGPIVLIAGGADKDLDLAYLADTIAQGAAAVVLLQGSATPRLQTLLSQCAP
QLPLAGPFADLSTAVATARSLAQPGGAILLSPGCASFGMFRNEFHRGEAFRQIVRELAGGRAGEQKYDPST

Sequences:

>Translated_471_residues
MIAVAGKRVLVMGLGVHGGGLGVARWLLRHGAEVTVTDLASAEMLAEPKAQLDAFAAEIGKQVRYTLGEHRAEDIQAADL
LVVNPAVRPDSPWLALARSHGVSIETEMTLFFRACPGPILGVTGTKGKTTTTLMLAAMVRQRYPDAVVAGNLRISALEAL
AQIGPQTPVVLELSSFQLERLGEAGLSPDYALLTNLGIDHLNWHGSFAAYANAKRQIMAHQDEDGIAVLPPEQAKTWGRG
RRGIISFSLSDPTADATVDADGYLRYRDVILAHHSDLRLPGTHNLANALAAATLAYHFGIEPHEIVAALQSMTAVEHRLE
LVATIDGVRYINDTTATNPSATAAALATVAGPIVLIAGGADKDLDLAYLADTIAQGAAAVVLLQGSATPRLQTLLSQCAP
QLPLAGPFADLSTAVATARSLAQPGGAILLSPGCASFGMFRNEFHRGEAFRQIVRELAGGRAGEQKYDPST
>Mature_471_residues
MIAVAGKRVLVMGLGVHGGGLGVARWLLRHGAEVTVTDLASAEMLAEPKAQLDAFAAEIGKQVRYTLGEHRAEDIQAADL
LVVNPAVRPDSPWLALARSHGVSIETEMTLFFRACPGPILGVTGTKGKTTTTLMLAAMVRQRYPDAVVAGNLRISALEAL
AQIGPQTPVVLELSSFQLERLGEAGLSPDYALLTNLGIDHLNWHGSFAAYANAKRQIMAHQDEDGIAVLPPEQAKTWGRG
RRGIISFSLSDPTADATVDADGYLRYRDVILAHHSDLRLPGTHNLANALAAATLAYHFGIEPHEIVAALQSMTAVEHRLE
LVATIDGVRYINDTTATNPSATAAALATVAGPIVLIAGGADKDLDLAYLADTIAQGAAAVVLLQGSATPRLQTLLSQCAP
QLPLAGPFADLSTAVATARSLAQPGGAILLSPGCASFGMFRNEFHRGEAFRQIVRELAGGRAGEQKYDPST

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=466, Percent_Identity=30.2575107296137, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49638; Mature: 49638

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVAGKRVLVMGLGVHGGGLGVARWLLRHGAEVTVTDLASAEMLAEPKAQLDAFAAEIG
CEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHH
KQVRYTLGEHRAEDIQAADLLVVNPAVRPDSPWLALARSHGVSIETEMTLFFRACPGPIL
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEEEEEEECCCCEE
GVTGTKGKTTTTLMLAAMVRQRYPDAVVAGNLRISALEALAQIGPQTPVVLELSSFQLER
ECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCEEEEECHHHHHH
LGEAGLSPDYALLTNLGIDHLNWHGSFAAYANAKRQIMAHQDEDGIAVLPPEQAKTWGRG
HHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHCCHHHHHEECCCCCEEEECCHHHHHHCCC
RRGIISFSLSDPTADATVDADGYLRYRDVILAHHSDLRLPGTHNLANALAAATLAYHFGI
CCCEEEEEECCCCCCCEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
EPHEIVAALQSMTAVEHRLELVATIDGVRYINDTTATNPSATAAALATVAGPIVLIAGGA
CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCC
DKDLDLAYLADTIAQGAAAVVLLQGSATPRLQTLLSQCAPQLPLAGPFADLSTAVATARS
CCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LAQPGGAILLSPGCASFGMFRNEFHRGEAFRQIVRELAGGRAGEQKYDPST
HCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIAVAGKRVLVMGLGVHGGGLGVARWLLRHGAEVTVTDLASAEMLAEPKAQLDAFAAEIG
CEEECCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHH
KQVRYTLGEHRAEDIQAADLLVVNPAVRPDSPWLALARSHGVSIETEMTLFFRACPGPIL
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEEEEEEECCCCEE
GVTGTKGKTTTTLMLAAMVRQRYPDAVVAGNLRISALEALAQIGPQTPVVLELSSFQLER
ECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCEEEEECHHHHHH
LGEAGLSPDYALLTNLGIDHLNWHGSFAAYANAKRQIMAHQDEDGIAVLPPEQAKTWGRG
HHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHCCHHHHHEECCCCCEEEECCHHHHHHCCC
RRGIISFSLSDPTADATVDADGYLRYRDVILAHHSDLRLPGTHNLANALAAATLAYHFGI
CCCEEEEEECCCCCCCEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
EPHEIVAALQSMTAVEHRLELVATIDGVRYINDTTATNPSATAAALATVAGPIVLIAGGA
CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCC
DKDLDLAYLADTIAQGAAAVVLLQGSATPRLQTLLSQCAPQLPLAGPFADLSTAVATARS
CCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LAQPGGAILLSPGCASFGMFRNEFHRGEAFRQIVRELAGGRAGEQKYDPST
HCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA