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The map label for this gene is ddl [H]

Identifier: 222524098

GI number: 222524098

Start: 1026773

End: 1027843

Strand: Reverse

Name: ddl [H]

Synonym: Chy400_0814

Alternate gene names: 222524098

Gene position: 1027843-1026773 (Counterclockwise)

Preceding gene: 222524099

Following gene: 222524097

Centisome position: 19.51

GC content: 59.48

Gene sequence:

>1071_bases
ATGACCGATTCGATCACCGTGGCCGTCGTGTTTGGTGGGCAAAGTGGCGAGCACGAAGTGTCATTAGTATCAGCCCACGC
CGTAATGAGCAATCTTGATCCGGCGCGCTACCAGATTGAAGCGATCGGTATTGGCAAAGACGGTCAGTGGTGGCATGGGC
CTGGGGCACTGGCAAAATTGATGGCTGAAGCCGATCCTGCCCGGTTGCCGGCCAGTGCGGCGCAGGTTGCCGCGGGGCCG
GTGCATGCGCTTGGGCGCGTTGGTGCGCCGGGATGGGAGCTACCTGCGGCTGATGTCATCTTCCCGGTTTTGCATGGCCC
GTATGGCGAAGATGGCACTATTCAGGGGTTACTTGAACTGGCCGGTCGGCCGTATGTCGGGTGTGGCGTGGCGGCCAGTG
CGGTCGGGATGGACAAAGGTCTGATGAAAGCTGCGTTCGCCGCTGCCGGCCTACCGGTGTTGCCGTGGGTGTTGGTGCGT
CGTAGCGAACTGGCTGATGAGTCTGGCCTGTGTGCCCGGCTTGAGGCTGCCCTGCGCTATCCGATGTTTGTGAAACCGGC
CAACCTCGGCAGTAGCGTAGGAGTGAGTAAAGTTCGTGATCGGGCCGAATTGCTGGCCGGTCTCCACGAAGCGGCTCGCT
ATGATCGGCGGCTTATCGTCGAACAGGGTATCCCGGCACGCGAAATTGAAATCTCGGTGTTGGGGAATGATGAGGTCGTT
GCCAGTATTCCCGGCGAGATCATTCCTGCCGGCGAATGGTATGACTACCACGCCAAGTATATTGACGGCGGTTCGCAGAC
GATAGTACCTGCCGATCTTAGCCCAGCGCAGATCACGCAGGTGCAGGAACTGGCAATGCGGGCTTTTCAGGCCATTGATG
GCGCTGGCCTGGCGCGAGTAGATTTTCTGCTCGACCGCGATCAGGGCACACTTTGGGTGAATGAAGTGAATACGATGCCC
GGGTTTACCCCGATCAGCATGTACCCCAAAATGTGGGCAGCAAGCGGCCTGTCATACGCTCAGTTGCTGGATCGACTGGT
TGATCTGGCACTGGAGCGTGCCAGGCGGTAG

Upstream 100 bases:

>100_bases
TCAGTATAAACGAAACAATAAGCAAAATTGGATCGCCATGCTCGTTGTTGTAGATAACGATCGTCTCACCGATCGATACG
TGTGGTAGAGGAGGCAATAA

Downstream 100 bases:

>100_bases
GAGATAGAGGGCATGGAATACAATCCGCCAAATACTCGTGAACGAATCGTCGCCCGCCGTCAGCGTATGCGGCGAAACAG
CACCGAGCCGGTCGTGCCCG

Product: D-alanine/D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTDSITVAVVFGGQSGEHEVSLVSAHAVMSNLDPARYQIEAIGIGKDGQWWHGPGALAKLMAEADPARLPASAAQVAAGP
VHALGRVGAPGWELPAADVIFPVLHGPYGEDGTIQGLLELAGRPYVGCGVAASAVGMDKGLMKAAFAAAGLPVLPWVLVR
RSELADESGLCARLEAALRYPMFVKPANLGSSVGVSKVRDRAELLAGLHEAARYDRRLIVEQGIPAREIEISVLGNDEVV
ASIPGEIIPAGEWYDYHAKYIDGGSQTIVPADLSPAQITQVQELAMRAFQAIDGAGLARVDFLLDRDQGTLWVNEVNTMP
GFTPISMYPKMWAASGLSYAQLLDRLVDLALERARR

Sequences:

>Translated_356_residues
MTDSITVAVVFGGQSGEHEVSLVSAHAVMSNLDPARYQIEAIGIGKDGQWWHGPGALAKLMAEADPARLPASAAQVAAGP
VHALGRVGAPGWELPAADVIFPVLHGPYGEDGTIQGLLELAGRPYVGCGVAASAVGMDKGLMKAAFAAAGLPVLPWVLVR
RSELADESGLCARLEAALRYPMFVKPANLGSSVGVSKVRDRAELLAGLHEAARYDRRLIVEQGIPAREIEISVLGNDEVV
ASIPGEIIPAGEWYDYHAKYIDGGSQTIVPADLSPAQITQVQELAMRAFQAIDGAGLARVDFLLDRDQGTLWVNEVNTMP
GFTPISMYPKMWAASGLSYAQLLDRLVDLALERARR
>Mature_355_residues
TDSITVAVVFGGQSGEHEVSLVSAHAVMSNLDPARYQIEAIGIGKDGQWWHGPGALAKLMAEADPARLPASAAQVAAGPV
HALGRVGAPGWELPAADVIFPVLHGPYGEDGTIQGLLELAGRPYVGCGVAASAVGMDKGLMKAAFAAAGLPVLPWVLVRR
SELADESGLCARLEAALRYPMFVKPANLGSSVGVSKVRDRAELLAGLHEAARYDRRLIVEQGIPAREIEISVLGNDEVVA
SIPGEIIPAGEWYDYHAKYIDGGSQTIVPADLSPAQITQVQELAMRAFQAIDGAGLARVDFLLDRDQGTLWVNEVNTMPG
FTPISMYPKMWAASGLSYAQLLDRLVDLALERARR

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=359, Percent_Identity=44.5682451253482, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1786280, Length=353, Percent_Identity=35.9773371104816, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 37792; Mature: 37661

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSITVAVVFGGQSGEHEVSLVSAHAVMSNLDPARYQIEAIGIGKDGQWWHGPGALAKL
CCCCEEEEEEECCCCCCCEEEEHHHHHHHHCCCCCCEEEEEEEECCCCCEEECCHHHHHH
MAEADPARLPASAAQVAAGPVHALGRVGAPGWELPAADVIFPVLHGPYGEDGTIQGLLEL
HHCCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
AGRPYVGCGVAASAVGMDKGLMKAAFAAAGLPVLPWVLVRRSELADESGLCARLEAALRY
CCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
PMFVKPANLGSSVGVSKVRDRAELLAGLHEAARYDRRLIVEQGIPAREIEISVLGNDEVV
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
ASIPGEIIPAGEWYDYHAKYIDGGSQTIVPADLSPAQITQVQELAMRAFQAIDGAGLARV
EECCCCEEECCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
DFLLDRDQGTLWVNEVNTMPGFTPISMYPKMWAASGLSYAQLLDRLVDLALERARR
EEEEECCCCEEEEEECCCCCCCCCHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDSITVAVVFGGQSGEHEVSLVSAHAVMSNLDPARYQIEAIGIGKDGQWWHGPGALAKL
CCCEEEEEEECCCCCCCEEEEHHHHHHHHCCCCCCEEEEEEEECCCCCEEECCHHHHHH
MAEADPARLPASAAQVAAGPVHALGRVGAPGWELPAADVIFPVLHGPYGEDGTIQGLLEL
HHCCCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
AGRPYVGCGVAASAVGMDKGLMKAAFAAAGLPVLPWVLVRRSELADESGLCARLEAALRY
CCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
PMFVKPANLGSSVGVSKVRDRAELLAGLHEAARYDRRLIVEQGIPAREIEISVLGNDEVV
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
ASIPGEIIPAGEWYDYHAKYIDGGSQTIVPADLSPAQITQVQELAMRAFQAIDGAGLARV
EECCCCEEECCCCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
DFLLDRDQGTLWVNEVNTMPGFTPISMYPKMWAASGLSYAQLLDRLVDLALERARR
EEEEECCCCEEEEEECCCCCCCCCHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA